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OfflineBigPoppaSmurf
Stranger

Registered: 03/28/03
Posts: 23
Last seen: 12 years, 9 months
Innoculating Grain w/ PF jar
    #1446305 - 04/10/03 01:19 PM (13 years, 7 months ago)

Just wondering how I'd go about innoculating a grain substrate with a fully colonized PF jar. I should have 12 fully colonized PF jars by the end of this month, and I wanted to use one of them to innoculate a few quart or pint jars with it. I'm new to this process and want to move up to using grain or WBS. If someone could point me to a procedure that would be great.


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OfflineUber
Wash ufungi,drive me tofruitenzi

Registered: 01/26/03
Posts: 121
Loc: DRC
Last seen: 12 years, 1 month
Re: Innoculating Grain w/ PF jar [Re: BigPoppaSmurf]
    #1446319 - 04/10/03 01:27 PM (13 years, 7 months ago)

Here's what my FOAF would do:

Take fully colonized jar and move to glove box/positive pressure box. Sterilize outside of the jar and let dry. Open jar, then use blender tek (http://www.nansnook.com/archives/3409.html) to turn cake into powder. Take said powder and transfer to grain jars and shake well. My FOAF says he would use a ratio of 15:1 grain to powdered cake.

Most important thing to keep in mind is sterility. If you don't have a positive pressure box you should build one. At the very least use a glove box and keep things nice and clean inside with a bleach water mist or lysol (some ppl don't like to use lysol since it can cause mutations... my FOAF has used lysol without problems. YMMV).


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"The old adage that ignorance of the law is no excuse becomes a ludicrous
statement when the laws themselves are rooted in ignorance"

- Adam Gottlieb


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Innoculating Grain w/ PF jar [Re: BigPoppaSmurf]
    #1446455 - 04/10/03 02:09 PM (13 years, 7 months ago)

Uber's advice is good. You could also (suggest - in a glove box) simply open a colonized pf jar & with a (sterile) sharp edged tablespoon, munch up the content into tiny bits. Then, spoon around two (2) tablespoons into each WBS jar. Then close up the WBS jars & give then a good shake, then incubate. 6T :tongue:


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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OfflineBigPoppaSmurf
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Registered: 03/28/03
Posts: 23
Last seen: 12 years, 9 months
Re: Innoculating Grain w/ PF jar [Re: SixTango]
    #1449156 - 04/11/03 10:56 AM (13 years, 7 months ago)

Thanks for the info, but how would I go about creating a positive pressure box and what's the best way to sterilize a blender?


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OfflineUnknown
Confused,dizzy,wheream I again?
Registered: 01/07/02
Posts: 380
Loc: MA
Last seen: 12 years, 8 months
Re: Innoculating Grain w/ PF jar [Re: BigPoppaSmurf]
    #1449963 - 04/11/03 03:52 PM (13 years, 7 months ago)

Here you go,next time look in the FAQ and use the search function.

www.shroomery.org/index/par/7923/pag/1

You can use a hair dryer instead of the computer fan,just set the hair dryer on COOL air(mandatory),then cut a hole in a tupperware container just big enough to fit the hairdryer nozzle and then seal it.Then you tape a filter disc or hepa filter over the open end of the tupperware so it's sealed.When you turn it on it should blow clean air,then you put that whole thing in a rubbermaid or whatever you're using for the glovebox and seal the edges,and that's it for the "fan".Using a hair dryer is MUCH easier than finding the fan and all that.If you have a computer fan lying around use that,just don't bust your ass to go find one


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The above is just like,my opinion man


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OfflineBigJohnson
Whoa! You guysneed to lightenup!

Registered: 02/04/03
Posts: 716
Loc: The Yard
Last seen: 13 years, 5 months
Re: Innoculating Grain w/ PF jar [Re: Anonymous]
    #1450023 - 04/11/03 04:06 PM (13 years, 7 months ago)

Check out the 9er tek in the faq, advanced cult.


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Should the US relegalize drugs?

http://www.lp.org/issues/relegalize.html


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OfflineUnknown
Confused,dizzy,wheream I again?
Registered: 01/07/02
Posts: 380
Loc: MA
Last seen: 12 years, 8 months
Re: Innoculating Grain w/ PF jar [Re: BigJohnson]
    #1450254 - 04/11/03 05:22 PM (13 years, 7 months ago)

The one I posted in the same one as in the 9er tek


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The above is just like,my opinion man


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