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Offlinemickle207
Stranger Shrooms

Registered: 09/03/10
Posts: 164
Last seen: 4 years, 4 months
Agar work
    #14494174 - 05/22/11 01:45 PM (12 years, 9 months ago)

Ok I've finally managed to (I think) perform agar work and I have some questions.

1. Refer to the petris that arent labelled.. 2 petris that I havent knocked up seems to have myc growth! Is it myc or is it a contam? It looks just like myc (to me) but it cant possibly be since never knocked them up.

2. The "OR Transfer" petri was knocked up at the center and took a while to grow; but after about 1 week the outer part that wasnt knocked grew exponentially and it certainly does not look like a contam or is it?








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Offlinemickle207
Stranger Shrooms

Registered: 09/03/10
Posts: 164
Last seen: 4 years, 4 months
Re: Agar work [Re: mickle207]
    #14502791 - 05/24/11 03:32 AM (12 years, 9 months ago)

No one?

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OfflineRogerRabbitM
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Re: Agar work [Re: mickle207]
    #14503093 - 05/24/11 06:36 AM (12 years, 9 months ago)

Contamination.  Any petri dish that is not wrapped in parafilm and/or touched with bare hands is going to become contaminated.

My method is to pour and work with petri dishes in front of a laminar flow hood while wearing surgical mask and gloves.  Then, wrap with parafilm, and while in front of the flow hood, each dish is placed into a ziplock bag, which is then inflated with sterile air from the flowhood. This provides a double barrier of sterile protection around the mycelium.
RR


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InvisibleSpector
Just Disappeared

Registered: 05/13/11
Posts: 167
Loc: A Scientific Location....
Re: Agar work [Re: RogerRabbit]
    #14503333 - 05/24/11 08:25 AM (12 years, 9 months ago)

yes, contam. its kinda commen sense.
tho, i wonder what kind of contam.
the most comment and fastest to contam,
for me at least is trich,
i dont see any color yet though.
it would of showed up by now?

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InvisibleDoc_T
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Registered: 03/06/09
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Re: Agar work [Re: Spector]
    #14503340 - 05/24/11 08:28 AM (12 years, 9 months ago)

I use gloves and ziploc bags, but yeah, you gotta seal them somehow.


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Offlinemickle207
Stranger Shrooms

Registered: 09/03/10
Posts: 164
Last seen: 4 years, 4 months
Re: Agar work [Re: Doc_T]
    #14503658 - 05/24/11 10:25 AM (12 years, 9 months ago)

I use parafilm to seal my petris inside a glovebox. You wont see them in the pics cos they were taken 'after' the transfer and are ready to be thrown away.

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