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OfflineJonnyLongboard
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Registered: 04/19/10
Posts: 126
Loc: Yeti compound
Last seen: 11 years, 1 month
IS IT THE SYRINGES OR THE LIDS
    #14415373 - 05/07/11 01:55 PM (12 years, 9 months ago)

Well thats two sporeworks syringes that are bunk.  Its the spores or lids that have to be causing the problem because I know what im doing when it comes to prepping my grain.  Has anybody else had a problems like these?  This is the second syringe in a row that Ive done "research" with and there is absolutely no signs of viability.  The one has been three weeks and the other is two and there is no indication of viability.  This is the first time using my newly made lids.  The lids are made exactly to Noobies lid tek  specifications. The injection ports are from mad season and the synthetic filter discs are from mycosupply, the filter discs were rtv'd over a half inch hole so I know there's gas exchange.  So its either the syringes or the lids. Has anyone had problems like these with the syringes or lids of this type. 
 
Thanks Fellow shroomites


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"Lets get down to brass tacks, how much for the ape?"

Everything I say on this forum is completly theorectical. A complete fictionilization.

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InvisibleLuger0815
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Re: IS IT THE SYRINGES OR THE LIDS [Re: JonnyLongboard]
    #14415420 - 05/07/11 02:08 PM (12 years, 9 months ago)

.

Edited by Luger0815 (07/04/11 11:16 AM)

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OfflineJonnyLongboard
Habadasher

Registered: 04/19/10
Posts: 126
Loc: Yeti compound
Last seen: 11 years, 1 month
Re: IS IT THE SYRINGES OR THE LIDS [Re: Luger0815]
    #14415586 - 05/07/11 02:43 PM (12 years, 9 months ago)

Its not my grain prep.  I've definitley got that part down pat.  I really dont see how it could be the lids either,  its just thats the only thing different than the last time I did it.  So it has to be the syringes.


--------------------
"Lets get down to brass tacks, how much for the ape?"

Everything I say on this forum is completly theorectical. A complete fictionilization.

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InvisibleBoozie
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Re: IS IT THE SYRINGES OR THE LIDS [Re: JonnyLongboard]
    #14415656 - 05/07/11 02:58 PM (12 years, 9 months ago)

Yea, even if you screwed up on the lids, i'd imagine you'd at least see something.

What teks are you following? Or what's your method from grain prep to inoculation? There's gotta be some holes somewhere along the line. I'm not trying to say you're wrong or anything, but I also find it hard to believe that Sporeworks would send out 2 bunk syringes! I've used them for a couple years now and i've never been anything but satisfied.


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"After silence, that which comes nearest to expressing the inexpressible is music." - Aldous Huxley


   

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OfflineAdam553
workin' on it...
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Registered: 11/23/09
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Re: IS IT THE SYRINGES OR THE LIDS [Re: Boozie]
    #14415679 - 05/07/11 03:04 PM (12 years, 9 months ago)

sure fire way to see if its the spores, do 2-3 LC's and inject with a little bit, if nothing grows, my guess is its bunk. but make sure your procedure is right. theres nothing worse than someone blaming a company when they can't get their shit right.


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The List: Caffeine, Nicotine, Morphine, Codine, Dilaudid, Amphetamine, Psilocybin, LSA, Alcohol, Salvia Divinorum, Nitrous

To Do List: Mescaline, DMT, LSD, MAOI, Yopo, MDA

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InvisiblePinsWellWithOthers
Thread Derailer

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Registered: 10/15/10
Posts: 1,834
Re: IS IT THE SYRINGES OR THE LIDS [Re: Adam553]
    #14415897 - 05/07/11 03:52 PM (12 years, 9 months ago)

Clearly the problem is your using microscopy samples for cultivation use. Next time you should select a sample of spores for cultivation instead of microscopic study. There is also a slight chance that it could have been something entirely different. Spores>Agar will save you a lot of guess work if your are able to do it. do it.

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OfflineSomeGuy
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Re: IS IT THE SYRINGES OR THE LIDS [Re: PinsWellWithOthers]
    #14415935 - 05/07/11 03:58 PM (12 years, 9 months ago)

Quote:

PinsWellWithOthers said:
Clearly the problem is your using microscopy samples for cultivation use. Next time you should select a sample of spores for cultivation instead of microscopic study. There is also a slight chance that it could have been something entirely different. Spores>Agar will save you a lot of guess work if your are able to do it. do it.


:picard: Are you letting the jars cool sufficiently?

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Offlinesporesmores420
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Re: IS IT THE SYRINGES OR THE LIDS [Re: SomeGuy]
    #14416188 - 05/07/11 05:05 PM (12 years, 9 months ago)

What size of jars are you using?????


