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Invisiblespacytracie
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dextrose/l malt extract liquid culture question
    #14455793 - 05/15/11 11:40 AM (12 years, 8 months ago)

i have bought a packet of beer enchancer containing Spray Dried Malt Extract(Malted Barley,Water). Sparay-Dried Dextrose (Dextrose,Water), will this be sufficient 5o make a LC?


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OfflineBuckeye Oysters
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Re: dextrose/l malt extract liquid culture question [Re: spacytracie]
    #14455809 - 05/15/11 11:42 AM (12 years, 8 months ago)

yes, when buying malt make sure you get the lightest colored type, but all will work


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Evolution is Lamarckism in disguise.  Adaptation never creates a new species or trait, but rather the new species/trait always existed within the parent DNA until circumstances allowed it to be activated.  For instance, every wolf has the DNA for poodles, but that DNA would never be revealed without man selectively breeding for it.


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Re: dextrose/l malt extract liquid culture question [Re: spacytracie]
    #14455815 - 05/15/11 11:43 AM (12 years, 8 months ago)

Yes use 1 tbsp of ME and 1 tbsp of dextrose per quart of water


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Invisiblespacytracie
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Re: dextrose/l malt extract liquid culture question [Re: steelmonkey]
    #14455916 - 05/15/11 12:08 PM (12 years, 8 months ago)

i am using a ready made mix for beer fermentation that doesn't specify quantities yet states Spray Dried Malt Extract (Malted Barley, Water), Spray-Dried Dextrose (Dextrose, Water). I have the receipt so I can bring it back to the shop but was hoping i  can use this as is for a LC. I reckon i could use this this thinned out as a medium for a LC. Before I start,is this a good medium?


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Invisiblespacytracie
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Re: dextrose/l malt extract liquid culture question [Re: spacytracie]
    #14455922 - 05/15/11 12:10 PM (12 years, 8 months ago)

this has both miuxed in one packet


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Re: dextrose/l malt extract liquid culture question [Re: spacytracie]
    #14455938 - 05/15/11 12:14 PM (12 years, 8 months ago)

Well if you can take it back I would and get Light Malt extract (it will look like a light brown powder) and some dextrose(basically looks like icing sugar) both are cheap and they will have it if its a home brew store


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Invisiblespacytracie
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Re: dextrose/l malt extract liquid culture question [Re: steelmonkey]
    #14455986 - 05/15/11 12:24 PM (12 years, 8 months ago)

Damion5050's tek dsescribes a Light Malt Extract plus a Dextrose mix, the powder i got is both mixed together, can i simply use what i bought,seeing as it has the two (yet i don't know  quantities-this is for Bee4r  fermentation). I guess to use this stuff i bought within parameters of a dextrose |LC but wanted to ask advice first before getting into trouble and wasting weeks of timeo


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Re: dextrose/l malt extract liquid culture question [Re: spacytracie]
    #14456014 - 05/15/11 12:32 PM (12 years, 8 months ago)

Its perfectly fine to use myc will basically grow in anything with digestable sugars,the key is not to go too rich on the sugars


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Re: dextrose/l malt extract liquid culture question [Re: spacytracie]
    #14456017 - 05/15/11 12:33 PM (12 years, 8 months ago)

is it the geordie beerkit enhancer?
if it is you want to use 4g per 100ml of water
So you should have plenty in the bag :smile:


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Invisiblespacytracie
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Re: dextrose/l malt extract liquid culture question [Re: Rivaman]
    #14456043 - 05/15/11 12:41 PM (12 years, 8 months ago)

steelmonkey:i guessed this would be fine with sugars if not too strong though i am yet to make a succesful LC

Rivaman: You are right, thgis is exactly the Geordie Beerkit Enhancer!
What do you reckon?


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Re: dextrose/l malt extract liquid culture question [Re: Rivaman]
    #14456075 - 05/15/11 12:48 PM (12 years, 8 months ago)

I use it and it's great, just don't PC it for more than 20 mins (for a baby bottle) and you won't have any problem with it.


