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OfflineBabylon
Shaman

Registered: 05/15/11
Posts: 442
Last seen: 2 years, 9 months
Syringes
    #14473668 - 05/18/11 02:40 PM (12 years, 9 months ago)

So I have 3 different spore prints currently hydrating in water, I am planning on feeding them a bit of syrup to get a good liquid culture started and then injecting into jars PF style.  What i want to know is, do I need to get a load of syringes so that I can actually inject each separately?  Or will it work to use a meat injecting syringe and reuse it several times?

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InvisibleEvilMushroom666
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Registered: 11/18/09
Posts: 10,289
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Re: Syringes [Re: Babylon]
    #14473691 - 05/18/11 02:44 PM (12 years, 9 months ago)

You what?

How did you go about hydrating your spore prints?

What was your sterile procedure like?

Please do explain in more detail exactly what you have done upto this
point.

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OfflineBabylon
Shaman

Registered: 05/15/11
Posts: 442
Last seen: 2 years, 9 months
Re: Syringes [Re: EvilMushroom666]
    #14473716 - 05/18/11 02:48 PM (12 years, 9 months ago)

ok, so far I took 3 half pint mason jars and put them in an unpressurized pressure cooker with boiling water for 2 hours, they have approx 75 ml of water in them and the rest air.  Inside a glove box I scraped spore prints into those jars, then sealed them back up again. (this was after cooling them in sterile water poured into the pressure cooker)

The spore prints are a few years old, so I want to give them plenty of time to rehydrate, probably a week or so.

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InvisibleEvilMushroom666
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Folding@home Statistics
Registered: 11/18/09
Posts: 10,289
Loc: Canada Flag
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Re: Syringes [Re: Babylon]
    #14473736 - 05/18/11 02:51 PM (12 years, 9 months ago)

Not the most ideal of procedures but good luck none the less.

To answer your questions, you can purchase sterile syringes and 18G
needles from farm supply stores (TSC), that will be your best bet.

If all three spore prints are of a different variety then yes you would
want at least three syringes to draw your spore solution from. You
can re-use and sterilize syringes over and over again, use the search
bar for more information.

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