Home | Community | Message Board

MushroomCube.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineSteelcutoats
Stranger
Male


Registered: 04/28/11
Posts: 60
Last seen: 12 years, 7 months
SteelCutOat's Grow Log---Retard TEK
    #14438360 - 05/12/11 02:01 AM (12 years, 9 months ago)

This is refering to the research of cultivation of legal fungus for academic and intellectual purposes.

First I made a liquid culture from a spore syringe by boiling jars of 4% honey/water, inoculating and taping the hole. One jar had gas exchange while two did not. The two without GE grew significantly faster than the one with GE.

Took Cracked Corn and Verm and mixed in a pot. Boiled for 15 mins. Then mixture, still hot, is moved to a cermaic bowl with a matching bowl on top and oven cooked at 350 degrees for 1 hour.

Boiled my 1 cup PP containers for 15 mins.

Lids: Poked two holes in each lid with the needle matching my empty syringe. Then three stacked coffee filters were rubber banded to the lid's edges. Put up to a light I marked the holes in the lid on the coffee filter with a sharpie marker.

Placed an even amount of CC/Verm in each containter and screwed the lid on tight caring not to remove the coffee filters. Boiled for 1.5 hours and plastic bagged and left to cool in early afternoon.

11:00 pm Monday May 9: Inoculated each container with liquid culture. Taped the hole in the coffee filters.

Incubated at 86 degrees.

12:00 am Thurs May 12: With a microscope eyepiece lens I inspected my containers. Many of them have stringy, ropey, bright white rhizomorphic explosion points, though barely visible to the naked eye (therefore no pics yet).

Will update with pics soon.


--------------------
I don't fail. I succeed at finding what doesn't work.

Extras: Filter Print Post Top
InvisibleLuger0815
noob
Male

Registered: 12/21/10
Posts: 1,677
Loc: @ Home
Re: SteelCutOat's Grow Log---Retard TEK [Re: Steelcutoats]
    #14438369 - 05/12/11 02:04 AM (12 years, 9 months ago)

86 degrees is too high for incubation.
75F is more ideal, if you dont plan to breed bacteria.

Extras: Filter Print Post Top
Invisibletechnics1200
Stranger

Registered: 02/23/11
Posts: 5
Re: SteelCutOat's Grow Log---Retard TEK [Re: Luger0815]
    #14438401 - 05/12/11 02:19 AM (12 years, 9 months ago)

without Pressure cooking any grain I would think that you are opening the door for very quick contamination.

Extras: Filter Print Post Top
InvisibleLuger0815
noob
Male

Registered: 12/21/10
Posts: 1,677
Loc: @ Home
Re: SteelCutOat's Grow Log---Retard TEK [Re: technics1200]
    #14438421 - 05/12/11 02:27 AM (12 years, 9 months ago)

:whathesaid:

Extras: Filter Print Post Top
OfflineSteelcutoats
Stranger
Male


Registered: 04/28/11
Posts: 60
Last seen: 12 years, 7 months
Re: SteelCutOat's Grow Log---Retard TEK [Re: Luger0815]
    #14438458 - 05/12/11 02:41 AM (12 years, 9 months ago)

I've always wondered, if the lid on the jar is secured tightly, doesn't the jar itself become pressurized through heating?


--------------------
I don't fail. I succeed at finding what doesn't work.

Extras: Filter Print Post Top
InvisibleLuger0815
noob
Male

Registered: 12/21/10
Posts: 1,677
Loc: @ Home
Re: SteelCutOat's Grow Log---Retard TEK [Re: Steelcutoats]
    #14438537 - 05/12/11 03:20 AM (12 years, 9 months ago)

You would risk that the jars blow up if you close them airtight and heat them above the boiling point.
As long as there is any humidity into the grain, the temp will not rise above 100C.
That is not enough to kill endospores.
In a PC at 15 PSI the temp is about 130F which is enough to kill the common endospores.
You need to hold the 130C for at least 30 minutes to get all endospores killed.

