Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Kratom Powder for Sale   North Spore Bulk Substrate   Bridgetown Botanicals CBD Concentrates   Left Coast Kratom Buy Kratom Capsules   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1 | 2 | 3  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineGigabyt3r
Stranger

Registered: 05/24/11
Posts: 58
Last seen: 12 years, 6 months
What is LC used for? * 1
    #14509006 - 05/25/11 09:38 AM (12 years, 8 months ago)

I followed the tutorial and it all looks fine and dandy, but what are the mycelium for? Does it speed up the process? If so, how? How are the used in regards to the PF tek? I don't really understand what you do with it then?

Wow thats alot of questions! Thanks in advance


--------------------
"Ummm, you have to be a run-of-the-mill dipshit to use popcorn as your substrate, and sterilize it in the microwave"

<3 Shiitake's


Extras: Filter Print Post Top
OfflinePerun
Mahapralaya...
Male User Gallery


Registered: 11/11/10
Posts: 585
Last seen: 2 years, 2 months
Re: What is LC used for? [Re: Gigabyt3r] * 1
    #14509028 - 05/25/11 09:45 AM (12 years, 8 months ago)

Mycelium can be cultured in a liquid (as opposed to a solid like vermiculite/brown rice flour) which you can then use to easily inoculate substrate and start mycelium growth much faster than if you were using spores.

But...LC is a "two bladed sword" you dont know what are you growing untill you test it!

Try this...its much better then any sugar based LC!

http://www.shroomery.org/forums/showflat.php?Cat=&Board=30&Number=6817701&fpart=&PHPSESSID=


--------------------
First:                                                        Then:


Extras: Filter Print Post Top
Offlinerunepanther
Im uhh gee!
Male

Registered: 05/17/11
Posts: 89
Loc: East ATL, GA
Last seen: 11 years, 1 month
Re: What is LC used for? [Re: Gigabyt3r] * 1
    #14509029 - 05/25/11 09:46 AM (12 years, 8 months ago)

LC's are basically spore syringes in an advanced stage. It will let the myc spread and colonize much quicker, as it doesnt have to wait to get out of the spore stage. You can use about the same amount of LC as spores in the PF tek. Myc is what would grow if you just dropped spores in the jars...
Next time look up what its for before you go about making it and all... Might save you some time in unnecessary things. But in this case the LC will be very useful :smile:


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 4 days
Trusted Cultivator
OG Cultivator
Re: What is LC used for? [Re: Gigabyt3r] * 2
    #14509130 - 05/25/11 10:19 AM (12 years, 8 months ago)

Quote:

Gigabyt3r said:
I followed the tutorial and it all looks fine and dandy, but what are the mycelium for? Does it speed up the process? If so, how? How are the used in regards to the PF tek? I don't really understand what you do with it then?

Wow thats alot of questions! Thanks in advance




Mostly, LC used by noobs to impress other noobs with how much green mold they can grow.  I know of exactly zero experienced users who will get anywhere near a liquid culture.  It's a waste of time and energy which results in an extremely high contamination rate.

To make matters worse, you don't even know it's contaminated until all that time is wasted and you use it to inoculate your grains or cakes, resulting in even more failure.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
InvisibleDRCola
Male User Gallery


Registered: 05/04/11
Posts: 134
Loc: USA Flag
Re: What is LC used for? [Re: RogerRabbit] * 1
    #14509263 - 05/25/11 11:03 AM (12 years, 8 months ago)

Hello,

I am simply posting a 10th post on this new thread so I may read the link shown above.

Thanks!


--------------------
Do you need small amounts of high quality Tyvek (sell or trade)? P.M. me!


Extras: Filter Print Post Top
Invisibleslapphappypill
Enthusiast!
 User Gallery


Folding@home Statistics
Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
Re: What is LC used for? [Re: RogerRabbit] * 1
    #14509335 - 05/25/11 11:19 AM (12 years, 8 months ago)

Quote:

RogerRabbit said:
Quote:

Gigabyt3r said:
I followed the tutorial and it all looks fine and dandy, but what are the mycelium for? Does it speed up the process? If so, how? How are the used in regards to the PF tek? I don't really understand what you do with it then?

Wow thats alot of questions! Thanks in advance




Mostly, LC used by noobs to impress other noobs with how much green mold they can grow.  I know of exactly zero experienced users who will get anywhere near a liquid culture.  It's a waste of time and energy which results in an extremely high contamination rate.

