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Primz
Stranger
Registered: 05/15/11
Posts: 26
Last seen: 12 years, 7 months
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In need of a Pro
#14456405 - 05/15/11 02:00 PM (12 years, 8 months ago) |
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Hi everyone, i have read the FAQ`s back to back and im not sure if my brain has fryed from info overload or im just getting dumber with age but i am in need of some help from the pro`s.
I am growing mushrooms for the first time and i have my spore syringe (10ml`s) and my liquid culture vial (10ml`s) as there is already liquid in the culture vials how many ml`s do i add of my spores to the vial??
I know that mycelium will form in the vial and then i can suck up and inoculate my medium but in regard to this how much would i need to add ml wise to my grow box that are 1 liter large.
Thanks in advance people
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treewood69
person



Registered: 05/15/09
Posts: 606
Loc: omicron persei 8
Last seen: 7 years, 11 months
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Re: In need of a Pro [Re: Primz]
#14456480 - 05/15/11 02:15 PM (12 years, 8 months ago) |
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1ml
-------------------- I have enough cents to know I dont have any sense
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Carl Sagan
Time Dilation Analyst


Registered: 04/19/11
Posts: 922
Loc: Myco-tek.org
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Re: In need of a Pro [Re: Primz]
#14456491 - 05/15/11 02:17 PM (12 years, 8 months ago) |
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I use a 1/5 ratio. If you have 500ml add 1ml of MS.
What im not getting here is what is this LC vile you speak of. A test tube? What are you using for food. Has it been PC'd? Pics?
-------------------- “Sacred cows make the best hamburger” Mark Twain Independant Research Foundation
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awakendone


Registered: 08/05/10
Posts: 824
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Re: In need of a Pro [Re: Primz]
#14456517 - 05/15/11 02:25 PM (12 years, 8 months ago) |
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Quote:
Primz said: I am growing mushrooms for the first time and i have my spore syringe (10ml`s) and my liquid culture vial (10ml`s) as there is already liquid in the culture vials how many ml`s do i add of my spores to the vial??
it doesn't take much at all! for example, the first 2 LC's i made (100ml each). i used spore syringes that i had already completely used up all the liquid solution from. i just drew my LC into the empty spore syringes and shot it back into the LC. they came out great!
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Luger0815
noob


Registered: 12/21/10
Posts: 1,677
Loc: @ Home
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I'd like to answer, but im not a pro....
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Primz
Stranger
Registered: 05/15/11
Posts: 26
Last seen: 12 years, 7 months
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Hi all thats for the replys, just incase you didnt notice i am a complete novice so thanks for going gental
A liquid culture vial, i belive this is a vial where you can germinate your spores. (http://www.magic-mushrooms-shop.com/en/liquid-culture-vial.html) so i have a 10ml spore syringe and a 10ml vial.
im not sure what PC`d means but will look at FAQs again lol.
If i had 10p for every hour iv spent on thoes FAQs lol
Thanks in advance for any help people
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Wing
The Eye Tyrant



Registered: 04/25/11
Posts: 3,293
Last seen: 5 months, 11 days
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Re: In need of a Pro [Re: Primz]
#14456649 - 05/15/11 02:46 PM (12 years, 8 months ago) |
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PC = Pressure Cooked ... or sterilized
-------------------- My Old Grow Logs
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