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Invisiblespacytracie
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Re: dextrose/l malt extract liquid culture question [Re: Rivaman]
    #14456098 - 05/15/11 12:54 PM (12 years, 8 months ago)

Ryaman: I am bewildered that you guessed the exact packet that i bought, how much should i use use per pint/or equivelent? Sorry to ask so many questions


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Re: dextrose/l malt extract liquid culture question [Re: Rivaman]
    #14456126 - 05/15/11 01:02 PM (12 years, 8 months ago)

You want to use 4 gram per 100 ml so 20 for 500 ml, probably 23 per pint,
I've not been using Lc for long but I'm not the only one to use Geordie, It's damn good stuff, and quite cheap, you should get about 45 pints out of that bag :thumbup:


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Invisiblespacytracie
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Re: dextrose/l malt extract liquid culture question [Re: Rivaman]
    #14456187 - 05/15/11 01:12 PM (12 years, 8 months ago)

Thanks so much me old mate, this is a great help - all the best !


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Re: dextrose/l malt extract liquid culture question [Re: spacytracie]
    #14456194 - 05/15/11 01:13 PM (12 years, 8 months ago)

Quote:

spacytracie said:
Ryaman: I am bewildered that you guessed the exact packet that i bought





Psikick i suppose :wink:


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Re: dextrose/l malt extract liquid culture question [Re: Rivaman]
    #14457366 - 05/15/11 04:35 PM (12 years, 8 months ago)

id pc for 45min, not twenty.  that is standard time for agar / malt blends


--------------------
Evolution is Lamarckism in disguise.  Adaptation never creates a new species or trait, but rather the new species/trait always existed within the parent DNA until circumstances allowed it to be activated.  For instance, every wolf has the DNA for poodles, but that DNA would never be revealed without man selectively breeding for it.


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Re: dextrose/l malt extract liquid culture question [Re: Buckeye Oysters]
    #14457404 - 05/15/11 04:42 PM (12 years, 8 months ago)

No offence but I disagree 15 mininum but I never go over 30 It's not needed on LC's you just end up with caramlization and sediments.


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Re: dextrose/l malt extract liquid culture question [Re: steelmonkey]
    #14459431 - 05/15/11 10:04 PM (12 years, 8 months ago)

Light carmelization doesnt hurt.  15min is prob only good for very small amounts like 100ml.  You always get sediments with yeast added if thats what you are thinking.  I can tell you that my bottle of agar says 45min, and agar, LC pretty much the same volume and sterilization requirements.  Lab grade Agar is cleaner than malt.


--------------------
Evolution is Lamarckism in disguise.  Adaptation never creates a new species or trait, but rather the new species/trait always existed within the parent DNA until circumstances allowed it to be activated.  For instance, every wolf has the DNA for poodles, but that DNA would never be revealed without man selectively breeding for it.


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Re: dextrose/l malt extract liquid culture question [Re: Buckeye Oysters]
    #14459516 - 05/15/11 10:20 PM (12 years, 8 months ago)

Well after five years and I don't know how many LC's PCing them for 20-30 tops your not likely to change my mind...why don't you poll and see how long people PC their LC's?


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Re: dextrose/l malt extract liquid culture question [Re: steelmonkey]
    #14460640 - 05/16/11 04:08 AM (12 years, 8 months ago)

I've only ever used the geordie kit and with the few Lc's I've done (probably about 10-12) I've noticed that if I'm stoned and i forget the PC is on and leave them in for more than 20 min's I get a LOT of large chunks of sediment and the liquid is a lot lighter than it should be, every time this has happened (3 or 4) the LC has failed.

So now, at least with 250ml bb lc's I set my phone alarm for 20 mins, and I've been thinking of dropping to 15, I'm sure that with the extra agitation of liquid boiling it doesn't need as long as a static substrate, and I've not had a contamination issue just a problem with burning up nutrients.


