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BigPoppaSmurf
Stranger
Registered: 03/28/03
Posts: 23
Last seen: 20 years, 1 month
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Innoculating Grain w/ PF jar
#1446305 - 04/10/03 11:19 AM (20 years, 11 months ago) |
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Just wondering how I'd go about innoculating a grain substrate with a fully colonized PF jar. I should have 12 fully colonized PF jars by the end of this month, and I wanted to use one of them to innoculate a few quart or pint jars with it. I'm new to this process and want to move up to using grain or WBS. If someone could point me to a procedure that would be great.
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Uber
Wash ufungi,drive me tofruitenzi
Registered: 01/26/03
Posts: 121
Loc: DRC
Last seen: 19 years, 5 months
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Re: Innoculating Grain w/ PF jar [Re: BigPoppaSmurf]
#1446319 - 04/10/03 11:27 AM (20 years, 11 months ago) |
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Here's what my FOAF would do:
Take fully colonized jar and move to glove box/positive pressure box. Sterilize outside of the jar and let dry. Open jar, then use blender tek (http://www.nansnook.com/archives/3409.html) to turn cake into powder. Take said powder and transfer to grain jars and shake well. My FOAF says he would use a ratio of 15:1 grain to powdered cake.
Most important thing to keep in mind is sterility. If you don't have a positive pressure box you should build one. At the very least use a glove box and keep things nice and clean inside with a bleach water mist or lysol (some ppl don't like to use lysol since it can cause mutations... my FOAF has used lysol without problems. YMMV).
-------------------- "The old adage that ignorance of the law is no excuse becomes a ludicrous statement when the laws themselves are rooted in ignorance" - Adam Gottlieb
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SixTango
Mycota
Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
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Re: Innoculating Grain w/ PF jar [Re: BigPoppaSmurf]
#1446455 - 04/10/03 12:09 PM (20 years, 11 months ago) |
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Uber's advice is good. You could also (suggest - in a glove box) simply open a colonized pf jar & with a (sterile) sharp edged tablespoon, munch up the content into tiny bits. Then, spoon around two (2) tablespoons into each WBS jar. Then close up the WBS jars & give then a good shake, then incubate. 6T
-------------------- ~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~
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BigPoppaSmurf
Stranger
Registered: 03/28/03
Posts: 23
Last seen: 20 years, 1 month
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Re: Innoculating Grain w/ PF jar [Re: SixTango]
#1449156 - 04/11/03 08:56 AM (20 years, 11 months ago) |
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Thanks for the info, but how would I go about creating a positive pressure box and what's the best way to sterilize a blender?
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Unknown
Confused,dizzy,wheream I again?
Registered: 01/06/02
Posts: 380
Loc: MA
Last seen: 20 years, 10 days
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Re: Innoculating Grain w/ PF jar [Re: BigPoppaSmurf]
#1449963 - 04/11/03 01:52 PM (20 years, 11 months ago) |
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Here you go,next time look in the FAQ and use the search function.
www.shroomery.org/index/par/7923/pag/1
You can use a hair dryer instead of the computer fan,just set the hair dryer on COOL air(mandatory),then cut a hole in a tupperware container just big enough to fit the hairdryer nozzle and then seal it.Then you tape a filter disc or hepa filter over the open end of the tupperware so it's sealed.When you turn it on it should blow clean air,then you put that whole thing in a rubbermaid or whatever you're using for the glovebox and seal the edges,and that's it for the "fan".Using a hair dryer is MUCH easier than finding the fan and all that.If you have a computer fan lying around use that,just don't bust your ass to go find one
-------------------- The above is just like,my opinion man
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BigJohnson
Whoa! You guysneed to lightenup!
Registered: 02/04/03
Posts: 716
Loc: The Yard
Last seen: 20 years, 8 months
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Re: Innoculating Grain w/ PF jar [Re: Anonymous]
#1450023 - 04/11/03 02:06 PM (20 years, 11 months ago) |
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Check out the 9er tek in the faq, advanced cult.
-------------------- Should the US relegalize drugs? http://www.lp.org/issues/relegalize.html
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Unknown
Confused,dizzy,wheream I again?
Registered: 01/06/02
Posts: 380
Loc: MA
Last seen: 20 years, 10 days
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Re: Innoculating Grain w/ PF jar [Re: BigJohnson]
#1450254 - 04/11/03 03:22 PM (20 years, 11 months ago) |
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The one I posted in the same one as in the 9er tek
-------------------- The above is just like,my opinion man
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