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OfflineBigJohnson
Whoa! You guysneed to lightenup!

Registered: 02/04/03
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Multi-spore vs. Cloning
    #1447574 - 04/10/03 07:36 PM (13 years, 7 months ago)

I've been doing a lot of research lately on inoculating with mycelium water created by blending up a myc sample.

Now, we have all seen four distinct colonies radiating from the inoc points on a cake. These colonies are comprised of spores that have germinated in the substrate and formed a cooperative network comprised of numerous individuals.

My question is this: Can a pure (one set of genes) colony of myc be obtained from taking a sample of one of these colonies? Or, can one assume that this can only be achieved if the sample tissue is taken from a fruit stem?

If one took a small, rhizomorphic sample from one of these colonies, could he increase his chances of getting a colony with the same genes by doing or looking for something special?

From what I've read, yields can be almost doubled if your jars are colonized by a specific individual, as the individual gene set cooperates with "itself."

Your ideas and advice are appreciated :smile: 


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OfflineBigJohnson
Whoa! You guysneed to lightenup!

Registered: 02/04/03
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Last seen: 13 years, 5 months
Re: Multi-spore vs. Cloning [Re: BigJohnson]
    #1447818 - 04/10/03 09:12 PM (13 years, 7 months ago)

Anyone?


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Offlinearabmobster
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Registered: 04/01/03
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Re: Multi-spore vs. Cloning [Re: BigJohnson]
    #1447824 - 04/10/03 09:13 PM (13 years, 7 months ago)

yes


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OfflineGWAR
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Re: Multi-spore vs. Cloning [Re: BigJohnson]
    #1447837 - 04/10/03 09:17 PM (13 years, 7 months ago)

sounds cool..


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Offlinejakeholman
crotch rocketsrule!

Registered: 02/24/03
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Re: Multi-spore vs. Cloning [Re: GWAR]
    #1447887 - 04/10/03 09:36 PM (13 years, 7 months ago)

maybe you should try the advanced grow boards big J. but let me know for curiousity sake.


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Invisiblepoke smot!
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Re: Multi-spore vs. Cloning *DELETED* [Re: jakeholman]
    #1448069 - 04/10/03 10:45 PM (13 years, 7 months ago)

Post deleted by poke smot!

Reason for deletion: x



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Offlinetastyshroom
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Re: Multi-spore vs. Cloning [Re: poke smot!]
    #1448203 - 04/10/03 11:28 PM (13 years, 7 months ago)

When i took one of my cakes and blended em up to make a mycelium drink i decided to save in a little test tube i have(cap of course)
It's started growing mycelum under the water..it's starting to get bigger but growing slowly..so what your saying is that i could get a syringe suck up some of that water and inject it into jars of vermiculite and brown rice flour and it'll be like a spore syringe replacement and mycelium will spread?


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OfflineBigJohnson
Whoa! You guysneed to lightenup!

Registered: 02/04/03
Posts: 716
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Last seen: 13 years, 5 months
Re: Multi-spore vs. Cloning [Re: tastyshroom]
    #1448289 - 04/11/03 12:12 AM (13 years, 7 months ago)

Quote:

so what your saying is that i could get a syringe suck up some of that water and inject it into jars of vermiculite and brown rice flour and it'll be like a spore syringe replacement and mycelium will spread? 




Yep :smile:


--------------------
Should the US relegalize drugs?

http://www.lp.org/issues/relegalize.html


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OfflineBigJohnson
Whoa! You guysneed to lightenup!

Registered: 02/04/03
Posts: 716
Loc: The Yard
Last seen: 13 years, 5 months
Re: Multi-spore vs. Cloning [Re: BigJohnson]
    #1449455 - 04/11/03 01:13 PM (13 years, 7 months ago)

Cloning a bunch of different individuals would defeat the purpose of cloning the tissue to begin with. What I want is a huge mass of mycelium comprised of one individual gene set.

Anyone know if several individuals can form a rhizomorphic stand? Damn, where's my fricken' bio textbook when I need it...


--------------------
Should the US relegalize drugs?

http://www.lp.org/issues/relegalize.html


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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