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Offlineitsahblunt420
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Registered: 11/04/10
Posts: 27
Last seen: 12 years, 11 months
why are my jars colonizing slowly/ not at all? using micro-supply spore syringes
    #13434650 - 11/04/10 03:36 PM (13 years, 3 months ago)

Hey guys, this is my first time so I don't know what I may have done wrong.

1) I mixed a ratio of 2:1:1 vermiculite:organic BRF: tap water
2) filled 6 1/2 pint jars up to the threading on the jars, slightly flattened with a spoon (barely any pressure), wiped inside the threaded area with a paper towel, filled to top with dry vermiculite, capped it tightly, lids each had 4 holes covered with breathable tape. covered top of jars with 6x6" aluminum foil, and pressure cooked them at 15-17 psi for 1 hour.
3) 24 hours after removing cooker from heat, i opened it, and with alcohol sterilized gloves I took them out and placed them in a glovebox i made out of a cardboard box and syran wrap. I brought my syringes into the box in their bag, opened it inside the box. i shook the syringe after taking it out of the bag. I flame sterilized the needle between jars, and after each flame sterilization, i wiped the black residue off of the needle with a paper towel soaked in iso alcohol. i injected 1cc of spore solution in each jar, so about 1/4cc per hole. when i was done, i took them out of the glove box, and put them in another cardboard box, and placed it on a shelf around only 70 degrees.

After 5 days, 2 of the jars had growth. one had 1 spot of growth, and has been growing relatively fast. the other one with growth has 2 spots, but both are growing much slower than the other one. the remaining four have shown no growth, and its now been 13 days since inoculation. 4 days ago, using the same ratios and everything i inoculated 4 more 1 pint jars. this time, i shook the syringe between each jar, and didnt wipe the residue off of the needle, thinking the alcohol may have killed the spores somehow. I have yet to see growth in these, however, it has only been 4 days.

I opened up and broke up the contents of one of the first jars that did not show signs of growth, and i found absolutely zero colonization anywhere within the substrate.

the 6 1/2 pint jars and the 4 pint jars were inoculated with Golden Teacher from micro-supply btw

Yesterday i inoculated 6 pint jars using the same method with African Transeki from micro-supply.

total time after inoculation of first batch is 13 days. the first 8 days, the temp ranged from 68-74 degrees. i then bought a heater, which happens to be hard to keep constant temps with. the temp has since been 78-84 degrees. at one point it got all the way up to 91 degrees and i immediately turned the heat off.

i haven't really given the 2nd and 3rd batch enough time to show growth, being inoculated only 4-5 days ago. I took the breathable tape off of each jar b/c i heard it helps and the dry vermiculite should keep contaminants out.

but why did only 3 of 24 inoculation sites show growth? i've heard mixed reviews of micro-tech and golden teacher in general, but the spore syringe had no clumps of spores in it, so i feel like the spores must have been evenly mixed in. even if the syringe had a low concentration of spores, shouldnt all of the jars still be colonizing at the same rate? Im planning to buy more syringes, but now im skeptical to purchase from micro-supply again. I'm hoping someone could figure out that i messed it up because id love to take advantage of $10 syringes assuming they actually have plenty of spores.

should i toss the other 3 1/2 pint jars? or should i try to re-inoculate them? or should i wait another week? i haven't seen any contaminants yet, so i guess im not doing too bad.

thanks for any advice


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Invisiblebourndead
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Registered: 07/06/10
Posts: 1,030
Re: why are my jars colonizing slowly/ not at all? using micro-supply spore syringes [Re: itsahblunt420]
    #13434732 - 11/04/10 03:53 PM (13 years, 3 months ago)

*obligatory order from sponsors remark*

There are plenty of sponsors that you'll get just as good a deal, if not better, from. Ralph gives away a free "strain of the month" w/ order upon request, and has package deals, as do others.

Aside from that, it can take 3-14days to see growth, ymmv, toss the ones over 2 weeks or sit on them a bit longer, your choice. Whatever you do decide, I'd be already on obtaining more supplies from a more reputable supplier if I were you.


--------------------
"I don't have a God complex, you've got a simple God"


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Offlineitsahblunt420
Stranger

Registered: 11/04/10
Posts: 27
Last seen: 12 years, 11 months
Re: why are my jars colonizing slowly/ not at all? using micro-supply spore syringes [Re: bourndead]
    #13434754 - 11/04/10 03:58 PM (13 years, 3 months ago)

I've been hearing good things about ralph, i just don't understand why the exact same spores in the exact same condition wouldn't grow exactly the same. i guess ill order from ralph instead. is it possible that first sign of growth could take more than 2 weeks?

whats wrong with mentioning non-sponsors? especially when im talking about problems with them.


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Offlinephayze
KNOWLEDGE SEEKER
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Registered: 09/05/10
Posts: 96
Loc: LEIGH LANCS UK Flag
Last seen: 13 years, 3 months
Re: why are my jars colonizing slowly/ not at all? using micro-supply spore syringes [Re: itsahblunt420]
    #13434840 - 11/04/10 04:15 PM (13 years, 3 months ago)

most living things grow differently even if they are grown under the same conditions its how things evolve,


--------------------
IF WE DIDNT KNOW WHERE WE HAD BEEN WE WOULDN'T KNOW WHERE WE ARE GOING


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OfflinePhishXIII
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Registered: 04/30/11
Posts: 63
Last seen: 3 years, 8 months
Re: why are my jars colonizing slowly/ not at all? using micro-supply spore syringes [Re: itsahblunt420]
    #14485071 - 05/20/11 03:40 PM (12 years, 8 months ago)

Quote:

itsahblunt420 said:

whats wrong with mentioning non-sponsors? especially when im talking about problems with them.




He's just saying the sponsors are trustworthy.


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