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OfflineBiostatik
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Long time lurker, first grow log and introduction.
    #14424875 - 05/09/11 02:18 PM (12 years, 9 months ago)

Greeting everyone! Call me Statik. I've been lurking for a while learning as much as I can from all the great information found via the search function.:tongue: I've been a member of several different forums, so I know the drill, and wont contribute to unnecessary topic posting. (thank gawd!:rolleyes: I like him already!)

I dont have much to post right now except for my still in progress grow log, which I decided to post due to its adoption of various techniques, and applications for people who live in colder climates like myself. (What I mean by that is many people tell OP's they need not incubate, but many people live in cold climates and it's fairly necessary to achieve growth in a month or so. results vary of course). It also features a well rounded experience, with variations of techniques, and anomalies, strains, and container construction. I just feel it might offer some ideas if nothing else. Maybe not. In either case, I hope it's a fair contribution.

The grow log is about a week and a half in, and I will update it as periodically.

First Grow Log

Intro:

After spending much time researching and reading through the forums and grow teks I finally started my first grow. I chose my spores without researching specific strains and selected Golden Teachers as my first strain. This purchase was made from the sponsor Lil Shop of Spores. I gathered my materials and set up my incubator and fruiting chamber and tested them out to ensure I could sustain the desired conditions over a period of time. I needed to experiment a little bit to achieve this goal.

My grow is based off of the PF Tek, with several modifications and adaptations to suit my own needs and conditions, and utilizing concepts from other grow teks, plugged in where it made sense.

My first order of Golden Teachers came in, and the vendor included a bonus syringe of Mexi-Cyan. A week after I inoculated the first batch I received my second order of Puerto Rican, and again, the vendor included a bonus syringe of Thai Koh Samui. I inoculated the second batch that night.


Incubator:

Single tote-Clear-Covered.
(Sterilized with a bleach/water mix before and after tinfoil)

(All hardware sterilized in the same manner)
Interior is lined with tinfoil, on 5 sides, the bottom of the lid is not lined. The lid does however have tin foil on the top side, with a 1"x2" square removed on one of the corners. This functions as a window for looking inside to view the digital thermometer/hygrometer placed beneath the window without opening the tub to contams. I have placed a medium sized terrarium heat rock in the center of the tote to keep the heat in the tote up. Additionally a tall cylindrical tupperware container full of water was placed in the incubator, with a 10 gallon fishtank heater submerged in the water. Lastly a tube for an air pump with an air stone attached bubbled the water, the vision of which was to keep a warm humid climate.


Fruiting Chamber:

Single tote-Clear-Covered.
(Sterilized with a bleach/water mix)

(All hardware sterilized in the same manner)
2" of wet perlite at the bottom. Minimal water pooled. Heat rock will be placed again in the center of the chamber. Tupperware container filled with water and 10 gallon fishtank heater. Filtered air pump and air stone to agitate water surface. 1/2"-1" above perlite layer, I have drilled holes every 2" around the bottom, and no where else. The goal being complete automation. The theory is the air pump will force filtered air through the air stone, into the the water. This should agitate the water, which will be heated by the fish tank heater. This will add warms to the chamber, as well as humidity, compensating for the lack of standing water under the perlite, and the lower amount of perlite in general.

Additionally, the air pump SHOULD make the internal pressure of the chamber higher than the outside pressure, meaning air will try to escape the chamber through any openings. This is where the holes at the bottom of the chamber come into play. I figure, since CO2 is heavier than air, and it sits at the bottom of the tank, then the air that is pumped into the chamber will go up, and as the internal pressure increases, the air will push downward on the CO2, forcing it out of the holes at the bottom of the chamber. The steady flow should keep contams from coming in the holes, as well as allowing air exchange without opening the chamber. A florescent light will be on a timer, as well as the air pump and heaters if needed.


Grow Room:

A small closet upstairs, emptied and cleaned thoroughly with a bleach/water spray. Door is kept closed and locked at all time unless I am in there.


Procedures-Batch one:
(Golden Teachers and Mexi-Cyan)

Substrate Prep-
-Vermiculite
-Organic Brown Rice Flour
-Tap water
-7 Pint sized Jars

I did not use very strict sterile procedures here. I washed the jars in bleach water and Dawn dish soap, as well as my hands. I did not wash the bowls (whoops). I drilled 4 1/4" holes in each of the lids before washing. I used the recipe from the "Lets Grow Mushrooms" video. Some jars I did not fill enough, resulting in a dry verm layer of about 1" (another whoops). Before adding the dry verm, I wiped the remaining jar walls with a dry paper towel then added the dry verm to the top. I put the lids on seal side up immediately after filling each jar, then a tin foil square over each jar. I put them all in a clam steamer with a lid, and steamed them for 1 and 1/2 hours.