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Invisiblelinkamathingy
Aspiring Mycologist


Registered: 10/27/10
Posts: 1,235
Re: IS IT THE SYRINGES OR THE LIDS [Re: sporesmores420]
    #14416566 - 05/07/11 08:21 PM (12 years, 9 months ago)

ahem, i believe it is, uh, brass tacks. and probably the lids


--------------------
SCIENCE!!!
If NIST didn't even investigate whether explosives were used, how can we trust their investigation? It's a rule whenever explosions are heard.
Though I Laugh
EyegasmArt.com
anonymous: without name
Anonymous: a group with a name
don't be fooled, have a revolution on your own terms.

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OfflineJonnyLongboard
Habadasher

Registered: 04/19/10
Posts: 126
Loc: Yeti compound
Last seen: 11 years, 1 month
Re: IS IT THE SYRINGES OR THE LIDS [Re: Boozie]
    #14416594 - 05/07/11 09:11 PM (12 years, 9 months ago)

Quote:

Boozie said:
Yea, even if you screwed up on the lids, i'd imagine you'd at least see something.

What teks are you following? Or what's your method from grain prep to inoculation? There's gotta be some holes somewhere along the line. I'm not trying to say you're wrong or anything, but I also find it hard to believe that Sporeworks would send out 2 bunk syringes! I've used them for a couple years now and i've never been anything but satisfied.




I know it sounds crazy to say both syringes are bunk but I did not change my grain prep method from how ive done it before and its worked just fine.  I rinse the grain with hot water very very well.  Then soak for 12 to 24 hours (preferably closer to 12) then rinse again.  Place back in the same pot it soaked in then cover with plenty of water.  Bring to boil and keep it there for 5 to 10 min(closer to 5).  Pour the steaming hot grain into colander to let in drain and steam the exterior moisture off.  Toss every few minutes for twenty minutes.  Load into jars.  Cover with foil.  Pressure cook for 90 mins.  Allow to cool completly.  Open pressure cooker, remove foil.  Give a little shake.  Now its time to shoot them up.  Two cc's per quart jar.  Place jars into incubator at 78 to 80 degrees.  Well thats my grain prep and thats exactly how I did these jars.  I know that this is good prep Ive done my studying.  That is why I belive it to be the syringes.


--------------------
"Lets get down to brass tacks, how much for the ape?"

Everything I say on this forum is completly theorectical. A complete fictionilization.

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OfflineJonnyLongboard
Habadasher

Registered: 04/19/10
Posts: 126
Loc: Yeti compound
Last seen: 11 years, 1 month
Re: IS IT THE SYRINGES OR THE LIDS [Re: Adam553]
    #14416607 - 05/07/11 09:16 PM (12 years, 9 months ago)

Quote:

Adam553 said:
sure fire way to see if its the spores, do 2-3 LC's and inject with a little bit, if nothing grows, my guess is its bunk. but make sure your procedure is right. theres nothing worse than someone blaming a company when they can't get their shit right.




I have my grain prep method down pat.  Its not like I want to give them bad press.  In fact if you search my threads you will see that I gave them an eccellent write up upon receiving my spores.


--------------------
"Lets get down to brass tacks, how much for the ape?"

Everything I say on this forum is completly theorectical. A complete fictionilization.

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OfflineJonnyLongboard
Habadasher

Registered: 04/19/10
Posts: 126
Loc: Yeti compound
Last seen: 11 years, 1 month
Re: IS IT THE SYRINGES OR THE LIDS [Re: SomeGuy]
    #14416614 - 05/07/11 09:18 PM (12 years, 9 months ago)

Quote:

paducahovoids said:
Quote:

PinsWellWithOthers said:
Clearly the problem is your using microscopy samples for cultivation use. Next time you should select a sample of spores for cultivation instead of microscopic study. There is also a slight chance that it could have been something entirely different. Spores>Agar will save you a lot of guess work if your are able to do it. do it.


:picard: Are you letting the jars cool sufficiently?





yes the jars are allowed to cool completly before inoculation.


--------------------
"Lets get down to brass tacks, how much for the ape?"

Everything I say on this forum is completly theorectical. A complete fictionilization.

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OfflineJonnyLongboard
Habadasher

Registered: 04/19/10
Posts: 126
Loc: Yeti compound
Last seen: 11 years, 1 month
Re: IS IT THE SYRINGES OR THE LIDS [Re: linkamathingy]
    #14416649 - 05/07/11 09:26 PM (12 years, 9 months ago)

Quote:

linkamathingy said:
ahem, i believe it is, uh, brass tacks. and probably the lids




Yes I know all about the soilders shoes being wore to the brass tacks.  Im using a quote by Hunter S. Thompson  from Fear and Loathing in Las Vegas.  How could it be the lids.  They have half inch hole covered by a synthetic filter disc so there is definetly gas exchange. Why do you say its the lids?


