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Offlinearabmobster
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Registered: 04/01/03
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how long can agar be stored after inoculation
    #1439915 - 04/08/03 06:01 PM (14 years, 4 months ago)

how long can agar be stored after inoculation in fridge


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OfflinePooPs
What's thisfor???

Registered: 03/05/03
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Re: how long can agar be stored after inoculation [Re: arabmobster]
    #1439941 - 04/08/03 06:10 PM (14 years, 4 months ago)



This jar was made in january.. inoculated 2 weeks later, used and is still viable today!


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Sniff, Sniff... What's that smell???... ohhhhh.!!
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Pot Free for another : nevermind.. never made it..


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OfflinePennywise
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Registered: 03/21/02
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Re: how long can agar be stored after inoculation [Re: arabmobster]
    #1439945 - 04/08/03 06:14 PM (14 years, 4 months ago)

Quote:

how long can agar be stored after inoculation in fridge


Why do you keep asking this question in peoples posts, plus your post?


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Offlinearabmobster
#1

Registered: 04/01/03
Posts: 317
Loc: Palestine
Last seen: 8 years, 3 months
Re: how long can agar be stored after inoculation [Re: Pennywise]
    #1440031 - 04/08/03 06:48 PM (14 years, 4 months ago)

i just wnted to know
because i just inoculated agar today
and put it in the fridge


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OfflinePooPs
What's thisfor???

Registered: 03/05/03
Posts: 4,510
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Re: how long can agar be stored after inoculation [Re: arabmobster]
    #1440139 - 04/08/03 07:25 PM (14 years, 4 months ago)

If you inoculated it ... incubate it to germinate the spores... they will take forever in teh fridge! !!!!!

Once you use it and want to store it.. then use the fridge...

Dude..


--------------------


-----------
Sniff, Sniff... What's that smell???... ohhhhh.!!
------------------


Pot Free for another : nevermind.. never made it..


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InvisiblePrisoner#1
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Registered: 01/22/03
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Re: how long can agar be stored after inoculation [Re: PooPs]
    #1443415 - 04/09/03 04:30 PM (14 years, 4 months ago)

It can be stored uninoculated and remain usable for at least 7 weeks, after colonization I would say you begin to loose viability after a few months if not stored properly...Pick up the mushroom cultivator by paul stamets, buy it through the shroomery and you get a discount....anyway, Stamets says that some cultures have remained viable for more than 20 years when stored under the right conditions, 2-3 years if it's not....

Prisoner#1


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Offlinemesq
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Re: how long can agar be stored after inoculation [Re: Prisoner#1]
    #1443569 - 04/09/03 05:26 PM (14 years, 4 months ago)

Isn't it best to transfer from a petri to a slant for storage?? A slant being beter because it has more agar for the culture to draw nutrients from whereas a petri is for short term..

This is assuming the original poster is using petris.


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InvisiblePrisoner#1
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Re: how long can agar be stored after inoculation [Re: mesq]
    #1443811 - 04/09/03 06:42 PM (14 years, 4 months ago)

I would have to say....yes...but petris are more for obtaining a pure culture as opposed to any sort of storage, they dont seal well and allow contaminants in...there are ways around this....using a short half pint jar with about half the volume being agar is more than ideal.....but if it's to be any longer than 2 months in a sealed and refridgerated petri then the slant need to be involved....

did that last part sound a little racist?

Prisoner#1


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