When the steaming was complete, I let them cool with the lid off until I could safely touch them and then I placed them all on a clean counter top. I used a fan, which I cleaned with a bleach/water mix, to cool them off faster. This took about 30 minutes, but I let them cool another 30 minutes.


Inoculation: (28 April-Thursday Night)

When they were cool, I prepared my inoculation area. I used a clean counter top, laid paper towels, and then laid out my syringes and other materials. I started with the Golden Teachers and removed the tin foil from each jar one at a time for inoculation. I believe I used more fluid than needed, as the 10cc syringe lasted about 3 and 1/2 jars. I flame sterilized the needle and rubbed it down with an alcohol wipe between jars. Immediately after injecting in all 4 holes I replaced the tin foil cover. The last bit of Golden Teacher fluid, went into a jar that later I finished with the Mexi-Cyan. I used the Mexi-Cyan more sparingly in the rest of the jars, but I still only managed to inoculate a total of 7 jars (one 50/50). I identified the Mexi-Cyans with a small piece of masking tape at the bottom of the jar. I did nothing to sterilized it :crazy:. The dual strain jar was marked with two pieces of tape. I brought the jars up to the grow room, and placed them in the incubation chamber after removing the tin foil covers.

A few days into the incubation, I noted that the RH was at 99%. I opened the chamber and fanned out the air to disperse the humidity until the hygrometer read 77% and then I turned off the air pump. I replaced the lid and waited until the next day to see how the RH level had been effected. After 12 hours it had reached 99% again. I removed the container of water, as well as the heater and air tube. From that point on the temp and RH levels have remained stable and ideal.

Stats so far: (Batch 1)
Inoculation-28 April (Thursday Night)
First Signs of growth-1 May (Sunday Morning)
Time elapsed-2.5 days
Average Temp-78-84 degrees
Average RH-80-85%

After one week (5 May, Thursday night) colonization had reached about 20-25% in most of the jars. Some injection sites had been much further ahead than others in the same jar. Two of my jars had very little growth, and I noted that these were the jars with too much of a dry verm layer. Some myc had even started to grow in the dry verm layer to my surprise. That small growth eventually saved the jars as the myc grew down to the BRF cakes, none the less, still behind. By 8 May (Noon Sunday) all 6 jars had reached 40-55% colonization. A sudden and speedy leap, I think, but perhaps it is normal. That same day I also noticed two jars had wet spots, about the size of a quarter in one, and about an 1"on the other one. There is no color to it, simply appears like a spot of cake wetter than the surrounding. Did some reading and census says it could be myc piss, a contam, or nothing. I will wait and see what happens. Also one jar has a small rust colored spot. It has not grown since I noticed it, but I will keep an eye on it.

Reading and researching during the whole process, I happened on a few threads discussing CO2 buildup inside the jars potentially causing slowed growth. The opinions were about 50/50 so, unsure about whether or not CO2 build up was a legitimate concern, I turned all 7 jars upside down in hopes that the CO2 would drain out. I turned them back up right the next morning, 9 May. Later that day I had not noticed much improvement, not that I expected them to kick into high gear, but just noting. The jars with the wet spots had mycellium grown up to the spots borders. All jars are currently at 50-60%.

On 11 May, one day short of 2 weeks in, Batch one was just about 75% colonized across the board except for one. The jar with the rust spot is doing well, and the spot has not grown or changed at all, so I suspect it actually IS rust. From what I've read, contams are typically aggressive and grow quickly, and we're 3 days with no grow now. One of the jars with the 'wet spot' continued to colonized past its spot, but the other had not. In fact it appeared to have stalled completely at about 40-45%. This was the dual strain jar. Still unsure about what it was, I took it out for closer examination. With careful study, suspected that it wasn’t a wet spot at all, perhaps a "loose" spot. There appeared to be depth there, as if that portion of the cake was not solid. I gave the jar a few jarring shakes, and hit it a few times on my palm. The cake loosened around that spot revealing more depth, as if it was an air bubble that had got trapped inside the cake. It was the weirdest thing. Positive the myc would grow no further in this situation I decided to birth it early. I removed the lid, dumped out the dry verm, then put the lid back on, to loosen the cake by hitting it on a sturdy box. When loose, I pulled it out of the jar over the trash can. Perhaps not the best idea in hind sight, but unfortunately for me I was in a hurry, and needed to move. I cleaned it off in filtered tap water, submerged it and put it in the fridge. I am interested to see if one strain prevailed, or if I will get two strains growing off the same cake. Maybe they will merge as one? I don’t really know, I think I'll read about that.

