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InvisibleJavadog
Continuing along
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Registered: 05/03/10
Posts: 7,385
Loc: USA Flag
Trusted Cultivator
Re: IS IT THE SYRINGES OR THE LIDS [Re: skiddy]
    #14417812 - 05/08/11 01:50 AM (12 years, 9 months ago)

Quote:

skiddy said:
OK first saying he bought the wrong spores labled for microscopy  is bullshit, there labeled for microscopy use so there able to sell them legally.... they should be viable under this label .




I thought that there was some joke going on.

Spores are spores.

Odd.

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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OfflineJonnyLongboard
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Registered: 04/19/10
Posts: 126
Loc: Yeti compound
Last seen: 11 years, 1 month
Re: IS IT THE SYRINGES OR THE LIDS [Re: Javadog]
    #14418798 - 05/08/11 10:06 AM (12 years, 9 months ago)

Yeah I know that is bullshit.  Any syringe you buy from anywhere is a "microscopy" syringe. Except for edibles.  If you want to see a pic of the jars just go to noobies lid tek and check them out.  The only difference is mine have red vacutainer injection ports instead of the blue ones from science surplus.  Im pretty sure my grain is organic, I get it from a brew shop in my area.  It's the same grain that ive used since I started doing this and  except for the first time I did it and had the grain to wet, growth has come at a pretty normal rate.  And even when the grain was way too wet it still grew eventually.


--------------------
"Lets get down to brass tacks, how much for the ape?"

Everything I say on this forum is completly theorectical. A complete fictionilization.

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InvisibleBoozie
I like beer.
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Registered: 02/18/10
Posts: 1,226
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Re: IS IT THE SYRINGES OR THE LIDS [Re: JonnyLongboard]
    #14424648 - 05/09/11 01:25 PM (12 years, 9 months ago)

That was going to be my next question--if it's possible the grain you're using is treated in some way. But if you've used the same stuff before with success, then there goes that theory.

Maybe try to germinate the spores on agar? That will eliminate a lot of variables and it's easy enough to transfer if you come up with something healthy-looking.

Have you opened the jars yet? Any unpleasant odors?

Out of curiosity, do you use a still-air/glove box or flowhood?


--------------------
"After silence, that which comes nearest to expressing the inexpressible is music." - Aldous Huxley


   

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Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: IS IT THE SYRINGES OR THE LIDS [Re: sandman420]
    #14424872 - 05/09/11 02:17 PM (12 years, 9 months ago)

Quote:

sandman420 said:
I've had several sporeworks spores that werent worth a shit. I got my mexicana print from them, it was absolutely worthless. 4 little prints on a square of foil that were so faint you couldnt even see them unless you turned it just right in the light. Im talking ridiculously light. It wouldnt have been a big deal if I could have gotten them to germinate on agar...But nope they would never germinate. Then there was my PEU syringe, hopelessly contaminated with what I guess is bacteria. Then there was the APE syringe, same thing. I used to have good luck with Sporeworks, but for the last year or so they have been worthless to me.




There is some truth to this even tho I am generally a big fan / advocate of the works.

Every APE or PEU syringe I've gotten from them has some degree of bacterial contamination. I believe it's their method of gathering the spores from the PE strains, probably gill irrigation, which is far from a sterile procedure.

The answer is to go straight to agar with em every time and not waste your time trying to take them directly to LC or even grain.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

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OfflineKrizzKaliko
Lurker
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Registered: 04/15/11
Posts: 192
Last seen: 6 years, 10 months
Re: IS IT THE SYRINGES OR THE LIDS [Re: PinsWellWithOthers]
    #14424977 - 05/09/11 02:40 PM (12 years, 9 months ago)

Quote:

PinsWellWithOthers said:
Clearly the problem is your using microscopy samples for cultivation use. Next time you should select a sample of spores for cultivation instead of microscopic study. There is also a slight chance that it could have been something entirely different. Spores>Agar will save you a lot of guess work if your are able to do it. do it.



:urstupid:

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OfflineJonnyLongboard
Habadasher

Registered: 04/19/10
Posts: 126
Loc: Yeti compound
Last seen: 11 years, 1 month
Re: IS IT THE SYRINGES OR THE LIDS [Re: Boozie]
    #14426405 - 05/09/11 07:27 PM (12 years, 9 months ago)

Quote:

Boozie said:
That was going to be my next question--if it's possible the grain you're using is treated in some way. But if you've used the same stuff before with success, then there goes that theory.

Maybe try to germinate the spores on agar? That will eliminate a lot of variables and it's easy enough to transfer if you come up with something healthy-looking.

Have you opened the jars yet? Any unpleasant odors?

Out of curiosity, do you use a still-air/glove box or flowhood?



I know that agar is a very good method but  it doesn't have to be done.  Most people dont ever use it and have no problems at all.  And like I said Ive used the same exact rye and same exact method and had no problems at all.  But I am going to try to call the rye producer and try to ask someone there if they perhaps began treating there rye with any fungicide's.  But I dont know if they would or not because it is being bought by a brew shop and you would think that you wouldn't want to brew anything using grain that has been treated with something like that but maybe it doesn't matter.  It has to be the rye or the spores because I know its not my lids or prep tek.  I know that im not RR or a master but my grain prep is pretty damn good nowadays and I know that there is a pretty damn good environment for mycelial growth in those jars and there isn't jack shit happening.  Its really driving me up a wall.  I need this shit to be going.


--------------------
"Lets get down to brass tacks, how much for the ape?"

Everything I say on this forum is completly theorectical. A complete fictionilization.

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InvisiblePinsWellWithOthers
Thread Derailer

Folding@home Statistics
Registered: 10/15/10
Posts: 1,834
Re: IS IT THE SYRINGES OR THE LIDS [Re: Javadog]
    #14433732 - 05/11/11 07:52 AM (12 years, 9 months ago)

Quote:

Javadog said:
Quote:

skiddy said:
OK first saying he bought the wrong spores labled for microscopy  is bullshit, there labeled for microscopy use so there able to sell them legally.... they should be viable under this label .




I thought that there was some joke going on.

Spores are spores.

Odd.

JD



Atleast someone got the joke. Wellplayed JD

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