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Offlineshroomzi8
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Registered: 10/04/10
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stalled colonization
    #14466955 - 05/17/11 08:56 AM (12 years, 9 months ago)

PE on rye. 10 days in to colonization all going well. broke up rye. all seems to have stopped 7 days after breaking up and nowt. all seems to have gone. all the myc that was there aint no more. ideas?

mahalos.

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InvisibleSillicybin
Registered: 02/14/05
Posts: 2,134
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Re: stalled colonization [Re: shroomzi8]
    #14467252 - 05/17/11 10:30 AM (12 years, 9 months ago)

That is a long time for it to have not recovered.  It should have visibly recovered within 2 days.

What does your filter setup look like?  Can you post pictures?

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InvisibleEvilMushroom666
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Re: stalled colonization [Re: Sillicybin]
    #14467279 - 05/17/11 10:35 AM (12 years, 9 months ago)

Quote:

Sillicybin said:
That is a long time for it to have not recovered.  It should have visibly recovered within 2 days.

What does your filter setup look like?  Can you post pictures?



:whathesaid:

As well, when a grain jar is shaken up and does not recover, that is
usually a sign of some form of contamination, or problems with filted
gas exchange IMO.

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Offline3n1gm4
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Re: stalled colonization [Re: EvilMushroom666]
    #14467438 - 05/17/11 11:21 AM (12 years, 9 months ago)

It could also be a sign of too much moisture, I had a few rye jars that did the same thing and they were a little on the moist side because after the shake there was visible water droplets between the jar and the rye, see any moisture?:shrug:

I forgot to use gypsum in those mentioned jars, have never had a problem when I soak and boil with gypsum, they shake and break up much easier and also colonize faster when you use gypsum.


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Edited by 3n1gm4 (05/17/11 11:23 AM)

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Offlineshroomzi8
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Re: stalled colonization [Re: 3n1gm4]
    #14477062 - 05/19/11 06:10 AM (12 years, 9 months ago)

this one is an autoclave bag of rye. sealed with an innoc point. done a few of these b4 and never a prob. if i post a pic it will be of a plastic bag with some uncolonized grain in it.
it just stalled after breaking it up and hasnt re started. no contams, temp correct everyhting as normal.???

mahalos

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InvisibleSillicybin
Registered: 02/14/05
Posts: 2,134
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Re: stalled colonization [Re: shroomzi8]
    #14477090 - 05/19/11 06:22 AM (12 years, 9 months ago)

Quote:

shroomzi8 said:
this one is an autoclave bag of rye. sealed with an innoc point.




What's its means of gas exchange?  It has a filter patch, right?

Edited by Sillicybin (05/19/11 06:47 AM)

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Offlineveda_sticks
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Re: stalled colonization [Re: shroomzi8]
    #14477114 - 05/19/11 06:29 AM (12 years, 9 months ago)

Quote:

shroomzi8 said:
this one is an autoclave bag of rye. sealed with an innoc point. done a few of these b4 and never a prob. if i post a pic it will be of a plastic bag with some uncolonized grain in it.
it just stalled after breaking it up and hasnt re started. no contams, temp correct everyhting as normal.???

mahalos




no sign of contamsn, means yuou just cant see it. which would likely mean bacteria.

it could just be a bad filter bag or contamination occured during inoculation, or maybe your spore syringe or liquid culture you used is contaminated


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Offlineshroomzi8
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Re: stalled colonization [Re: veda_sticks]
    #14477249 - 05/19/11 07:20 AM (12 years, 9 months ago)

look it up at myco-farm.com. ive done a couple of these just because its quick and easy.

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