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Offlinexrastawarriorx
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Registered: 01/14/11
Posts: 9
Loc: Fl
Last seen: 12 years, 6 months
Is this contamination?
    #14516820 - 05/26/11 06:15 PM (12 years, 8 months ago)

This is my first grow. I have two strains growing in BRF cakes I sterilized in a pressure cooker at 15psi. Thai lipa yai I purchased online and oakridge I received from a friend. The oakridge cakes are doing fine.
My question is about the lipa yai cakes. I inoculated five cakes with the lipa yai about a week and a half ago. All five are growing at about the same pace and are almost fully colonized. I checked on them a few days ago and i noticed some discoloration, but i didnt think anything of it because it looked like moisture build up where the older mycelium is at in the jar.
I checked the jars today and noticed slightly more discoloration. The areas where the older mycelium that first colonized looks more moist than the rest and has a slight orange maybe even brown in some light tint to it.

Please help me out. I dont have a camera that can take a good enough picture to show the discoloration at the moment, but im working on getting one. In the mean time, what do you think I should do? Just boil and toss them out? keep them going to get a pic for further examination? or even attempt to grow them in an outdoor casing?
Any comments will be gratefully appreciated.


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Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: Is this contamination? [Re: xrastawarriorx]
    #14516833 - 05/26/11 06:17 PM (12 years, 8 months ago)

You just described wetspot exactly. Also called blotch, sour rot, bacteria, etc...

Whether or not to use outside depends how nasty they are. If you don't mind messing with em to plant em outside, go for it. Mother nature takes care of the contams.


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The above post is fictional, hypothetical, or downright nonsensical.


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Edited by anonjon (05/26/11 06:18 PM)


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Offlinemabachko
Just Strange
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Registered: 02/11/11
Posts: 702
Last seen: 8 years, 6 months
Re: Is this contamination? [Re: xrastawarriorx]
    #14516855 - 05/26/11 06:20 PM (12 years, 8 months ago)

sounds like myc piss.
Consider them ok for now, but keep an eye on them especially B4 you open them.
When it's time to birth open the worst looking one outdoors. If it has a bad odor toss it.


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Offlinexrastawarriorx
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Male

Registered: 01/14/11
Posts: 9
Loc: Fl
Last seen: 12 years, 6 months
Re: Is this contamination? [Re: anonjon]
    #14527776 - 05/28/11 07:12 PM (12 years, 8 months ago)

I did some research on this and it seems to be correct that it is "sour rot". Would you happen to know how to prevent this in the future? It also seems that my oakridge cakes are beginning to undergo the same problem. Is it because they are taking too long to colonize?


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Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: Is this contamination? [Re: xrastawarriorx]
    #14529561 - 05/29/11 06:26 AM (12 years, 8 months ago)

It's often impossible to know the exact vector of contamination. The possibilities are:

Bad syringe - every injection point would be bad.

Bad technique - some points good / some bad

Too much water - In an ideal world every cake is perfectly sterile, but it's not an ideal world, there's usually some small number of contaminates present. So when you make your cakes too wet, you give any microbes the chance to multiply rapidly.

Incomplete sterilization - contaminates breaking out in small places all over the jar, not just the injection points. There will usually be at least a few small green spots.

Verm barrier failed or got disturbed - contaminates localized to near the top of cake.

Hope that helps.


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The above post is fictional, hypothetical, or downright nonsensical.


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