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Sharpstuff

Registered: 05/04/11
Posts: 510
Loc: Australia
Last seen: 7 years, 7 months
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LC Help
#14403020 - 05/04/11 11:52 PM (12 years, 8 months ago) |
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Hey my friend just finished his first BRF PF Tek grow, and now hes intrested in LC and Bulk substrate growing.... but he doesn't fully understand how to make a LC
is it simply 1. Create a jar with 96% water 4% Honey. 2. Steralize the jars + let them cool. 3. inject spores from a syringe?? or what im not sure? 4. let it colonize in the liquid? 5. suck it out with fresh sterile syringe and inject it into the bulk substate or watever your growing on?
Thanks if you guys can help =)
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k00laid
NEMO


Registered: 05/03/10
Posts: 19,636
Last seen: 6 months, 5 days
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search for agar's GRAIN LC
-------------------- AMU - AMU Q & A - MyVideo Teks!
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
Loc: The Downside of Up
Last seen: 12 years, 7 months
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Re: LC Help [Re: k00laid]
#14403041 - 05/04/11 11:58 PM (12 years, 8 months ago) |
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You have to have a pressure cooker and you fully colonize a jar of rye. You then either use GTG or make a GLC out of the fully colonized grain jar and make 10 more rye jars from one jar. Really saves on the spores. And it is simple!
http://www.shroomery.org/forums/showflat.php/Number/14366335
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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Sharpstuff

Registered: 05/04/11
Posts: 510
Loc: Australia
Last seen: 7 years, 7 months
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Re: LC Help [Re: 3n1gm4]
#14403101 - 05/05/11 12:11 AM (12 years, 8 months ago) |
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Yes i have a PC, all he wanted to know is their a simple way to do LC, before i spend hours researching.... seems more complex then it should be >.>
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
Loc: The Downside of Up
Last seen: 12 years, 7 months
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1 gram of extra light malt extract to 250ml of water.
Try searching ELME LC if you want a full tek but it is as simple as mixing 1 measured gram (bout a tsp) of ELME to a cup of hot water, disolving it by mixing, then PC it at 15 psi for 30 minutes. That will give you your LC water to noc up.
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
Loc: The Downside of Up
Last seen: 12 years, 7 months
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Re: LC Help [Re: 3n1gm4]
#14403173 - 05/05/11 12:27 AM (12 years, 8 months ago) |
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The key to a good LC is a good lid http://www.shroomery.org/forums/showflat.php?Cat=0&Number=14186384&page=0&vc=1#14186384 you could use sugar in the raw packs for free from starbucks if you want to, as long as there is something for the myc to eat. I like GLCs better than LCs, agars GLC is the best, I can make one rye jar into ten in three weeks total.
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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Sharpstuff

Registered: 05/04/11
Posts: 510
Loc: Australia
Last seen: 7 years, 7 months
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Re: LC Help [Re: 3n1gm4]
#14403205 - 05/05/11 12:35 AM (12 years, 8 months ago) |
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it may sound nooby, but why should i have to read a TEK if its as simple as that?, shouldn't that be all i need to make my firt LC? then go into depth once he learns more about it..?
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Puzzled
Just a guy
Registered: 03/28/11
Posts: 69
Last seen: 12 years, 3 months
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Quote:
Sharpstuff said: it may sound nooby, but why should i have to read a TEK if its as simple as that?, shouldn't that be all i need to make my firt LC? then go into depth once he learns more about it..?
As 3n1gm4 said, you're going to want a good lid. I made my first LC by just using a 1/2 pint jar I used for PF tek with four micropore tape holes. When I pierced one of them with the needle, it left a hole that let in contams (since it didn't have the dry verm layer that keeps the pf jars free). Live and learn. Now I have jars with silicone self-healing injection ports.
Read the tek so you don't make the same sort of mistakes people like me make.
-------------------- Everything I say is just general thoughts and should not be taken as advice, recommendations, or suggestions. If I write "you," it's as an indefinite personal pronoun because I find the use of "one" too formal and tiresome.
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
Loc: The Downside of Up
Last seen: 12 years, 7 months
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Re: LC Help [Re: Puzzled]
#14403244 - 05/05/11 12:48 AM (12 years, 8 months ago) |
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My first LC I used polyfil and a red rtv self healing injection port and it worked fine with no contams. SFDs are awesome but you can succeed without them.
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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Sharpstuff

