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Gigabyt3r
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Registered: 05/24/11
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What is LC used for? 1
#14509006 - 05/25/11 09:38 AM (12 years, 8 months ago) |
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I followed the tutorial and it all looks fine and dandy, but what are the mycelium for? Does it speed up the process? If so, how? How are the used in regards to the PF tek? I don't really understand what you do with it then?
Wow thats alot of questions! Thanks in advance
-------------------- "Ummm, you have to be a run-of-the-mill dipshit to use popcorn as your substrate, and sterilize it in the microwave" <3 Shiitake's
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Perun
Mahapralaya...



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Re: What is LC used for? [Re: Gigabyt3r] 1
#14509028 - 05/25/11 09:45 AM (12 years, 8 months ago) |
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Mycelium can be cultured in a liquid (as opposed to a solid like vermiculite/brown rice flour) which you can then use to easily inoculate substrate and start mycelium growth much faster than if you were using spores.
But...LC is a "two bladed sword" you dont know what are you growing untill you test it!
Try this...its much better then any sugar based LC!
http://www.shroomery.org/forums/showflat.php?Cat=&Board=30&Number=6817701&fpart=&PHPSESSID=
-------------------- First: Then:
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runepanther
Im uhh gee!


Registered: 05/17/11
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Re: What is LC used for? [Re: Gigabyt3r] 1
#14509029 - 05/25/11 09:46 AM (12 years, 8 months ago) |
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LC's are basically spore syringes in an advanced stage. It will let the myc spread and colonize much quicker, as it doesnt have to wait to get out of the spore stage. You can use about the same amount of LC as spores in the PF tek. Myc is what would grow if you just dropped spores in the jars... Next time look up what its for before you go about making it and all... Might save you some time in unnecessary things. But in this case the LC will be very useful
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RogerRabbit
Bans for Pleasure



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Re: What is LC used for? [Re: Gigabyt3r] 2
#14509130 - 05/25/11 10:19 AM (12 years, 8 months ago) |
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Quote:
Gigabyt3r said: I followed the tutorial and it all looks fine and dandy, but what are the mycelium for? Does it speed up the process? If so, how? How are the used in regards to the PF tek? I don't really understand what you do with it then?
Wow thats alot of questions! Thanks in advance
Mostly, LC used by noobs to impress other noobs with how much green mold they can grow. I know of exactly zero experienced users who will get anywhere near a liquid culture. It's a waste of time and energy which results in an extremely high contamination rate.
To make matters worse, you don't even know it's contaminated until all that time is wasted and you use it to inoculate your grains or cakes, resulting in even more failure. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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DRCola



Registered: 05/04/11
Posts: 134
Loc: USA
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Hello,
I am simply posting a 10th post on this new thread so I may read the link shown above.
Thanks!
-------------------- Do you need small amounts of high quality Tyvek (sell or trade)? P.M. me!
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slapphappypill
Enthusiast!




Registered: 11/07/10
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Loc: In bed with your mom
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Quote:
RogerRabbit said:
Quote:
Gigabyt3r said: I followed the tutorial and it all looks fine and dandy, but what are the mycelium for? Does it speed up the process? If so, how? How are the used in regards to the PF tek? I don't really understand what you do with it then?
Wow thats alot of questions! Thanks in advance
Mostly, LC used by noobs to impress other noobs with how much green mold they can grow. I know of exactly zero experienced users who will get anywhere near a liquid culture. It's a waste of time and energy which results in an extremely high contamination rate.
To make matters worse, you don't even know it's contaminated until all that time is wasted and you use it to inoculate your grains or cakes, resulting in even more failure. RR
I have to disagree with you Mister Rabbit... I've been using PC since I first started without a single contamination using LC! every one of my cakes has been done with LC and I have never gotten a single contamination! I see those more off of spore syringes than anything else! You know better than anyone else that what works for some doesn't work for others
I've never gotten anything to fruit on rye, yet wbs works perfect. I tried your grain prep method and if I let the steam evap. Off the wbs, it gets too dry and doesn't colonize. If I leave the wbs wet, it colonized perfect in 1-2 weeks.
My point is, some people can whip up a mad crazy LC every time and always get perfect results. Some can't. Don't tell someone not to try something just because it didn't work dlfor you.
And yes, test you PC on a single jar before using it on 50
(PS RR: I never got a response from you when you knocked my tall 1/2 punt and full pint jars)
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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total
Post Office Tyvek Advocator




