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OfflineRogueTrippeR
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Re: Gettin' My Learn On! [Re: HorizonSpawn]
    #14503366 - 05/24/11 08:37 AM (12 years, 9 months ago)

Quote:

HorizonSpawn said:
How else would I prepare contaminate-free agar plates and spore print transfers?




By using a SAB (still-air-box, glove box)

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InvisibleHorizonSpawn
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Re: Gettin' My Learn On! [Re: RogueTrippeR]
    #14503739 - 05/24/11 10:50 AM (12 years, 9 months ago)

Quote:

RogueTrippeR said:
Quote:

HorizonSpawn said:
How else would I prepare contaminate-free agar plates and spore print transfers?




By using a SAB (still-air-box, glove box)




Was hoping to bypass that build. However; if necessary, ill try my hand at making spore prints within one... when the time comes (hopefully).

:smile:


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Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:

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Offlinesteelmonkey
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Re: Gettin' My Learn On! [Re: HorizonSpawn]
    #14503765 - 05/24/11 10:57 AM (12 years, 9 months ago)

Its possible to make prints without one but I wouldn't touch agar without it


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OfflineRogueTrippeR
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Re: Gettin' My Learn On! [Re: HorizonSpawn]
    #14503810 - 05/24/11 11:15 AM (12 years, 9 months ago)

Quote:

HorizonSpawn said:
Quote:

RogueTrippeR said:
Quote:

HorizonSpawn said:
How else would I prepare contaminate-free agar plates and spore print transfers?




By using a SAB (still-air-box, glove box)




Was hoping to bypass that build. However; if necessary, ill try my hand at making spore prints within one... when the time comes (hopefully).

:smile:



Making a SAB should be pretty easy to do.  Then you can stretch your mycelium a 100 fold.


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Cluster Headache

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InvisibleHorizonSpawn
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Re: Gettin' My Learn On! [Re: RogueTrippeR]
    #14503872 - 05/24/11 11:26 AM (12 years, 9 months ago)

Working with agar is way down the line, in my new Mycology adventure. Probably not until after I've built a clean-room with a flowhood...  However; I definitely will need to make spore prints and then make up some spore syringes, long before that point. If I can do both in a cheap-ass, tossed together, still air box... Then I'm definitely going to reread those TEKs :smile:  Eh, I'll reread them, regardless! :laugh:


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Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:

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Invisiblezydisqwap
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Re: Gettin' My Learn On! [Re: HorizonSpawn]
    #14503955 - 05/24/11 11:42 AM (12 years, 9 months ago)

yup.

you can pour agar and take prints in a glovebox as long as you follow a sterile and quick procedure.  it also helps to go through the motions a few times to get the hang of it so that when it comes time to pour agar, it will be much easier.

as long as no air can get into the box, and you have sterilized the inside with rubbing alcohol and some spray disinfectant (and given time for the spray to settle, about 15-20 minutes), you will be good.

get the hang of a glovebox down before thinking about a flowhood.  they cost all of $10 to build and will allow you to perfect a sterile procedure that a flowhood is a useless without.


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****Cheap Plastic Containers in Bulk****

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Offlinesteelmonkey
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Re: Gettin' My Learn On! [Re: zydisqwap]
    #14504367 - 05/24/11 12:54 PM (12 years, 9 months ago)

Very good advice Zyd well said


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InvisibleHorizonSpawn
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Re: Gettin' My Learn On! [Re: steelmonkey]
    #14505787 - 05/24/11 05:54 PM (12 years, 9 months ago)

It has been more than eight hours, since I inoculated my "pf cakes"... But I still don't have any mature mushrooms yet!!!!!
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He he he... This next month or two is point to be brutal, LOLz.


--------------------
Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:

Extras: Filter Print Post Top
InvisibleHorizonSpawn
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Re: Gettin' My Learn On! [Re: HorizonSpawn]
    #14511974 - 05/25/11 09:06 PM (12 years, 9 months ago)

So far, my temps seem to be staying between 71~75degF. Now I just need to not mess with them for a week, lol.