Registered: 05/04/11
Posts: 510
Loc: Australia
Last seen: 7 years, 7 months
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Re: LC Help [Re: Puzzled]
#14403257 - 05/05/11 12:54 AM (12 years, 8 months ago) |
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Alright, everything you guys have said just doesnt co-operate with my brain, all wanted to know is if eneone can tell me a basic like 4 step guide on how to make a LC... the teks for it are soo much more complicated then like a basic PF TEK mushroom grow,
what would happen if i 1. made a jar with water/honey, and a lid with holes/silocone under them. (ill do math part later) 2. PC the jars, 3. Leave to cool. 4. inject some spores into the Liquid through the siliconed up holes 5. leave it
would this work or do i have to do it x10 more complicated
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
Loc: The Downside of Up
Last seen: 12 years, 7 months
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Elme found at any big liquor or home brew store will be much thinner and clearer than honey, other than that I don't know because I have only bought ELME and it will last me for years.
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Quote:
Sharpstuff said: a basic like 4 step guide on how to make a LC...
1) Mix water with malt 2) inject spores 3) wait 4) fail at mushrooms
LC sucks man, why waste time with failure?
-------------------- You make it all possible. Doesn't it feel good?
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clueless
justnew




Registered: 07/21/03
Posts: 783
Loc: ANE
Last seen: 17 days, 22 hours
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Re: LC Help [Re: 3n1gm4]
#14403903 - 05/05/11 06:57 AM (12 years, 8 months ago) |
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You seem to understand the basics of it, but if you want simple do a grain liquid culture. With the traditional LC you have to grow it out to check viability. With a GLC you start with healthy mycelium.
If you have a clean syringe PC a jar of water. Shake colonized jar a bit, inject water into the jar, shake to mix it up, suck it back up and you are good to go. That's the just of it any way.
Quote:
3n1gm4 said: GLC is the best, I can make one rye jar into ten in three weeks total.
I've used one jar as starter culture and inoculated 14 quart jars with plenty GLC to spare.
-------------------- I'm a rhinestone tiger in a leisure suit.
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ShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~




Registered: 07/18/10
Posts: 8,139
Loc: EVERY WHERE
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Re: LC Help [Re: Doc_T]
#14403937 - 05/05/11 07:14 AM (12 years, 8 months ago) |
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Quote:
Doc_T said:
Quote:
Sharpstuff said: a basic like 4 step guide on how to make a LC...
1) Mix water with malt 2) inject spores 3) wait
4) fail at mushrooms
LC sucks man, why waste time with failure?
5 days LC
 
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emf
#14



Registered: 11/06/10
Posts: 14,756
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Quote:
Sharpstuff said: Hey I just finished my first BRF PF Tek grow, and now I'm intrested in LC and Bulk substrate growing.... but I don't fully understand how to make a LC
is it simply 1. Create a jar with 96% water 4% Honey. 2. Steralize the jars + let them cool. 3. inject spores from a syringe?? or what im not sure? 4. let it colonize in the liquid? 5. suck it out with fresh sterile syringe and inject it into the bulk substate or watever your growing on?
Thanks if you guys can help =)
Fixed. LC's from spore suck. And if you're too lazy to even read a whole tek you will for sure fail at most things mushroom related. Please don't rebuttal with stories of BRF success because that doesn't count.
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
Loc: The Downside of Up
Last seen: 12 years, 7 months
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Looks like the last pic in the second row might have some green separating the top and bottom colonized spots
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
Loc: The Downside of Up
Last seen: 12 years, 7 months
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Quote:
Sharpstuff said:
5. suck it out with fresh sterile syringe and inject it into the bulk substate or watever your growing on?
You can't colonize a substrate with a LC, you have to noc cakes or sterile grain jars with it, then use the grain jars to mix with sub when fully colonized.
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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ShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~