To make matters worse, you don't even know it's contaminated until all that time is wasted and you use it to inoculate your grains or cakes, resulting in even more failure.
RR




I have to disagree with you Mister Rabbit... I've been using PC since I first started without a single contamination using LC!
every one of my cakes has been done with LC and I have never gotten a single contamination! I see those more off of spore syringes than anything else! You know better than anyone else that what works for some doesn't work for others

I've never gotten anything to fruit on rye, yet wbs works perfect. I tried your grain prep method and if I let the steam evap. Off the wbs, it gets too dry and doesn't colonize. If I leave the wbs wet, it colonized perfect in 1-2 weeks.

My point is, some people can whip up a mad crazy LC every time and always get perfect results. Some can't. Don't tell someone not to try something just because it didn't work dlfor you. 

And yes, test you PC on a single jar before using it on 50


(PS RR: I never got a response from you when you knocked my tall 1/2 punt and full pint jars)


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


Extras: Filter Print Post Top
Offlinetotal
Post Office Tyvek Advocator
Male User Gallery


Folding@home Statistics
Registered: 10/03/04
Posts: 11,405
Last seen: 6 months, 24 days
Trusted Cultivator
Re: What is LC used for? [Re: slapphappypill] * 1
    #14509373 - 05/25/11 11:27 AM (12 years, 8 months ago)

I have to disagree with RR as well... :shrug:
Just because YOU dont use that method, doesnt mean it doesnt work...

I've been doing this for 10 years, so i consider myself an experienced grower...And have used LC's to extend my last bit of spores...

And as a matter of fact, i used to use agar's baby bottle tek along time ago...
You calling my man agar a n00b RR? :oldman:

(You say that no experienced grower would use a LC due to a high contam risk?  I say they arent an experienced grower if they cant grow a clean LC :shrug:)


Extras: Filter Print Post Top
Invisibleslapphappypill
Enthusiast!
 User Gallery


Folding@home Statistics
Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
Re: What is LC used for? [Re: total] * 1
    #14509585 - 05/25/11 12:15 PM (12 years, 8 months ago)

Quote:

total said:
(You say that no experienced grower would use a LC due to a high contam risk?  I say they arent an experienced grower if they cant grow a clean LC :shrug:)




:whathesaid:

oh and:

LC = :winning:


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


Extras: Filter Print Post Top
InvisibleSpector
Just Disappeared

Registered: 05/13/11
Posts: 167
Loc: A Scientific Location....
Re: What is LC used for? [Re: total] * 1
    #14509674 - 05/25/11 12:33 PM (12 years, 8 months ago)

Quote:

total said:
(You say that no experienced grower would use a LC due to a high contam risk?  I say they arent an experienced grower if they cant grow a clean LC :shrug:)



:burn:

Haters gonna hate.

good job guys. :thumbup:
(this post is not to provoke a response, I just totally Agree)


Edited by Spector (05/25/11 01:15 PM)


Extras: Filter Print Post Top
Offlinetotal
Post Office Tyvek Advocator
Male User Gallery


Folding@home Statistics
Registered: 10/03/04
Posts: 11,405
Last seen: 6 months, 24 days
Trusted Cultivator
Re: What is LC used for? [Re: Spector] * 1
    #14509774 - 05/25/11 12:53 PM (12 years, 8 months ago)

I didnt say any of that to insult anyone...
I was just sayin'...
A LC is a very straight forward and simple thing...
If you can successfully innoc a cake/grain jar, you should be able to get a LC jar going...:shrug:

Most new growers dont have access to agar or more advanced techniques right off the bat, so the methods of duplicating that vendor syringe are few and far between...

But seriously RR...
Keep the hate away! I can link you to TC's that use LC's, that you guys appointed as "Knowledgeable and Experienced Growers" :super:

Go :doggystyle: and :bongload: so you can come back  :omgawesome:


Extras: Filter Print Post Top
Invisibleslapphappypill
Enthusiast!
 User Gallery


Folding@home Statistics
Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
Re: What is LC used for? [Re: Spector] * 1
    #14509777 - 05/25/11 12:54 PM (12 years, 8 months ago)

Not sure if it was fully answered or not as It seems the other answers are still a little vague. A Liquid Culture (LC) is simply the spores after they start to grow mycelium. Normally you can make an LC with honey water 3-5% ratio pretty easy as that is what I use.

My ONLY issue with LC EVER has been using spores to LC. Sometimes they just don't germinate. and you need to make a new jar. I counter this by making up 3 QT jars of half full LC, and inoculating all three with spores or LC. Then test 3 jars, and 4 days after I see healthy growth with no contams, I knock up the rest of my jars.


