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babz89
engrossed:mycophile



Registered: 07/07/09
Posts: 895
Loc: PNW!
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I would recommend cauterizing the uncolonized parts to avoid contams (getting a butter knife red hot and then cutting off the uncolonized portion) don't worry about those pins if you're careful they'll still grow...
-------------------- "When the war of love overcomes the love of war, the world will truely be a better places. One love"Bob Marley
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ShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~




Registered: 07/18/10
Posts: 8,139
Loc: EVERY WHERE
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Re: First time grow - Pink Buffalo [Re: jazzaero]
#14500038 - 05/23/11 04:54 PM (12 years, 8 months ago) |
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Quote:
prismism said: You don't need to be a "cake guy" to help this gentleman out. I would have birthed those jars the day I saw the pins growing in vitro. The formation of fruiting bodies is telling you one of two things- Either your substrate is fully colonized or the substrate is contaminated and it is fruiting as a last ditch effort to reproduce. Either way, you take it out of the jar.
i do too. and if ur careful and dont knock the pins off they will be monsters.
Quote:
jazzaero said: It doesn't seem to be what looks like bacteria, but I'm no expert by any means. The cakes did shrink however and there is condensation on the inside of the jars. Could this have caused the pinning. I really do hope it's not bacteria. I'm hoping that if it is I can just wash off the cakes well before and after dunk and maybe get rid of it enough to let the mycelium take over.
Thanks for the responses everyone. Ill get some pics up once they are in the FC.
when they shrink it allows for a good Micro environment. thus the pinning.i leave my pins on the cake when i dunk. after u dunk, rince and roll!! the two mushies on my x7x cakes was pins that started in the jar.
i dont know why but the x7x just dont give good first flushes

good luck!
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jazzaero
Still just a spore



Registered: 04/29/11
Posts: 91
Last seen: 8 years, 8 months
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Re: First time grow - Pink Buffalo [Re: ShadOWCrack]
#14500964 - 05/23/11 08:05 PM (12 years, 8 months ago) |
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I dunked before I read these last two. So I didn't cauterize the uncolonized portions but I did wash them away basically. I ran water over them until they washed away and left the mycelium. Left on all the little tiny pins that would stay on. I will roll them tomorrow at about 4:00pm my time.
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punkrocker292004
i am a liar



Registered: 12/17/09
Posts: 2,921
Loc:
Last seen: 2 years, 4 months
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Re: First time grow - Pink Buffalo [Re: jazzaero]
#14502239 - 05/24/11 12:16 AM (12 years, 8 months ago) |
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good luck man
-------------------- EVERYTHING YOU NEED TO KNOW on a long enough timeline the survival rate for everyone drops to zero-tyler durden watch me
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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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Re: First time grow - Pink Buffalo [Re: uncle_rico]
#14502383 - 05/24/11 12:49 AM (12 years, 8 months ago) |
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Quote:
uncle_rico said:

"mooo !" "mooo !"
"Look what our poo has created !"
Good luck MAN
LOL. Hey Uncle.
I have had to wash the uncolonized portion off before, and did so without problems. Did the cakes smell shroomy? (this is actually a useful indicator)
Now, get that poor Buffalo out of the sun!
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
Edited by Javadog (05/24/11 12:51 AM)
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ShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~




Registered: 07/18/10
Posts: 8,139
Loc: EVERY WHERE
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Re: First time grow - Pink Buffalo [Re: Javadog]
#14502432 - 05/24/11 01:03 AM (12 years, 8 months ago) |
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jazzaero
Still just a spore



Registered: 04/29/11
Posts: 91
Last seen: 8 years, 8 months
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Re: First time grow - Pink Buffalo [Re: ShadOWCrack]
#14502469 - 05/24/11 01:13 AM (12 years, 8 months ago) |
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The cakes did actually smell shroomy. I'm assuming your telling me that is a good thing? Fully colonized is what your saying it is a good indicator of?
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punkrocker292004
i am a liar



