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OfflineEnterTheMoon
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Registered: 02/26/11
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First Spore Prints / Syringes
    #14413861 - 05/07/11 05:15 AM (12 years, 8 months ago)

OK i've taken some spoe prints and i'm planning on making a spore syringe from them in the next couple of days, I have a few questions.

Is it completely necessary to sterilize the paper that the spore print is on? can I get away with it if I haven't done this? because I have just placed 4 mushroom caps onto some A4 paper that was in a pack in my room and let the spores drop onto that.

Also, is it ok for me to re-use the syringe that the spores of my grow came in from the shop? How can I sterilise a syringe that has already been used? would sucking up boiled water into the syringe and then ejecting and sucking up more boiled water, then flame sterilizing the needle suffice?

My third question is, how shall I sterilize my jar that I use to scrape the spores into from the print? the same way as I did with my jars before I inoculated them?

Cheers


--------------------


Everything under the sun is in tune, but the sun is eclipsed by the moon.


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Offline3n1gm4
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Re: First Spore Prints / Syringes [Re: EnterTheMoon]
    #14413911 - 05/07/11 05:39 AM (12 years, 8 months ago)

You can reuse a spore needle and sterilize it by sucking up boiling water 4 times and keeping the water in it and allowing it to cool in the syringe with the cap on. To properly sterilize it you need to take it apart and put it in a jar of water and PC it at 15 PSI. I have had success more than once sterilizing with boiling water though.

It is funny because I am in the same process, I just sterilized a used spore needle I have used many times with boiling water, I left the water in on the fifth or sixth pull and let it cool before ejecting the water that isn't needed because I am only nocing one grain jar to make a GLC with for making 10-12 more rye jars. I also took my needle apart and cleaned it with alcohol first to get stray strands of myc that were stuck in it from a LC out b4 I did the boiling water.

One difference in my method is that I am going to expel a few cc of the sterile water onto my print, sterilize a safety pin with fire, and use the rounded part of the safety pin to stir the water I expelled onto the print untill it is thick with spores and then suck the spore water back into the syringe and shake it b4 nocing my rye jar.

BTW boiled water isn't really sterile, if you want to do it the sterile way you will need to PC the syringe and a jar of water for making spore solution for 90 minutes at 15 PSI.:goodluck:


--------------------
http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!


Edited by 3n1gm4 (05/07/11 05:43 AM)


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InvisibleUnnamedGrower
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Re: First Spore Prints / Syringes [Re: EnterTheMoon]
    #14413938 - 05/07/11 05:57 AM (12 years, 8 months ago)

Quote:

EnterTheMoon said:
OK i've taken some spoe prints and i'm planning on making a spore syringe from them in the next couple of days, I have a few questions.

Is it completely necessary to sterilize the paper that the spore print is on? can I get away with it if I haven't done this? because I have just placed 4 mushroom caps onto some A4 paper that was in a pack in my room and let the spores drop onto that.

Also, is it ok for me to re-use the syringe that the spores of my grow came in from the shop? How can I sterilise a syringe that has already been used? would sucking up boiled water into the syringe and then ejecting and sucking up more boiled water, then flame sterilizing the needle suffice?

My third question is, how shall I sterilize my jar that I use to scrape the spores into from the print? the same way as I did with my jars before I inoculated them?

Cheers



Aluminum foil works way better for prints.  I just spray it down with lysol/bleach solution wipe it and lay the caps on.  U probably wont have any problems with the paper from the pack.

Ive never had a contam by boiling water and ejecting it 3-4 times and letting the water sit.  The dish that u are gonna use(i like shot glasses) will be fine washed and then wiped down with an alchol pad or sprayed with lysol/bleach solution

KISS


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Offlineveda_sticks
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Re: First Spore Prints / Syringes [Re: 3n1gm4]
    #14414359 - 05/07/11 09:21 AM (12 years, 8 months ago)

Quote:

UnnamedGrower said:
Quote:

EnterTheMoon said:
OK i've taken some spoe prints and i'm planning on making a spore syringe from them in the next couple of days, I have a few questions.

