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InvisibleSpector
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Registered: 05/13/11
Posts: 167
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Re: Is this the way to get potent mushrooms? [Re: Spector]
    #14505736 - 05/24/11 05:38 PM (12 years, 8 months ago)

Quote:

RogerRabbit said:
Quote:

Sci-Fi said:
Agree. A clone is also an isolate, single strain, mono-culture. Same thing. You may have derived it via cloning a mushroom, but it's still a isolated strain.

Two or more strains creating one mushroom, together, without joining or exchanging genetic material?
That sounds like science fiction.




You are incorrect, as is scatmanrav.

There can be dozens of strains conspiring to form a single fruiting body, without these strains combining into a single organism.  This is well known and established in mycology.  Paul Stamets reports isolating up to 200 organisms from a single fruit.  It's not science fiction, it's science.  Picture thousands of individual soldiers in an army going to battle.  Each is separate, yet conspire to form the coherent fighting force.

An isolate can either come from a pairing of two compatible hyphae from separate spores, or from mycelium that has later combined via anastomosis into a single organism.  There can be monokaryotic pairings with other mononucleate mycelium, dikaryotic pairings with other multinucleate mycelium, or mono-dikaryotic pairings.  You know you have an isolate when there is no more sectoring on agar.

To the OP, rhizomorphic mycelium is not the only characteristic we isolate for.  The important thing is to get to single sectors and then fruit out each strain.  This is the only proved way to find the best performing strains.  Many rhizomorphic strains will be poor performers, but some will blow your socks off.  The latter are the ones you keep, discarding the former.  The only way to know is to fruit each one.  It's a long process, but it's fun and how we come up with the best commercial strains.
RR



So you can clone, in the beginning. but once you start Isolating strains sectors, you start using em as master cultures to cultivate fruits. keep the ones you like and discard the ones you dont. you keep isolating off that master culture. you dont clone off the fruits you've cultivated from that master, you can... but the master Culture already contains those traits in the sector you have stored. cloning would be like throw ur neatly folded clothes on the floor, as an example, were as sectors, with-in that master sector are neat and clean. So if you get to a point were you cant isolate off a sector anymore that is when its truely isolated, and cloning off that truely isolated sector, maybe cause othere sectors, or just mix everything up again. yea? This may sound confusing cuz i use isolate and sectors alot. but read, and re-read it. or maybe sumone get clarify,


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Invisiblefloatindownsligo
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Registered: 02/07/10
Posts: 118
Re: Is this the way to get potent mushrooms? [Re: Spector]
    #14505765 - 05/24/11 05:46 PM (12 years, 8 months ago)

yep you got the right idea. either way, on the hunt to a more potent mushroom your going to be doing a lot of work w/ petri dishes. might as well start them from spores so you can find the greatest diversity and see the health of the culture from spore to fruit.


--------------------
the meaning of life is to live it


Edited by floatindownsligo (05/24/11 05:47 PM)


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