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Offlinenemsko
The Dude
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Registered: 05/17/11
Posts: 3
Last seen: 11 years, 5 months
(Question) PF Tek 100% Bacteria Contamination
    #14502586 - 05/24/11 01:42 AM (12 years, 8 months ago)

Hi, I recently attempted my first grow using the PF Tek. Unfortunately a week after inoculating All of my jars showed signs of bacterial growth at the point of inoculation. There was a white/gray slime growing on the sides of the jars where the inoculate hit the glass surrounded by uncolonized substrate with rings of healthy myc growth around it. I followed each step carefully, steam sterilizing for 90 minutes, using a glove box and flame sterilizing between each jar, ect. I'm going to attempt again and was wondering what are the common causes of bacterial contamination and any additional steps I can take to prevent this from happening a second time?


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Offlinemellowparty
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Registered: 05/17/09
Posts: 18,467
Last seen: 10 years, 2 months
Re: (Question) PF Tek 100% Bacteria Contamination [Re: nemsko]
    #14502643 - 05/24/11 02:07 AM (12 years, 8 months ago)

Assuming you did everything as you're instructed then my guess is that your LC/spore suspension was fucked up. Change it and try again.


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Offlinenemsko
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Registered: 05/17/11
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Re: (Question) PF Tek 100% Bacteria Contamination [Re: mellowparty]
    #14502665 - 05/24/11 02:20 AM (12 years, 8 months ago)

Suspension? Do you mean solution? Either way I used a syringe ordered from spores 101. I have one more left from the same order. Wonder if I should use it or order from another vendor.


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Offlinemellowparty
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Registered: 05/17/09
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Re: (Question) PF Tek 100% Bacteria Contamination [Re: nemsko]
    #14502683 - 05/24/11 02:28 AM (12 years, 8 months ago)

In chemistry, a suspension is a heterogeneous fluid containing solid particles that are sufficiently large for sedimentation. I've done inoculations in much shittier environments, like my mate's dirty table where it was full of bread crumbs and food :lol: And it worked at a 100% rate. So yeah get new inoculation material.


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Offlinenemsko
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Registered: 05/17/11
Posts: 3
Last seen: 11 years, 5 months
Re: (Question) PF Tek 100% Bacteria Contamination [Re: mellowparty]
    #14504700 - 05/24/11 02:03 PM (12 years, 8 months ago)

Wish I was that lucky. When I cleaned my jars out they smelled like burnt hair wrapped in a dirty diaper, It was unpleasant. I Have them soaking in a bucket of bleach and water.


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