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Caine
lab rat



Registered: 01/25/10
Posts: 3,920
Loc: NE
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Re: Peptide psychedelics? [Re: The Whale]
#14411447 - 05/06/11 05:26 PM (12 years, 9 months ago) |
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Quote:
The Whale said: From the abstract: "The results show that the model can be used in drug discovery for virtual screening and structure-based ligand design as well as in GPCR activation studies." Interesting.
You would have more success posting this at Bluelight. There are informed chemists afoot.
I dunno if informed chemists are what I'm looking for, usually they can speak for hours on end about various organic chem techniques and are very handy when you want a small molecule in a pinch but peptide synthesis is a whole different ballgame.
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mellowparty
legitimate researcher


Registered: 05/17/09
Posts: 18,467
Last seen: 10 years, 3 months
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Re: Peptide psychedelics? [Re: Beanhead]
#14411458 - 05/06/11 05:30 PM (12 years, 9 months ago) |
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Quote:
Beanhead said: You'll discover more reading this...
neat
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Caine
lab rat



Registered: 01/25/10
Posts: 3,920
Loc: NE
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Quote:
mellowparty said:
Quote:
Beanhead said: You'll discover more reading this...
neat
I downloaded it, I'll start the reading before I go to sleep later, looks really interesting. I made a thread in here maybe 3 or 4 months ago about omega conotoxin, which is a peptide in a certain snail's venom that's on the order of 1000 times more potent than morphine. Peptides seem to be really promising in the medical field, I don't know why there aren't more studies being done on this stuff.
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badchad
Mad Scientist

Registered: 03/02/05
Posts: 13,374
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If you got too large, you may not fit the receptor.
It may depend on how the peptide bond affects the final conformation of the protein.
-------------------- ...the whole experience is (and is as) a profound piece of knowledge. It is an indellible experience; it is forever known. I have known myself in a way I doubt I would have ever occurred except as it did. Smith, P. Bull. Menninger Clinic (1959) 23:20-27; p. 27. ...most subjects find the experience valuable, some find it frightening, and many say that is it uniquely lovely. Osmond, H. Annals, NY Acad Science (1957) 66:418-434; p.436
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Caine
lab rat



Registered: 01/25/10
Posts: 3,920
Loc: NE
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Re: Peptide psychedelics? [Re: badchad]
#14411497 - 05/06/11 05:41 PM (12 years, 9 months ago) |
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Quote:
badchad said: If you got too large, you may not fit the receptor.
It may depend on how the peptide bond affects the final conformation of the protein.
True, that's what would be so difficult about its synthesis, but in theory there is nothing wrong with it. That's partially why (bio) was in the OP, because I know that botox has taken ages of evolution to be created. It would be crazy to be bitten by some animal only to find yourself tripping absolute balls ten minutes later to the point of immobilization. Crazy defense mechanism, I tell you what
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Beanhead
IS IRONIC PARADOX


Registered: 10/11/08
Posts: 17,257
Loc: Geospatial inversion.
Last seen: 3 years, 6 months
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Re: Peptide psychedelics? [Re: Caine]
#14413788 - 05/07/11 04:15 AM (12 years, 9 months ago) |
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Handbook of Biologically Active Peptides
EDIT: Abba Kastin Abba Kastin, Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA
Number Of Pages: 1640 Publication Date: 2006-08-29 Sales Rank: 990838 ISBN / ASIN: 0123694426 EAN: 9780123694423 Binding: Hardcover Book
Description: Peptides play a crucial role in many physiological processes including actions as neurotransmitters, hormones, and antibiotics. Research has shown their importance in such fields as neuroscience, immunology, pharmacology, and cell biology. The Handbook of Biologically Active Peptides presents, for the first time, this tremendous body of knowledge in the field of biologically active peptides in one single reference. The section editors and contributors represent some of the most sophisticated and distinguished scientists working in basic sciences and clinical medicine. The Handbook of Biologically Active Peptides is a definitive, all-encompassing reference that will be indispensable for individuals ranging from peptide researchers, to biochemists, cell and molecular biologists, neuroscientists, pharmacologists, and to endocrinologists. Chapters are designed to be a source for workers in the field and will enable researchers working in a specific area to examine other related areas with which they would not ordinarily be familiar.*Chapters are designed to be a source for workers in the field and will enable researchers working in a specific area to examine other related areas that they would not ordinarily be familiar.*Fascinating relationships described in the book include the presence of some peptides originally found in frog skin that persist in the human human and brain where they can affect food intake and obesity.
If I get my hands on this i'll put the link up but haven't found it yet, i'm glad i'm non the only one interested in peptides. Althrough my pharmacology/chemistry knowledge isn't sufficient most of the time to understand everything, it's a gread read nontheless (I can register for chemistry education tommorow  FINALLY )
Edited by Beanhead (05/07/11 04:24 AM)
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Caine
lab rat