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I have to disagree with RR as well...  Just because YOU dont use that method, doesnt mean it doesnt work...
I've been doing this for 10 years, so i consider myself an experienced grower...And have used LC's to extend my last bit of spores...
And as a matter of fact, i used to use agar's baby bottle tek along time ago... You calling my man agar a n00b RR? 
(You say that no experienced grower would use a LC due to a high contam risk? I say they arent an experienced grower if they cant grow a clean LC )
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slapphappypill
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Re: What is LC used for? [Re: total] 1
#14509585 - 05/25/11 12:15 PM (12 years, 8 months ago) |
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Quote:
total said: (You say that no experienced grower would use a LC due to a high contam risk? I say they arent an experienced grower if they cant grow a clean LC )

oh and:
LC =
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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Spector
Just Disappeared

Registered: 05/13/11
Posts: 167
Loc: A Scientific Location....
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Re: What is LC used for? [Re: total] 1
#14509674 - 05/25/11 12:33 PM (12 years, 8 months ago) |
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Quote:
total said: (You say that no experienced grower would use a LC due to a high contam risk? I say they arent an experienced grower if they cant grow a clean LC )

Haters gonna hate.
good job guys.  (this post is not to provoke a response, I just totally Agree)
Edited by Spector (05/25/11 01:15 PM)
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total
Post Office Tyvek Advocator




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Re: What is LC used for? [Re: Spector] 1
#14509774 - 05/25/11 12:53 PM (12 years, 8 months ago) |
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I didnt say any of that to insult anyone... I was just sayin'... A LC is a very straight forward and simple thing... If you can successfully innoc a cake/grain jar, you should be able to get a LC jar going...
Most new growers dont have access to agar or more advanced techniques right off the bat, so the methods of duplicating that vendor syringe are few and far between...
But seriously RR... Keep the hate away! I can link you to TC's that use LC's, that you guys appointed as "Knowledgeable and Experienced Growers" 
Go and so you can come back
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slapphappypill
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Re: What is LC used for? [Re: Spector] 1
#14509777 - 05/25/11 12:54 PM (12 years, 8 months ago) |
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Not sure if it was fully answered or not as It seems the other answers are still a little vague. A Liquid Culture (LC) is simply the spores after they start to grow mycelium. Normally you can make an LC with honey water 3-5% ratio pretty easy as that is what I use.
My ONLY issue with LC EVER has been using spores to LC. Sometimes they just don't germinate. and you need to make a new jar. I counter this by making up 3 QT jars of half full LC, and inoculating all three with spores or LC. Then test 3 jars, and 4 days after I see healthy growth with no contams, I knock up the rest of my jars.
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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jimmy8
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I assume RR is referring to professionals as in people who grow mushrooms on a large scale for money. In other words, they can't afford to be spending time testing LCs or risking any unnecessary contamination. I think the fact that RR awarded several people with the TC tag even though he obviously doesn't agree with their methods, is proof enough that he doesn't discriminate. Everyone has a different viewpoint.
I think he speaks so harshly because you can't see what's growing in the LC, so you don't know if your jars are going to get contaminated after inoculation. Regardless of your 99.9% success rate, you can never be sure. I bet even RR and Agar mess up every now and again, so assuming your LC is going to be clean just because it has been every other time prior is a little presumptuous.
However, if you test your LC on a single jar first, that makes things a little less of a crap shoot. You still have to wait for that jar to grow out to test if the LC is clear though.
Just my 20p. I'd be much more comfortable siding with the underdog, but grain LCs are the bomb. If you're an "experienced cultivator" you surely have plenty of spare grain jars around?
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slapphappypill
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Re: What is LC used for? [Re: jimmy8] 1
#14509904 - 05/25/11 01:27 PM (12 years, 8 months ago) |
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I do grain LC as well, but I do Grain LC to honey LC for expansion.
I am also starting my own mushroom farm and will be using multiple methods of grain inoculation including: g2g, LC, agar, GLC. There are different reasons for me doing all 4 at different times depending on my needs at the time.
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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RogerRabbit
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Glad to provoke a response. 
However, if one is going to wait for colonization twice to three times as long for a sugar/karo water with spores injected, as it takes for grains to colonize, and then wait for a test jar to colonize and test, what's the point? During that amount of time you could have colonized a truckload of grains. In addition, you know with grains if they're good or not before you do a transfer. You have no such assurance with liquid culture, especially one made from squirting spore solution in to it.
For those who have never had contamination, all I can do is . With my experience, laminar flow hood, lab suits, gloves, masks, etc., I can make no such claim. I still see contamination from time to time. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Ajm
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I have a Sugar In The Raw LC in colonization stage currently. Colonization of contam, mycelium, or perhaps none, who knows. It was inoculated a little under 24 hours ago with 2ml of B+ MS. From your experience how long does it take to see visible growth, with an MS inoculation, in LC?
-------------------- " Life is just a ride " --Bill Hicks
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: What is LC used for? [Re: Ajm] 1
#14510321 - 05/25/11 03:10 PM (12 years, 8 months ago) |
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Quote:
Ajm said: From your experience how long does it take to see visible growth, with an MS inoculation, in LC?
I hope that questions wasn't directed to RR. If it was 
If not, germination times are about the same as when you inoculate cakes assuming the inoculant is viable and the LC was made properly
--------------------
Quick WBS Prep
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Ajm
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Re: What is LC used for? [Re: FooMan] 1
#14510394 - 05/25/11 03:28 PM (12 years, 8 months ago) |
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Quote:
FooMan said:
Quote:
Ajm said: From your experience how long does it take to see visible growth, with an MS inoculation, in LC?
I hope that questions wasn't directed to RR. If it was 
If not, germination times are about the same as when you inoculate cakes assuming the inoculant is viable and the LC was made properly
It does appear like that. I just meant in general. RR's probably like get outta here dude. Anyways thanks for the reply bro.
-------------------- " Life is just a ride " --Bill Hicks
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slapphappypill
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You can roll your eyes when I say that, but I've never had a contaminated LC. I've seen no growth, but never contams *knock on wood*.
As far as LC goes, it does take longer initially, however once you have a good LC going, you can extend it real far and fly through inoculating qt jars without G2g. I flame sterilize my needle once before getting the LC, once after, then I only use alcohol in between each jar.
And honestly not a single contam ever. Not one 1/2 pint, pint, or QT jar has seen contam'd LC.
Only spore syringes have caused me problems so I start spores on agar now, then take the agar chunk to LC
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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slapphappypill
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Re: What is LC used for? [Re: FooMan] 1
#14510548 - 05/25/11 03:54 PM (12 years, 8 months ago) |
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Quote:
FooMan said:
Quote:
Ajm said: From your experience how long does it take to see visible growth, with an MS inoculation, in LC?
I hope that questions wasn't directed to RR. If it was 
If not, germination times are about the same as when you inoculate cakes assuming the inoculant is viable and the LC was made properly
Yeah, figure about 5-10 days before you can see growth in the LC, and 2 weeks before the LC is ready. I'll test my LC at 2 weeks, but let it sit for another week or so before I put into the fridge
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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Ajm
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Alright cool, thanks man
-------------------- " Life is just a ride " --Bill Hicks
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ShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~




