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InvisibleLayinUp
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First run at LC
    #14445069 - 05/13/11 11:32 AM (12 years, 9 months ago)

So I had half a spore syringe left in my closet, it's about three weeks old so i'm kind of anxious to use it before it's not "fresh". I'm kind of in between projects at the moment, mostly due to waiting on items to arrive via mail.
  So what better way to use up these spores and learn something new than try a run at liquid culture. This way when my gear does arrive, i will already have viable mycelium ready to be nocd up.
After reading around a few teks, I decided to go with honey.
Mostly because that is what i have around the house. I weighed out 400grams/ml of water on my digi's, and added honey until it read 416.0 exactly. There seems to be some gunk floating around in the water, should i filter the solution through a coffee filter(s) before i load up my jars??
  I used 3 old 1/2pint jars from a pftek grow. I siliconed up the 4 innoc holes and drilled a 1/4 inch hole in the center, inserting a common pen tube. I siliconed around the tube now turned air port, and stuffed some polyfil in the tips pretty tightly.

  I figure i'll inoc through one of the 4 holes i siliconed up, since it seems to seal behind itself.
  So what do you all think? It will work? I didn't have the best materials but i'm hoping it will work.


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InvisibleTranscendingLife
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Re: First run at LC [Re: LayinUp]
    #14445082 - 05/13/11 11:35 AM (12 years, 9 months ago)

It sounds like it should work, but I've never used honey, nor never will. That's a waste of a delicious healthy sugar. I use (Extra) Light Malt Extract for all my LCs & they do great.

I wouldn't have your pen tube that's for GE stuffed down into the liquid either, as you don't want to get that wet, because it will cause contams.

Are you PCing the jars or doing the boil sterilization?


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InvisibleLayinUp
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Re: First run at LC [Re: TranscendingLife]
    #14445089 - 05/13/11 11:36 AM (12 years, 9 months ago)

PC @ 15psi for 15min :]
i just picked up a 23qt PC from the store yesterday, eager to try it out.


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InvisibleTranscendingLife
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Re: First run at LC [Re: LayinUp]
    #14445103 - 05/13/11 11:40 AM (12 years, 9 months ago)

Quote:

LayinUp said:
PC @ 15psi for 15min 30 min :]
i just picked up a 23qt PC from the store yesterday, eager to try it out.




Fixed it for ya...you want 30 minutes, 15 isn't enough...


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AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

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InvisibleLayinUp
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Re: First run at LC [Re: LayinUp]
    #14445114 - 05/13/11 11:43 AM (12 years, 9 months ago)

lol, good lookin out, i love the community here

 

better?


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InvisibleTranscendingLife
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Re: First run at LC [Re: LayinUp]
    #14445127 - 05/13/11 11:45 AM (12 years, 9 months ago)

yea lookin' a lot better, I'd recommend you stuff that poly as tightly as possible...& if you want to keep messing w/ LCs invest in some Whatmans & (E)LME, which can be found @ your local brewery.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

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Offlinek00laid
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Re: First run at LC [Re: LayinUp]
    #14445137 - 05/13/11 11:47 AM (12 years, 9 months ago)

polyfil only works as a gas exchange filter when its VERY TIGHTLY PACKED.

i would just make some proper grain lids, they can be used for LC as well.

2 holes

1 packed with fil 1 packed with silicone .


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InvisibleTranscendingLife
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Re: First run at LC [Re: k00laid]
    #14445146 - 05/13/11 11:49 AM (12 years, 9 months ago)

Quote:

k00laid said:
polyfil only works as a gas exchange filter when its VERY TIGHTLY PACKED.

i would just make some proper grain lids, they can be used for LC as well.

2 holes

1 packed with fil 1 packed with silicone .




Or just got buy a pack of 12 100mm SFDs from everythingmushrooms.com & cut those & use those...:2cents: I hate poly or tyvek for lids, I only use SFDs or Whatmans


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

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InvisibleLayinUp
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Re: First run at LC [Re: k00laid]
    #14445150 - 05/13/11 11:50 AM (12 years, 9 months ago)

way ahead of you there,

just waiting on some fancy self healing injection points to arrive in the mail.


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Offlinek00laid
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Re: First run at LC [Re: TranscendingLife]
    #14445153 - 05/13/11 11:50 AM (12 years, 9 months ago)

i sew, soo polyfil is abundant :P


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InvisibleTranscendingLife
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Re: First run at LC [Re: k00laid]
    #14445182 - 05/13/11 11:59 AM (12 years, 9 months ago)

Quote:

LayinUp said:
way ahead of you there,

just waiting on some fancy self healing injection points to arrive in the mail.




I just use RTV silicone for my inoc points...
I like it way better than those fancy blue things, which die out too quickly & you end up filling in the bottom w/ silicone anyways.


Quote:

k00laid said:
i sew, soo polyfil is abundant :P




I don't think I even need to comment on that? :ifyoucanawe:


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

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Offlinek00laid
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Re: First run at LC [Re: TranscendingLife]
    #14445215 - 05/13/11 12:13 PM (12 years, 9 months ago)

:rofl:

i get all sorts of shit for it. in fact i get all sorts of shit for how feminine i am in general >.>


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InvisibleLayinUp
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Re: First run at LC [Re: TranscendingLife]
    #14445220 - 05/13/11 12:13 PM (12 years, 9 months ago)

how big do you drill your holes for the rtv inoc points?


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InvisibleTranscendingLife
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Re: First run at LC [Re: LayinUp]
    #14445230 - 05/13/11 12:15 PM (12 years, 9 months ago)

1/4" for the blue inoculation ports I used 1/2" I think, can't quite remember, as I only used them once.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

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Offlinerustycobwebs
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Re: First run at LC [Re: TranscendingLife]
    #14445255 - 05/13/11 12:22 PM (12 years, 9 months ago)

Finishing nail was quicker for me than figuring out which drill bit to use.

Drill a hole with your smallest drill bit, take your biggest syringe and try to push it through. It should have a little room and not be scraping the edges. if it does, you're good to go.


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InvisibleLayinUp
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Re: First run at LC [Re: rustycobwebs]
    #14445330 - 05/13/11 12:38 PM (12 years, 9 months ago)

i feel like they may not be 'self sealing' if there isnt a certain amount of silicone to penetration ratio if there is such a concept im no expert...


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InvisibleTranscendingLife
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Re: First run at LC [Re: LayinUp]
    #14445484 - 05/13/11 01:18 PM (12 years, 9 months ago)

Quote:

LayinUp said:
i feel like they may not be 'self sealing' if there isnt a certain amount of silicone to penetration ratio if there is such a concept im no expert...





As long as you don't dislodge the silicone from the lid during inoculation/piercing w/ a needle, you'll be fine.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

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OfflineiSHACKA
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Re: First run at LC [Re: TranscendingLife]
    #14445737 - 05/13/11 02:08 PM (12 years, 9 months ago)

try turbinado sugar, or RAW sugar. Super easy, super clear. Honey is all cloudy.

Regardless honey can work just my two cents


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Offlinek00laid
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Re: First run at LC [Re: iSHACKA]
    #14445749 - 05/13/11 02:11 PM (12 years, 9 months ago)

sugar in general is sub-par for mycelial cultures.

try dextrose or light malt extract


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OfflineiSHACKA
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Re: First run at LC [Re: k00laid]
    #14445782 - 05/13/11 02:17 PM (12 years, 9 months ago)

would you say its equal to honey tho, sugar?

All my honey turned out like ass, as soon as i switched to turbinado everything took off. Im an amature tho, so this is just my experiance.


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