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


Extras: Filter Print Post Top
Offlinejimmy8
Stranger
Male

Registered: 02/12/10
Posts: 48
Loc: UK Flag
Last seen: 12 years, 7 months
Re: What is LC used for? [Re: slapphappypill] * 2
    #14509883 - 05/25/11 01:22 PM (12 years, 8 months ago)

I assume RR is referring to professionals as in people who grow mushrooms on a large scale for money. In other words, they can't afford to be spending time testing LCs or risking any unnecessary contamination. I think the fact that RR awarded several people with the TC tag even though he obviously doesn't agree with their methods, is proof enough that he doesn't discriminate. Everyone has a different viewpoint.

I think he speaks so harshly because you can't see what's growing in the LC, so you don't know if your jars are going to get contaminated after inoculation. Regardless of your 99.9% success rate, you can never be sure. I bet even RR and Agar mess up every now and again, so assuming your LC is going to be clean just because it has been every other time prior is a little presumptuous.

However, if you test your LC on a single jar first, that makes things a little less of a crap shoot. You still have to wait for that jar to grow out to test if the LC is clear though.

Just my 20p. I'd be much more comfortable siding with the underdog, but grain LCs are the bomb. If you're an "experienced cultivator" you surely have plenty of spare grain jars around? :wink:


Extras: Filter Print Post Top
Invisibleslapphappypill
Enthusiast!
 User Gallery


Folding@home Statistics
Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
Re: What is LC used for? [Re: jimmy8] * 1
    #14509904 - 05/25/11 01:27 PM (12 years, 8 months ago)

I do grain LC as well, but I do Grain LC to honey LC for expansion.

I am also starting my own mushroom farm and will be using multiple methods of grain inoculation including: g2g, LC, agar, GLC. There are different reasons for me doing all 4 at different times depending on my needs at the time.


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 4 days
Trusted Cultivator
OG Cultivator
Re: What is LC used for? [Re: slapphappypill] * 2
    #14510141 - 05/25/11 02:26 PM (12 years, 8 months ago)

Glad to provoke a response. :thumbup:

However, if one is going to wait for colonization twice to three times as long for a sugar/karo water with spores injected, as it takes for grains to colonize, and then wait for a test jar to colonize and test, what's the point?  During that amount of time you could have colonized a truckload of grains.  In addition, you know with grains if they're good or not before you do a transfer.  You have no such assurance with liquid culture, especially one made from squirting spore solution in to it.

For those who have never had contamination, all I can do is :rolleyes:.  With my experience, laminar flow hood, lab suits, gloves, masks, etc., I can make no such claim.  I still see contamination from time to time.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
OfflineAjm
Stranger
Male User Gallery

Registered: 08/03/10
Posts: 81
Last seen: 8 years, 10 months
Re: What is LC used for? [Re: RogerRabbit] * 1
    #14510276 - 05/25/11 02:55 PM (12 years, 8 months ago)

I have a Sugar In The Raw LC in colonization stage currently. Colonization of contam, mycelium, or perhaps none, who knows. It was inoculated a little under 24 hours ago with 2ml of B+ MS. From your experience how long does it take to see visible growth, with an MS inoculation, in LC? 
:rockon:  :hairmetal:  :drumming:


--------------------
" Life is just a ride " --Bill Hicks


Extras: Filter Print Post Top
InvisibleFooMan
 User Gallery

Folding@home Statistics
Registered: 02/02/05
Posts: 8,957
Loc: Earth Flag
Trusted Cultivator
Re: What is LC used for? [Re: Ajm] * 1
    #14510321 - 05/25/11 03:10 PM (12 years, 8 months ago)

Quote:

Ajm said:
From your experience how long does it take to see visible growth, with an MS inoculation, in LC?




I hope that questions wasn't directed to RR. If it was :facepalm: :lol:

If not, germination times are about the same as when you inoculate cakes assuming the inoculant is viable and the LC was made properly


--------------------

Quick WBS Prep


Extras: Filter Print Post Top
OfflineAjm
Stranger
Male User Gallery

Registered: 08/03/10
Posts: 81
Last seen: 8 years, 10 months
Re: What is LC used for? [Re: FooMan] * 1
    #14510394 - 05/25/11 03:28 PM (12 years, 8 months ago)

Quote:

FooMan said:
Quote:

Ajm said:
From your experience how long does it take to see visible growth, with an MS inoculation, in LC?




I hope that questions wasn't directed to RR. If it was :facepalm: :lol:

If not, germination times are about the same as when you inoculate cakes assuming the inoculant is viable and the LC was made properly




:laugh2: It does appear like that. I just meant in general. RR's probably like  :stonedjerk: get outta here dude. Anyways thanks for the reply bro.


