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ShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~




Registered: 07/18/10
Posts: 8,139
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Re: What is LC used for? [Re: total]
#14512213 - 05/25/11 09:49 PM (12 years, 8 months ago) |
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Quote:
total said: I have to disagree with RR as well...  Just because YOU dont use that method, doesnt mean it doesnt work...
I've been doing this for 10 years, so i consider myself an experienced grower...And have used LC's to extend my last bit of spores...
And as a matter of fact, i used to use agar's baby bottle tek along time ago... You calling my man agar a n00b RR? 
(You say that no experienced grower would use a LC due to a high contam risk? I say they arent an experienced grower if they cant grow a clean LC )
 i just made 4 LC's and there all tested and are good
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Doctor_Inoc
Vintage Hand
Registered: 04/30/11
Posts: 646
Loc:
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Quote:
As far as LC goes, it does take longer initially, however once you have a good LC going, you can extend it real far and fly through inoculating qt jars without G2g. I flame sterilize my needle once before getting the LC, once after, then I only use alcohol in between each jar.
My biggest issues with LC's are that you can't be 100% sure your LC's clean until test substrates have been inoculated. The other issue is the extra time, steps, and materials needed to make the medium then test it for viability.
I would have transfered that agar culture to quart sized grain jars instead of the LC. At the time it takes to grow out the LC then test it for viability, you could have 10 times the amount of fully colonized grain jars that the amount of culture on agar you begin with would have allowed you to inoculate before or by the time your LC's have been tested for viability.
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Quote:
Doctor_Inoc said:
Quote:
As far as LC goes, it does take longer initially, however once you have a good LC going, you can extend it real far and fly through inoculating qt jars without G2g. I flame sterilize my needle once before getting the LC, once after, then I only use alcohol in between each jar.
My biggest issues with LC's are that you can't be 100% sure your LC's clean until test substrates have been inoculated. The other issue is the extra time, steps, and materials needed to make the medium then test it for viability.
I would have transfered that agar culture to quart sized grain jars instead of the LC. At the time it takes to grow out the LC then test it for viability, you could have 10 times the amount of fully colonized grain jars that the amount of culture on agar you begin with would have allowed you to inoculate before or by the time your LC's have been tested for viability.
True, but my LC lets me use the same myc for a few months. I can split it up to 50 jars one week, 50 jars 2 weeks later, and so on without having to go through my GB or sterilizing my Bathroom as much. LC syringes work great for me right now! as far as commercial growing is concerned, I will eventually start G2G for all my edibles as I'll be doing hundreds of jars at a time and LC wouldn't make sense for that. Spawn bags are also more in m future for spawn prep to inoculate straw logs as well as shiitake beds
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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ShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~




Registered: 07/18/10
Posts: 8,139
Loc: EVERY WHERE
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Quote:
Doctor_Inoc said:
Quote:
As far as LC goes, it does take longer initially, however once you have a good LC going, you can extend it real far and fly through inoculating qt jars without G2g. I flame sterilize my needle once before getting the LC, once after, then I only use alcohol in between each jar.
My biggest issues with LC's are that you can't be 100% sure your LC's clean until test substrates have been inoculated. The other issue is the extra time, steps, and materials needed to make the medium then test it for viability.
I would have transfered that agar culture to quart sized grain jars instead of the LC. At the time it takes to grow out the LC then test it for viability, you could have 10 times the amount of fully colonized grain jars that the amount of culture on agar you begin with would have allowed you to inoculate before or by the time your LC's have been tested for viability.
its not hard for me because i have all the stuff to make the LC,AGAR. i test my lc's on agar and you can tell in just a few days
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ar1es
Psychonaut



Registered: 07/09/10
Posts: 776
Last seen: 1 year, 3 months
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in my experience LC is used to grow contams
-------------------- " here is nothing lost or wasted in this life.” - Bhagavad Gita
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ShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~




Registered: 07/18/10
Posts: 8,139
Loc: EVERY WHERE
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Re: What is LC used for? [Re: ar1es]
#14512927 - 05/25/11 11:47 PM (12 years, 8 months ago) |
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Quote:
ar1es said: in my experience LC is used to grow contams
and in mine, i get mushies from my LC
 