Registered: 07/18/10
Posts: 8,139
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Re: What is LC used for? [Re: total]
#14512213 - 05/25/11 09:49 PM (12 years, 8 months ago) |
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Quote:
total said: I have to disagree with RR as well...  Just because YOU dont use that method, doesnt mean it doesnt work...
I've been doing this for 10 years, so i consider myself an experienced grower...And have used LC's to extend my last bit of spores...
And as a matter of fact, i used to use agar's baby bottle tek along time ago... You calling my man agar a n00b RR? 
(You say that no experienced grower would use a LC due to a high contam risk? I say they arent an experienced grower if they cant grow a clean LC )
 i just made 4 LC's and there all tested and are good
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Doctor_Inoc
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Registered: 04/30/11
Posts: 646
Loc:
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Quote:
As far as LC goes, it does take longer initially, however once you have a good LC going, you can extend it real far and fly through inoculating qt jars without G2g. I flame sterilize my needle once before getting the LC, once after, then I only use alcohol in between each jar.
My biggest issues with LC's are that you can't be 100% sure your LC's clean until test substrates have been inoculated. The other issue is the extra time, steps, and materials needed to make the medium then test it for viability.
I would have transfered that agar culture to quart sized grain jars instead of the LC. At the time it takes to grow out the LC then test it for viability, you could have 10 times the amount of fully colonized grain jars that the amount of culture on agar you begin with would have allowed you to inoculate before or by the time your LC's have been tested for viability.
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slapphappypill
Enthusiast!