--------------------
" Life is just a ride " --Bill Hicks


Extras: Filter Print Post Top
Invisibleslapphappypill
Enthusiast!
 User Gallery


Folding@home Statistics
Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
Re: What is LC used for? [Re: RogerRabbit] * 1
    #14510533 - 05/25/11 03:52 PM (12 years, 8 months ago)

You can roll your eyes when I say that, but I've never had a contaminated LC. I've seen no growth, but never contams *knock on wood*.

As far as LC goes, it does take longer initially, however once you have a good LC going, you can extend it real far and fly through inoculating qt jars without G2g. I flame sterilize my needle once before getting the LC, once after, then I only use alcohol in between each jar.

And honestly not a single contam ever. Not one 1/2 pint, pint, or QT jar has seen contam'd LC.

Only spore syringes have caused me problems so I start spores on agar now, then take the agar chunk to LC


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


Extras: Filter Print Post Top
Invisibleslapphappypill
Enthusiast!
 User Gallery


Folding@home Statistics
Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
Re: What is LC used for? [Re: FooMan] * 1
    #14510548 - 05/25/11 03:54 PM (12 years, 8 months ago)

Quote:

FooMan said:
Quote:

Ajm said:
From your experience how long does it take to see visible growth, with an MS inoculation, in LC?




I hope that questions wasn't directed to RR. If it was :facepalm: :lol:

If not, germination times are about the same as when you inoculate cakes assuming the inoculant is viable and the LC was made properly




Yeah, figure about 5-10 days before you can see growth in the LC, and 2 weeks before the LC is ready. I'll test my LC at 2 weeks, but let it sit for another week or so before I put into the fridge


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


Extras: Filter Print Post Top
OfflineAjm
Stranger
Male User Gallery

Registered: 08/03/10
Posts: 81
Last seen: 8 years, 10 months
Re: What is LC used for? [Re: slapphappypill] * 1
    #14510661 - 05/25/11 04:16 PM (12 years, 8 months ago)

Alright cool, thanks man  :peace:


--------------------
" Life is just a ride " --Bill Hicks


Extras: Filter Print Post Top
InvisibleShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~
Male User Gallery


Folding@home Statistics
Registered: 07/18/10
Posts: 8,139
Loc: EVERY WHERE
Re: What is LC used for? [Re: total]
    #14512213 - 05/25/11 09:49 PM (12 years, 8 months ago)

Quote:

total said:
I have to disagree with RR as well... :shrug:
Just because YOU dont use that method, doesnt mean it doesnt work...

I've been doing this for 10 years, so i consider myself an experienced grower...And have used LC's to extend my last bit of spores...

And as a matter of fact, i used to use agar's baby bottle tek along time ago...
You calling my man agar a n00b RR? :oldman:

(You say that no experienced grower would use a LC due to a high contam risk?  I say they arent an experienced grower if they cant grow a clean LC :shrug:)



:imwithstupid:
i just made 4 LC's and there all tested and are good:shrug:
:2cents:


--------------------
AMU=Amateur Mycologists United

AMU ------->:opensign:--->:goatse:<---:opensign:<------Q&A
:squirrelnut:I'm just a squirrel man, trying to get a nut:urinate:

:v::v:

:opensign: VVV Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV :opensign:


Extras: Filter Print Post Top
InvisibleDoctor_Inoc
Vintage Hand
Registered: 04/30/11
Posts: 646
Loc: Flag
Re: What is LC used for? [Re: ShadOWCrack]
    #14512369 - 05/25/11 10:18 PM (12 years, 8 months ago)

Quote:

As far as LC goes, it does take longer initially, however once you have a good LC going, you can extend it real far and fly through inoculating qt jars without G2g. I flame sterilize my needle once before getting the LC, once after, then I only use alcohol in between each jar.



My biggest issues with LC's are that you can't be 100% sure your LC's clean until test substrates have been inoculated.  The other issue is the extra time, steps, and materials needed to make the medium then test it for viability.

I would have transfered that agar culture to quart sized grain jars instead of the LC.  At the time it takes to grow out the LC then test it for viability, you could have 10 times the amount of fully colonized grain jars that the amount of culture on agar you begin with would have allowed you to inoculate before or by the time your LC's have been tested for viability.


Extras: Filter Print Post Top
Invisibleslapphappypill
Enthusiast!
 User Gallery


Folding@home Statistics
Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
Re: What is LC used for? [Re: Doctor_Inoc]
    #14512396 - 05/25/11 10:23 PM (12 years, 8 months ago)

Quote:

Doctor_Inoc said:
Quote:

As far as LC goes, it does take longer initially, however once you have a good LC going, you can extend it real far and fly through inoculating qt jars without G2g. I flame sterilize my needle once before getting the LC, once after, then I only use alcohol in between each jar.



