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dball
Stranger
Registered: 03/22/11
Posts: 277
Last seen: 10 years, 4 months
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if I wanted to create some LC's
#14394366 - 05/03/11 01:01 PM (12 years, 8 months ago) |
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Would these bad boys be the ones to do it from? They're not ESPECIALLY big, but they are the biggest out of my grow so far. The veil on the bottom has started to fall. Is it too late? Is it time to pick them?
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FractalXplora
Grainiack




Registered: 02/11/06
Posts: 2,494
Loc: UK
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Re: if I wanted to create some LC's [Re: dball]
#14394395 - 05/03/11 01:08 PM (12 years, 8 months ago) |
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creating a clean LC from a strand of shroom is a bit of a long shot but not impossible, your best bet would be to wipe the mushroom down in a glovebox with alcohol, take a sterilized syringe and shoot it through to take a needles worth of a strand and shoot it into your LC mix. (all done in a glovebox mind!)
I would make 2 - 3 LC.s as this is a hit ans miss method I found better take a few shots at it.
Theres a tek out there somewhere describing this in detail, maybe someone will chime in, cant recall what its called.
Nice cakes by the way, you wanna print those suckers.
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Edited by FractalXplora (05/03/11 01:10 PM)
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dball
Stranger
Registered: 03/22/11
Posts: 277
Last seen: 10 years, 4 months
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Re: if I wanted to create some LC's [Re: FractalXplora]
#14394404 - 05/03/11 01:12 PM (12 years, 8 months ago) |
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Quote:
FractalXplora said: creating a clean LC from a strand of shroom is a bit of a long shot but not impossible, your best bet would be to wipe the mushroom down in a glovebox with alchol, take a sterilized syringe and shoot it through to take a needles worth of a strand and shoot it into your LC mix. (all done in a glocebox mind!)
Theres a tek out there somewhere describing this in detail, maybe someone will chime in, cant recall what its called.
Nice cakes by the way, you wanna print those suckers. 
Mind my ignorance, but this is my first flush ever. My goal is to print it and then create a master LC then create smaller LCs for a bulk grow with bags since I don't want to spend $23 bucks everytime I want spores . Are they ready to pick yet? Do you have a recommended tek for printing? Preferably on a budget?
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theneatobandito



Registered: 02/26/10
Posts: 48
Loc: The Zeitgeist Movement
Last seen: 4 years, 1 month
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Re: if I wanted to create some LC's [Re: dball]
#14394423 - 05/03/11 01:16 PM (12 years, 8 months ago) |
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Do you have a glove box or flowhood? If so, printing is easily done on some aluminum foil, placed in a sanitized glove box or sterile airflow of a flowhood. They are ready to pick if the veil has already broken or fallen. Also, are you working with petri dishes & strain isolations, or planning to? You could easily take those to start cultures on petri dishes.
-------------------- “In oneself lies the whole world and if you know how to look and learn, the door is there and the key is in your hand. Nobody on earth can give you either the key or the door to open, except yourself.” - Jiddu Krishnamurti
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dball
Stranger
Registered: 03/22/11
Posts: 277
Last seen: 10 years, 4 months
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Quote:
theneatobandito said: Do you have a glove box or flowhood? If so, printing is easily done on some aluminum foil, placed in a sanitized glove box or sterile airflow of a flowhood. They are ready to pick if the veil has already broken or fallen. Also, are you working with petri dishes & strain isolations, or planning to? You could easily take those to start cultures on petri dishes.
Yeah I have a glove box I do my inoculation in. I assume you would use a petri dish for the spores to fall into? Is strain isolation just cloning the biggest shrooms? If so, then yes!
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wow
Lurking master



Registered: 07/27/06
Posts: 1,260
Loc: Cloud 9
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Re: if I wanted to create some LC's [Re: dball]
#14394446 - 05/03/11 01:23 PM (12 years, 8 months ago) |
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i have bad luck with lc's.. well.. not totally i usually make a couple and only use em one time then chuck em.. if you peel the stem in half long ways and bend it over so it looks like a "u" shape you can use a sterile syringe with a bit of sterile water or lc inside and get a core cutout from the fuzzy stuff inside the stem then shoot that into your lc.. its called a "lc clone" but i understand its not like an isolate, however it works great for me and better results than ms for sure!
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wow
Lurking master



Registered: 07/27/06
Posts: 1,260
Loc: Cloud 9
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Re: if I wanted to create some LC's [Re: wow]
#14394453 - 05/03/11 01:25 PM (12 years, 8 months ago) |
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an iso is on agar a non sectoring culture.. its mumbo man... lc clone will give you a nice consistent result as well IME...
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theneatobandito



Registered: 02/26/10
Posts: 48
Loc: The Zeitgeist Movement
Last seen: 4 years, 1 month
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Re: if I wanted to create some LC's [Re: wow]
#14394497 - 05/03/11 01:37 PM (12 years, 8 months ago) |
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Cloning the biggest mushroom is just more likely to produce ideal growth, in theory copying the growth you see in the mushroom you use. You can use a petri dish for the spores if you want.
Like wow said, isolation is actually taking a piece of growing mycelium on agar & choosing the growth that seems most promising. If you clone from a mushroom fruit body, there's no need to isolate anything; it's already a dykaryotic culture, so there really shouldn't be any need to pick desired growth, it should all be the same. The only case I could think of would be if you started to witness mutations, in which case the strain has gone through too many cell divisions, & is losing it's genetic line.
-------------------- “In oneself lies the whole world and if you know how to look and learn, the door is there and the key is in your hand. Nobody on earth can give you either the key or the door to open, except yourself.” - Jiddu Krishnamurti
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DynGBreeD
┌∩┐(◣_◢)┌∩┐


Registered: 01/15/11
Posts: 3,639
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Click Here if you are looking for the cloning method using LC
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