Home | Community | Message Board

Original Seeds Store
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Original Sensible Seeds High THC Strains   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleLuger0815
noob
Male

Registered: 12/21/10
Posts: 1,677
Loc: @ Home
.
    #14394204 - 05/03/11 12:20 PM (12 years, 9 months ago)

.

Edited by Luger0815 (07/03/11 05:44 PM)

Extras: Filter Print Post Top
Offlinerustycobwebs
Green mold cultivator

Registered: 12/17/05
Posts: 799
Last seen: 8 years, 10 months
Re: Boris said.....dont shake a jar after inoc. [Re: Luger0815]
    #14394214 - 05/03/11 12:22 PM (12 years, 9 months ago)

Good question. In an LC they should be able to find each other anyway as they're floating in liquid.

You'll have to stir it sometime....

Give it a couple days to be safe. I usually just drop a grain in there or a pin, but when I used spores once I waited a week then did daily stirrings and everything turned out fine.


--------------------
and let it be so Praise Psilocybe

Extras: Filter Print Post Top
InvisibleLuger0815
noob
Male

Registered: 12/21/10
Posts: 1,677
Loc: @ Home
Re: Boris said.....dont shake a jar after innoc. [Re: rustycobwebs]
    #14394237 - 05/03/11 12:29 PM (12 years, 9 months ago)

.

Edited by Luger0815 (07/03/11 05:44 PM)

Extras: Filter Print Post Top
Offlineegodeathflux
Guttersnipe
Male User Gallery


Registered: 02/02/10
Posts: 3,854
Loc: The Stygian Pits Flag
Last seen: 1 year, 3 months
Re: Boris said.....dont shake a jar after inoc. [Re: Luger0815]
    #14394251 - 05/03/11 12:31 PM (12 years, 9 months ago)

Quote:

Does the advice "not to shake a jar after inoculation" also maters for LC's?





It sounds like you're asking if a jar noc'd with LC needs or is harmed by shaking, not sure the previous post addressed that. I don't shake mine until they are showing healthy growth if noc'd with LC.

I did some LC jars 2 days ago, if you want I'll shake 2 out of 5 now and let you know the growth/results of the 2 side by side. I have visible myc in all 5 jars already and I'd be interested to know myself. I stick with the 30%-%40 shaking rule, but only cos I don't know any better. :popcorn:


--------------------

"Atrophic interludes weave through my life far too often, for me to fight the biggest enemies"




"Standing on the corner of 5th and Vermouth"


Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: Boris said.....dont shake a jar after inoc. [Re: egodeathflux]
    #14394256 - 05/03/11 12:33 PM (12 years, 9 months ago)

Shake after shooting LC, it's live dikaryotic mycelium.
Shake well at inoculation and you may not need to shake again until spawning.


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
OfflineDave Bowman
Albert Hoffmans Apprentice
Male User Gallery


Folding@home Statistics
Registered: 08/30/07
Posts: 2,104
Loc: Your Imagination
Last seen: 4 months, 25 days
Re: Boris said.....dont shake a jar after innoc. [Re: Luger0815]
    #14394260 - 05/03/11 12:33 PM (12 years, 9 months ago)

Quote:

Luger0815 said:
No, you get me wrong.
I meant after innoc. a jar of rye with an LC.
Is it important, not to shake the rye jar after innoc. until it gets 30% colonized, to get more Rizomorphic growth?
Or does it only maters if i innoc. with spores?!




When I use LC on grain jars... I wait to shake until around 40% or so... then once again later on if there is uneven growth.

I never shake DIRECTLY after inoculation.

Extras: Filter Print Post Top
InvisibleSillicybin
Registered: 02/14/05
Posts: 2,134
Loc: Flag
Re: Boris said.....dont shake a jar after inoc. [Re: Luger0815]
    #14394263 - 05/03/11 12:34 PM (12 years, 9 months ago)

Quote:

Luger0815 said:
Does the advice "not to shake a jar after inoculation" also maters for LC's?




NO!!!

I break up the grain with a couple hits and then roll the jars so that the LC coats everywhere I can get it.  LC is already dikaryotic, meaning the hyphae have already found a mate.  They will reproduce just fine without finding other hyphae.