@ OP feel free to me if u have any more ?'s
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Quote:
ShadOWCrack said:
Quote:
Doctor_Inoc said:
Quote:
As far as LC goes, it does take longer initially, however once you have a good LC going, you can extend it real far and fly through inoculating qt jars without G2g. I flame sterilize my needle once before getting the LC, once after, then I only use alcohol in between each jar.
My biggest issues with LC's are that you can't be 100% sure your LC's clean until test substrates have been inoculated. The other issue is the extra time, steps, and materials needed to make the medium then test it for viability.
I would have transfered that agar culture to quart sized grain jars instead of the LC. At the time it takes to grow out the LC then test it for viability, you could have 10 times the amount of fully colonized grain jars that the amount of culture on agar you begin with would have allowed you to inoculate before or by the time your LC's have been tested for viability.
its not hard for me because i have all the stuff to make the LC,AGAR. i test my lc's on agar and you can tell in just a few days
I have all the stuff also, just not worth the hassle for me until I get a flow hood (coming soon in a few months as I'm starting a farm and working on filing for my government grant)
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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ShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~




Registered: 07/18/10
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thor_ak
Mr.



Registered: 04/03/02
Posts: 479
Loc: USA
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Quote:
Gigabyt3r said: I followed the tutorial and it all looks fine and dandy, but what are the mycelium for? Does it speed up the process? If so, how? How are the used in regards to the PF tek? I don't really understand what you do with it then?
Wow thats alot of questions! Thanks in advance
Quote:
runepanther said: Myc is what would grow if you just dropped spores in the jars...
Mycelium is the white stuff in the jars, it consumes your brown rice
flour/vermiculite, and takes nutrients from it to produce mushrooms.
Mycelium comes from spores, once they are moisturized and introduced
into a habitable habitat. Which usually takes between 3-10 days once
you shoot them into your jars VIA syringe. But, if you make a liquid
culture, the mycelium is already growing/living, so when you take that,
and shoot that into your jars VIA syringe, growth will start
immediately. It just speeds up time. If this is your first grow, I
would say just go with the standard syringe/spore tek. Keep it simple
until you understand what you are working with. IMO. Good luck.
-------------------- "A smart man learns from his mistakes. A wise man, learns from the mistakes of others. A woman, makes you pay for these mistakes."
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ar1es
Psychonaut



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Re: What is LC used for? [Re: thor_ak]
#14515476 - 05/26/11 01:55 PM (12 years, 8 months ago) |
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i see the benefits of LC if you can get it to work
-------------------- " here is nothing lost or wasted in this life.” - Bhagavad Gita
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ar1es
Psychonaut



Registered: 07/09/10
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Re: What is LC used for? [Re: ar1es]
#14515516 - 05/26/11 02:03 PM (12 years, 8 months ago) |
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anybody ever take a look at this study ?
Developments in the production of mushroom mycelium in submerged liquid culture
Abstract
Yields are high and no special production problems are encountered when mushroom mycelia are grown in submerged liquid culture on simple carbohydrate and nitrogen compounds with mineral salts. Acceptable flavours are obtained with Agaricus campestris, Morchella crassipes, Lepiota rachodes and Coprinus comatus, but these flavours are less pronounced from liquid culture than from solid media.-Univ. Fla, Gainesville.
-------------------- " here is nothing lost or wasted in this life.” - Bhagavad Gita
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 4 days
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Re: What is LC used for? [Re: ar1es]
#14515994 - 05/26/11 03:35 PM (12 years, 8 months ago) |
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Yields of what are high? They're not growing mushrooms on LC. It's also about ten percent the speed of grains. There's a reason it's rarely used. I suppose a farm that's growing shiploads of mushrooms might have a use for it, but a small mushroom farm like mine that only turns out a few thousand pounds per month sure as heck doesn't benefit from LC. It's too slow. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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drwatson
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Registered: 10/29/07
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I tried LC for awhile and it worked for me. But when I stepped up to G2G transfers I never looked back. When time is a factor why leave any room for second guessing? You know 100% if a grain jar is good or not immediately upon opening it.
By the time you've made 10 more jars from good spawn with G2G transfer the LC test jars will just be coming back positive or negative (if they're bad you start over).
By the time the viability of LC is confirmed and jars are colonized G2G will have been mixed with a substrate and be well on it's way to giving fruits.
LC might have a place for cloning a specimen maybe. . . but you could just as easily do this with a grain jar too and know 100% that your cloned spawn is viable or not, without the need to make a second test jar.
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drwatson
Slacker