My biggest issues with LC's are that you can't be 100% sure your LC's clean until test substrates have been inoculated.  The other issue is the extra time, steps, and materials needed to make the medium then test it for viability.

I would have transfered that agar culture to quart sized grain jars instead of the LC.  At the time it takes to grow out the LC then test it for viability, you could have 10 times the amount of fully colonized grain jars that the amount of culture on agar you begin with would have allowed you to inoculate before or by the time your LC's have been tested for viability.





True, but my LC lets me use the same myc for a few months. I can split it up to 50 jars one week, 50 jars 2 weeks later, and so on without having to go through my GB or sterilizing my Bathroom as much. LC syringes work great for me right now! as far as commercial growing is concerned, I will eventually start G2G for all my edibles as I'll be doing hundreds of jars at a time and LC wouldn't make sense for that. Spawn bags are also more in m future for spawn prep to inoculate straw logs as well as shiitake beds


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


Extras: Filter Print Post Top
InvisibleShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~
Male User Gallery


Folding@home Statistics
Registered: 07/18/10
Posts: 8,139
Loc: EVERY WHERE
Re: What is LC used for? [Re: Doctor_Inoc]
    #14512435 - 05/25/11 10:29 PM (12 years, 8 months ago)

Quote:

Doctor_Inoc said:
Quote:

As far as LC goes, it does take longer initially, however once you have a good LC going, you can extend it real far and fly through inoculating qt jars without G2g. I flame sterilize my needle once before getting the LC, once after, then I only use alcohol in between each jar.



My biggest issues with LC's are that you can't be 100% sure your LC's clean until test substrates have been inoculated.  The other issue is the extra time, steps, and materials needed to make the medium then test it for viability.

I would have transfered that agar culture to quart sized grain jars instead of the LC.  At the time it takes to grow out the LC then test it for viability, you could have 10 times the amount of fully colonized grain jars that the amount of culture on agar you begin with would have allowed you to inoculate before or by the time your LC's have been tested for viability.



its not hard for me because i have all the stuff to make the LC,AGAR. i test my lc's on agar and you  can tell in just a few days:shrug:


--------------------
AMU=Amateur Mycologists United

AMU ------->:opensign:--->:goatse:<---:opensign:<------Q&A
:squirrelnut:I'm just a squirrel man, trying to get a nut:urinate:

:v::v:

:opensign: VVV Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV :opensign:


Extras: Filter Print Post Top
Offlinear1es
Psychonaut
Male User Gallery


Registered: 07/09/10
Posts: 776
Last seen: 1 year, 3 months
Re: What is LC used for? [Re: ShadOWCrack]
    #14512633 - 05/25/11 11:02 PM (12 years, 8 months ago)

in my experience LC is used to grow contams


--------------------
":mushroom2:here is nothing lost or wasted in this life.” - Bhagavad Gita


Extras: Filter Print Post Top
InvisibleShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~
Male User Gallery


Folding@home Statistics
Registered: 07/18/10
Posts: 8,139
Loc: EVERY WHERE
Re: What is LC used for? [Re: ar1es]
    #14512927 - 05/25/11 11:47 PM (12 years, 8 months ago)

Quote:

ar1es said:
in my experience LC is used to grow contams



and in mine, i get mushies from my LC



@ OP
feel free to :pm: me if u have any more ?'s


--------------------
AMU=Amateur Mycologists United

AMU ------->:opensign:--->:goatse:<---:opensign:<------Q&A
:squirrelnut:I'm just a squirrel man, trying to get a nut:urinate:

:v::v:

:opensign: VVV Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV :opensign:


Extras: Filter Print Post Top
Invisibleslapphappypill
Enthusiast!
 User Gallery


Folding@home Statistics
Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
Re: What is LC used for? [Re: ShadOWCrack]
    #14512944 - 05/25/11 11:50 PM (12 years, 8 months ago)

Quote:

ShadOWCrack said:
Quote:

Doctor_Inoc said:
Quote:

As far as LC goes, it does take longer initially, however once you have a good LC going, you can extend it real far and fly through inoculating qt jars without G2g. I flame sterilize my needle once before getting the LC, once after, then I only use alcohol in between each jar.



My biggest issues with LC's are that you can't be 100% sure your LC's clean until test substrates have been inoculated.  The other issue is the extra time, steps, and materials needed to make the medium then test it for viability.