--------------------
"Lets get down to brass tacks, how much for the ape?"

Everything I say on this forum is completly theorectical. A complete fictionilization.

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Invisiblelinkamathingy
Aspiring Mycologist


Registered: 10/27/10
Posts: 1,235
Re: IS IT THE SYRINGES OR THE LIDS [Re: JonnyLongboard]
    #14416659 - 05/07/11 09:27 PM (12 years, 9 months ago)

"Clearly the problem is your using microscopy samples for cultivation use. Next time you should select a sample of spores for cultivation instead of microscopic study. There is also a slight chance that it could have been something entirely different. Spores>Agar will save you a lot of guess work if your are able to do it. do it."

did you buy the research labeled syringes? this is the best reason i can see on this thread.

sorry about that, i really just wanted to point out the tacks thing. imma go check that quote, do you know the page?


--------------------
SCIENCE!!!
If NIST didn't even investigate whether explosives were used, how can we trust their investigation? It's a rule whenever explosions are heard.
Though I Laugh
EyegasmArt.com
anonymous: without name
Anonymous: a group with a name
don't be fooled, have a revolution on your own terms.

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OfflineJonnyLongboard
Habadasher

Registered: 04/19/10
Posts: 126
Loc: Yeti compound
Last seen: 11 years, 1 month
Re: IS IT THE SYRINGES OR THE LIDS [Re: linkamathingy]
    #14416691 - 05/07/11 09:33 PM (12 years, 9 months ago)

Quote:

linkamathingy said:
"Clearly the problem is your using microscopy samples for cultivation use. Next time you should select a sample of spores for cultivation instead of microscopic study. There is also a slight chance that it could have been something entirely different. Spores>Agar will save you a lot of guess work if your are able to do it. do it."

did you buy the research labeled syringes? this is the best reason i can see on this thread.

sorry about that, i really just wanted to point out the tacks thing. imma go check that quote, do you know the page?




Its been a long time since I saw the movie and even longer since I read the book.  But its probably something that they added to the movie for entertainment vaule.  But mabey not.  Im really not sure.


--------------------
"Lets get down to brass tacks, how much for the ape?"

Everything I say on this forum is completly theorectical. A complete fictionilization.

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Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: IS IT THE SYRINGES OR THE LIDS [Re: JonnyLongboard]
    #14416747 - 05/07/11 09:45 PM (12 years, 9 months ago)

I've had several sporeworks spores that werent worth a shit. I got my mexicana print from them, it was absolutely worthless. 4 little prints on a square of foil that were so faint you couldnt even see them unless you turned it just right in the light. Im talking ridiculously light. It wouldnt have been a big deal if I could have gotten them to germinate on agar...But nope they would never germinate. Then there was my PEU syringe, hopelessly contaminated with what I guess is bacteria. Then there was the APE syringe, same thing. I used to have good luck with Sporeworks, but for the last year or so they have been worthless to me.


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

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InvisibleLuger0815
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Re: IS IT THE SYRINGES OR THE LIDS [Re: sandman420]
    #14417432 - 05/07/11 11:55 PM (12 years, 9 months ago)

Did you use organic rye?
Can you upload a pic. of the jars?

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Offlineskiddy
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Registered: 03/25/11
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Re: IS IT THE SYRINGES OR THE LIDS [Re: Luger0815]
    #14417582 - 05/08/11 12:42 AM (12 years, 9 months ago)

OK first saying he bought the wrong spores labled for microscopy  is bullshit, there labeled for microscopy use so there able to sell them legally.... they should be viable under this label .


--------------------
PESH : Pinning
Transeski : colonizing
Orrisa : colonizing
Mex a : colonizing

You're not a mycologist just because you grow mushrooms.

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Invisibleslapphappypill
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Re: IS IT THE SYRINGES OR THE LIDS [Re: skiddy]
    #14417617 - 05/08/11 12:51 AM (12 years, 9 months ago)

Quote:

skiddy said:
OK first saying he bought the wrong spores labled for microscopy  is bullshit, there labeled for microscopy use so there able to sell them legally.... they should be viable under this label .



yeah, dude is a tool for even saying that... I'd leave a rating for him but not worth the effort :shrug:


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~

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Offlineskiddy
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Re: IS IT THE SYRINGES OR THE LIDS [Re: slapphappypill]
    #14417678 - 05/08/11 01:07 AM (12 years, 9 months ago)

Yeah it wont help :smile:


--------------------
PESH : Pinning
Transeski : colonizing
Orrisa : colonizing
Mex a : colonizing

You're not a mycologist just because you grow mushrooms.

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