Registered: 12/17/09
Posts: 2,921
Loc:
Last seen: 2 years, 4 months
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Re: First time grow - Pink Buffalo [Re: jazzaero]
#14502608 - 05/24/11 01:52 AM (12 years, 8 months ago) |
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full colonization is just good means no contamination
-------------------- EVERYTHING YOU NEED TO KNOW on a long enough timeline the survival rate for everyone drops to zero-tyler durden watch me
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babz89
engrossed:mycophile



Registered: 07/07/09
Posts: 895
Loc: PNW!
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Re: First time grow - Pink Buffalo [Re: jazzaero]
#14504179 - 05/24/11 12:18 PM (12 years, 8 months ago) |
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Quote:
jazzaero said: I dunked before I read these last two. So I didn't cauterize the uncolonized portions but I did wash them away basically. I ran water over them until they washed away and left the mycelium. Left on all the little tiny pins that would stay on. I will roll them tomorrow at about 4:00pm my time.
I think it'll work out for ya. Just keep an eye out for contams as soon as you see it chuck it!(outside for an outdoor bed if you can ) So do you wanna trade prints?
-------------------- "When the war of love overcomes the love of war, the world will truely be a better places. One love"Bob Marley
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jazzaero
Still just a spore



Registered: 04/29/11
Posts: 91
Last seen: 8 years, 8 months
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Re: First time grow - Pink Buffalo [Re: babz89]
#14504629 - 05/24/11 01:50 PM (12 years, 8 months ago) |
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Quote:
So do you wanna trade prints? 
Sure do 
I'm gonna clean up and then roll and put in the FC in a bit. I'll post some pics of my setup when I'm finished.
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jazzaero
Still just a spore



Registered: 04/29/11
Posts: 91
Last seen: 8 years, 8 months
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Re: First time grow - Pink Buffalo [Re: jazzaero]
#14505193 - 05/24/11 03:54 PM (12 years, 8 months ago) |
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Here are my little cakes in the FC. I still need to cut a hole in the top of the box and put some plastic on it to make a window for light. I also need to tape off or put some dark fabric on the sides of the box. Keep those little guys growing up and not out. 
Luckily my friend was doing a tissue culture experiment in my basement and had a great grow tent set up. This makes a nice little place for me to put my FC and keep it away from contams and other unwanted pests. Makes for a great work place.
By the way: Tissue culture is amazing as well. You all should read up on it.
Welp heres the pics:

Also How far should I keep the light from the top of the box. I have a 6500k light hanging above just for work space light but I was going to put some chains on it and lower it to the box. g
Thanks for reading
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ShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~




Registered: 07/18/10
Posts: 8,139
Loc: EVERY WHERE
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Re: First time grow - Pink Buffalo [Re: jazzaero]
#14505594 - 05/24/11 05:16 PM (12 years, 8 months ago) |
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as close as you can get it, with out making it to hot. it deepens on your light. i have 2 of them and one gets a lil hot, so i have to move it higher.
looks good!
iam sorry, but i can tell what ur temp/RH is. i was just wandering.
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punkrocker292004
i am a liar



Registered: 12/17/09
Posts: 2,921
Loc:
Last seen: 2 years, 4 months
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Re: First time grow - Pink Buffalo [Re: ShadOWCrack]
#14505662 - 05/24/11 05:25 PM (12 years, 8 months ago) |
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nice set up bud might wanna put some hole in that or some big holes stuffed with polyfil
-------------------- EVERYTHING YOU NEED TO KNOW on a long enough timeline the survival rate for everyone drops to zero-tyler durden watch me
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ShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~




Registered: 07/18/10
Posts: 8,139
Loc: EVERY WHERE
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i think its in a humidity tent. u dont have to have them if it is.
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prismism