Registered: 01/25/10
Posts: 3,920
Loc: NE
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Re: Peptide psychedelics? [Re: Beanhead]
#14413916 - 05/07/11 05:41 AM (12 years, 9 months ago) |
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Beanhead, you should try to do some research on possible peptide psychedelics once you have access to some good lab equipment and protein writing tools
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Beanhead
IS IRONIC PARADOX


Registered: 10/11/08
Posts: 17,257
Loc: Geospatial inversion.
Last seen: 3 years, 6 months
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Re: Peptide psychedelics? [Re: Caine]
#14413933 - 05/07/11 05:53 AM (12 years, 9 months ago) |
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Who knows?
I like chemistry, there is already so much research done about it that you can't possibly grasp everything... And then you realize we know barely anything at all . Lovely !
h**p://ifile.it/9j8zxy/ebooksclub.org__Handbook_of_Biologically_Active_Peptides.pdf
Edited by Beanhead (05/07/11 05:55 AM)
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Spiderbaby
?



Registered: 08/20/06
Posts: 1,439
Loc: Ireland
Last seen: 3 years, 11 months
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Re: Peptide psychedelics? [Re: Caine]
#14414279 - 05/07/11 08:53 AM (12 years, 9 months ago) |
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Psychoactive peptides are known, Dermorphin for example, its unusual as its sequence contains D-Alanine.
Unfortunately developing small peptide drugs would be difficult since small peptides can adopt a large number of conformations compared to a small organic molecules which have a well defined shape to "fit" a receptor. And add to that the difficulty or near impossibility of solving structures of integral membrane proteins like GPCRs. Structure based drug design would be extremely difficult for peptide drugs. The design approach would have to be assaying vast libraries of peptides for activity and then optimizing from there once you have a lead peptide.
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Mad_Larkin

Registered: 11/29/07
Posts: 18,606
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Re: Peptide psychedelics? [Re: Spiderbaby]
#14414320 - 05/07/11 09:08 AM (12 years, 9 months ago) |
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Interesting shit. That dermorphin stuff is a constituent of the frog venom South American indians use when they go hunting.
Quote:
Dermorphin is about 30-40 times more potent than morphine but less likely to produce drug tolerance and addiction.
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Spiderbaby
?



Registered: 08/20/06
Posts: 1,439
Loc: Ireland
Last seen: 3 years, 11 months
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Re: Peptide psychedelics? [Re: Mad_Larkin]
#14414358 - 05/07/11 09:21 AM (12 years, 9 months ago) |
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i think they call it sapo or something,
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mellowparty
legitimate researcher


Registered: 05/17/09
Posts: 18,467
Last seen: 10 years, 3 months
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Re: Peptide psychedelics? [Re: Spiderbaby]
#14953599 - 08/20/11 06:14 PM (12 years, 6 months ago) |
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Quote:
Spiderbaby said: Psychoactive peptides are known, Dermorphin for example, its unusual as its sequence contains D-Alanine.
What if you clone the nucleotide sequence of this peptide (corrected for codon bias in humans) and also put in an amino acid isomerase gene to give the enzyme that will create the geometrically unusual kink in the polypeptide backbone. Then stick a rabies virus promoter element and just for fun attach an N-terminal secretion signal. That last bit may perturb the properties of such a small and unusual peptide and probably the isomerase would have to be targeted to the endoplasmic reticulum 
So assuming you have your psychoactive peptide on a plasmid with all the molecular requirements for it to be active at the correct site (the synaptic interface). Then you inject yourself in the middle of the brain and subject your head to mediolateral electrostatic field so that the plasmid is electroporated towards the anode (which you point to the brain hemisphere of choice). The plasmid gets expressed and secreted and binds your receptor of choice. You trip ballz or die of a respiratory arrest 
In order to ensure the competence of the cells you can put your construct in a retrovirus or better yet in exosomes with recombinant surface proteins (since we said rabies then rabies virus glycoprotein that will bind acetylcholinergic neurons)
I just got back from work and I'm pretty baked atm so I might have made a mistake somewhere. If its correct then its gonna be a hell of a trip.
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