I would have transfered that agar culture to quart sized grain jars instead of the LC.  At the time it takes to grow out the LC then test it for viability, you could have 10 times the amount of fully colonized grain jars that the amount of culture on agar you begin with would have allowed you to inoculate before or by the time your LC's have been tested for viability.



its not hard for me because i have all the stuff to make the LC,AGAR. i test my lc's on agar and you  can tell in just a few days:shrug:




I have all the stuff also, just not worth the hassle for me until I get a flow hood (coming soon in a few months as I'm starting a farm and working on filing for my government grant)


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


Extras: Filter Print Post Top
InvisibleShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~
Male User Gallery


Folding@home Statistics
Registered: 07/18/10
Posts: 8,139
Loc: EVERY WHERE
Re: What is LC used for? [Re: slapphappypill]
    #14512985 - 05/25/11 11:58 PM (12 years, 8 months ago)

:datass:


--------------------
AMU=Amateur Mycologists United

AMU ------->:opensign:--->:goatse:<---:opensign:<------Q&A
:squirrelnut:I'm just a squirrel man, trying to get a nut:urinate:

:v::v:

:opensign: VVV Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV :opensign:


Extras: Filter Print Post Top
Invisiblethor_ak
Mr.
Male


Registered: 04/03/02
Posts: 479
Loc: USA Flag
Re: What is LC used for? [Re: runepanther]
    #14513089 - 05/26/11 12:29 AM (12 years, 8 months ago)

Quote:

Gigabyt3r said:
I followed the tutorial and it all looks fine and dandy, but what are the mycelium for? Does it speed up the process? If so, how? How are the used in regards to the PF tek? I don't really understand what you do with it then?

Wow thats alot of questions! Thanks in advance




Quote:

runepanther said:
Myc is what would grow if you just dropped spores in the jars...





Mycelium is the white stuff in the jars, it consumes your brown rice

flour/vermiculite, and takes nutrients from it to produce mushrooms.

Mycelium comes from spores, once they are moisturized and introduced

into a habitable habitat. Which usually takes between 3-10 days once

you shoot them into your jars VIA syringe. But, if you make a liquid

culture, the mycelium is already growing/living, so when you take that,

and shoot that into your jars VIA syringe, growth will start

immediately. It just speeds up time. If this is your first grow, I

would say just go with the standard syringe/spore tek. Keep it simple

until you understand what you are working with. IMO. Good luck.


--------------------
"A smart man learns from his mistakes. A wise man, learns from the mistakes of others. A woman, makes you pay for these mistakes."


Extras: Filter Print Post Top
Offlinear1es
Psychonaut
Male User Gallery


Registered: 07/09/10
Posts: 776
Last seen: 1 year, 3 months
Re: What is LC used for? [Re: thor_ak]
    #14515476 - 05/26/11 01:55 PM (12 years, 8 months ago)

i see the benefits of LC if you can get it to work


--------------------
":mushroom2:here is nothing lost or wasted in this life.” - Bhagavad Gita


Extras: Filter Print Post Top
Offlinear1es
Psychonaut
Male User Gallery


Registered: 07/09/10
Posts: 776
Last seen: 1 year, 3 months
Re: What is LC used for? [Re: ar1es]
    #14515516 - 05/26/11 02:03 PM (12 years, 8 months ago)

anybody ever take a look at this study ?

Developments in the production of mushroom mycelium in submerged liquid culture

Abstract

Yields are high and no special production problems are encountered when mushroom mycelia are grown in submerged liquid culture on simple carbohydrate and nitrogen compounds with mineral salts. Acceptable flavours are obtained with Agaricus campestris, Morchella crassipes, Lepiota rachodes and Coprinus comatus, but these flavours are less pronounced from liquid culture than from solid media.-Univ. Fla, Gainesville.


--------------------
":mushroom2:here is nothing lost or wasted in this life.” - Bhagavad Gita


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 4 days
Trusted Cultivator
OG Cultivator
Re: What is LC used for? [Re: ar1es]
    #14515994 - 05/26/11 03:35 PM (12 years, 8 months ago)

Yields of what are high?  They're not growing mushrooms on LC.  It's also about ten percent the speed of grains.  There's a reason it's rarely used.  I suppose a farm that's growing shiploads of mushrooms might have a use for it, but a small mushroom farm like mine that only turns out a few thousand pounds per month sure as heck doesn't benefit from LC.  It's too slow.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
Offlinedrwatson
Slacker
 User Gallery


Registered: 10/29/07
Posts: 686
Loc: nowhere land
Last seen: 11 years, 2 months
Re: What is LC used for? [Re: RogerRabbit]
    #14516116 - 05/26/11 03:58 PM (12 years, 8 months ago)

I tried LC for awhile and it worked for me.  But when I stepped up to G2G transfers I never looked back.  When time is a factor why  leave any  room for second guessing?  You know 100% if a grain jar is good or not immediately upon opening it. 

