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Invisiblesavage.renegade
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Registered: 07/02/10
Posts: 986
Loc: Bay area
question about cloning
    #14441537 - 05/12/11 06:46 PM (12 years, 8 months ago)

So im about to begin working with isolates on agar.

My question is if I take a sample from the inner stipe of a single mushroom and put on agar, is that already an isolate? Or is it possible for a single fruit from a MS tray to be more than one strain at a time?

Much pride and respect


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Invisibleconformist
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Registered: 07/29/10
Posts: 210
Loc: SE Flag
Re: question about cloning [Re: savage.renegade]
    #14442072 - 05/12/11 08:23 PM (12 years, 8 months ago)

From what I understand, cloning from a mushroom could sector out into multiple strains (or even substrains) from which you could isolate and test. Although you could get lucky and get a single strain.

I'm not sure how rare it is but I recently found some wild Oyster mushrooms, transferred five pieces of tissue to agar of which only one produced a perfect culture (the rest turned to mush). Talk about lucky.

You're getting into the fun stuff, show us your work.

Good Luck and enjoy.


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Invisiblesavage.renegade
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Registered: 07/02/10
Posts: 986
Loc: Bay area
Re: question about cloning [Re: conformist]
    #14442121 - 05/12/11 08:33 PM (12 years, 8 months ago)

Thanx for clearing that up for me. I cant wait to fruit a tray from an isolate. Been putting off agar work to long.


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