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Offlinezeemped
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Registered: 04/18/11
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Last seen: 11 years, 1 month
stunted incubation?? 5 weeks and still waiting
    #14433752 - 05/11/11 07:59 AM (12 years, 8 months ago)

Hi all, I have 8 pf cakes incubating right now at 83-86 degrees f. They have been incubation now for soon to be 6 full weeks and none are fully caked yet. There are two holes in each lid for inoculation. Four jars used 1.5 cc b+ spores and the other four I used 1cc. How can I get my batches to incubate faster? Is there anyway to boost the speed of this current pf batch also? Thanks for any advice!


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OfflineSmokey-hitz
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Re: stunted incubation?? 5 weeks and still waiting [Re: zeemped]
    #14433764 - 05/11/11 08:05 AM (12 years, 8 months ago)

Put four holes next time, shake syringe real good. And if they are slow you can flip jar over on an extra lid so that the co2 leaves the jar more


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OfflineBstsneverr
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Re: stunted incubation?? 5 weeks and still waiting [Re: Smokey-hitz]
    #14433798 - 05/11/11 08:20 AM (12 years, 8 months ago)

Isn't incubation out-dated? Not saying you should not incubate them...  Just IME you can tuck them wherever, if u can walk around and you dont feel cold, the jars will do just fine. :thumbup: Do you have pictures?


--------------------
*** Psalm 9:9-10 ***

The lord is a refuge for the oppressed, a stronghold in times of trouble.

Those who know your name will trust in you, for you, lord, have not forsaken those who seek you.


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OfflineSpin_Dr
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Re: stunted incubation?? 5 weeks and still waiting [Re: zeemped]
    #14433855 - 05/11/11 08:36 AM (12 years, 8 months ago)

I would also suggest that you try to get them in a place that sets at a stable temp in the house. for example, you could put them in the cupboard above the refrigerator. I have a spot that I can maintain the temp between 75 and 80 degrees and have great results.


--------------------
The Spin Dr.

I feel more like I do now than I did when I got here.


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Offlinezeemped
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Registered: 04/18/11
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Re: stunted incubation?? 5 weeks and still waiting [Re: Spin_Dr]
    #14433905 - 05/11/11 08:53 AM (12 years, 8 months ago)

I'm pretty new to this. What is IME?  Also I am shakin and flipping the jars every few days to try to speed growth. I can post pick later this afternoon


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Offlinesteelmonkey
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Re: stunted incubation?? 5 weeks and still waiting [Re: zeemped]
    #14434869 - 05/11/11 01:07 PM (12 years, 8 months ago)

IME=in my experience and please stop shaking your jars

besides the inoculation points do you have some kind of filter for Gas Exchange?


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Offlinezeemped
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Re: stunted incubation?? 5 weeks and still waiting [Re: steelmonkey]
    #14434990 - 05/11/11 01:36 PM (12 years, 8 months ago)

To shake or not to shake? Why are there so many contradictory threads. Many saying shaking helps. Many saying shaking is bad. Which shold I believe?

And my only air exchange filter is the two holes covered with tin foil. Should I punch more holes?


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OfflineAcidOnTap
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Registered: 05/10/11
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Re: stunted incubation?? 5 weeks and still waiting [Re: zeemped]
    #14440663 - 05/12/11 03:27 PM (12 years, 8 months ago)

I have them in a big plastic tub with pretty decent verm layer, tin foil with noc holes, and lid on container theyre in. This sounds about right?? Any suggestions?  Wish me luck

Also getting ready to LC a few jars see how it works out. Honey jars.


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Offlinenushie
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Re: stunted incubation?? 5 weeks and still waiting [Re: AcidOnTap]
    #14440762 - 05/12/11 03:56 PM (12 years, 8 months ago)

hmmm so many things wrong is the reason, I cant believe they arent green yet....  Incubators are out of date and cause contams.  83-86 degrees is way to hot!  The inside of the jars are up to 3 degrees warmer so u actual temp range is 86-89.  BRF is not to be shaken.  WBS and other grains are shaken,  Id start over man.....


Edited by nushie (05/12/11 03:57 PM)


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Offlinesteelmonkey
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Re: stunted incubation?? 5 weeks and still waiting [Re: AcidOnTap]
    #14440767 - 05/12/11 03:57 PM (12 years, 8 months ago)

To the last two poster...you need more than just tinfoil to cover your inoculation holes if they are just punched hole you atleast need them stuffed with poly
The tinfoil is only in place to prevent moisture from entering during PCing after you inoculate you take the foil off

zeemped-- You shake your jars when inoculating with LC,With MS you don't shake


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OfflineAcidOnTap
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Registered: 05/10/11
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Re: stunted incubation?? 5 weeks and still waiting [Re: steelmonkey]
    #14444112 - 05/13/11 07:40 AM (12 years, 8 months ago)

I been watching my jars. Its only been like 40 hours. The only thing Im noticing it in one of the jars it appears that there is a visible black piece of spore i injected from the syringe.

It must be because it has little white arms shooting off it. Can this really be some spore forming mycelium or am I looking at black mold.

Nothing in the rest of jars but Im pretty damn certain thats what it is. There was a lot of spores in my syringes so Im really not surprised actually.

Anybody have any comments or concerns? I should probably keep any eye on it. As long as it gets whiter and not blacker I guess it should be ok.


