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OfflineBlueflowers
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Registered: 03/15/11
Posts: 174
Loc: Australia
Last seen: 7 days, 11 hours
First time inoc am i doing it rite?
    #14398508 - 05/04/11 06:21 AM (12 years, 8 months ago)

Went ahead and PC'd my rye grains today , 15psi for about 75 minuets and about 6 hours to cool down. Though i noticed after there was quite alot of condensation in the jar( no pooling) just the grain sticking to the sides n stuff , is that normal?

anyway i let the jar cool down and inoculated with about 2-3 cc's worth of B+ spore syringe and shook the jar a little. i proceeded to place the jar in a cardboard box with a paper bag on top of it and i though i might leave  one those little packet things you find in chip packets in the box until morning to hopefully get rid of any moisture trying to get in .
So hoping i am successful when do you guys think i will see any sign of colonization?

thoughts and comments would be greatly appreciated

Cheers


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InvisibleCarl Sagan
Time Dilation Analyst


Registered: 04/19/11
Posts: 922
Loc: Myco-tek.org
Re: First time inoc am i doing it rite? [Re: Blueflowers]
    #14398573 - 05/04/11 06:56 AM (12 years, 8 months ago)

1 cc per jar is plenty. Get rid of that damp rid packet. Other than that its all patience.:rockon:


--------------------
“Sacred cows make the best hamburger”

Mark Twain



Independant Research Foundation


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OfflineBlueflowers
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Re: First time inoc am i doing it rite? [Re: Carl Sagan]
    #14398588 - 05/04/11 07:05 AM (12 years, 8 months ago)

damp rid is gawnnn wish me luck , ill post some pics if i see any growth in the next couple of days.... :highdog: crossing fingers


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Offlinear1es
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Re: First time inoc am i doing it rite? [Re: Blueflowers]
    #14403060 - 05/05/11 12:03 AM (12 years, 8 months ago)

how did you prepare your rye

u need to make sure they are dry on the outside before loading your jars

also dont shake jars after inoc with spore solution

takes longer for the spores to do the dirty

1 CC is more than enough spores as well

what are your plans with the rye after they are done ?

u should try doing a g2g if you havent already thought of that


--------------------
":mushroom2:here is nothing lost or wasted in this life.” - Bhagavad Gita


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OfflineBlueflowers
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Registered: 03/15/11
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Re: First time inoc am i doing it rite? [Re: Carl Sagan]
    #14427109 - 05/09/11 09:41 PM (12 years, 8 months ago)

Well it has been 6 days and no growth in my jars , but its also very cold where im at now so i have the heater on very low to see if that will help :/ the upside to this is there is no mold whatsoever which means i must have done my innoc and sterilization properly . but if this doesn't colonize for my next try i might need a incubating chamber(more money outta my wallet,yay) :frown:

btw ar1es my plan was to do g2g , LC culture looks like alot more time investing.


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Offlinear1es
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Re: First time inoc am i doing it rite? [Re: Blueflowers]
    #14428591 - 05/10/11 07:17 AM (12 years, 8 months ago)

a grain LC is pretty simple if you have empty syringes


--------------------
":mushroom2:here is nothing lost or wasted in this life.” - Bhagavad Gita


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OfflineBlueflowers
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Registered: 03/15/11
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Loc: Australia
Last seen: 7 days, 11 hours
Re: First time inoc am i doing it rite? [Re: Carl Sagan]
    #14455088 - 05/15/11 07:15 AM (12 years, 8 months ago)

First growth sign after 12 days ive noticed small white wispy growth from the the grains at the bottom of the jar.  its going pretty slow due to the temperatures dropping down to the low 50's here but i keep the heater on low and that seems to help. My greatest fear atm is contams,Im really hoping it all goes well :dancer:

ill keep you posted

BF


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