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OfflineTheThirdEye
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Registered: 06/08/10
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First grow ever started 5/14/11. BRF PF tek p. Menace grow. Invitro.
    #14450034 - 05/14/11 08:25 AM (12 years, 9 months ago)

Alright well starting the first grow today.
It's going to be 3 BRF 1/2 pints wide mouth jars and a couple shot glasses.
The syringes were made in a small bathroom with no glovebox so hopefully they aren't contaminated. Planning to mark the three syringes and inoc the jars with one each inorder to tell which ones might be contaminated.
Well that's about it for now. Going to get started.
First grow is P. Menace strain and maybe Blue Meanies during this operation midway.
Special thanks to MeatWodd7600, Traikun, and Doc_T for great help and advice!
Will update picture soon.

Edited by TheThirdEye (05/20/11 12:51 PM)

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OfflineTheThirdEye
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Re: Starting First Grow TODAY! [Re: TheThirdEye]
    #14450179 - 05/14/11 09:17 AM (12 years, 9 months ago)


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Offlinek00laid
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Re: Starting First Grow TODAY! [Re: TheThirdEye]
    #14450688 - 05/14/11 11:26 AM (12 years, 9 months ago)

Quote:

TheThirdEye said:
Planning to mark the three syringes and inoc the jars with one each inorder to tell which ones might be contaminated.




very well thought out.

but just a suggestion. most first timers use syringes from sponsors instead of making their own. but more power to ya if you can make clean syringes your first time

you'll go far :thumbup:


--------------------
AMU - AMU Q & A - MyVideo Teks!

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OfflinePuzzled
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Registered: 03/28/11
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Re: Starting First Grow TODAY! [Re: TheThirdEye]
    #14450729 - 05/14/11 11:33 AM (12 years, 9 months ago)

Quote:

TheThirdEye said:
The syringes were made in a small bathroom with no glovebox so hopefully they aren't contaminated.




Bathrooms have the most stuff floating around in them of anywhere in a house.  You're probably fine, but next time, try to do it somewhere that gets less moisture, like a desk in your bedroom.

Good luck on your grow.


--------------------
Everything I say is just general thoughts and should not be taken as advice, recommendations, or suggestions.  If I write "you," it's as an indefinite personal pronoun because I find the use of "one" too formal and tiresome.

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Offlinek00laid
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Re: Starting First Grow TODAY! [Re: Puzzled]
    #14450827 - 05/14/11 11:49 AM (12 years, 9 months ago)

Quote:

Puzzled said:
Quote:

TheThirdEye said:
The syringes were made in a small bathroom with no glovebox so hopefully they aren't contaminated.




Bathrooms have the most stuff floating around in them of anywhere in a house.




i would disagree :/

its the smallest and most enclosed space in most peoples houses.

meaning the air in it is not moving (life size still air box yo)

you just gotta clean your shit yo. just cuz your bathroom looks and smells like shit doesnt mean mine is.

fuck i'd eat off the floor of my bathroom.


--------------------
AMU - AMU Q & A - MyVideo Teks!

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OfflineTheThirdEye
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Re: Starting First Grow TODAY! [Re: k00laid]
    #14450858 - 05/14/11 11:53 AM (12 years, 9 months ago)

Thanks K00laid for the comments and a laugh haha
:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2: to you sir.

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OfflinePuzzled
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Re: Starting First Grow TODAY! [Re: k00laid]
    #14451260 - 05/14/11 01:06 PM (12 years, 9 months ago)

Quote:

you just gotta clean your shit yo. just cuz your bathroom looks and smells like shit doesnt mean mine is.




My bathroom is immaculate, and there is no need for insults.

Bathrooms have high(er) humidity.  That humidity provides better conditions for fungi than most areas of a house.  Even a well-cleaned bathroom is going to have more contaminates than a well-cleaned living room.


--------------------
Everything I say is just general thoughts and should not be taken as advice, recommendations, or suggestions.  If I write "you," it's as an indefinite personal pronoun because I find the use of "one" too formal and tiresome.

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Offlinek00laid
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Re: Starting First Grow TODAY! [Re: Puzzled]
    #14451285 - 05/14/11 01:09 PM (12 years, 9 months ago)

Quote:

Puzzled said:
Quote:

you just gotta clean your shit yo. just cuz your bathroom looks and smells like shit doesnt mean mine is.




My bathroom is immaculate, and there is no need for insults.

Bathrooms have high(er) humidity.  That humidity provides better conditions for fungi than most areas of a house.  Even a well-cleaned bathroom is going to have more contaminates than a well-cleaned living room.




again i 100% disagree, due the sheer volume of air we are working with.

lets say you have 10 square foot room. and a 100 square foot room.

you spray a bleach solution into the air to settle all airborne contams, this also traps them onto the surface they end up landing on because its still wet with your bleach solution.

now with the air "scrubbed" and the two rooms "exactly" the same amount of clean, which is more likely to have a single trichoderma spore floating around in the air.

is it the 100 cubic feet of air, or the 10 cubic feet of air.


--------------------
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Offlinesporesmores420
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Re: Starting First Grow TODAY! [Re: Puzzled]
    #14451348 - 05/14/11 01:21 PM (12 years, 9 months ago)

Quote:

Puzzled said:
Quote:

you just gotta clean your shit yo. just cuz your bathroom looks and smells like shit doesnt mean mine is.