Registered: 11/07/10
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Quote:
Doctor_Inoc said:
Quote:
As far as LC goes, it does take longer initially, however once you have a good LC going, you can extend it real far and fly through inoculating qt jars without G2g. I flame sterilize my needle once before getting the LC, once after, then I only use alcohol in between each jar.
My biggest issues with LC's are that you can't be 100% sure your LC's clean until test substrates have been inoculated. The other issue is the extra time, steps, and materials needed to make the medium then test it for viability.
I would have transfered that agar culture to quart sized grain jars instead of the LC. At the time it takes to grow out the LC then test it for viability, you could have 10 times the amount of fully colonized grain jars that the amount of culture on agar you begin with would have allowed you to inoculate before or by the time your LC's have been tested for viability.
True, but my LC lets me use the same myc for a few months. I can split it up to 50 jars one week, 50 jars 2 weeks later, and so on without having to go through my GB or sterilizing my Bathroom as much. LC syringes work great for me right now! as far as commercial growing is concerned, I will eventually start G2G for all my edibles as I'll be doing hundreds of jars at a time and LC wouldn't make sense for that. Spawn bags are also more in m future for spawn prep to inoculate straw logs as well as shiitake beds
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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ShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~




Registered: 07/18/10
Posts: 8,139
Loc: EVERY WHERE
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Quote:
Doctor_Inoc said:
Quote:
As far as LC goes, it does take longer initially, however once you have a good LC going, you can extend it real far and fly through inoculating qt jars without G2g. I flame sterilize my needle once before getting the LC, once after, then I only use alcohol in between each jar.
My biggest issues with LC's are that you can't be 100% sure your LC's clean until test substrates have been inoculated. The other issue is the extra time, steps, and materials needed to make the medium then test it for viability.
I would have transfered that agar culture to quart sized grain jars instead of the LC. At the time it takes to grow out the LC then test it for viability, you could have 10 times the amount of fully colonized grain jars that the amount of culture on agar you begin with would have allowed you to inoculate before or by the time your LC's have been tested for viability.
its not hard for me because i have all the stuff to make the LC,AGAR. i test my lc's on agar and you can tell in just a few days
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ar1es
Psychonaut



Registered: 07/09/10
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in my experience LC is used to grow contams
-------------------- " here is nothing lost or wasted in this life.” - Bhagavad Gita
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ShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~




Registered: 07/18/10
Posts: 8,139
Loc: EVERY WHERE
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Re: What is LC used for? [Re: ar1es]
#14512927 - 05/25/11 11:47 PM (12 years, 8 months ago) |
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Quote:
ar1es said: in my experience LC is used to grow contams
and in mine, i get mushies from my LC
 
@ OP feel free to me if u have any more ?'s
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slapphappypill
Enthusiast!




Registered: 11/07/10
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Quote:
ShadOWCrack said:
Quote:
Doctor_Inoc said:
Quote:
As far as LC goes, it does take longer initially, however once you have a good LC going, you can extend it real far and fly through inoculating qt jars without G2g. I flame sterilize my needle once before getting the LC, once after, then I only use alcohol in between each jar.
My biggest issues with LC's are that you can't be 100% sure your LC's clean until test substrates have been inoculated. The other issue is the extra time, steps, and materials needed to make the medium then test it for viability.
I would have transfered that agar culture to quart sized grain jars instead of the LC. At the time it takes to grow out the LC then test it for viability, you could have 10 times the amount of fully colonized grain jars that the amount of culture on agar you begin with would have allowed you to inoculate before or by the time your LC's have been tested for viability.
its not hard for me because i have all the stuff to make the LC,AGAR. i test my lc's on agar and you can tell in just a few days
I have all the stuff also, just not worth the hassle for me until I get a flow hood (coming soon in a few months as I'm starting a farm and working on filing for my government grant)
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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ShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~




Registered: 07/18/10
Posts: 8,139
Loc: EVERY WHERE
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thor_ak
Mr.



