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OoBYCoO
One grow down, a million to go!!



Registered: 08/18/10
Posts: 8,120
Loc: USA
Last seen: 10 years, 4 days
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Re: How do I put the needle on the syringe??? [Re: slapphappypill]
#14448506 - 05/13/11 10:36 PM (12 years, 8 months ago) |
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dylanSnow
Learner


Registered: 03/22/11
Posts: 19
Loc: Pennsylvania
Last seen: 2 years, 4 months
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Re: How do I put the needle on the syringe??? [Re: OoBYCoO]
#14448658 - 05/13/11 11:13 PM (12 years, 8 months ago) |
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Don't let 'em give you shit (as funny as it is.)
I had a hard time with my first syringe too.
I just used my teeth to get it off and on. (Joking, DO NOT do that. Along with a mouth stab, you'll contaminate your entire syringe.)
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orange771
Registered: 01/26/11
Posts: 288
Last seen: 1 year, 7 months
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Re: How do I put the needle on the syringe??? [Re: dylanSnow]
#14448690 - 05/13/11 11:22 PM (12 years, 8 months ago) |
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When I got my very first syringes ever, I showed my wife and she immediately pulled off the cap on one syringe, risking contamination. I should have smacked her silly (jk). But hey man she did it without thinking, I'm glad you figured it out! Haha
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maug



Registered: 11/28/10
Posts: 1,703
Loc: inside you
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Re: How do I put the needle on the syringe??? [Re: orange771]
#14448846 - 05/13/11 11:56 PM (12 years, 8 months ago) |
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I had a problem with my first syringe too.
What I do now: Put a piece of foam and the needle in a lid with isopropyl alcohol. Twist out the plug, put on the needle. I have those same syringes, and don't worry - there's a small trap near the top to avoid contamination. Flame the needle, and the alcohol lights up. Give it a little squeeze to flush out the trap while it's being flamed. The alcohol in the needle should evaporate. Stick it half-way in the foam/sponge while it's red hot. Stick it through to inoculate. Leave the needle hanging until you can patch it with masking tape and pull out the needle in one motion. No need for the glove box or flow hood. When you are done, you can cap the needle by dipping it in candle wax.
I would recommend making a liquid culture with some of that, if you haven't already.
cheers.
-------------------- I think nighttime is dark so you can imagine your fears with less distraction. -Calvin and Hobbes
Edited by maug (05/14/11 12:01 AM)
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OoBYCoO
One grow down, a million to go!!



Registered: 08/18/10
Posts: 8,120
Loc: USA
Last seen: 10 years, 4 days
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Re: How do I put the needle on the syringe??? [Re: maug]
#14448874 - 05/14/11 12:02 AM (12 years, 8 months ago) |
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Quote:
maug said: I had a problem with my first syringe too.
What I do now: Put a piece of foam and the needle in a lid with isopropyl alcohol. Twist out the plug, put on the needle. I have those same syringes, and don't worry - there's a small trap near the top to avoid contamination. Flame the needle, and the alcohol lights up. Give it a little squeeze to flush out the trap while it's being flamed. The alcohol in the needle should evaporate. Stick it half-way in the foam/sponge while it's red hot. Stick it through to inoculate. Leave the needle hanging until you can patch it with masking tape in one motion. No need for the glove box or flow hood. When you are done, you can cap the needle by dipping it in candle wax.
I would recommend making a liquid culture with some of that, if you haven't already.
cheers.
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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Re: How do I put the needle on the syringe??? [Re: OoBYCoO]
#14448889 - 05/14/11 12:06 AM (12 years, 8 months ago) |
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There is no such thing as a stupid question, though this one did come close. 
Glad that you got it worked out.
-------------------- Stop cold shocking your mycelium! Hot Spawn - Get It On 
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occollegeboi
MushroomSpaceGod



Registered: 04/10/11
Posts: 2,857
Last seen: 5 years, 4 months
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Re: How do I put the needle on the syringe??? [Re: argg]
#14448947 - 05/14/11 12:20 AM (12 years, 8 months ago) |
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Quote:
argg said: just don't put the needle part down on a dirty table like the pic. the inside is exposed to nasties that way.
Do you think there would be any contams if I used a bleach wipe prior to setting down anything? I cleaned the whole table with lemon scented bleach wipes.
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Re: How do I put the needle on the syringe??? [Re: occollegeboi]
#14448964 - 05/14/11 12:25 AM (12 years, 8 months ago) |
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Not sure, I use 91% iso alcohol/oust
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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ShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~




Registered: 07/18/10
Posts: 8,139
Loc: EVERY WHERE
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Re: How do I put the needle on the syringe??? [Re: slapphappypill]
#14449035 - 05/14/11 12:44 AM (12 years, 8 months ago) |
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maug



Registered: 11/28/10
Posts: 1,703
Loc: inside you
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Re: How do I put the needle on the syringe??? [Re: OoBYCoO]
#14449143 - 05/14/11 01:21 AM (12 years, 8 months ago) |
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Quote:
OoBYCoO said:
Quote:
maug said: I had a problem with my first syringe too.
What I do now: <snip>

Is that bad?
-------------------- I think nighttime is dark so you can imagine your fears with less distraction. -Calvin and Hobbes
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OoBYCoO
One grow down, a million to go!!



