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Fischer
Teonanacatalyst


Registered: 06/03/10
Posts: 565
Loc: Downunder
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@Slapphappy (since quoting is messing my browser up)
I'd wonder whether a colonised/properly-cased monotub actually needs filtered air - there shouldn't be enough uncolonised nutrients for contaminant moulds to get a foothold (speaking with complete lack of experience).
And if you made a PF-cake and chucked some polyfill in the top instead of tyvek/tape/filter+lid , I'd put money on them turning funky colours with reasonable regularity. It might filter some particulate out, but fungal spores are too small (in my uneducated opinion) to be reliably stopped by any sort of loose fibre stuffing.
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Re: 2 cakes in 2 liter bottle [Re: Fischer]
#14397202 - 05/03/11 10:09 PM (12 years, 8 months ago) |
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Feel free to check out all the times that people use poly to filter their jar lids. Works great for grains, LC, pretty much everything!
As for established Mono's, no, you don't need the poly, nor do you need anything in the holes as the myc is fairly contam resistant on it's own.. Thats why you can have cakes in a sgfc without contaminating. When you first setup your monotub, you need either poly, micropore tape, or any other type of tape to keep contams out during colonization.
The OP is talking about a partially colonized substrate... Yes, he should have washed it off and then fruited... but he apparently didn't, so the substrate will keep colonizing as long as you keep contams out.
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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p_walter
Lurker



Registered: 04/25/11
Posts: 535
Loc: Irrelevant
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Re: 2 cakes in 2 liter bottle [Re: Fischer]
#14397578 - 05/03/11 11:15 PM (12 years, 8 months ago) |
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Quote:
Fischer said: @Slapphappy (since quoting is messing my browser up)
I'd wonder whether a colonised/properly-cased monotub actually needs filtered air - there shouldn't be enough uncolonised nutrients for contaminant moulds to get a foothold (speaking with complete lack of experience).
And if you made a PF-cake and chucked some polyfill in the top instead of tyvek/tape/filter+lid , I'd put money on them turning funky colours with reasonable regularity. It might filter some particulate out, but fungal spores are too small (in my uneducated opinion) to be reliably stopped by any sort of loose fibre stuffing.
On the first part, yes you are correct. As RR says, "there is nothing sterile about a fruiting chamber". What fruiting mycelia need is not sterility, but fresh air exchange, and lots of it. As for the second, unfortunately not so. To quote RR again, on sterilizing agar:
Quote:
Here's how to do it. Get a whiskey or wine bottle with a screw on lid that will fit in your PC. Drill a hole in the lid and stuff it with polyfill or cut a synthetic filter disk to fit inside the lid. Prepare your agar and pc in the normal way. The filter will prevent contaminants from entering the agar as it cools.
Synthetic filter disks are superior to polyfill, but polyfill will still do the job.
-------------------- Coaster said: so i got free drug coupons witch u floozies refer to as "money"....
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abusername
Stranger

Registered: 01/29/11
Posts: 12
Last seen: 12 years, 6 months
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Quote:
slapphappypill said: and if he's manually fanning, he's fine... it's in a GH, and the bottle is for a micro climate... I'd do the same thing is I was him for a cake. The only think i'd do different, is cut the bottom of the bottle off, set the cake in the GH like you would a SGFC on top of foil and verm, then put the top 80% of the bottle over it like a cover.
That way you have the holes and RH from the GH working for you. should work a lot better than fruiting without the bottle as I went through 6 gallons of water just to get my cakes through the first flush when I tried just cakes in my GH
Slaphappy did you mean to do this
VVV

i cut the top off and will cover it up with it?

and then i put the cakes on foil?
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Fischer
Teonanacatalyst


Registered: 06/03/10
Posts: 565
Loc: Downunder
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Re: 2 cakes in 2 liter bottle [Re: abusername] 1
#14397997 - 05/04/11 01:37 AM (12 years, 8 months ago) |
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I guess I can consider myself corrected on the polyfill. Thanks guys.
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Re: 2 cakes in 2 liter bottle [Re: abusername]
#14399584 - 05/04/11 11:51 AM (12 years, 8 months ago) |
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Quote:
abusername said:
Quote:
slapphappypill said: and if he's manually fanning, he's fine... it's in a GH, and the bottle is for a micro climate... I'd do the same thing is I was him for a cake. The only think i'd do different, is cut the bottom of the bottle off, set the cake in the GH like you would a SGFC on top of foil and verm, then put the top 80% of the bottle over it like a cover.
That way you have the holes and RH from the GH working for you. should work a lot better than fruiting without the bottle as I went through 6 gallons of water just to get my cakes through the first flush when I tried just cakes in my GH
Slaphappy did you mean to do this
VVV

i cut the top off and will cover it up with it?

and then i put the cakes on foil?
Put the foil dirrectly on the rack, cake on the foil, then flip the bottle upside down and place over the cakes. That way all you need to do to air it out is lift the bottle up and set aside for a few mins.
If your GH is dialed in, and your cool mist running, all you should need to do is leave the bottle off the cakes for a few minutes to mist and air out. After the cake has been fanned (aired) replace the bottle back over the top and you're golden brotha!
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Re: 2 cakes in 2 liter bottle [Re: Fischer] 1
#14399589 - 05/04/11 11:53 AM (12 years, 8 months ago) |
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Quote:
Fischer said: I guess I can consider myself corrected on the polyfill. Thanks guys.
No biggie man, we are all still learning, no one here knows everything about mycology, not even RR. Being able to step back and allow yourself to learn something says a lot about you bro, 5 mushies for ya!
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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