Extras: Filter Print Post Top
OfflineSteelcutoats
Stranger
Male


Registered: 04/28/11
Posts: 60
Last seen: 12 years, 7 months
Re: SteelCutOat's Grow Log---Retard TEK [Re: Luger0815]
    #14438565 - 05/12/11 03:33 AM (12 years, 9 months ago)

Are you intentionally using Centigrade and Fahrenheit interchangeably?

130F = 54.44C

130C = 266F -this is what I think you meant.


--------------------
I don't fail. I succeed at finding what doesn't work.

Extras: Filter Print Post Top
OfflineSteelcutoats
Stranger
Male


Registered: 04/28/11
Posts: 60
Last seen: 12 years, 7 months
Re: SteelCutOat's Grow Log---Retard TEK [Re: Steelcutoats]
    #14438567 - 05/12/11 03:35 AM (12 years, 9 months ago)

From WIKI---While resistant to extreme heat and radiation, endospores can be destroyed by burning or by autoclaving. Endospores are able to survive boiling at 100°C for hours, although the longer the number of hours the fewer that will survive.


--------------------
I don't fail. I succeed at finding what doesn't work.

Extras: Filter Print Post Top
InvisibleLuger0815
noob
Male

Registered: 12/21/10
Posts: 1,677
Loc: @ Home
Re: SteelCutOat's Grow Log---Retard TEK [Re: Steelcutoats]
    #14438596 - 05/12/11 04:06 AM (12 years, 9 months ago)

I suggest to buy a PC.
It is necessary for sterilizing grains.
Or try this..Fractional sterilization or tyndallization

Extras: Filter Print Post Top
OfflinePerun
Mahapralaya...
Male User Gallery


Registered: 11/11/10
Posts: 585
Last seen: 2 years, 3 months
Re: SteelCutOat's Grow Log---Retard TEK [Re: Luger0815]
    #14438600 - 05/12/11 04:11 AM (12 years, 9 months ago)

Buy a PC if you want to go anywhere with grain!

If you dont want to buy PC...go with BRF cakes!


--------------------
First:                                                        Then:

Extras: Filter Print Post Top
OfflineSteelcutoats
Stranger
Male


Registered: 04/28/11
Posts: 60
Last seen: 12 years, 7 months
Re: SteelCutOat's Grow Log---Retard TEK [Re: Perun]
    #14438749 - 05/12/11 05:50 AM (12 years, 9 months ago)

LOL Open your mind a little bit to different possibilities. Just because pressure cooking is the easiest, most sterile, and quickest way (therefore best way)....it does not necessarily entail it to be the ONLY way.


--------------------
I don't fail. I succeed at finding what doesn't work.

Extras: Filter Print Post Top
OfflineMaharishi_2_U
Opt Out Super Fag
Male User Gallery

Registered: 10/21/09
Posts: 6,316
Loc: The Streets Flag
Last seen: 8 years, 11 months
Re: SteelCutOat's Grow Log---Retard TEK [Re: Steelcutoats]
    #14438769 - 05/12/11 06:00 AM (12 years, 9 months ago)

Quote:

Steelcutoats said:
Just because pressure cooking is the easiest, most sterile, and quickest way (therefore best way)....it does not necessarily entail it to be the ONLY way.



I'd be willing to bet buds to buttons that the "close minded" posters are well aware of this statement :smile:
the title of this website says something to the effect of
"spreading ACCURATE information....."
I think that is what you are seeing.
Good luck with your project :smile:
BC

Extras: Filter Print Post Top
OfflineSteelcutoats
Stranger
Male


Registered: 04/28/11
Posts: 60
Last seen: 12 years, 7 months
Re: SteelCutOat's Grow Log---Retard TEK [Re: Maharishi_2_U]
    #14438774 - 05/12/11 06:03 AM (12 years, 9 months ago)

lol I'm trying soo hard. I just haven't gotten my hands on a PC yet. Besides...it's working so far. I will post pics as soon as you can see stuff with the naked eyeballs


--------------------
I don't fail. I succeed at finding what doesn't work.