Is it completely necessary to sterilize the paper that the spore print is on? can I get away with it if I haven't done this? because I have just placed 4 mushroom caps onto some A4 paper that was in a pack in my room and let the spores drop onto that.

Also, is it ok for me to re-use the syringe that the spores of my grow came in from the shop? How can I sterilise a syringe that has already been used? would sucking up boiled water into the syringe and then ejecting and sucking up more boiled water, then flame sterilizing the needle suffice?

My third question is, how shall I sterilize my jar that I use to scrape the spores into from the print? the same way as I did with my jars before I inoculated them?

Cheers



Aluminum foil works way better for prints.  I just spray it down with lysol/bleach solution wipe it and lay the caps on.  U probably wont have any problems with the paper from the pack.

Ive never had a contam by boiling water and ejecting it 3-4 times and letting the water sit.  The dish that u are gonna use(i like shot glasses) will be fine washed and then wiped down with an alchol pad or sprayed with lysol/bleach solution

KISS





Thats not really needed for foil. The manufacturing process pretty much sterilises it. Just to be safe discarding the first meter, then all thats needed is to wipe it down with IPA before placing your cap on the foil inisde a glovebox. This is all i have done when making prints and never had any problems.
Quote:

3n1gm4 said:
You can reuse a spore needle and sterilize it by sucking up boiling water 4 times and keeping the water in it and allowing it to cool in the syringe with the cap on. To properly sterilize it you need to take it apart and put it in a jar of water and PC it at 15 PSI. I have had success more than once sterilizing with boiling water though.

It is funny because I am in the same process, I just sterilized a used spore needle I have used many times with boiling water, I left the water in on the fifth or sixth pull and let it cool before ejecting the water that isn't needed because I am only nocing one grain jar to make a GLC with for making 10-12 more rye jars. I also took my needle apart and cleaned it with alcohol first to get stray strands of myc that were stuck in it from a LC out b4 I did the boiling water.

One difference in my method is that I am going to expel a few cc of the sterile water onto my print, sterilize a safety pin with fire, and use the rounded part of the safety pin to stir the water I expelled onto the print untill it is thick with spores and then suck the spore water back into the syringe and shake it b4 nocing my rye jar.

BTW boiled water isn't really sterile, if you want to do it the sterile way you will need to PC the syringe and a jar of water for making spore solution for 90 minutes at 15 PSI.:goodluck:




dont over use spores. you only need a small part of a print to make up a spore syringe. the best spore syringes IMO is a one that looks just like water.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Offline3n1gm4
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Re: First Spore Prints / Syringes [Re: veda_sticks]
    #14414389 - 05/07/11 09:32 AM (12 years, 8 months ago)

Quote:

veda_sticks said:

dont over use spores. you only need a small part of a print to make up a spore syringe. the best spore syringes IMO is a one that looks just like water.




Why is that? I thought the more spores the better, is there a disadvantage to using too many spores in a syringe besides wasting spores?


--------------------
http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!


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InvisibleUnnamedGrower
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Re: First Spore Prints / Syringes [Re: 3n1gm4]
    #14414407 - 05/07/11 09:37 AM (12 years, 8 months ago)

Quote:

3n1gm4 said:
Quote:

veda_sticks said:

dont over use spores. you only need a small part of a print to make up a spore syringe. the best spore syringes IMO is a one that looks just like water.




Why is that? I thought the more spores the better, is there a disadvantage to using too many spores in a syringe besides wasting spores?



Yes the less spores means less genetic competition for an MS syringe.


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Offline3n1gm4
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Re: First Spore Prints / Syringes [Re: UnnamedGrower]
    #14414468 - 05/07/11 09:59 AM (12 years, 8 months ago)

Ahh, I see said the blind man. Thanks! I was going to get as many spores as I could in there, TYVM, maybe my pinset will be better that it would have been with a million spores.


--------------------
http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!


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OfflinePuzzled
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Registered: 03/28/11
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Re: First Spore Prints / Syringes [Re: veda_sticks]
    #14414475 - 05/07/11 10:04 AM (12 years, 8 months ago)

Quote:

veda_sticks said:
Thats not really needed for foil. The manufacturing process pretty much sterilises it.




