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Offlineglitche
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Registered: 04/07/11
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Spore solution to Agar
    #14508733 - 05/25/11 07:53 AM (12 years, 9 months ago)

Does it matter if I put spore solution on top of the agar without penetrating the Agar? Also how long does it take before signs of growth show on agar when using a spore solution?

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InvisibleShadOWCrack
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Re: Spore solution to Agar [Re: glitche]
    #14508773 - 05/25/11 08:07 AM (12 years, 9 months ago)

you can use spore :print::syringe: or tissue.

just get one drop in the center and you will be good.

it can take 3-7 days sometimes longer.


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InvisibleTranscendingLife
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Re: Spore solution to Agar [Re: ShadOWCrack]
    #14508823 - 05/25/11 08:27 AM (12 years, 9 months ago)

:imwithstupid:

less is more when using spores on agar. :2cents:


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Offlineglitche
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Re: Spore solution to Agar [Re: TranscendingLife]
    #14509535 - 05/25/11 12:02 PM (12 years, 9 months ago)

Damn I put more than a drop, oh well hopefully it will do something. It's only been a day so we will see :grin:

Thanks guys !

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Re: Spore solution to Agar [Re: glitche]
    #14511879 - 05/25/11 08:51 PM (12 years, 9 months ago)

:awecid:


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Offlineglitche
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Re: Spore solution to Agar [Re: ShadOWCrack]
    #14522266 - 05/27/11 05:19 PM (12 years, 9 months ago)

If anyone is interested I put 4 drops of spore solution in this agar
(which is specially made for fungi) without penetrating it and these are my results after 2 days

First one



I got sloppy with the second one



Edited by glitche (05/27/11 05:20 PM)

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Re: Spore solution to Agar [Re: glitche]
    #14522623 - 05/27/11 06:25 PM (12 years, 9 months ago)

why dident you use micro tape

your 3 vents are exposed to open air

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Offlineglitche
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Re: Spore solution to Agar [Re: jokefox]
    #14522639 - 05/27/11 06:28 PM (12 years, 9 months ago)

3 vents? I'm confused :confused:

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Invisiblejokefox
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Re: Spore solution to Agar [Re: glitche]
    #14522655 - 05/27/11 06:32 PM (12 years, 9 months ago)

look at the three slits on the lid , its called a vent,

you see the space between the lid and the bottom around where they meet,
air can get there,

go look how to wrap a dish properly on youtube or something
youtube has everything, its scary...

untell then leave the agar alone, so you dont waste any,
and a tip , when pouring agar in dishes use a syringe to squirt agar in

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OfflineCaptainAhab


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Re: Spore solution to Agar [Re: glitche]
    #14522657 - 05/27/11 06:33 PM (12 years, 9 months ago)

Quote:

glitche said:
If anyone is interested I put 4 drops of spore solution in this agar
(which is specially made for fungi) without penetrating it and these are my results after 2 days

First one



I got sloppy with the second one







that looks like bacteria or something you don't want. It's not mycelium.


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Edited by CaptainAhab (05/27/11 06:33 PM)

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Offlineglitche
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Re: Spore solution to Agar [Re: jokefox]
    #14522790 - 05/27/11 07:01 PM (12 years, 9 months ago)

Quote:

jokefox said:
look at the three slits on the lid , its called a vent,

you see the space between the lid and the bottom around where they meet,
air can get there,

go look how to wrap a dish properly on youtube or something
youtube has everything, its scary...

untell then leave the agar alone, so you dont waste any,
and a tip , when pouring agar in dishes use a syringe to squirt agar in






These don't have slits. The lab I work in deals with enzymatic disaggregation so we have special ones. As for that being mycelium or not I'm not sure. Also, I did use a syringe (I'm just a poor shot) how else would I have done it?

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InvisibleDoc_T
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Re: Spore solution to Agar [Re: glitche]
    #14522805 - 05/27/11 07:05 PM (12 years, 9 months ago)

Second one looks bacterial.


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InvisibleShadOWCrack
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Re: Spore solution to Agar [Re: glitche]
    #14522821 - 05/27/11 07:09 PM (12 years, 9 months ago)

iam not sure about your dishes. it could be bac.. give it a few more days and see what u get. did you make ur :syringe: or is it from a vender?

this is a 2nd transfer i did working on an iso.



goodluck


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Offlineglitche
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Registered: 04/07/11
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Re: Spore solution to Agar [Re: ShadOWCrack]
    #14522859 - 05/27/11 07:18 PM (12 years, 9 months ago)

thanks guys, The spore prints are from wild cubensis. I don't think it is bacteria because it's growing exactly where the solution hit the agar.Then again the spore solution may be contaminated. I guess ill find out sooner or later. :tongue:

Edited by glitche (05/27/11 07:19 PM)

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Re: Spore solution to Agar [Re: glitche]
    #14522918 - 05/27/11 07:30 PM (12 years, 9 months ago)

Contam.


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Invisiblejokefox
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Re: Spore solution to Agar [Re: Doc_T]
    #14522938 - 05/27/11 07:33 PM (12 years, 9 months ago)

you dont work in a lab haha

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Offlineglitche
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Re: Spore solution to Agar [Re: Doc_T]
    #14523053 - 05/27/11 07:52 PM (12 years, 9 months ago)

Quote:

Doc_T said:
Contam.




:thumbdown:

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InvisibleSpector
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Re: Spore solution to Agar [Re: glitche]
    #14523101 - 05/27/11 08:00 PM (12 years, 9 months ago)

your going to get far in this hobby lol
dont like to a admit when your wrong, when the ppl that are telling you are the ppl who have been doing this forever.


i like your sterile precautions when applying hairy electrical tape.

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OfflineWing
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Re: Spore solution to Agar [Re: Spector]
    #14523131 - 05/27/11 08:06 PM (12 years, 9 months ago)

Your going to have contams printing wild cubes. No way around it bro.


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Offlineglitche
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Re: Spore solution to Agar [Re: Wing]
    #14526561 - 05/28/11 01:58 PM (12 years, 9 months ago)

Quote:

winged_1 said:
Your going to have contams printing wild cubes. No way around it bro.




well someone must have figured out how to do it because every cube use to be wild right?

Are you saying that every time I take a spore print from a wild cube and inoculate agar or substrate they will become contaminated or there is just a high probability it will?

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