--------------------
Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:

Extras: Filter Print Post Top
Offlinesteelmonkey
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Re: Gettin' My Learn On! [Re: HorizonSpawn]
    #14512001 - 05/25/11 09:12 PM (12 years, 9 months ago)

The trick is to start more jars so your mind won't be on them:smirk:


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OfflineFrequentFryer
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Re: Gettin' My Learn On! [Re: steelmonkey]
    #14512106 - 05/25/11 09:28 PM (12 years, 9 months ago)

Yeah once you get something fruiting you'll forget about your jars :smile:

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InvisibleHorizonSpawn
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Re: Gettin' My Learn On! [Re: FrequentFryer]
    #14536638 - 05/30/11 04:07 PM (12 years, 9 months ago)

One of my 5-grain cakes/jars is about 90% colonized!...  with cobweb :frown:

One of my best colonizing rye cakes/jars is also growing teal colored crap, up near the surface :frown:


Still have four of each, though. So here's to hoping for some future success :smile:


--------------------
Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:

Extras: Filter Print Post Top
InvisibleHorizonSpawn
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Re: Gettin' My Learn On! [Re: HorizonSpawn]
    #14640353 - 06/19/11 10:57 PM (12 years, 8 months ago)

My first 1/2 pint cakes are doing well  Of the remaining 8 of 10; seven are 90~100%. Not sure what is up with the eighth one; it looks healthy, but there is a section void of growth. I assume there may be some contamination I can not recognize going on in there... I'll probably let them ALL sit for another two weeks, to fully consolidate; yes?
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Same order, just rotated 180deg:




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I'll be keeping a close eye on those two "slower" jars... I'll assume they are tainted if I don't see any growth in those areas soon.


--------------------
Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:

Extras: Filter Print Post Top
InvisibleHorizonSpawn
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Re: Gettin' My Learn On! [Re: HorizonSpawn]
    #14645481 - 06/20/11 09:44 PM (12 years, 8 months ago)

Wow? Not a single comment on my recent pics? :frown: Not even a flame, dis or bash?

:uhoh:


--------------------
Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:

Extras: Filter Print Post Top
Invisiblehiggledy-piggledy
Registered: 08/24/10
Posts: 966
Re: Gettin' My Learn On! [Re: HorizonSpawn]
    #14645594 - 06/20/11 09:59 PM (12 years, 8 months ago)

hi, its me! :smirk:

they look great man.

no signs of contams, as far as i can see. if there are any, you will realize soon enough.

nothing to worry about.


let them finish and consolidate.

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InvisibleHorizonSpawn
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Re: Gettin' My Learn On! [Re: higgledy-piggledy]
    #14645806 - 06/20/11 10:31 PM (12 years, 8 months ago)

Thank you, for the feedback :smile:


Still a lil nervous about those two jars... Only time will tell. :uhoh:


--------------------
Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:

Extras: Filter Print Post Top
InvisibleHorizonSpawn
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Re: Gettin' My Learn On! [Re: HorizonSpawn] * 1
    #14647161 - 06/21/11 07:27 AM (12 years, 8 months ago)

Anyone else have an educated observation to share?

Will the near fully colonized jars be okay for another two weeks? Or do you think I'll have to birth them sooner?


--------------------
Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:

Extras: Filter Print Post Top
OfflineProfessorPinHead
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Re: Gettin' My Learn On! [Re: HorizonSpawn]
    #14647300 - 06/21/11 08:30 AM (12 years, 8 months ago)

I would birth them sooner. Around 7 days after they hit 100%....

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InvisibleHorizonSpawn
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Re: Gettin' My Learn On! [Re: ProfessorPinHead]
    #14647428 - 06/21/11 09:21 AM (12 years, 8 months ago)

Then perhaps in 6~7 days, I'll birth and dunk those lil myco-cakes :smile:


--------------------
Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:

Extras: Filter Print Post Top
InvisibleHorizonSpawn
Gettin' my grow on :)
Male

Registered: 05/14/11
Posts: 1,739
Loc: Myco-Tek.org Flag
Re: Gettin' My Learn On! [Re: HorizonSpawn]
    #14662200 - 06/23/11 09:00 PM (12 years, 8 months ago)

I do see what appears to be a few micro-pins in two jars. Furthermore; seems the substrate has shrunk quite a bit...  Think I should birth and soak?...  Or wait until Monday?...  I'll be gone from Friday afternoon til Sunday Evening...  Hmmmmmmmmm...

:uhoh:


--------------------
Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:

Extras: Filter Print Post Top
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Shop: Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   MagicBag.co All-In-One Bags That Don't Suck   Unfolding Nature Unfolding Nature: Being in the Implicate Order


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