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Re: dextrose/l malt extract liquid culture question [Re: Rivaman]
    #14461142 - 05/16/11 08:55 AM (12 years, 8 months ago)

Exactly,if 45 minutes is long enough tp sterilize a dense mass like a cake it stands to reason a liquid wouldn't need 45 minutes.I've taken the lid off before the Pc is cooled and LC is still boiling in the jars


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Re: dextrose/l malt extract liquid culture question [Re: steelmonkey]
    #14461202 - 05/16/11 09:24 AM (12 years, 8 months ago)

Quote:

steelmonkey said:
Exactly,if 45 minutes is long enough tp sterilize a dense mass like a cake it stands to reason a liquid wouldn't need 45 minutes.I've taken the lid off before the Pc is cooled and LC is still boiling in the jars




:thumbup:


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Invisiblespacytracie
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Re: dextrose/l malt extract liquid culture question [Re: Rivaman]
    #14466296 - 05/17/11 04:49 AM (12 years, 8 months ago)

so far from what i've read,i'll try 20 minutes. i was wondering is it a good/bad idea to use more than 250cc's,like 500? or is that too much??


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Re: dextrose/l malt extract liquid culture question [Re: spacytracie]
    #14467234 - 05/17/11 10:27 AM (12 years, 8 months ago)

I make mine according to quart quantities in a big pot then strain through in coffee filters then pour into pint jars half full or a bit more never going ove three quarters full,which amounts to roughly 250 ml's (I'm assuming you meant ml instead of cc's).I wouldn'y make them bigger than that incase they contaminate.I highly recomend GLC instead of MS


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Invisiblespacytracie
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Re: dextrose/l malt extract liquid culture question [Re: steelmonkey]
    #14472007 - 05/18/11 07:30 AM (12 years, 8 months ago)

Ok, thank you. Does MS stand for Multi Spore?


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Re: dextrose/l malt extract liquid culture question [Re: spacytracie]
    #14472028 - 05/18/11 07:41 AM (12 years, 8 months ago)

Yes it does,good guess


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Invisiblespacytracie
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Re: dextrose/l malt extract liquid culture question [Re: steelmonkey]
    #14472123 - 05/18/11 08:29 AM (12 years, 8 months ago)

I was thinking, instead of de-pressurizing the jars before innoculating, would it be a good idea to simply give the jar lid a loosen and cover it in foil before pc'ing - then tighten it when the cooker has cooled?


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Re: dextrose/l malt extract liquid culture question [Re: spacytracie]
    #14472253 - 05/18/11 09:22 AM (12 years, 8 months ago)

What are you using for lids?(gas exchange)You definately don't want the lids tightened,just loose with foil is great


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Invisiblespacytracie
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Re: dextrose/l malt extract liquid culture question [Re: steelmonkey]
    #14477404 - 05/19/11 08:30 AM (12 years, 8 months ago)

i didn't kinow i had to have gas exchange for a Liquid Culture - i thought it was meant to be injection port only , am I wrong? My injection ports are 1/4 inch clear silicone without gas exchange holes, normal jar lids.
thanks for the advice so far


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Re: dextrose/l malt extract liquid culture question [Re: spacytracie]
    #14477423 - 05/19/11 08:37 AM (12 years, 8 months ago)

You could get away with out it but eventually it will stall out so it depends on if you want to just get enough to noc up a rye bag or whatever or you want a healthy lasting LC that you might want to propagate into more LC


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Re: dextrose/l malt extract liquid culture question [Re: steelmonkey]
    #14477441 - 05/19/11 08:44 AM (12 years, 8 months ago)

I've just been reading some related posts and sounds like air/gas exchange is the way to go so i'll make sure to have a ventilation hole covered with micropore. Thank you for leading me to9 the correct conclusions!


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Re: dextrose/l malt extract liquid culture question [Re: spacytracie]
    #14477477 - 05/19/11 08:55 AM (12 years, 8 months ago)

Micropore tape will work great that way if you do feel they are stalling out you can just peel back the tape under sterile conditions and give it some air.

I'm very glad I could help you out in any way :thumbup:


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