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Offlinexel
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Registered: 04/17/11
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Emergency question on my BRF jars!
    #14489798 - 05/21/11 02:19 PM (12 years, 9 months ago)

Here is a link to all the cakes just days ago when they were doing fine and looked perfect.  Nothing has changed about their environment or anything like that.  Right now I am noticing on maybe 4-5 of the 20+ jars or so that there are very small but noticible spots of either a tan, yellow, or orange color. ( I cant exactly decide between what color it is, consider it a hybrid yellow, tan, orange lol )

They are all almost at 100 percent colonization.  My question is should I throw these out all together or see what happens.  So far as a precaution I have isolated them in a seperate container from the other ones who seem to be doing just as well as they were in my previous thread.

http://www.shroomery.org/forums/showflat.php/Number/14446432

I will post new pics of the spots in question.  I am really hoping I didn't get this far with no bumps in the road and then all of sudeen lose it all :frown:

Posting pics soon just hoping to hear the bad news sooner then later




Edited by xel (05/21/11 03:34 PM)

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OfflineBuckeye Oysters
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Re: Emergency question on my BRF jars! [Re: xel]
    #14489809 - 05/21/11 02:22 PM (12 years, 9 months ago)

The yellow-orange substance is called metabolite and it is a antibacterial liquid made by the mycellium to protect itself.  It usually shows up if you are incubating too warm (should only be 75-80f) or got some contam in with the inoculation.  If it fully colonizes and doesnt have a funky sour smell then you should be alright to birth, dunk and roll it.


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Offlinexel
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Re: Emergency question on my BRF jars! [Re: Buckeye Oysters]
    #14489824 - 05/21/11 02:26 PM (12 years, 9 months ago)

okay sweet I instantly took it out of the incubator once I noticed it and now im exclusively keeping it at room temperature.  I appreciate the advice I will post pics and hopefully you can tell me more about it

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Offlinexel
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Re: Emergency question on my BRF jars! [Re: xel]
    #14489849 - 05/21/11 02:30 PM (12 years, 9 months ago)

also another question if the jars that have this metabolite on them are close to jars that dont have anything but whit myc in them will they contaminate the other jars?

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Offlinemabachko
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Re: Emergency question on my BRF jars! [Re: xel]
    #14489911 - 05/21/11 02:44 PM (12 years, 9 months ago)

They shouldn't if u used a verm barrier and or filter.
Get rid of the incubator.

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Offlinexel
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Re: Emergency question on my BRF jars! [Re: mabachko]
    #14490066 - 05/21/11 03:11 PM (12 years, 9 months ago)

I definitely used a dry verm layer and it definitely was isolated to 4-5 that were in the incubator that aren't anymore.  I'm setting up a couple photos right now to show you a closer look

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Offlinexel
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Re: Emergency question on my BRF jars! [Re: xel]
    #14490141 - 05/21/11 03:26 PM (12 years, 9 months ago)

okay here there are and this shows the most "severe" growth if it actually is severe at all. Whatever it is I caught it early enough and it seems to be isolated to these jars.  What do you guys think?


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Offlinexel
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Re: Emergency question on my BRF jars! [Re: xel]
    #14490251 - 05/21/11 03:50 PM (12 years, 9 months ago)

bump with the pictures can anyone confirm it really is metabolite and nothing nasty?

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Invisiblemister
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Re: Emergency question on my BRF jars! [Re: xel]
    #14490274 - 05/21/11 03:54 PM (12 years, 9 months ago)

The first jar in the first pic looks powdery on the bottom. But in my time I have never had good fortune with jars that have metabolites that early on in growth. Best advise I can say is wait it out and watch them.


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OfflineRogerRabbitM
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Re: Emergency question on my BRF jars! [Re: mister]
    #14490323 - 05/21/11 04:07 PM (12 years, 9 months ago)

The metabolites are the least of your problem.  I see bacteria contamination and at least two different mycelium types, which means at least one must be mold.
RR


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Offlinexel
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Re: Emergency question on my BRF jars! [Re: mister]
    #14490351 - 05/21/11 04:13 PM (12 years, 9 months ago)

awww man I really hope the whole bunch didnt get messed up.  I would hate to start over again but hey live and learn I was just really excited about this bunch :/

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OfflineMushaboom
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Re: Emergency question on my BRF jars! [Re: xel]
    #14490365 - 05/21/11 04:15 PM (12 years, 9 months ago)

It looks exactly like a bacterial contamination to me.


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"What you think, you become."

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Offlinexel
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Re: Emergency question on my BRF jars! [Re: xel]
    #14490366 - 05/21/11 04:15 PM (12 years, 9 months ago)

should I just let them do their thing or give it a few days to see if it gets worse... RR if you dont mind asking in which picture do you see the mold growing and would you happen to know what it is?

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Offlinexel
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Re: Emergency question on my BRF jars! [Re: xel]
    #14490373 - 05/21/11 04:17 PM (12 years, 9 months ago)

also if it is a contamination will that spread to all the other jars regardless of what I do to isolate or destroy them?

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Offlinexel
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Re: Emergency question on my BRF jars! [Re: xel]
    #14490381 - 05/21/11 04:18 PM (12 years, 9 months ago)

I can take pictures of any parts of the jars or any close ups that may help you diagnose this.  Just let me know if you need me to take a different picture of somesort

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OfflineMushaboom
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Re: Emergency question on my BRF jars! [Re: xel]
    #14490406 - 05/21/11 04:25 PM (12 years, 9 months ago)

I would toss those cakes if you are unsure about anything at this point. It's better not to risk contaminating anything else. That is just my opinion though. The other cakes should be fine since they are fully colonized; it's harder for them to become infected once the mycelium has fully established itself. I generally move any cakes with any discoloration to a different location away from the other colonizing jars.


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"What you think, you become."

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Offlinexel
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Re: Emergency question on my BRF jars! [Re: Mushaboom]
    #14490445 - 05/21/11 04:36 PM (12 years, 9 months ago)

at this point they are completely isolated from the others im just hoping the others dont start showing signs.  What happened if you birth cakes that have contams?

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Invisiblethor_ak
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Re: Emergency question on my BRF jars! [Re: Mushaboom]
    #14490451 - 05/21/11 04:38 PM (12 years, 9 months ago)

It looks like there is alot of moisture in your jars, do you have them sealed?


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Offlinexel
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Re: Emergency question on my BRF jars! [Re: thor_ak]
    #14490467 - 05/21/11 04:41 PM (12 years, 9 months ago)

they were only sealed with the foil on top now they just have holes leading to the dry verm layers i took the foil of today

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Offlinexel
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Re: Emergency question on my BRF jars! [Re: xel]
    #14490481 - 05/21/11 04:46 PM (12 years, 9 months ago)

well I guess I will just pray now and plan on working on a new set of jars :frown:(

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