Registered: 08/18/10
Posts: 8,120
Loc: USA
Last seen: 10 years, 4 days
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Re: How do I put the needle on the syringe??? [Re: maug]
#14449167 - 05/14/11 01:28 AM (12 years, 8 months ago) |
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When you're dipping your needle in candle wax, I'd say yes.
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OoBYCoO
One grow down, a million to go!!



Registered: 08/18/10
Posts: 8,120
Loc: USA
Last seen: 10 years, 4 days
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Re: How do I put the needle on the syringe??? [Re: OoBYCoO]
#14449178 - 05/14/11 01:31 AM (12 years, 8 months ago) |
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Each needle comes w/ it's own cap... why would you want to dip it in wax?
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maug



Registered: 11/28/10
Posts: 1,703
Loc: inside you
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Re: How do I put the needle on the syringe??? [Re: OoBYCoO]
#14449201 - 05/14/11 01:37 AM (12 years, 8 months ago) |
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Well, I did that for the first time the last time I inoculated, and I'm going to inoculate tonight with the same syringe. I was thinking that it would just melt off, or pop off. I guess I'll find out soon enough if it's going to be a problem, but I've used wax as an air-tight seal when making jam.
-------------------- I think nighttime is dark so you can imagine your fears with less distraction. -Calvin and Hobbes
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Re: How do I put the needle on the syringe??? [Re: maug]
#14449213 - 05/14/11 01:41 AM (12 years, 8 months ago) |
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Quote:
maug said: Well, I did that for the first time the last time I inoculated, and I'm going to inoculate tonight with the same syringe. I was thinking that it would just melt off, or pop off. I guess I'll find out soon enough if it's going to be a problem, but I've used wax as an air-tight seal when making jam.

We aint making jam... cultivating mushrooms is a little different
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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maug



Registered: 11/28/10
Posts: 1,703
Loc: inside you
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Re: How do I put the needle on the syringe??? [Re: slapphappypill]
#14449509 - 05/14/11 03:12 AM (12 years, 8 months ago) |
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Quote:
slapphappypill said:
Quote:
maug said: Well, I did that for the first time the last time I inoculated, and I'm going to inoculate tonight with the same syringe. I was thinking that it would just melt off, or pop off. I guess I'll find out soon enough if it's going to be a problem, but I've used wax as an air-tight seal when making jam.

We aint making jam... cultivating mushrooms is a little different
The reason for the air tight wax seal with jams is to fight mold growth. It is effective enough for not allowing air circulation.
The wax is not clogging up the inside or anything. It's a single drop of wax on the slanted sharp point. It's going to melt right off. I'm not using a flow hood or glove box, but there's a little salt in my LC's too. Please explain with a little more detail how this going to ruin my mushroom cultures... Maybe I'm not understanding your point.
This is off topic from the original post, and It's not an issue as far as I know. You may PM me if you'd like. I do wanna welcome any advice.
-------------------- I think nighttime is dark so you can imagine your fears with less distraction. -Calvin and Hobbes
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dylanSnow
Learner


Registered: 03/22/11
Posts: 19
Loc: Pennsylvania
Last seen: 2 years, 4 months
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Re: How do I put the needle on the syringe??? [Re: maug]
#14450280 - 05/14/11 09:44 AM (12 years, 8 months ago) |
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I don't think it will hurt anything. It just seems abit overkill. Most syringes come with a cap. I wipe or dip the cap in alcohol (dry it or let it evaporate) then put the cap back on.
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Magicman69
All About the Benjamins



Registered: 05/29/13
Posts: 6,876
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Re: How do I put the needle on the syringe??? [Re: awakendone]
#18363464 - 06/03/13 04:47 PM (10 years, 7 months ago) |
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I was wondering the same thing. I'm pretty new to the hobby, but it seems like the minute I take the plug out to screw on the needle i'll expose all the spores to contaminated air. Potentially contaminating the entire syringe. Paranoia or relatively possible?
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