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InvisiblePinsWellWithOthers
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Agar samples
    #14441340 - 05/12/11 06:08 PM (12 years, 9 months ago)

Wanted to post a sample im working with currently. What points would you sample from and how long from current stage. (~35mm in size on a 100mm plate)
Front

Back and flips horizontally, Slight turned to the right.

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InvisibleFooMan
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Re: Agar samples [Re: PinsWellWithOthers]
    #14441495 - 05/12/11 06:39 PM (12 years, 9 months ago)

On the top pick I would go with the 11 o'clock and 4-5 o'clock positions and I would do it now.


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InvisiblePinsWellWithOthers
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Re: Agar samples [Re: PinsWellWithOthers]
    #14441909 - 05/12/11 07:56 PM (12 years, 9 months ago)

Is each rope of rizomorphic growth a strain and should I transfer all of the rizomorphic ropes to new dishes or just the outer edge areas of desired growth? (hard to see just how ropy it looks from the slight condensation on the dish cover.)

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Invisibleconformist
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Re: Agar samples [Re: PinsWellWithOthers]
    #14442199 - 05/12/11 08:52 PM (12 years, 9 months ago)

It seems to me that the sectors are more easily seen if you shine the light through the dish (from behind) while looking at the bottom.

Testing really depends on how organized you want to get. You can make several transfers and isolate further sectors till no end.

I would suggest making some successful transfers, then cut up the original plate to some grain for testing. If you get fruit you can clone/print for future.

As long as you have plenty of spores, you'll have more mycelium than you know what to do with.

Too many decisions....

Each "rope" could possibly be a different strain, but they tend to separate themselves as far as rate of growth. There again, you could isolate each and every one and test, but you may go crazy.

Edited by conformist (05/12/11 08:58 PM)

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InvisiblePinsWellWithOthers
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Re: Agar samples [Re: conformist]
    #14442567 - 05/12/11 10:04 PM (12 years, 9 months ago)

Well this is the 5th transfer from a mutispore dish. I have something like 50 prints to work with and they are all very dark and big so i guess its a matter of how far i want to isolate. is there a difference between when i select for transfer early rather then late? I know RR said mycelium that is compatible with other mycelium will like (not word for word) mix with each other to make a slightly different mycelium but at what level does this happen? is this why people recommend early transfers?

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Invisiblewygram
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Re: Agar samples [Re: PinsWellWithOthers]
    #14442924 - 05/12/11 11:13 PM (12 years, 9 months ago)

This pretty much sums it up. The first part can also apply to your question about early transfers. You want to catch and separate different strains early, before they begin to mix.

Quote:

RogerRabbit said:
As for cloning vs strain isolation, they're not related.  By the time a substrate fruits, hundreds or perhaps thousands of strains have exchanged DNA, either weakening or strengthening the mass.  What you get is a 'heinz 57' that may or may not be that great because the weaker genes and the stronger genes(mycelium) have all combined.  An example would be mixed breed dogs.  We've all seen good examples and others that are dumber then hell.

Strain isolation on agar begins when the spores first start to germinate.  I make the first transfers as soon as I can see mycelium growing from the point of inoculation, long before sectoring can be detected.  By doing this, and by continuing to separate each individual growth, you can isolate mycelium prior to the process of anastomosis combining dikaryons into a single mass.

You don't isolate looking for one super rhizomorphic strain.  You isolate down to single sectors and then fruit out each one to determine the best performer.  When you transfer mycelium to a grain master, the original petri dish the mycelium was taken from is placed into a clean refrigerator.  By doing this, when you find the best performing strain, you then go back to your well marked petri dishes, thus your original P1 culture.  This petri dish can be used to inoculate a few test tube slants that can be incubated for a week, then placed in cold storage.  Whenever you need mycelium, a tiny piece the size of a grain of rice can be taken from the test tube and put on agar to grow out, while the test tube is placed back into the refrigerator.  These stored test tube cultures preserve the low P value of your isolated strain for years.




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InvisiblePinsWellWithOthers
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Re: Agar samples [Re: wygram]
    #14444168 - 05/13/11 08:01 AM (12 years, 9 months ago)

Thank you for all the halp everyone. Ill get started on that today then

Edited by PinsWellWithOthers (05/13/11 08:15 AM)

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