I did not have time to set up my fruiting chamber to have the climate stable by the next day. I will do that tonight, and the cake will have to deal with some fluctuations. I will probably take the heat rock from the incubator and put it in the fruiting chamber as popular vote says it's too warm in my incubator anyway. I’m not that worried, the incubator has been very good at keeping a steady climate, and the jars should be ready for birthing in a few days. If the temp fluctuates deviates too much I will go and buy another heat rock for the sake of being consistent in experimentation. I don’t want to change too much mid cycle.

......................................................................


Procedures-Batch 2:
(Puerto Rico and Thai Koh Samui)

Substrate Prep-
-Vermiculite
-Organic Brown Rice Flour
-Tap water
-10 8oz Jars

Same process as before except I sterilized the jars in the dishwasher, as well as both bowls. Also I was more careful to fill the jars more so as to only leave 1/2" of jar wall to correct the mistake I made in the first batch. I ran out of substrate on the last jar and only managed to fill it about half way. I put the dry verm layer in and treated it the same as the others. This time I wiped the wall and rim down with an alcohol wipe before adding the verm layer. Also rather than drilling holes in the jar lids, I used tin foil as a lid, poke 4 holes near the edge, and a second tin foil layer over that (my drill broke). I steamed the jars in the same manner as batch one, and cooled them in the same manner as well.


Inoculation: (5 May-Thursday Night)
Same process as before. Used the inoculation fluid MUCH more sparingly, further complemented by the smaller jar size. STILL I only managed to knock up 10 jars with two 10cc syringes. One jar at a time, I removed the top tin foil layer, injected in each hole, and replaced the tin foil layer immediately. The jar that was only half full I needed to remove the tin foil lid completely to get deep enough to inject. I was very careful with this one. Marked each Koh Samui with masking tape in the same manner as I identified the Mexi-Cyans, but since they are smaller jars, there will be no confusion. I also made another dual strain, and marked it with two pieces of tape, just like I did with the dual strain in batch one. After all the jars were injected, I brought them up to the grow room,
removed the top tin foil layer and put them in the incubator with the first batch. By this time I had already removed the water container from the chamber, and the temp and RH had stabilized. I noticed the first signs of growth 2.5 days later, as spotty myc in the Puerto Rican jars only.

Stats so far: (Batch 2)
Inoculation-5 April (Thursday Night)
First Signs of growth-8 May (Noon Sunday)
Time elapsed-2.5 days
Average Temp-78-84 degrees
Average RH-80-85%

On May 9th 3pm I noticed the Puerto Rican jars had displayed an aggressive jump in growth. From mere signs of spotty life to 25% in less than 24 hours. The Koh Samui also shows signs of life now, though I think I could have spotted them earlier this morning, but I was only concerned with flipping the other jars up right. So they are a bit further ahead than "signs of life."

11 May (Day6) The jars have disappointed me slightly. I expected these smaller jars to colonize faster. I took more care in preparing them, and even used a generous amount of spore solution, not too much, but generous. They are certainly progressing, they just aren't living up to my expectations. The pints went much faster. Anyway, I became frustrated with having to remove these small jars to get a look at them due to the tin foil covering the sides. I decided to drill the jar lids and put them on. I did this, and cleaned each lid with alcohol, and carefully and cleanly applied the lids to each jar. I predict them to be 100% colonized in just under a week. And at this time, no signs of contams or problems at all. 
......................................................................


--------------------
I use the search function!
*bows* "thank you, thank you".

Edited by Biostatik (05/12/11 06:32 AM)

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Offlinebateyes88
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Re: Long time lurker, first grow log and introduction. [Re: Biostatik]
    #14424956 - 05/09/11 02:35 PM (12 years, 9 months ago)

Welcome to the forums. This is a great place, as you've prob already figured out. You have a knack for tek/log writing. That will serve you well if you ever need to reverse engineer a problem.
Do you have any specific questions or worries? It looks to me like your on the right track. Kudos on doing your homework before wasting a bunch of money. Trail and error is a valid way to learn- but gets expensive!