Registered: 07/18/10
Posts: 8,139
Loc: EVERY WHERE
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Re: LC Help [Re: 3n1gm4]
#14403973 - 05/05/11 07:27 AM (12 years, 8 months ago) |
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Quote:
3n1gm4 said: 1 gram of extra light malt extract to 250ml of water.
Try searching ELME LC if you want a full tek but it is as simple as mixing 1 measured gram (bout a tsp) of ELME to a cup of hot water, disolving it by mixing, then PC it at 15 psi for 30 minutes. That will give you your LC water to noc up.
i think most ppl Quote:
3n1gm4 said: Looks like the last pic in the second row might have some green separating the top and bottom colonized spots
no green in these jars yo
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FractalXplora
Grainiack




Registered: 02/11/06
Posts: 2,494
Loc: UK
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Re: LC Help [Re: 3n1gm4]
#14403982 - 05/05/11 07:29 AM (12 years, 8 months ago) |
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i been using stonesuns LC mix, works like a charm and comes out clear.
1 pint water / 3tsp DEX (dextrose) / 1/2 tsp LME (light malt extract)
PC'ed @ 14PSI - 20 mins.
Its a beauty!
try it.
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k00laid
NEMO


Registered: 05/03/10
Posts: 19,636
Last seen: 6 months, 5 days
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Re: LC Help [Re: 3n1gm4]
#14404003 - 05/05/11 07:36 AM (12 years, 8 months ago) |
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Quote:
3n1gm4 said:
Quote:
Sharpstuff said:
5. suck it out with fresh sterile syringe and inject it into the bulk substate or watever your growing on?
You can't colonize a substrate with a LC, you have to noc cakes or sterile grain jars with it, then use the grain jars to mix with sub when fully colonized.
monstermitch uses LC on a sterilized manure substrate. in bags.
it works
-------------------- AMU - AMU Q & A - MyVideo Teks!
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
Loc: The Downside of Up
Last seen: 12 years, 7 months
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Re: LC Help [Re: k00laid]
#14404048 - 05/05/11 07:49 AM (12 years, 8 months ago) |
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Well maybe with poo but that is just because it has all the nutes it needs and that is where it grows anyway. It wouldn't colonize coir, which I assumed he would be using since he said he was new to bulk.
I guess with the right additives to the coir it might colonize, but I am into the easy way, set it and forget it, one and done, get ready for the next batch unless it looks like it will give a good second flush.
I like my sub just slightly below field capacity because it seems to colonize faster, and I like it heavy on the grain spawn. I know and nobody cares. I am just bored soaking grains, guess I will use the hole saw to make a mono out of my new $3 tote.
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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Puzzled
Just a guy
Registered: 03/28/11
Posts: 69
Last seen: 12 years, 3 months
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Re: LC Help [Re: Doc_T]
#14404897 - 05/05/11 11:50 AM (12 years, 8 months ago) |
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Quote:
Doc_T said:LC sucks man, why waste time with failure?
I really respect your knowledge and abilities, and it's great the work you do with agar, but some of us like LCs and get results with which we're happy.
-------------------- Everything I say is just general thoughts and should not be taken as advice, recommendations, or suggestions. If I write "you," it's as an indefinite personal pronoun because I find the use of "one" too formal and tiresome.
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Sillicybin
Registered: 02/14/05
Posts: 2,134
Loc:
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Re: LC Help [Re: 3n1gm4]
#14405205 - 05/05/11 01:14 PM (12 years, 8 months ago) |
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Quote:
3n1gm4 said: Well maybe with poo but that is just because it has all the nutes it needs and that is where it grows anyway. It wouldn't colonize coir, which I assumed he would be using since he said he was new to bulk.
I have gotten a coir bag to successfully colonize using LC. It took forever and it wasn't pretty, but it worked.
That said... coir isn't a great substrate by itself, meaning if you want to get decent results with it, you should use grain spawn. The grain mixed in with the coir gives the mycelium a more diverse substrate. If you just put LC (GLC or otherwise on coir) it is not going to perform very well unless you have a ton of additives.
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