Registered: 05/11/09
Posts: 5,570
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Re: First time grow - Pink Buffalo [Re: jazzaero]
#14505815 - 05/24/11 06:00 PM (12 years, 8 months ago) |
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Fresh air is probably the most important thing for your cakes right now and you're giving them almost none. Take your chamber out of that tent thing you have it in, then drill holes on all six sides of your chamber. Then you'll be rockin' and rollin'.
-------------------- ephemeral anomalous
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jazzaero
Still just a spore



Registered: 04/29/11
Posts: 91
Last seen: 8 years, 8 months
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Re: First time grow - Pink Buffalo [Re: prismism]
#14506039 - 05/24/11 06:42 PM (12 years, 8 months ago) |
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It is in a tent but it is more just for somewhere to put the box out of the way. I don't think I can keep the entire tent high enough in humidity.
I do have four 1/4" holes drilled in the lid. There is also another hole next to the air tube inlet hole. I drilled two on accident. I was hoping this was enough for fresh air exchange. I am doing a PMP so i figured the air pump pushing air in and up out the top holes would be enough. If I am wrong I will drill more holes. I simply thought drilling holes all over the box was more for the shotgun terrarium.
My temp is spot on 70F and the RH is at 89%. The RH was at around 99% when I had a stronger pump on it, but it was for my friends organic tea brewer(the plants around the house LOVE it!) so I had to switch to MY smaller one. I am waiting to see if it goes up with the smaller pump since I just switched them out.
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babz89
engrossed:mycophile



Registered: 07/07/09
Posts: 895
Loc: PNW!
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Re: First time grow - Pink Buffalo [Re: jazzaero]
#14506143 - 05/24/11 07:03 PM (12 years, 8 months ago) |
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The pmp is kinda over teked and possible failure may insue. I'm not an expert but I was told I would save myself alot of trouble with a SGFC but who knows it might work out best of luck !
-------------------- "When the war of love overcomes the love of war, the world will truely be a better places. One love"Bob Marley
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prismism



Registered: 05/11/09
Posts: 5,570
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Re: First time grow - Pink Buffalo [Re: jazzaero]
#14506147 - 05/24/11 07:04 PM (12 years, 8 months ago) |
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I noticed that you were going for the pmp design, but I just want to let you know that there's no way you're getting enough fresh air from that little hose that's coming in the side there. That's the problem with pmps. I think you are misunderstanding what I'm trying to say with the tent. You don't need to humidifye the whole tent, you need to get rid of it. It seems like it would work great for inoculations and other sterile work, but your fruiting chamber area does not need to be sterile. You want your chamber to be in an area that has air moving. Placing your cakes in a tent that is in a closed box with six small holes and a tiny air pump is suffocating them.
-------------------- ephemeral anomalous
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jazzaero
Still just a spore



Registered: 04/29/11
Posts: 91
Last seen: 8 years, 8 months
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Re: First time grow - Pink Buffalo [Re: prismism]
#14506293 - 05/24/11 07:28 PM (12 years, 8 months ago) |
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Ok I see what your saying now. I will pull the box out of the tent then. Actually I will open the tent sides up as it is still a good work place for the box and it holds the light up well. Also the light won't be bothering anyone in there.
-Will I still be ok doing the PMP and just drilling more holes?
I am afraid the RH will go down if I drill too many holes. The PMP was tempting because of a positive air pressure keeping contams out. From what it sounds I do not need to worry about contams. PMP also seemed like a good idea because of less misting and fanning.
-Should I just forget the PMP and go shotgun or should I keep the PMP and hybrid to PMP/shotgun?
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prismism



Registered: 05/11/09
Posts: 5,570
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Re: First time grow - Pink Buffalo [Re: jazzaero]
#14506494 - 05/24/11 08:00 PM (12 years, 8 months ago) |
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You can keep the pump if you like the noise it makes, but it's really not doing much for you. It's a common misconception that adding more holes will lower the relative humidity, but the shotgun is designed so that air is drawn in up from the bottom of the tub through the moist perlite.
-------------------- ephemeral anomalous
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