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Offlinedball
Stranger
Registered: 03/22/11
Posts: 277
Last seen: 10 years, 5 months
After no growth for 6 days in one of my jars, I decided to open them
    #14396823 - 05/03/11 09:02 PM (12 years, 9 months ago)

and they reek of yeast!!! Any idea why???

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InvisibleFungal growth
Lootinint
Male User Gallery


Registered: 03/21/10
Posts: 3,641
Loc: under a rock in your yard
Re: After no growth for 6 days in one of my jars, I decided to open them [Re: dball]
    #14396828 - 05/03/11 09:04 PM (12 years, 9 months ago)

its because of the yeast growing in it.

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Offlinedball
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Registered: 03/22/11
Posts: 277
Last seen: 10 years, 5 months
Re: After no growth for 6 days in one of my jars, I decided to open them [Re: Fungal growth]
    #14396832 - 05/03/11 09:05 PM (12 years, 9 months ago)

Is that good or bad??? What does this mean?

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InvisibleBlindtheeye
All Seeing Nothing


Registered: 10/03/10
Posts: 2,573
Loc: TX Flag
Re: After no growth for 6 days in one of my jars, I decided to open them [Re: dball]
    #14396857 - 05/03/11 09:09 PM (12 years, 9 months ago)

That funky smell means your sub is contaminated.  Thats a bad thing

The only smell you want coming from your jars is mushroom


--------------------
A great truth cannot be communicated, it must be realized.

Edited by Blindtheeye (05/03/11 09:10 PM)

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Offlinedball
Stranger
Registered: 03/22/11
Posts: 277
Last seen: 10 years, 5 months
Re: After no growth for 6 days in one of my jars, I decided to open them [Re: Blindtheeye]
    #14396872 - 05/03/11 09:11 PM (12 years, 9 months ago)

This is the 2nd time in a row this has happened! What can be causing this?

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InvisibleBlindtheeye
All Seeing Nothing


Registered: 10/03/10
Posts: 2,573
Loc: TX Flag
Re: After no growth for 6 days in one of my jars, I decided to open them [Re: dball]
    #14396884 - 05/03/11 09:13 PM (12 years, 9 months ago)

Well the fact that you opened the jars makes me think your less than careful with your sterilization process


--------------------
A great truth cannot be communicated, it must be realized.

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Offlinedball
Stranger
Registered: 03/22/11
Posts: 277
Last seen: 10 years, 5 months
Re: After no growth for 6 days in one of my jars, I decided to open them [Re: Blindtheeye]
    #14396912 - 05/03/11 09:18 PM (12 years, 9 months ago)

I've done everything the same way the first time I inoculated them. This doesn't make any sense. What is the most likely cause of this?

1. I've used the same jars against, although I washed them in the dishwasher and boiled them for 90 minutes
2. My vermiculite bag had a broken lock, therefor it was opened in the closet.
3. My BRF was opened in my cabinet.

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InvisibleBlindtheeye
All Seeing Nothing


Registered: 10/03/10
Posts: 2,573
Loc: TX Flag
Re: After no growth for 6 days in one of my jars, I decided to open them [Re: dball]
    #14396933 - 05/03/11 09:22 PM (12 years, 9 months ago)

Your substrate material is going to be unclean untill you steralize it.  This is normal.  Do you ude a PC or do you steam? Do you use a glovebox?  Are all fans and stuff off when you Nocc your jars.  I mean the list goes on and on.  The most important prt of the process IMO is the sterilization.  Fuck that up and you got nothing.


--------------------
A great truth cannot be communicated, it must be realized.

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Offlinedball
Stranger
Registered: 03/22/11
Posts: 277
Last seen: 10 years, 5 months
Re: After no growth for 6 days in one of my jars, I decided to open them [Re: Blindtheeye]
    #14396934 - 05/03/11 09:22 PM (12 years, 9 months ago)

Quote:

Blindtheeye said:
Well the fact that you opened the jars makes me think your less than careful with your sterilization process




The substrate just did not look right. The inside of the jars just looked way too wet. I have 20 jars so I didn't mind opening just one of them.

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Offlinedball
Stranger
Registered: 03/22/11
Posts: 277
Last seen: 10 years, 5 months
Re: After no growth for 6 days in one of my jars, I decided to open them [Re: Blindtheeye]
    #14396949 - 05/03/11 09:25 PM (12 years, 9 months ago)

Quote:

Blindtheeye said:
Your substrate material is going to be unclean untill you steralize it.  This is normal.  Do you ude a PC or do you steam? Do you use a glovebox?  Are all fans and stuff off when you Nocc your jars.  I mean the list goes on and on.  The most important prt of the process IMO is the sterilization.  Fuck that up and you got nothing.




I steam for 90 minutes at a simmer. I use a glovebox. I really don't know how I could have messed this up.

I also cleaned my jars after using them with iso alcohol, but I washed them in the dishwasher after. I have no idea!

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