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xel
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Emergency question on my BRF jars!
#14489798 - 05/21/11 02:19 PM (12 years, 8 months ago) |
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Here is a link to all the cakes just days ago when they were doing fine and looked perfect. Nothing has changed about their environment or anything like that. Right now I am noticing on maybe 4-5 of the 20+ jars or so that there are very small but noticible spots of either a tan, yellow, or orange color. ( I cant exactly decide between what color it is, consider it a hybrid yellow, tan, orange lol )
They are all almost at 100 percent colonization. My question is should I throw these out all together or see what happens. So far as a precaution I have isolated them in a seperate container from the other ones who seem to be doing just as well as they were in my previous thread.
http://www.shroomery.org/forums/showflat.php/Number/14446432
I will post new pics of the spots in question. I am really hoping I didn't get this far with no bumps in the road and then all of sudeen lose it all 
Posting pics soon just hoping to hear the bad news sooner then later

Edited by xel (05/21/11 03:34 PM)
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Buckeye Oysters
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Re: Emergency question on my BRF jars! [Re: xel]
#14489809 - 05/21/11 02:22 PM (12 years, 8 months ago) |
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The yellow-orange substance is called metabolite and it is a antibacterial liquid made by the mycellium to protect itself. It usually shows up if you are incubating too warm (should only be 75-80f) or got some contam in with the inoculation. If it fully colonizes and doesnt have a funky sour smell then you should be alright to birth, dunk and roll it.
-------------------- Evolution is Lamarckism in disguise. Adaptation never creates a new species or trait, but rather the new species/trait always existed within the parent DNA until circumstances allowed it to be activated. For instance, every wolf has the DNA for poodles, but that DNA would never be revealed without man selectively breeding for it.
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xel
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okay sweet I instantly took it out of the incubator once I noticed it and now im exclusively keeping it at room temperature. I appreciate the advice I will post pics and hopefully you can tell me more about it
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xel
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Re: Emergency question on my BRF jars! [Re: xel]
#14489849 - 05/21/11 02:30 PM (12 years, 8 months ago) |
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also another question if the jars that have this metabolite on them are close to jars that dont have anything but whit myc in them will they contaminate the other jars?
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mabachko
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Re: Emergency question on my BRF jars! [Re: xel]
#14489911 - 05/21/11 02:44 PM (12 years, 8 months ago) |
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They shouldn't if u used a verm barrier and or filter. Get rid of the incubator.
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xel
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Re: Emergency question on my BRF jars! [Re: mabachko]
#14490066 - 05/21/11 03:11 PM (12 years, 8 months ago) |
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I definitely used a dry verm layer and it definitely was isolated to 4-5 that were in the incubator that aren't anymore. I'm setting up a couple photos right now to show you a closer look
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xel
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Re: Emergency question on my BRF jars! [Re: xel]
#14490141 - 05/21/11 03:26 PM (12 years, 8 months ago) |
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okay here there are and this shows the most "severe" growth if it actually is severe at all. Whatever it is I caught it early enough and it seems to be isolated to these jars. What do you guys think?
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xel
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Re: Emergency question on my BRF jars! [Re: xel]
#14490251 - 05/21/11 03:50 PM (12 years, 8 months ago) |
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bump with the pictures can anyone confirm it really is metabolite and nothing nasty?
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mister
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Re: Emergency question on my BRF jars! [Re: xel]
#14490274 - 05/21/11 03:54 PM (12 years, 8 months ago) |
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The first jar in the first pic looks powdery on the bottom. But in my time I have never had good fortune with jars that have metabolites that early on in growth. Best advise I can say is wait it out and watch them.
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RogerRabbit
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Re: Emergency question on my BRF jars! [Re: mister]
#14490323 - 05/21/11 04:07 PM (12 years, 8 months ago) |
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The metabolites are the least of your problem. I see bacteria contamination and at least two different mycelium types, which means at least one must be mold. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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xel
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Re: Emergency question on my BRF jars! [Re: mister]
#14490351 - 05/21/11 04:13 PM (12 years, 8 months ago) |
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awww man I really hope the whole bunch didnt get messed up. I would hate to start over again but hey live and learn I was just really excited about this bunch :/
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Mushaboom
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Re: Emergency question on my BRF jars! [Re: xel]
#14490365 - 05/21/11 04:15 PM (12 years, 8 months ago) |
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It looks exactly like a bacterial contamination to me.
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xel
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Re: Emergency question on my BRF jars! [Re: xel]
#14490366 - 05/21/11 04:15 PM (12 years, 8 months ago) |
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should I just let them do their thing or give it a few days to see if it gets worse... RR if you dont mind asking in which picture do you see the mold growing and would you happen to know what it is?
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xel
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Re: Emergency question on my BRF jars! [Re: xel]
#14490373 - 05/21/11 04:17 PM (12 years, 8 months ago) |
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also if it is a contamination will that spread to all the other jars regardless of what I do to isolate or destroy them?
