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Ajm
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Registered: 08/03/10
Posts: 81
Last seen: 8 years, 11 months
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Re: Lc colonization [Re: Ajm]
#14507905 - 05/25/11 12:38 AM (12 years, 9 months ago) |
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 LC and 7 PF Tek jars inoculated with B+ MS. Will post more pics once any kind of visible growth occurs. I inoculated the center of the PF jars so it might be awhile for those to show any type of growth.
-------------------- " Life is just a ride " --Bill Hicks
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Ajm
Stranger


Registered: 08/03/10
Posts: 81
Last seen: 8 years, 11 months
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Re: Lc colonization [Re: Ajm]
#14520715 - 05/27/11 01:03 PM (12 years, 9 months ago) |
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No growth visible yet in the PF jars. It will probably be another 5-7 days before seeing growth in there, maybe even longer. The LC on the other hand has little black specks in it. I'm guessing it's from the B+ MS syringe, which, relatively, had alot of spores in it. The majority of the specks just stay on the bottom of the jar, when not stirred up. Does this usually occur when using MS for an LC?
-------------------- " Life is just a ride " --Bill Hicks
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: Lc colonization [Re: Ajm]
#14520729 - 05/27/11 01:06 PM (12 years, 9 months ago) |
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I've had it happen, but the spores were only there for 4 days after inoculation, then they made nice mycelium...
Here's an MS LC 1 week after inoculation (strain is Dancing Tiger w/ 1cc of MS solution):

on another note, what's on top of your BRF jars? Are those coffee filters? If so, they're useless...
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Ajm
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Registered: 08/03/10
Posts: 81
Last seen: 8 years, 11 months
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Alright cool maybe it will show mycelium in a few days. Yea, those are coffee filters on top of the jars. Why are they useless?
-------------------- " Life is just a ride " --Bill Hicks
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: Lc colonization [Re: Ajm]
#14520767 - 05/27/11 01:13 PM (12 years, 9 months ago) |
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because coffee filters filter Large particles that you can see w/ your eye, not microscopic organisms like green mold.
It's like trying to stop a mosquito w/ a barbed wire fence.
You should have a dry verm layer on top of your BRF, which is your contam barrier for PF tek.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Ajm
Stranger


Registered: 08/03/10
Posts: 81
Last seen: 8 years, 11 months
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Quote:
todlow said: because coffee filters filter Large particles that you can see w/ your eye, not microscopic organisms like green mold.
It's like trying to stop a mosquito w/ a barbed wire fence.
You should have a dry verm layer on top of your BRF, which is your contam barrier for PF tek.
Haha that's a great analogy. What if the barbed wire fence is enclosing a mosquito zapper mechanism. Then that mosquito is going to be . Yea I have a dry verm layer/mosquito zapper for contams/mosquitos. Thanks for the reply dude, much appreciated.
-------------------- " Life is just a ride " --Bill Hicks
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: Lc colonization [Re: Ajm]
#14520837 - 05/27/11 01:25 PM (12 years, 9 months ago) |
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yea, it's not my analogy...it's RR's. wish I could claim it.
Ditch the coffee filters anyways...
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Ajm
Stranger


Registered: 08/03/10
Posts: 81
Last seen: 8 years, 11 months
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RR, what a monster. Alright, I will take your advice. Will post pics of growth next post, unless someone else posts before the pics.
-------------------- " Life is just a ride " --Bill Hicks
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