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PhishXIII
Stranger

Registered: 04/30/11
Posts: 63
Last seen: 3 years, 8 months
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Can you over-inoculate?
#14450796 - 05/14/11 11:45 AM (12 years, 8 months ago) |
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I ask because when I was inoculating, something happened to sort of "clog up" the needle. It would still inject with a bit of pressure though, but during one jar it broke free and I pumped like a full CC into just the one hole.
9 days later, that one jar I did that to is WAY ahead of my other jars in terms of mycelium spread.
So if I wanted to pump 4 CCs into each half pint in the future, would that be ok?
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Wing
The Eye Tyrant



Registered: 04/25/11
Posts: 3,293
Last seen: 5 months, 11 days
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Re: Can you over-inoculate? [Re: PhishXIII]
#14450805 - 05/14/11 11:46 AM (12 years, 8 months ago) |
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You can run the risk of over saturating your substrate
-------------------- My Old Grow Logs
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LayinUp
Crush yo rating like yo butthole



Registered: 04/14/11
Posts: 2,232
Loc: Between the permafrost an...
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Re: Can you over-inoculate? [Re: Wing]
#14450814 - 05/14/11 11:47 AM (12 years, 8 months ago) |
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Quote:
winged_1 said: You can run the risk of over saturating your substrate
^
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Escape the box.
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k00laid
NEMO


Registered: 05/03/10
Posts: 19,636
Last seen: 6 months, 5 days
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Re: Can you over-inoculate? [Re: LayinUp]
#14450867 - 05/14/11 11:55 AM (12 years, 8 months ago) |
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OP never even told us what he is pumping into these jars.....
posts like these i just dont understand..
i feel like these are all trolls...
-------------------- AMU - AMU Q & A - MyVideo Teks!
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avaldez314
Silent Bob



Registered: 04/26/10
Posts: 136
Last seen: 9 years, 10 months
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Re: Can you over-inoculate? [Re: PhishXIII]
#14450935 - 05/14/11 12:06 PM (12 years, 8 months ago) |
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I usually do 5 inoculations (*) per jar, 4 in the sides of the jar and 1 in the center. That speeds up the colonization process, at least it works for me :-)
I also tried to do 10 inoculations per jar (hey, it's free!), but I didn't noticed any significant improvement.
(*) from a spore syringe or from a liquid culture.
-------------------- Sclerotia producing print WANTED! P. Mexicana/A, P. galindoi/ATL#7 or P. tampanensis if you are interested in a trade please PM me!
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PhishXIII
Stranger

Registered: 04/30/11
Posts: 63
Last seen: 3 years, 8 months
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Re: Can you over-inoculate? [Re: k00laid]
#14450987 - 05/14/11 12:14 PM (12 years, 8 months ago) |
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Quote:
k00laid said: OP never even told us what he is pumping into these jars.....
posts like these i just dont understand..
i feel like these are all trolls...

Didn't realize the strain made a difference.
B+ and Thai Koh Samui. Using PF Tek.
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Bom_Tombadil
ShRoOm GrOwEr & EaTeR


Registered: 05/12/09
Posts: 206
Last seen: 8 years, 6 months
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Re: Can you over-inoculate? [Re: avaldez314]
#14450995 - 05/14/11 12:16 PM (12 years, 8 months ago) |
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i put 5 cc's of liquid culture into all my wbs jars i dont know if that helps or not
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k00laid
NEMO


Registered: 05/03/10
Posts: 19,636
Last seen: 6 months, 5 days
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Re: Can you over-inoculate? [Re: PhishXIII]
#14451029 - 05/14/11 12:21 PM (12 years, 8 months ago) |
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Quote:
PhishXIII said:
Quote:
k00laid said: OP never even told us what he is pumping into these jars.....
posts like these i just dont understand..
i feel like these are all trolls...

Didn't realize the strain made a difference.
B+ and Thai Koh Samui. Using PF Tek.
the name on the SPORE SYRINGE does not make a difference
it being a LIQUID CULTURE syringe could make a difference.
-------------------- AMU - AMU Q & A - MyVideo Teks!
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k00laid
NEMO


Registered: 05/03/10
Posts: 19,636
Last seen: 6 months, 5 days
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Re: Can you over-inoculate? [Re: k00laid]
#14451035 - 05/14/11 12:22 PM (12 years, 8 months ago) |
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but to answer your question 4CCS of liquid is unlikely to mess up a BRF cake
and it would have zero affect on a grain jar.
-------------------- AMU - AMU Q & A - MyVideo Teks!
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