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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale

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Offlinesporesmores420
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Help with LC
    #14453407 - 05/14/11 08:36 PM (12 years, 9 months ago)

can somone tell me if im gona fuk this up before i try it? I have half gallon, quart or the small wide mouth pf cake styles ones. I plan to pressure cook for 20 minutes. I have food club light corn syrup with real vanilla. I plan on buying rtv silicone and drilling a hole in the lid and then just using that to seal the hole. Then i will inoc them in a still air and let them cultivate in there.


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Offlineveda_sticks
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Re: Help with LC [Re: sporesmores420]
    #14453475 - 05/14/11 08:53 PM (12 years, 9 months ago)

bear in mind if you just have 1 hole that is sealed with silicione, once you pressure cook it, its going to have a vacum once cooled down.

If you dont equalise the pressure, as soon as you stick the syringe in, its gonna suck the contents of your sysringe into the jar.


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Offlinesporesmores420
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Re: Help with LC [Re: veda_sticks]
    #14453506 - 05/14/11 08:58 PM (12 years, 9 months ago)

how do i do the second hole then thats the part im having problems with?


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InvisibleYHWH
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Re: Help with LC [Re: sporesmores420]
    #14453522 - 05/14/11 09:02 PM (12 years, 9 months ago)

Well, you could stuff the hole with polyfil (very tightly), cover it with tyvek, or get some synthetic filter discs.

For my LCs I do two holes covered with silicone, then I just use a syringe minus the plunger stuffed with polyfil in one hole. Aspirate out of the other.


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OfflineWing
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Re: Help with LC [Re: YHWH]
    #14453635 - 05/14/11 09:30 PM (12 years, 9 months ago)

You could always PC your jars with a syringe in the inoc hole without the plunger. It would equalize pressure on its own once its cooled.


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Offlinesporesmores420
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Re: Help with LC [Re: Wing]
    #14455788 - 05/15/11 11:38 AM (12 years, 9 months ago)

im still confused why cant the hole from the inoc point equalize the pressure? and if not can i just rtv one hole and poly fill the other. then which one do i inoc thru?


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OfflineElevated
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Re: Help with LC [Re: sporesmores420]
    #14455807 - 05/15/11 11:42 AM (12 years, 9 months ago)

If you have two holes one with rtv one with poly, innoc in the rtv port so it heals itself.  As for the poly hole it should equilize the pressure in the jar. 

You could def do two holes like you said and just cover one of the holes with micropore tape and that should do it as well.

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Offlinesporesmores420
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Re: Help with LC [Re: Elevated]
    #14456834 - 05/15/11 03:13 PM (12 years, 9 months ago)

Quote:

Elevated said:
If you have two holes one with rtv one with poly, innoc in the rtv port so it heals itself.  As for the poly hole it should equilize the pressure in the jar. 

You could def do two holes like you said and just cover one of the holes with micropore tape and that should do it as well.




wont the polyfill be enough?


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OfflineElevated
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Re: Help with LC [Re: sporesmores420]
    #14457050 - 05/15/11 03:47 PM (12 years, 9 months ago)

yes

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