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InvisibleZildjian
human

Registered: 09/11/02
Posts: 208
Loc: new zealand
Wild shaggy mane syringe
    #1440885 - 04/08/03 11:27 PM (14 years, 7 months ago)

Hello, I found a shaggy mane while lurking around in the woods the other day. I thought to myself "hmmmmm, maybe I could get some spores out of this bugger and grow me some shaggy manes!" So I pulled it out of the ground and took it home. The shaggy mane was in the digesting itself stage and was dripping spore juice everywhere, I decided to submerge the cap in some boiled water and mix it around to make a kind of spore solution. The result was a black liquid which i sucked into a syringe and used to innoculate some agar plates. I figured that the plates would indefinately contam, but perhaps i could take out any mycelium that germinated and run with it. I was wondering if perhaps the mass of spores would overrun any contam, as i've seen happen a couple times with oysters. Has anyone made a syringe from a shaggy mane before? What happened? any suggestions?


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Offlinezeronio
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Registered: 10/16/01
Posts: 2,349
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Re: Wild shaggy mane syringe [Re: Zildjian]
    #1441740 - 04/09/03 04:34 AM (14 years, 7 months ago)

I heard that it's hard to get a clean culture like this - but let us know! I'm looking forward to see the results. Good luck!


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Offliner05c03
The Slug Scourge
Registered: 01/06/02
Posts: 383
Loc: Indiana, US
Last seen: 12 years, 7 months
Re: Wild shaggy mane syringe [Re: Zildjian]
    #1442124 - 04/09/03 09:09 AM (14 years, 7 months ago)

Never tried it, I have just cloned tissue from fresh ones. I would say bacterial contams are going to be a problem. Do you have access to antibiotics? That would help alot. Another thing that you can try is to make some water agar (water + agar), lower the pH to about 5 and try to germinate the spores on that. Bacteria do not like the low nutrient water agar and they do not like the low pH (in general). Good luck.


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InvisibleZildjian
human

Registered: 09/11/02
Posts: 208
Loc: new zealand
Re: Wild shaggy mane syringe [Re: Zildjian]
    #1463139 - 04/16/03 08:49 AM (14 years, 7 months ago)

unfortunately, the plates contraminated way before any mycelium could grow, so I decided to water the thing down to 1.5 litres and innoculate my backyard, hopefully i might see some shrooms next year. Found two shaggy parasols the other day, one with a cap around 10cm across which i made a spore print from, and a smaller pin which i might go back and pick in a few days. I'm going to make some more agar plates tomorrow and innoculate them with some of this print. I'll tell you how it goes.


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Offlinezeronio
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Registered: 10/16/01
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Re: Wild shaggy mane syringe [Re: Zildjian]
    #1469217 - 04/18/03 03:32 AM (14 years, 7 months ago)

Shaggy manes are very easy to clone from the center of the stem. I never tried to germinate the spores, but maybe it would be better to dry the slurry and then streak dry spores on agar.


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Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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