By the time you've made 10 more jars from good spawn with  G2G transfer the LC test jars will just be coming back positive or negative (if they're bad you start over). 

By the time the viability of LC is confirmed and jars are colonized G2G will have been mixed with a substrate and be well on it's way to giving fruits. 

LC might have a place for cloning a specimen maybe. . . but you could just as easily do this with a grain jar too and know 100% that your cloned spawn is viable or not, without the need to make a second test jar.


Extras: Filter Print Post Top
Offlinedrwatson
Slacker
 User Gallery


Registered: 10/29/07
Posts: 686
Loc: nowhere land
Last seen: 11 years, 2 months
Re: What is LC used for? [Re: drwatson]
    #14516144 - 05/26/11 04:02 PM (12 years, 8 months ago)

Oh and to the OP, LC is primarily used to extent the amount of inoculate you have, i.e. Spores from a syringe.  A Grain-to-Grain transfer (G2G) is using spawn from a jar you know is good to make more jars thus extending the amount of inoculate you have.


Extras: Filter Print Post Top
Offlinerealcarlos
Stranger
Registered: 05/04/10
Posts: 292
Last seen: 2 years, 5 months
Re: What is LC used for? [Re: drwatson]
    #14516240 - 05/26/11 04:23 PM (12 years, 8 months ago)

What if you use Agar's grain lc tek? Wouldn't you know if its contam or not. That way there is no reason to test it?
any thoughts?


Extras: Filter Print Post Top
InvisibleSpector
Just Disappeared

Registered: 05/13/11
Posts: 167
Loc: A Scientific Location....
Re: What is LC used for? [Re: drwatson]
    #14516300 - 05/26/11 04:37 PM (12 years, 8 months ago)

to op, What im getting from this is it really matters what stage your at in cultivating mushrooms.
yes you could do G2Gs, but that would require a Hood and great technique, Becuase your dealing with sterile grains and cant afford to be wastin every other jar. with Sum of the "poor mans LC teks" all you require is a clean syringe and A hole on your jars, + a glove box.

Its also a great way to expand your Spores so you dont have to keep buying syringes over and over again. yes you could do it this with agar, but again you need to have sum advance items and techniques that most ppl wouldnt have that just cultivate for fun.


Edited by Spector (05/27/11 10:43 AM)


Extras: Filter Print Post Top
OfflinePerun
Mahapralaya...
Male User Gallery


Registered: 11/11/10
Posts: 585
Last seen: 2 years, 2 months
Re: What is LC used for? [Re: Spector]
    #14519331 - 05/27/11 04:57 AM (12 years, 8 months ago)

Quote:

Spector said:
What im getting from this is it really matters what stage your at in cultivating mushrooms.
yes you could do G2Gs, but that would require a Hood and great technique, Becuase your dealing with sterile grains and cant afford to be wastin every other jar. with Sum of the "poor mans LC teks" all you require is a clean syringe and A hole on your jars, + a glove box.

Its also a great way to expand your Spores so you dont have to keep buying syringes over and over again. yes you could do it this with agar, but again you need to have sum advance items and techniques that most ppl wouldnt have that just cultivate for fun.




G2G does not require a hood! I had been doing g2g in open air for a long time with no contam at all and only started using a glove box for it when i started working with agar,cuz before agar work all my work was done in open air with no contam issues what so ever! All you need is cleen small room,cleen clothes,gloves,mask,lot of air and surface sanitizer and that is it!

:2cents:


--------------------
First:                                                        Then:


Extras: Filter Print Post Top
Offlinerealcarlos
Stranger
Registered: 05/04/10
Posts: 292
Last seen: 2 years, 5 months
Re: What is LC used for? [Re: Perun]
    #14519989 - 05/27/11 09:37 AM (12 years, 8 months ago)

perun what about grain lcs are these as risky as other lcs?


Extras: Filter Print Post Top
InvisibleFractalXplora
Grainiack
Male


Folding@home Statistics
Registered: 02/11/06
Posts: 2,494
Loc: UK Flag
Trusted Cultivator
Re: What is LC used for? [Re: realcarlos]
    #14520050 - 05/27/11 09:57 AM (12 years, 8 months ago)

I like the quickness of a 60ml syringe of LC, you can rip through and knock up 12 or more spawn jars with that once you know its a goodun!

I just wanted to make a home made stir plate too :wink:

But agar wedge too grain is 100% more effective with nearly zero contam rate if your plates are good.

I enjoy both methods.