And here I've been sterilizing my foil in the oven.  Thanks for the info, it will save me a step.


--------------------
Everything I say is just general thoughts and should not be taken as advice, recommendations, or suggestions.  If I write "you," it's as an indefinite personal pronoun because I find the use of "one" too formal and tiresome.


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OfflineOregonChronic
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Re: First Spore Prints / Syringes [Re: EnterTheMoon]
    #14414555 - 05/07/11 10:34 AM (12 years, 8 months ago)

I had success with printing off my 3rd flush from my 1st gro of B+. With clean washed hands and without touching the cap...and i picked the chosen shroom by its stem base and quickly popped the lid off a brand new unopened canning jar without sterilizing it :shrug: and carefuly removed the cap from the stem with the tip of an iso'd knife and let it drop in the jar and i just put the lid on the jar without its sealing ring and let drop spores all day.

The funny thing too,...is i put the cap in the jar while working in open air :lol: and the cap landed upside down in the jar when i attempted to cut it from the stem and let drop so i had to sit there repeatedly trying to flip it gills side down. :facepalm::lol:

Then i just sucked up boiling water into a 12 cc syringe a few good times and let sit and sterilize a few times and let it cool on the last pull of boiling water and put it inside my ghetto "glovebox" with an iso'd knife and the jar with the print.


I just scraped the spores around in the jar with the sterile knife and squirted the 12cc of sterile water on the spores and stired up a bit more,..sucked it all back in slowly,...then squirted it all back out into the jar and stired again and sucked all 12cc back into the syringe,...flamed the needle, let cool, and capped it.

I inocd 5 cakes with that syringe and only 1 got contamd at the top by the dry verm layer with green and thats because i inocd em in open air. The other 4 are on their consolidation stage and are about to be birthed soon because one has an in vitro pin and they are looking really rhizo.


--------------------
:mushroomgrow:Wavy Cap Cyan Spore Prints for Trade/Sale :mushroomgrow:



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InvisibleUnnamedGrower
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Re: First Spore Prints / Syringes [Re: OregonChronic]
    #14414570 - 05/07/11 10:39 AM (12 years, 8 months ago)

Quote:

OregonChronic said:


I just scraped the spores around in the jar with the sterile knife and squirted the 12cc of sterile water on the spores and stired up a bit more,..sucked it all back in slowly,...then squirted it all back out into the jar and stired again and sucked all 12cc back into the syringe,...flamed the needle, let cool, and capped it.





I would have put 500-600 ml of water and made 50-60 syringes from this print.  One syringe out of a print is ridiculous IMHO:shrug:


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OfflineEnterTheMoon
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Re: First Spore Prints / Syringes [Re: UnnamedGrower]
    #14429489 - 05/10/11 11:55 AM (12 years, 8 months ago)

ok thanks for the info, i was also going to put as many spore prints into my syringe as i could, theres a video on youtube that says to use 4 per jar! can someone explain why it is worse to use a lot of prints rather than just one? what do you mean by less competition for an MS Syringe?

I have about 5 pore prints and two syringes, hmmmmmmm


--------------------


Everything under the sun is in tune, but the sun is eclipsed by the moon.


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Offline3n1gm4
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Re: First Spore Prints / Syringes [Re: EnterTheMoon]
    #14429514 - 05/10/11 12:01 PM (12 years, 8 months ago)

Multi spore inoculations have many genetics competing with each other, the more spores you use the more genetics there are fighting to take hold. That is my understanding of it anyway.


--------------------
http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!


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InvisibleSillicybin
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Re: First Spore Prints / Syringes [Re: 3n1gm4]
    #14429671 - 05/10/11 12:37 PM (12 years, 8 months ago)

Foil > Paper

The spores won't want to stick to the foil as badly when you go to scrape the print into water.  They tend to want to stick to paper.  Also if the paper gets wet/moist from the cap, there is a small chance that some of the spores will germinate on paper.

Use a little Jet-Dry or water-based lube to prevent spore clumping in syringes.


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