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OfflineBiostatik
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Re: Long time lurker, first grow log and introduction. [Re: bateyes88]
    #14425018 - 05/09/11 02:51 PM (12 years, 9 months ago)

Thanks man. I get just as irritated when people ask things over and over and over. however it does give the new guys a chance to answer questions and contribute. I dont have any questions just yet, except for the rust spot, which my research turned up with mixed answers. Also the we spots, I could not find a solid answer for. Both the wet spots and the rust colored spot could either be a contam, or exactly what they look like. I'm just waiting to see if they grow.

Also Im looking forward to seeing if my fruiting chamber design will work well. I'm really hoping that my internal pressure pushing the CO2 out the bottom holes design will function as planned. I'm also worried that the bubbler will make the chamber too humid like it did in the incubator, but with the air flow I might be okay. Nothin to it but to wait and see. :tongue:


--------------------
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InvisibleFrankHorrigan
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Posts: 10,573
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Re: Long time lurker, first grow log and introduction. [Re: Biostatik]
    #14425032 - 05/09/11 02:55 PM (12 years, 9 months ago)

Hello statik!

Oh boy, you've been following some outdated / bad advice.  At the risk of sounding like an ass (cause you are doing good) I'm gonna correct your errors I see.

Flipping jars is a terrible idea.  There's plenty of gas exchange in the jars with open inoc holes. All flipping does is "break" the dry verm barrier and let in any contams it was protecting your substrate from.  Also, myc needs high levels of CO2 to colonize at an ideal rate.

Ditch your incubator.  Colonization is faster at room temp (70-75) and way less prone to contams :shocked:  Also, standing, heated water is a heaven for contams to grow and take hold in.  RH and all that are conditions you need to worry about for fruiting, not for colonizing.

Your wet spots are most likely due to too much spore solution being injected.  Ideally you can use 1/4 - 1/2cc per hole, or 1cc per jar.  Are the wet spots near your inoc holes?  If not, it may be contam, see what happens when the myc gets to it.

Pint jars are going to give you poor performance.  Too late now, they will work if they don't stall out colonizing.  You're fighting time and gravity, as the longer it takes, the more compact your BRF/verm substrate becomes, and the harder it is for the myc to move in.  $20 for 24 half pints will save you loads of time and money :smile:

I know it seems like it won't work and it's very simple, but shotgun FCs really do work amazingly in all conditions.  Spend a couple hours with a drill bit and put holes in all 6 sides and return your heaters and stones and air pumps.  Trust me, SGFC is the way to go for cakes.

If you don't want to do that, get rid of the heat stone, and pooling water.  Perlite does not "wick" water up, your standing water is evaporating on its own and will be an easy access for contams.  Heating your terrarium is only going to lower your RH and create tons of condensation.

I hope this helps. I went through a long hard road of learning all of this the hard way, but I really believe that if you get rid of your incubator and build an SGFC your grow will be much simpler, easier and perform optimally.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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InvisibleFrankHorrigan
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Re: Long time lurker, first grow log and introduction. [Re: Biostatik]
    #14425087 - 05/09/11 03:05 PM (12 years, 9 months ago)

Quote:

Biostatik said:
Both the wet spots and the rust colored spot could either be a contam, or exactly what they look like.



Do you have any pics?
If it's a strange color that's growing, I'd assume contam.
Wet spots are hard to tell tho.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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InvisibleDaft Punk
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Re: Long time lurker, first grow log and introduction. [Re: FrankHorrigan]
    #14425099 - 05/09/11 03:07 PM (12 years, 9 months ago)

i second that motion for ditching the incubator. when myc grows it creates heat meaning that the core of the cake is hotter than the outside and the only possible way to accurately measure temp would be to have a thermometer stuck in each cake which is ridiculous. keep it in the low 70's and they will take care of themselves. you are just facing slower colonization times and possible contam otherwise.

sounds like you have a solid plan. follow the suggestions of yakwhacker and you will be on your way to success

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Offlinebateyes88
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Re: Long time lurker, first grow log and introduction. [Re: Daft Punk]
    #14425330 - 05/09/11 04:04 PM (12 years, 9 months ago)