Registered: 04/03/02
Posts: 479
Loc: USA
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Quote:
Gigabyt3r said: I followed the tutorial and it all looks fine and dandy, but what are the mycelium for? Does it speed up the process? If so, how? How are the used in regards to the PF tek? I don't really understand what you do with it then?
Wow thats alot of questions! Thanks in advance
Quote:
runepanther said: Myc is what would grow if you just dropped spores in the jars...
Mycelium is the white stuff in the jars, it consumes your brown rice
flour/vermiculite, and takes nutrients from it to produce mushrooms.
Mycelium comes from spores, once they are moisturized and introduced
into a habitable habitat. Which usually takes between 3-10 days once
you shoot them into your jars VIA syringe. But, if you make a liquid
culture, the mycelium is already growing/living, so when you take that,
and shoot that into your jars VIA syringe, growth will start
immediately. It just speeds up time. If this is your first grow, I
would say just go with the standard syringe/spore tek. Keep it simple
until you understand what you are working with. IMO. Good luck.
-------------------- "A smart man learns from his mistakes. A wise man, learns from the mistakes of others. A woman, makes you pay for these mistakes."
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ar1es
Psychonaut



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Last seen: 1 year, 3 months
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Re: What is LC used for? [Re: thor_ak]
#14515476 - 05/26/11 01:55 PM (12 years, 8 months ago) |
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i see the benefits of LC if you can get it to work
-------------------- " here is nothing lost or wasted in this life.” - Bhagavad Gita
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ar1es
Psychonaut



Registered: 07/09/10
Posts: 776
Last seen: 1 year, 3 months
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Re: What is LC used for? [Re: ar1es]
#14515516 - 05/26/11 02:03 PM (12 years, 8 months ago) |
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anybody ever take a look at this study ?
Developments in the production of mushroom mycelium in submerged liquid culture
Abstract
Yields are high and no special production problems are encountered when mushroom mycelia are grown in submerged liquid culture on simple carbohydrate and nitrogen compounds with mineral salts. Acceptable flavours are obtained with Agaricus campestris, Morchella crassipes, Lepiota rachodes and Coprinus comatus, but these flavours are less pronounced from liquid culture than from solid media.-Univ. Fla, Gainesville.
-------------------- " here is nothing lost or wasted in this life.” - Bhagavad Gita
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
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Re: What is LC used for? [Re: ar1es]
#14515994 - 05/26/11 03:35 PM (12 years, 8 months ago) |
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Yields of what are high? They're not growing mushrooms on LC. It's also about ten percent the speed of grains. There's a reason it's rarely used. I suppose a farm that's growing shiploads of mushrooms might have a use for it, but a small mushroom farm like mine that only turns out a few thousand pounds per month sure as heck doesn't benefit from LC. It's too slow. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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drwatson
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I tried LC for awhile and it worked for me. But when I stepped up to G2G transfers I never looked back. When time is a factor why leave any room for second guessing? You know 100% if a grain jar is good or not immediately upon opening it.
By the time you've made 10 more jars from good spawn with G2G transfer the LC test jars will just be coming back positive or negative (if they're bad you start over).
By the time the viability of LC is confirmed and jars are colonized G2G will have been mixed with a substrate and be well on it's way to giving fruits.
LC might have a place for cloning a specimen maybe. . . but you could just as easily do this with a grain jar too and know 100% that your cloned spawn is viable or not, without the need to make a second test jar.
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drwatson
Slacker



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Re: What is LC used for? [Re: drwatson]
#14516144 - 05/26/11 04:02 PM (12 years, 8 months ago) |
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Oh and to the OP, LC is primarily used to extent the amount of inoculate you have, i.e. Spores from a syringe. A Grain-to-Grain transfer (G2G) is using spawn from a jar you know is good to make more jars thus extending the amount of inoculate you have.
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realcarlos
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Re: What is LC used for? [Re: drwatson]
#14516240 - 05/26/11 04:23 PM (12 years, 8 months ago) |
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What if you use Agar's grain lc tek? Wouldn't you know if its contam or not. That way there is no reason to test it? any thoughts?
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Spector
Just Disappeared

Registered: 05/13/11
Posts: 167
Loc: A Scientific Location....
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Re: What is LC used for? [Re: drwatson]
#14516300 - 05/26/11 04:37 PM (12 years, 8 months ago) |
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to op, What im getting from this is it really matters what stage your at in cultivating mushrooms. yes you could do G2Gs, but that would require a Hood and great technique, Becuase your dealing with sterile grains and cant afford to be wastin every other jar. with Sum of the "poor mans LC teks" all you require is a clean syringe and A hole on your jars, + a glove box.
Its also a great way to expand your Spores so you dont have to keep buying syringes over and over again. yes you could do it this with agar, but again you need to have sum advance items and techniques that most ppl wouldnt have that just cultivate for fun.
Edited by Spector (05/27/11 10:43 AM)
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Perun
Mahapralaya...