My bathroom is immaculate, and there is no need for insults.

Bathrooms have high(er) humidity.  That humidity provides better conditions for fungi than most areas of a house.  Even a well-cleaned bathroom is going to have more contaminates than a well-cleaned living room.





This is true there is always mold in bathrooms from ppl taking showers, thats why your supposed to turn the fan on while you shower.not to mention there is a bowl of water evaporating in your toilet.


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OfflineTheThirdEye
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Re: Starting First Grow TODAY! [Re: k00laid]
    #14451367 - 05/14/11 01:24 PM (12 years, 9 months ago)

The bathroom is a two part bathroom. A door to the sinks and a disinfected flat surface and another door to the toilet and shower. Syringe was made in the sink area.

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OfflineApple core
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Registered: 03/21/09
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Re: Starting First Grow TODAY! [Re: TheThirdEye]
    #14451520 - 05/14/11 01:59 PM (12 years, 9 months ago)

I agree that bathroom inoculation is no problem, providing it's well cleaned, and you bleach-mist the heck out of the place and give it time to settle.

My general rule of thumb is if my eyes aren't slightly burning after a minute or so, then it's not enough. :laugh:

Also I disagree with inoculating in rooms with soft furnishings, there's too many nice fluffy surfaces for mould spores and their kind to wait around and plan their attack.

Bathrooms are designed to be wiped clean; you can't scrub up a living-room anywhere near as well.

I like your shot glass by the way, keep us posted with the yield :thumbup:

Edited by Apple core (05/14/11 02:01 PM)

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Offlinek00laid
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Re: Starting First Grow TODAY! [Re: Apple core]
    #14451563 - 05/14/11 02:08 PM (12 years, 9 months ago)

Quote:

Apple core said:
Bathrooms are designed to be wiped clean; you can't scrub up a living-room anywhere near as well.




:whathesaid:


--------------------
AMU - AMU Q & A - MyVideo Teks!

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OfflineTheThirdEye
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Re: Starting First Grow TODAY! [Re: TheThirdEye]
    #14451600 - 05/14/11 02:16 PM (12 years, 9 months ago)

Just got them sterilized.
Let them cool for an hour in the pc and took them out and they've been sitting for 2 hours now in 70 degrees.

Planning on inoculating in 6 more hours. Will keep you guys posted!

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Offlinepunkrocker292004
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Re: Starting First Grow TODAY! [Re: k00laid]
    #14451618 - 05/14/11 02:20 PM (12 years, 9 months ago)

Quote:

k00laid said:
Quote:

TheThirdEye said:
Planning to mark the three syringes and inoc the jars with one each inorder to tell which ones might be contaminated.




very well thought out.

but just a suggestion. most first timers use syringes from sponsors instead of making their own. but more power to ya if you can make clean syringes your first time

you'll go far :thumbup:





first time i made a syringe it was clean as the dickens:shrug:


--------------------
EVERYTHING YOU NEED TO KNOW

on a long enough timeline the survival rate for everyone drops to zero-tyler durden

watch me

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OfflineTheThirdEye
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Re: Starting First Grow TODAY! [Re: punkrocker292004]
    #14484284 - 05/20/11 12:55 PM (12 years, 9 months ago)

So on the sixth morning I found signs of life! My shot glass has 4 small spots of mycelium growth. I haven't checked up on my 1/2 pints yet but I'm sure they're doing well. I was pretty scared that my syringes would work because I didn't make them in a glovebox and they were really clear hut guess it worked... So far. Don't want to junk anything but I will post up pics in a day or two :smile:

Edited by TheThirdEye (05/20/11 02:38 PM)

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OfflineTheThirdEye
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Re: Starting First Grow TODAY! [Re: TheThirdEye]
    #14491349 - 05/21/11 08:40 PM (12 years, 9 months ago)

Just checked up on my shot glass and seems like a lot of the mycelium was hiding under the tape area! Also 2/3 of the 1/2 pints are showing growth. Seems like the syring i used for the shot glass and one of the jars is working best as in both has the most growth. I'd say 1-2weeks before full colonization. Can't wait for pins! (: Good Vibes.

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OfflineTheThirdEye
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Re: Starting First Grow TODAY! [Re: TheThirdEye]
    #14510770 - 05/25/11 04:39 PM (12 years, 9 months ago)

My shot glass is colonizing all around top bottom and one of my jars is colonizing fast. They were both from the same syringe, so I guess that was the good one. One of them has on small growth of mycelium on the bottom and the last none at all... Should I restart this jar or still wait a little longer? How long does it take to fully colonize usually?

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OfflineTheThirdEye
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Re: Starting First Grow TODAY! [Re: TheThirdEye]
    #14571150 - 06/06/11 07:45 PM (12 years, 8 months ago)

https://files.shroomery.org/files/08-32/836977153-mono.tx.d9.jpg

Hi an update here. I birthed my Shotglass 3 days ago and there is some knotting at the bottom and little around but some area has the long skinny stem looking mycelium like in the picture above. What is this? and how long till I see pinheads? =/

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