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xel
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Re: Emergency question on my BRF jars! [Re: xel]
#14490381 - 05/21/11 04:18 PM (12 years, 8 months ago) |
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I can take pictures of any parts of the jars or any close ups that may help you diagnose this. Just let me know if you need me to take a different picture of somesort
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Mushaboom
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Re: Emergency question on my BRF jars! [Re: xel]
#14490406 - 05/21/11 04:25 PM (12 years, 8 months ago) |
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I would toss those cakes if you are unsure about anything at this point. It's better not to risk contaminating anything else. That is just my opinion though. The other cakes should be fine since they are fully colonized; it's harder for them to become infected once the mycelium has fully established itself. I generally move any cakes with any discoloration to a different location away from the other colonizing jars.
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"What you think, you become."
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xel
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Re: Emergency question on my BRF jars! [Re: Mushaboom]
#14490445 - 05/21/11 04:36 PM (12 years, 8 months ago) |
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at this point they are completely isolated from the others im just hoping the others dont start showing signs. What happened if you birth cakes that have contams?
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thor_ak
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Re: Emergency question on my BRF jars! [Re: Mushaboom]
#14490451 - 05/21/11 04:38 PM (12 years, 8 months ago) |
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It looks like there is alot of moisture in your jars, do you have them sealed?
-------------------- "A smart man learns from his mistakes. A wise man, learns from the mistakes of others. A woman, makes you pay for these mistakes."
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xel
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Re: Emergency question on my BRF jars! [Re: thor_ak]
#14490467 - 05/21/11 04:41 PM (12 years, 8 months ago) |
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they were only sealed with the foil on top now they just have holes leading to the dry verm layers i took the foil of today
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xel
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Re: Emergency question on my BRF jars! [Re: xel]
#14490481 - 05/21/11 04:46 PM (12 years, 8 months ago) |
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well I guess I will just pray now and plan on working on a new set of jars (
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thor_ak
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Re: Emergency question on my BRF jars! [Re: xel]
#14490482 - 05/21/11 04:46 PM (12 years, 8 months ago) |
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Yea, usually you want to make sure they can breath some what. Some people say that tin foil can stop that, but I'm not to sure about how much. But, I always take it off after I PC. If you birth them with contams, they could contam the other cakes, if you isolated them, depending on what type of bacteria is growing, if they do produce something, I'm not sure if you would want to ingest it.
-------------------- "A smart man learns from his mistakes. A wise man, learns from the mistakes of others. A woman, makes you pay for these mistakes."
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xel
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Re: Emergency question on my BRF jars! [Re: thor_ak]
#14490493 - 05/21/11 04:49 PM (12 years, 8 months ago) |
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thanks for the advice at this point i think im going to start a new set of jars and keep a close eye on all of these. Worst case scenario I can get some practice with the next steps including birthing even if they re contaminated. I will just toss them once im finished with them. It shouldn't hurt to practice a little bit.
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thor_ak
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Re: Emergency question on my BRF jars! [Re: xel]
#14490514 - 05/21/11 04:54 PM (12 years, 8 months ago) |
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You can try to cut off the contaminated spots, it doesn't hurt to try!
-------------------- "A smart man learns from his mistakes. A wise man, learns from the mistakes of others. A woman, makes you pay for these mistakes."
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xel
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Re: Emergency question on my BRF jars! [Re: thor_ak]
#14490526 - 05/21/11 04:57 PM (12 years, 8 months ago) |
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can I remove them from the jar early and remove them? Or wait till im ready to dunk, roll and birth? Also if I do remove them now can I simply cut out the contams and then put it back in the jar?
I plan on breaking it up in a bulk substrate anyways I dont plan on birthing from cakes but I may have to if more get contaminated
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xel
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Re: Emergency question on my BRF jars! [Re: xel]
#14490591 - 05/21/11 05:19 PM (12 years, 8 months ago) |
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Okay I have gone through every jar thoroughly and even if they showed the slightest sign of that color by the naked eye I isolated them all in the same container. However there are still 5 golden teacher jars that are almost fully colonized that show NONE of that contam and their are also 7 jars of PF Classic Amazonians that also show NONE of the contam by the naked eye. I have those in a separate container and will keep a close eye on all of them.
So there are 12 jars that appear to be perfectly healthy and there an additional 8-10 including the 5 I posted earlier that are obviously showing signs of whatever that is... Some are BARELY showing but just to be safe I isolated them with the rest.
I am hoping the healthy ones will pull it out and make it
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xel
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Re: Emergency question on my BRF jars! [Re: xel]
#14493446 - 05/22/11 10:52 AM (12 years, 8 months ago) |
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UPDATE. I checked on the ones I suspected to contaminated and you guessed it overnight they all sprung up with similar spots. The 12 that I managed to salvage still appear to be perfect.
Should I go into emergency birthing mode and birth the ones I know for a fact are not contaminated. They are 95% or more colonized I hate to do it early but i dont want to lose out to the black plague. Can anyone give me any advice on this?
I could just birth them from cakes and isolate them somewhere safe
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mabachko
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Re: Emergency question on my BRF jars! [Re: xel]
#14493460 - 05/22/11 10:54 AM (12 years, 8 months ago) |
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Shit can the bad. don't open inside. Let good colonize 100% & then wait a week to consolidate then birth.
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Beer Ponger
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Re: Emergency question on my BRF jars! [Re: mabachko]
#14534611 - 05/30/11 06:51 AM (12 years, 7 months ago) |
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update?
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