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OfflinePrimal Call
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I'm a teapot


Registered: 09/05/10
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Re: How can I improve my sterile technique for monotubs? [Re: TheAnimate]
    #14507014 - 05/24/11 09:33 PM (12 years, 8 months ago)

Don't blame the coffee! It's a great additive, IMO. I know monotubgirl uses the bucket tek with coffee and has no problems, but I think temperature- and time-measure pasteurization are assuredly a better option, regardless of substrate choice.

Here's some pasteurization links if you suspect that might be the problem.
Agar's pasteurization
Facey's Shitty Sub Prep

I always clean tubs prior to spawning with hot/soapy water then spray with Lysol disinfectant in the tub and in the space I'm going to use to spawn. Only trich problem I've had was with the bucket tek with my 1st project when I didn't have a thermometer to check temps. Noob mistake on my part.

I think if you had contaminated spawn, you would know. :shrug: Furthermore, I've fruited bulk that used contaminated spawn and still got two flushes before I decided it was not worth the risk to keep around.

:goodluck: troubleshooting this one... trich is infamously horrendous!

:peace:


--------------------
New Cultivator's Guide
Time to fruit? Pinning Strategy and Troubleshooting
My Trade Thread (Fungus, Plants, Herbal Medicine)


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