I sometimes get a rust-colored spot when I use LCs, but not MSS. Is it growing at all?
Also, Im from the pro-incubator school. I dont want to turn your thread into another incub debate-theres been moren' enough- but I live in ME, much cooler temps- in a drafty old house. One thing Ive learned is: what works for me may not work for you. You'll see that over and over no matter what the topic. Since you have decided to use one, Ill lay this on ya. From what Ive seen, RH in the incubator isnt all that important FOR JARS. If you start using it for casings/trays, then you'll need to be more picky.
Shotguns are tried and trued FCs. Many have tweeked it, made hybrids- some have had good luck, most go back to shotguns original design. Try yours out n see how it goes. If its performance isnt to yer liking, try a SGFC. I loved mine, but have since switched to an "indoor greenhouse". If you get so you need the room, you might look into those. Ive got $60 into mine, and I wouldnt trade it for the world.
When I first started, I used an ave of 2cc per jar. I think thats a rookie thing. Its kinda hard to dispense 1/4cc at first. Youll get it. Its really all you need. Less is more- at least w/ MSS. LC is a lil diff story.(But one you'll soon wanna read. LCs RULE. Turn 1 syringe into 1,000)
Pint jars, unfortunately, do suck. Usually. I have a dozen from when the store was out of 1/2s. Its very hard to get em to fully colonize. When they start to look dry, and the shrink up so the verm layer falls- birth em, cut off the un-colonized parts- and DnR as normal. I only use em as last resort and for LCs now. I think Ive had 3 out of, say, 24 grows reach 100% colonization w/ em.

I think you'll figure it out. Its obvious you've done yer homework, and have a brain. You keep good notes. Thats important. When trying different things during the same grow, Ive found it helps to number the jars. Maybe Im anal, but it helps when looking back and determining what works and what doesn't. You'll do fine. If you need help, or prints etc, give a hollah.

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InvisibleFrankHorrigan
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Re: Long time lurker, first grow log and introduction. [Re: bateyes88]
    #14425380 - 05/09/11 04:12 PM (12 years, 9 months ago)

Quote:

bateyes88 said:
I live in ME, much cooler temps- in a drafty old house.



I still doubt your temp dips below 65 that often, and it's spring time to boot...
Ditch the incubator, buy a radiant type (oil-filled) space heater and warm your ambient temp up in your grow room.
For FCs, dewpoint becomes an issue and RH tops out lower if an FC is heated.
Of course it'll still work, cubes are easy, and assuming no contams take hold, but why go through all the trouble and risk?


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Offlinebateyes88
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Re: Long time lurker, first grow log and introduction. [Re: FrankHorrigan]
    #14425500 - 05/09/11 04:39 PM (12 years, 9 months ago)

I dont heat martha. Just the GC/incubator... Im having awesome results. I cant complain when my PF cakes look like this:

Thats why I said: what works for you may not work for me...and vice versa. Damn. Ive tried 100 different things 100 different ways. Everyone has to find the best set-up for their individual climate/circumstances...

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OfflineBiostatik
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Re: Long time lurker, first grow log and introduction. [Re: bateyes88]
    #14434112 - 05/11/11 09:51 AM (12 years, 9 months ago)

damn bateyes those are some nice lookin cakes haha. well my jars are nearing complete colonization, most of the 1st batch anyways. I'll continue to heat these two batches and on the 3rd I will try without heat. It will be much warmer by then anyways. I will compare results when the time comes. Im very excited to see how this turns out. especially my pint jars. I wonder if pint jars will give bigger flushes...i havent read about that yet. i think i will.

thank you all for your tips and comments! If you have interest, stay tuned for my updates to the grow log, and see how this turned out! great to meet you all (sort of...online...eh.)


--------------------
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InvisibleFrankHorrigan
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Re: Long time lurker, first grow log and introduction. [Re: Biostatik]
    #14434465 - 05/11/11 11:33 AM (12 years, 9 months ago)

good luck OP.
Refer to my sig for RR's notes.
They will clear up a lot of conflicting info :shrug:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Offlinebateyes88
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Re: Long time lurker, first grow log and introduction. [Re: FrankHorrigan]
    #14435504 - 05/11/11 03:51 PM (12 years, 9 months ago)

They pints should give bigger extended flushes. Just hard to get em to fully colonize. Remember to soak the pints a few extra hrs when you DnR. If they dont fully colonize, and you end up cutting off un-colonized bits, you might consider a "crumble-cake" Tek. Just put the cake in a bag w/ a lil verm and water, close bag, crumble to no bigger than marble size pieces(I usually go smaller) and dump into a tupperware type container. You'll get bigger flushes thn with just cakes. Look at the pins in this monotub. This was just 2-3 1/2pint cakes crumbled w/ verm...

Click on Pic to see full pinset...