Extras: Filter Print Post Top
Offlinesoli
rogue psychologist
 User Gallery

Registered: 05/18/06
Posts: 280
Last seen: 7 years, 9 months
Re: SteelCutOat's Grow Log---Retard TEK [Re: Luger0815]
    #14438775 - 05/12/11 06:04 AM (12 years, 9 months ago)

Quote:

Luger0815 said:
86 degrees is too high for incubation.
75F is more ideal, if you dont plan to breed bacteria.




eh.. 75 is fruiting temp. incubation shold be 80-82 for quick colonization. 85 is a bit high but not TOO High.

my jars take 3-4 weeks to colonize below 79F ; 1.5-2 weeks at 82F


--------------------
irc,partydome.us #irc

Extras: Filter Print Post Top
InvisibleLuger0815
noob
Male

Registered: 12/21/10
Posts: 1,677
Loc: @ Home
Re: SteelCutOat's Grow Log---Retard TEK [Re: soli]
    #14438783 - 05/12/11 06:08 AM (12 years, 9 months ago)

.

Edited by Luger0815 (07/04/11 11:08 AM)

Extras: Filter Print Post Top
InvisibleLuger0815
noob
Male

Registered: 12/21/10
Posts: 1,677
Loc: @ Home
Re: SteelCutOat's Grow Log---Retard TEK [Re: Luger0815]
    #14438789 - 05/12/11 06:10 AM (12 years, 9 months ago)

.

Edited by Luger0815 (07/04/11 11:07 AM)

Extras: Filter Print Post Top
Offlineash057
dusted


Folding@home Statistics
Registered: 10/02/10
Posts: 77
Last seen: 5 years, 5 months
Re: SteelCutOat's Grow Log---Retard TEK [Re: Luger0815]
    #14438791 - 05/12/11 06:11 AM (12 years, 9 months ago)

:tard:TEK

Extras: Filter Print Post Top
OfflineSteelcutoats
Stranger
Male


Registered: 04/28/11
Posts: 60
Last seen: 12 years, 7 months
Re: SteelCutOat's Grow Log---Retard TEK [Re: ash057]
    #14438796 - 05/12/11 06:13 AM (12 years, 9 months ago)

Actually the Tyndall method looks pretty good to me. Will use on next batch when...I mean...if this one fails.


--------------------
I don't fail. I succeed at finding what doesn't work.

Extras: Filter Print Post Top
InvisibleLuger0815
noob
Male

Registered: 12/21/10
Posts: 1,677
Loc: @ Home
Re: SteelCutOat's Grow Log---Retard TEK [Re: Steelcutoats]
    #14438805 - 05/12/11 06:19 AM (12 years, 9 months ago)

.

Edited by Luger0815 (07/04/11 11:07 AM)

Extras: Filter Print Post Top
OfflineSteelcutoats
Stranger
Male


Registered: 04/28/11
Posts: 60
Last seen: 12 years, 7 months
Re: SteelCutOat's Grow Log---Retard TEK [Re: Luger0815]
    #14438823 - 05/12/11 06:24 AM (12 years, 9 months ago)

Are the grains totally submerged in between boiling? Or left to dry?


--------------------
I don't fail. I succeed at finding what doesn't work.

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* An introduction and Mazapatec mono grow log
( 1 2 3 4 all )
grow4fun 6,185 73 04/21/18 08:54 AM
by grow4fun
* retard tek (pic) puscle 2,332 14 05/26/01 03:44 AM
by Sterile
* DB kit Growing log... Proteus 3,710 15 11/12/01 09:50 PM
by Proteus
* Hydro-Pod newbie grow log
( 1 2 3 4 5 all )
thedm9 20,810 80 09/26/01 02:14 PM
by CACA
* grow log! phil2k1 2,628 5 03/23/02 08:41 PM
by phil2k1
* Invitro Bulk Tek Version 1.2 ShroomZilla007 19,571 12 05/15/23 04:54 AM
by Stromrider
* I want your logs! fr0zbnitz 2,459 8 07/18/01 07:31 PM
by BrownPastures
* Hippies invitro-tek BladeLSD 9,624 4 08/30/01 10:09 AM
by puscle

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
757 topic views. 21 members, 138 guests and 27 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.009 seconds on 13 queries.