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Offlinenewgrower82
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Registered: 04/03/11
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Liquid culture using wbs question
    #14397336 - 05/03/11 10:33 PM (12 years, 8 months ago)

I made my jars with the karo syrup and water and pressure cooked for the 20 minutes. When they cooled,in a glovebox opened a jar of fully colonized WBS and threw a small chunk in the karo water. It doesnt really look like much is happening and its been like 4 or five days.It does look a little cloudier though. Should I just toss them? Thanks
This site is awesome

these are pics of the 2 jars

[url=https://files.shroomery.org/files/11-18/448349693-lc_wbs_jar2.jpg][/url


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Offlinesteelmonkey
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Re: Liquid culture using wbs question [Re: newgrower82]
    #14397478 - 05/03/11 10:58 PM (12 years, 8 months ago)

What you did has a high contam rate and if there getting cloudy they probally are toast but you could wait and see what happens if its not threatening your grow area


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OfflineMOPE
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Registered: 10/07/08
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Re: Liquid culture using wbs question [Re: newgrower82]
    #14397530 - 05/03/11 11:06 PM (12 years, 8 months ago)

i cant really tell by the picture whether its cloudy because of the mycelium from the WBS, or if the sugar caramelized from over-PCing, or if its a contam. Like steelmonkey said, its pretty likely that the LC got contaminated

Do you know how to make a grain LC?  It would be a much better option than using colonized WBS to noc up a sugar LC


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Invisiblewildernessjunkie
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Registered: 06/13/10
Posts: 8,118
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Re: Liquid culture using wbs question [Re: steelmonkey]
    #14397543 - 05/03/11 11:08 PM (12 years, 8 months ago)

Quote:

steelmonkey said:
What you did has a high contam rate and if there getting cloudy they probally are toast but you could wait and see what happens if its not threatening your grow area


:whathesaid:


This would have been a better solution for you. Still could be if you still have unopened fully colonized WBS. Agar's Grain LC


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