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OfflineElevated
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Re: stunted incubation?? 5 weeks and still waiting [Re: AcidOnTap]
    #14444453 - 05/13/11 09:25 AM (12 years, 8 months ago)

Quote:

AcidOnTap said:
I been watching my jars. Its only been like 40 hours. The only thing Im noticing it in one of the jars it appears that there is a visible black piece of spore i injected from the syringe.

It must be because it has little white arms shooting off it. Can this really be some spore forming mycelium or am I looking at black mold.

Nothing in the rest of jars but Im pretty damn certain thats what it is. There was a lot of spores in my syringes so Im really not surprised actually.

Anybody have any comments or concerns? I should probably keep any eye on it. As long as it gets whiter and not blacker I guess it should be ok.




sounds like mycelium, check again in a day or two


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Offlinek00laid
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Re: stunted incubation?? 5 weeks and still waiting [Re: zeemped]
    #14444556 - 05/13/11 09:54 AM (12 years, 8 months ago)

Quote:

zeemped said:
To shake or not to shake? Why are there so many contradictory threads.




the only threads that contradict are threads that are years old.

look at the date of the information you are reading. that tends to give you some understanding of what it is you are actually reading.

if a thread from 5 years ago tells you to shake your jars every day.

and a thread from 2 days ago tells you not to.

which do you think you should believe?


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AMU - AMU Q & A - MyVideo Teks!


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OfflineAcidOnTap
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Registered: 05/10/11
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Last seen: 11 years, 21 days
Re: stunted incubation?? 5 weeks and still waiting [Re: Elevated]
    #14444576 - 05/13/11 09:59 AM (12 years, 8 months ago)

thank you, yea i took a closer look and it seems that it is growing in towards the center of the jar as well. Im pretty convinced though Id like to see progress. Exciting.

AOT


Edited by AcidOnTap (05/13/11 10:03 AM)


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OfflineAcidOnTap
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Re: stunted incubation?? 5 weeks and still waiting [Re: AcidOnTap]
    #14447319 - 05/13/11 07:20 PM (12 years, 8 months ago)

Well the black spot is visibly getting bigger. There is actually like three little spots and they are as a matter of fact straight down from the noc holes. even after like 8 hours visible growth. Nothing from the the other jars though. Its only been 2 days though I prolly got lucky with the one.

Also waiting on the LC to get going and whether or not that will be a success.

Loving the science aspect of it I hope to get a little more advanced on the next run without burning my pockets.

I even have this baby thermometer that goes across the forehead I been using to measure the temp of the jars. Not doing too bad if I do say so myself.


Edited by AcidOnTap (05/13/11 07:23 PM)


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OfflineBstsneverr
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Re: stunted incubation?? 5 weeks and still waiting [Re: AcidOnTap]
    #14448314 - 05/13/11 09:59 PM (12 years, 8 months ago)

lol visible black spore that gets bigger? Wrong my friend... What might be a "Visible" spore is actually probably 3 or 4 hundred. And spores do not get bigger. Thats mold my friend.


--------------------
*** Psalm 9:9-10 ***

The lord is a refuge for the oppressed, a stronghold in times of trouble.

Those who know your name will trust in you, for you, lord, have not forsaken those who seek you.


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OfflineBstsneverr
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Registered: 03/22/08
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Re: stunted incubation?? 5 weeks and still waiting [Re: Bstsneverr]
    #14448351 - 05/13/11 10:06 PM (12 years, 8 months ago)

I might as well add that black mold. Is not good. Get it out of your house.


--------------------
*** Psalm 9:9-10 ***

The lord is a refuge for the oppressed, a stronghold in times of trouble.

Those who know your name will trust in you, for you, lord, have not forsaken those who seek you.


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OfflineAcidOnTap
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Registered: 05/10/11
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Last seen: 11 years, 21 days
Re: stunted incubation?? 5 weeks and still waiting [Re: Bstsneverr]
    #14453328 - 05/14/11 08:19 PM (12 years, 8 months ago)

the black is not getting bigger its the white surrounding it getting bigger. Its all gravy. Its a cluster from the syringe.


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OfflineAcidOnTap
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Re: stunted incubation?? 5 weeks and still waiting [Re: AcidOnTap]
    #14453344 - 05/14/11 08:22 PM (12 years, 8 months ago)

i would say definitely a negative on the shaking brf jars if you think about it the verm layer is filtering so if theres anything on the top shake just mixes it right in to take over the cake.

The LC though I have my questions about shaking that and I would assume it about a good 3-4 days before seeing that take over just like regular germination with any spore?


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OfflineAcidOnTap
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Registered: 05/10/11
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Re: stunted incubation?? 5 weeks and still waiting [Re: AcidOnTap]
    #14464453 - 05/16/11 08:19 PM (12 years, 8 months ago)

The jars. looking good five days. Peurto ricans no sign yet.



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OfflineWing
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Registered: 04/25/11
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Re: stunted incubation?? 5 weeks and still waiting [Re: AcidOnTap]
    #14465219 - 05/16/11 10:25 PM (12 years, 8 months ago)

take the foil off


--------------------
My Old Grow Logs



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OfflineElevated
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Re: stunted incubation?? 5 weeks and still waiting [Re: Wing]
    #14466525 - 05/17/11 06:41 AM (12 years, 8 months ago)

Quote:

winged_1 said:
take the foil off




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