Registered: 11/11/10
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Re: What is LC used for? [Re: Spector]
#14519331 - 05/27/11 04:57 AM (12 years, 8 months ago) |
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Quote:
Spector said: What im getting from this is it really matters what stage your at in cultivating mushrooms. yes you could do G2Gs, but that would require a Hood and great technique, Becuase your dealing with sterile grains and cant afford to be wastin every other jar. with Sum of the "poor mans LC teks" all you require is a clean syringe and A hole on your jars, + a glove box.
Its also a great way to expand your Spores so you dont have to keep buying syringes over and over again. yes you could do it this with agar, but again you need to have sum advance items and techniques that most ppl wouldnt have that just cultivate for fun.
G2G does not require a hood! I had been doing g2g in open air for a long time with no contam at all and only started using a glove box for it when i started working with agar,cuz before agar work all my work was done in open air with no contam issues what so ever! All you need is cleen small room,cleen clothes,gloves,mask,lot of air and surface sanitizer and that is it!
-------------------- First: Then:
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realcarlos
Stranger
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Re: What is LC used for? [Re: Perun]
#14519989 - 05/27/11 09:37 AM (12 years, 8 months ago) |
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perun what about grain lcs are these as risky as other lcs?
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FractalXplora
Grainiack




Registered: 02/11/06
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Re: What is LC used for? [Re: realcarlos]
#14520050 - 05/27/11 09:57 AM (12 years, 8 months ago) |
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I like the quickness of a 60ml syringe of LC, you can rip through and knock up 12 or more spawn jars with that once you know its a goodun!
I just wanted to make a home made stir plate too 
But agar wedge too grain is 100% more effective with nearly zero contam rate if your plates are good.
I enjoy both methods.
Also agar wedge to LC is cool as hell and getting it on the stir plate rocks. Nice way to start a Liquid culture.
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total
Post Office Tyvek Advocator




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Re: What is LC used for? [Re: Perun]
#14520103 - 05/27/11 10:21 AM (12 years, 8 months ago) |
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Quote:
Perun said: G2G does not require a hood! I had been doing g2g in open air for a long time with no contam at all

 This is horrible info... Atleast cut 2 holes in a rubbermaid and flip it upside down on a table and use a still air box...
Its no point in risking time/money/energy on a chance that it "might" work...
Take it from a >10yr grower, that is not the way to do things... A $10 still air box is one of the best investments you can make asides from a good pc...
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Spector
Just Disappeared

Registered: 05/13/11
Posts: 167
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Re: What is LC used for? [Re: realcarlos]
#14520248 - 05/27/11 11:01 AM (12 years, 8 months ago) |
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Quote:
realcarlos said: perun what about grain lcs are these as risky as other lcs?
lol carlos, you've been asking and every ones been ignoring you. well i would say with Grain LC's, deponding on the person they are still AS risky as Honey LC's. Yes you confirm in the begining that it is in fact Mycelium. but during the process of moving water To the grain jar, then moving it back to the sterile water jar there are a ton of things that could go wrong with the water and beginners. tho, it is faster and sum what safer then Sugar Lc, were you have to wait then noc up a Jar to make sure its myc.
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realcarlos
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Re: What is LC used for? [Re: Spector]
#14520265 - 05/27/11 11:08 AM (12 years, 8 months ago) |
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lol thanks a million spector
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Ling Ling
Tree Climber


Registered: 10/07/15
Posts: 162
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Re: What is LC used for? [Re: Perun]
#22537358 - 11/17/15 12:31 PM (8 years, 2 months ago) |
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Quote:
Perun said: Mycelium can be cultured in a liquid (as opposed to a solid like vermiculite/brown rice flour) which you can then use to easily inoculate substrate and start mycelium growth much faster than if you were using spores.
But...LC is a "two bladed sword" you dont know what are you growing untill you test it!
Try this...its much better then any sugar based LC!
http://www.shroomery.org/forums/showflat.php?Cat=&Board=30&Number=6817701&fpart=&PHPSESSID=
To view your link I need to be a member for 14 days and have ten posts. I'm only at nine. So this will make it ten.... Sorry for the bump!
-------------------- If any fellow Shroomers have watched the YouTube video "a planned trespass against the mind of man" and are going to pursue your right to live as a man... Or live in Canada under the legal sense of a man (freeman equivalent in the U.S) Please pm me!! Normality is a product of perception!
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
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Re: What is LC used for? [Re: Ling Ling]
#22537737 - 11/17/15 01:51 PM (8 years, 2 months ago) |
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This thread made me grind my teeth
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