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Offline3n1gm4
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Picking sectors to transfer to get an isolate to test.
    #14497577 - 05/23/11 04:05 AM (12 years, 9 months ago)

I understand at least part of this

Most mushrooms produce spores. When a mushroom spore germinates, the mycelium that emerges from the spore generally has one nucleus per cell, and we refer to this as hyphae, or monkaryotic mycelium. When hyphae that emerged from two individual spores of the same species cross paths, if they’re compatible, they join together to exchange DNA between them. The resulting mycelium generally has two or more nuclei per cell, and is referred to as dikaryotic mycelium. This mycelium, formed from two separate hyphae, is the very definition of a strain.
Since it only takes two spores to make a strain, you can easily see how many strains can potentially differentiate when you use an inoculating loop to swipe thousands of spores from a sporeprint onto a Petri dish of agar media. The process of strain isolation involves transferring these individual growths, or sectors away from each other, so that each can be expanded and fruited to determine the best performing strains. Strains with the desired characteristics will then become part of your permanent collection, stored long-term in test tubes as master culture slants.

So what I am getting from that is that a single strand of rhizo growth is a single strain and you need to select the sectors with the rhizo growth that looks like it is twisted together because those strands are compatible strains and exchanging dna. So I should choose a sector that looks like a sector of rhizo growth that has all the rhizo growth twisted together to transfer compatible strains to agar until I get a roung connected rhizo culture with no stray myc and all the rhizo growth looks like it is entwined. I then transfer a sector to grain and do GLC or GTG when it colonizes and then I will be growing a true isolae to test out. If it is Bunk+ I start over? Am I close at least?


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OfflineTerry M
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Re: Picking sectors to transfer to get an isolate to test. [Re: 3n1gm4]
    #14497761 - 05/23/11 05:58 AM (12 years, 9 months ago)

Quote:

3n1gm4 said:
So what I am getting from that is that a single strand of rhizo growth is a single strain and you need to select the sectors with the rhizo growth that looks like it is twisted together because those strands are compatible strains and exchanging dna.




The way I understand it, a true single sector results from the "mating" of a single pair of sexually complimentary mycelium strands. They are genetically a single strain, rather than a mixture of strains. The DNA is the same as would be found in the clone of a single mushroom.

And it shouldn't matter whether the sector looks twisted together or not. If it is from a single pairing of (haploid) primary mycelium, it  is one strain.

Someone, please correct me if I'm wrong, as I would like to understand this fully as well!


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OfflineRogerRabbitM
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Re: Picking sectors to transfer to get an isolate to test. [Re: Terry M]
    #14497790 - 05/23/11 06:16 AM (12 years, 9 months ago)

Rhizomorphic mycelium isn't genetic.  It's a trait, and you can stimulate it to grow with a weak media solution.

Transfer all sectors until they're isolated and then grow each one for testing.
RR


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