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Re: What is LC used for? [Re: drwatson]
#14516144 - 05/26/11 04:02 PM (12 years, 8 months ago) |
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Oh and to the OP, LC is primarily used to extent the amount of inoculate you have, i.e. Spores from a syringe. A Grain-to-Grain transfer (G2G) is using spawn from a jar you know is good to make more jars thus extending the amount of inoculate you have.
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realcarlos
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Registered: 05/04/10
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Re: What is LC used for? [Re: drwatson]
#14516240 - 05/26/11 04:23 PM (12 years, 8 months ago) |
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What if you use Agar's grain lc tek? Wouldn't you know if its contam or not. That way there is no reason to test it? any thoughts?
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Spector
Just Disappeared

Registered: 05/13/11
Posts: 167
Loc: A Scientific Location....
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Re: What is LC used for? [Re: drwatson]
#14516300 - 05/26/11 04:37 PM (12 years, 8 months ago) |
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to op, What im getting from this is it really matters what stage your at in cultivating mushrooms. yes you could do G2Gs, but that would require a Hood and great technique, Becuase your dealing with sterile grains and cant afford to be wastin every other jar. with Sum of the "poor mans LC teks" all you require is a clean syringe and A hole on your jars, + a glove box.
Its also a great way to expand your Spores so you dont have to keep buying syringes over and over again. yes you could do it this with agar, but again you need to have sum advance items and techniques that most ppl wouldnt have that just cultivate for fun.
Edited by Spector (05/27/11 10:43 AM)
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Perun
Mahapralaya...



Registered: 11/11/10
Posts: 585
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Re: What is LC used for? [Re: Spector]
#14519331 - 05/27/11 04:57 AM (12 years, 8 months ago) |
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Quote:
Spector said: What im getting from this is it really matters what stage your at in cultivating mushrooms. yes you could do G2Gs, but that would require a Hood and great technique, Becuase your dealing with sterile grains and cant afford to be wastin every other jar. with Sum of the "poor mans LC teks" all you require is a clean syringe and A hole on your jars, + a glove box.
Its also a great way to expand your Spores so you dont have to keep buying syringes over and over again. yes you could do it this with agar, but again you need to have sum advance items and techniques that most ppl wouldnt have that just cultivate for fun.
G2G does not require a hood! I had been doing g2g in open air for a long time with no contam at all and only started using a glove box for it when i started working with agar,cuz before agar work all my work was done in open air with no contam issues what so ever! All you need is cleen small room,cleen clothes,gloves,mask,lot of air and surface sanitizer and that is it!
-------------------- First: Then:
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realcarlos
Stranger
Registered: 05/04/10
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Re: What is LC used for? [Re: Perun]
#14519989 - 05/27/11 09:37 AM (12 years, 8 months ago) |
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perun what about grain lcs are these as risky as other lcs?
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FractalXplora
Grainiack




Registered: 02/11/06
Posts: 2,494
Loc: UK
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Re: What is LC used for? [Re: realcarlos]
#14520050 - 05/27/11 09:57 AM (12 years, 8 months ago) |
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I like the quickness of a 60ml syringe of LC, you can rip through and knock up 12 or more spawn jars with that once you know its a goodun!
I just wanted to make a home made stir plate too 
But agar wedge too grain is 100% more effective with nearly zero contam rate if your plates are good.
I enjoy both methods.
Also agar wedge to LC is cool as hell and getting it on the stir plate rocks. Nice way to start a Liquid culture.
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total
Post Office Tyvek Advocator




Registered: 10/03/04
Posts: 11,405
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Re: What is LC used for? [Re: Perun]
#14520103 - 05/27/11 10:21 AM (12 years, 8 months ago) |
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Quote:
Perun said: G2G does not require a hood! I had been doing g2g in open air for a long time with no contam at all

 This is horrible info... Atleast cut 2 holes in a rubbermaid and flip it upside down on a table and use a still air box...
Its no point in risking time/money/energy on a chance that it "might" work...
Take it from a >10yr grower, that is not the way to do things... A $10 still air box is one of the best investments you can make asides from a good pc...
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