For sure, though. RRs vids are an awesome reference for noobs- or anyone. I just re-watched the grain ones a few nights ago. Hard to argue w/ his experience- both personal, and combined from being on here forever!

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OfflineBiostatik
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Re: Long time lurker, first grow log and introduction. [Re: bateyes88]
    #14438836 - 05/12/11 06:30 AM (12 years, 9 months ago)

Batch 1 Update:

On 11 May, one day short of 2 weeks in, Batch one was just about 75% colonized across the board except for one. The jar with the rust spot is doing well, and the spot has not grown or changed at all, so I suspect it actually IS rust. From what I've read, contams are typically aggressive and grow quickly, and we're 3 days with no grow now. One of the jars with the 'wet spot' continued to colonized past its spot, but the other had not. In fact it appeared to have stalled completely at about 40-45%. This was the dual strain jar. Still unsure about what it was, I took it out for closer examination. With careful study, suspected that it wasn’t a wet spot at all, perhaps a "loose" spot. There appeared to be depth there, as if that portion of the cake was not solid. I gave the jar a few jarring shakes, and hit it a few times on my palm. The cake loosened around that spot revealing more depth, as if it was an air bubble that had got trapped inside the cake. It was the weirdest thing. Positive the myc would grow no further in this situation I decided to birth it early. I removed the lid, dumped out the dry verm, then put the lid back on, to loosen the cake by hitting it on a sturdy box. When loose, I pulled it out of the jar over the trash can. Perhaps not the best idea in hind sight, but unfortunately for me I was in a hurry, and needed to move. I cleaned it off in filtered tap water, submerged it and put it in the fridge. I am interested to see if one strain prevailed, or if I will get two strains growing off the same cake. Maybe they will merge as one? I don’t really know, I think I'll read about that.

I did not have time to set up my fruiting chamber to have the climate stable by the next day. I will do that tonight, and the cake will have to deal with some fluctuations. I will probably take the heat rock from the incubator and put it in the fruiting chamber as popular vote says it's too warm in my incubator anyway. I’m not that worried, the incubator has been very good at keeping a steady climate, and the jars should be ready for birthing in a few days. If the temp fluctuates deviates too much I will go and buy another heat rock for the sake of being consistent in experimentation. I don’t want to change too much mid cycle.

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Batch 2 Update:

11 May (Day6) The jars have disappointed me slightly. I expected these smaller jars to colonize faster. I took more care in preparing them, and even used a generous amount of spore solution, not too much, but generous. They are certainly progressing, they just aren't living up to my expectations. The pints went much faster. Anyway, I became frustrated with having to remove these small jars to get a look at them due to the tin foil covering the sides. I decided to drill the jar lids and put them on. I did this, and cleaned each lid with alcohol, and carefully and cleanly applied the lids to each jar. I predict them to be 100% colonized in just under a week. And at this time, no signs of contams or problems at all. 
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Offlinedarkhawk37
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Re: Long time lurker, first grow log and introduction. [Re: Biostatik]
    #14439110 - 05/12/11 08:27 AM (12 years, 9 months ago)

good log and hows that peutro rican as far as colonizing goes ?

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InvisibleFrankHorrigan
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Registered: 01/04/11
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Re: Long time lurker, first grow log and introduction. [Re: Biostatik]
    #14439953 - 05/12/11 12:29 PM (12 years, 9 months ago)

Quote:

Biostatik said:
I will probably take the heat rock from the incubator and put it in the fruiting chamber as popular vote says it's too warm in my incubator anyway.




:badidea:
Heat differential between the inner and outer walls of your FC will lower your dewpoint, limiting your RH.
In other words, if your room is 65 and your FC is 75, your humidity in the FC is going to form condensation on the walls instead of staying in the air where it's needed.
For cakes, this is important because they require higher humidity than casings.
What is your room temp?
Unless it's below 60 I wouldn't worry, you're just going to have slightly slower, more potent, heavier yielding babies.


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Offlinek00laid
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Re: Long time lurker, first grow log and introduction. [Re: FrankHorrigan]
    #14439962 - 05/12/11 12:31 PM (12 years, 9 months ago)

heat the room not the fruiting chamber. or incubator.

but really unless your room temp is below 65 degrees.

you dont need to heat anything.


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Offlinedarkhawk37
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Re: Long time lurker, first grow log and introduction. [Re: k00laid]
    #14445094 - 05/13/11 11:38 AM (12 years, 9 months ago)

word dont need to throw a bacteria bomb on them i fruit at 65

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