Also agar wedge to LC is cool as hell and getting it on the stir plate rocks. Nice way to start a Liquid culture.:thumbup:


--------------------


Extras: Filter Print Post Top
Offlinetotal
Post Office Tyvek Advocator
Male User Gallery


Folding@home Statistics
Registered: 10/03/04
Posts: 11,405
Last seen: 6 months, 24 days
Trusted Cultivator
Re: What is LC used for? [Re: Perun]
    #14520103 - 05/27/11 10:21 AM (12 years, 8 months ago)

Quote:

Perun said:
G2G does not require a hood! I had been doing g2g in open air for a long time with no contam at all
:2cents:




:facepalm:
This is horrible info...:nonono:
Atleast cut 2 holes in a rubbermaid and flip it upside down on a table and use a still air box...

Its no point in risking time/money/energy on a chance that it "might" work...

Take it from a >10yr grower, that is not the way to do things...
A $10 still air box is one of the best investments you can make asides from a good pc...


Extras: Filter Print Post Top
InvisibleSpector
Just Disappeared

Registered: 05/13/11
Posts: 167
Loc: A Scientific Location....
Re: What is LC used for? [Re: realcarlos]
    #14520248 - 05/27/11 11:01 AM (12 years, 8 months ago)

Quote:

realcarlos said:
perun what about grain lcs are these as risky as other lcs?




lol carlos, you've been asking and every ones been ignoring you. well i would say with Grain LC's, deponding on the person they are still AS risky as Honey LC's. Yes you confirm in the begining that it is in fact Mycelium. but during the process of moving water To the grain jar, then moving it back to the sterile water jar there are a ton of things that could go wrong with the water and beginners.
tho, it is faster and sum what safer then Sugar Lc, were you have to wait then noc up a Jar to make sure its myc.


Extras: Filter Print Post Top
Offlinerealcarlos
Stranger
Registered: 05/04/10
Posts: 292
Last seen: 2 years, 5 months
Re: What is LC used for? [Re: Spector]
    #14520265 - 05/27/11 11:08 AM (12 years, 8 months ago)

lol
thanks a million spector


Extras: Filter Print Post Top
InvisibleLing Ling
Tree Climber
 User Gallery

Registered: 10/07/15
Posts: 162
Loc: The Twilight Zone Flag
Re: What is LC used for? [Re: Perun]
    #22537358 - 11/17/15 12:31 PM (8 years, 2 months ago)

Quote:

Perun said:
Mycelium can be cultured in a liquid (as opposed to a solid like vermiculite/brown rice flour) which you can then use to easily inoculate substrate and start mycelium growth much faster than if you were using spores.

But...LC is a "two bladed sword" you dont know what are you growing untill you test it!

Try this...its much better then any sugar based LC!

http://www.shroomery.org/forums/showflat.php?Cat=&Board=30&Number=6817701&fpart=&PHPSESSID=




To view your link I need to be a member for 14 days and have ten posts. I'm only at nine. So this will make it ten.... Sorry for the bump!


--------------------
If any fellow Shroomers have watched the YouTube video "a planned trespass against the mind of man" and are going to pursue your right to live as a man... Or live in Canada under the legal sense of a man (freeman equivalent in the U.S)
Please pm me!!




Normality is a product of perception!


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: What is LC used for? [Re: Ling Ling]
    #22537737 - 11/17/15 01:51 PM (8 years, 2 months ago)

This thread made me grind my teeth :frown:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3  [ show all ]

Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Kratom Powder for Sale   North Spore Bulk Substrate   Bridgetown Botanicals CBD Concentrates   Left Coast Kratom Buy Kratom Capsules   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Mex-A LC --- PICS
( 1 2 all )
themycologist 4,812 38 04/16/10 10:06 AM
by KillaFoRilla
* my LC stalled rDr4g0n 2,467 15 04/13/06 12:20 PM
by JSshroom
* Far into the LC Myc Matrix
( 1 2 3 all )
agar 9,458 44 05/26/05 01:36 PM
by Aeolus1369
* Milky LC = contam? BMArts 2,231 6 04/10/06 09:09 AM
by blackout
* LC, check it primordialoddity 464 1 11/06/07 02:25 PM
by primordialoddity
* BB LC Tek: Regarding the bottles you buy.
( 1 2 all )
Delinquentes 2,014 20 03/01/06 02:46 AM
by onetime
* LC isnt showing signs of growth vintage_gonzo 1,105 12 04/08/06 05:59 PM
by BMArts
* Probable Nightmare...please confirm Cubenisseur 835 12 04/14/06 06:50 AM
by Cubenisseur

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
3,804 topic views. 21 members, 172 guests and 36 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.039 seconds spending 0.012 seconds on 14 queries.