Extras: Filter Print Post Top
InvisibleLuger0815
noob
Male

Registered: 12/21/10
Posts: 1,677
Loc: @ Home
Re: Boris said.....dont shake a jar after innoc. [Re: Doc_T]
    #14394276 - 05/03/11 12:37 PM (12 years, 9 months ago)

.
.

Edited by Luger0815 (07/03/11 05:44 PM)

Extras: Filter Print Post Top
Offlineegodeathflux
Guttersnipe
Male User Gallery


Registered: 02/02/10
Posts: 3,854
Loc: The Stygian Pits Flag
Last seen: 1 year, 3 months
Re: Boris said.....dont shake a jar after inoc. [Re: Sillicybin]
    #14394286 - 05/03/11 12:41 PM (12 years, 9 months ago)

I may go and shake all my jars... Never really given it much thought, LCs colonise so fast anyway.

My LCs usually come out the fridge, so I always allowed them a couple of days to re-animate/come out of stasis after noc'ing. I'm pretty generous with my LC amounts too, they are usually 70-80% done in 7 days (1000ml jars, rye).


--------------------

"Atrophic interludes weave through my life far too often, for me to fight the biggest enemies"




"Standing on the corner of 5th and Vermouth"


Extras: Filter Print Post Top
InvisibleLuger0815
noob
Male

Registered: 12/21/10
Posts: 1,677
Loc: @ Home
Re: Boris said.....dont shake a jar after innoc. [Re: egodeathflux]
    #14394319 - 05/03/11 12:47 PM (12 years, 9 months ago)

I also use LC's a lot but this time it just dont perform very well.
It shows almost only cottony myc. instead of rhizo's, and seem to stall.
It is 100% covering the berrys but it seems that the growth just stops somehow.:shrug:
Maybe i did something wrong this time, never mind.
As Doc always state: LC's suck!
Not because of the chance to get contams, more because it is always sugar based and multi spore/strain.
Time to move to agar.

Edited by Luger0815 (05/03/11 01:50 PM)

Extras: Filter Print Post Top
InvisibleSillicybin
Registered: 02/14/05
Posts: 2,134
Loc: Flag
Re: Boris said.....dont shake a jar after innoc. [Re: Luger0815]
    #14394333 - 05/03/11 12:52 PM (12 years, 9 months ago)

Quote:

Luger0815 said:
I also use LC's a lot but this time it just dont perform very well.



How old is your LC?  Have you been storing it in the refrigerator?

If you're not getting vigorous growth, you may have to go back to multispore and make another LC or GLC.

Extras: Filter Print Post Top
Invisibleslapphappypill
Enthusiast!
 User Gallery


Folding@home Statistics
Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
Re: Boris said.....dont shake a jar after innoc. [Re: Luger0815]
    #14394353 - 05/03/11 12:56 PM (12 years, 9 months ago)

Quote:

Luger0815 said:
Quote:

Doc_T said:
Shake after shooting LC, it's live dikaryotic mycelium.
Shake well at inoculation and you may not need to shake again until spawning.




This was exactly, what i wanted to know.
Thank you Doc.

And thanks to all the others that replied that fast.:thumbup:




I'm at work and can't read everything, but I ALWAYS shake my jars RIGHT after I knock them up with a good LC. I shake for at least 2 mins per jar, up to 4 or 5 mins. After I shake mine, I never have to shake again, and if you do it well enough, your LC can colonize a full qt in a week, and ready for spawn in 1.5 weeks. Works GREAT!

Now for me personally, I have issues with my jars if I shake at 30%-40%, they stall or something. If your using spores, let them germinate, then shake at 20-30%. make sure to shake VERY well!


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~

Extras: Filter Print Post Top
Offlineegodeathflux
Guttersnipe
Male User Gallery


Registered: 02/02/10
Posts: 3,854
Loc: The Stygian Pits Flag
Last seen: 1 year, 3 months
Re: Boris said.....dont shake a jar after innoc. [Re: Luger0815]
    #14394361 - 05/03/11 12:59 PM (12 years, 9 months ago)

Quote:

Luger0815 said:
As Doc always state: LC's suck!
Not because of the chance to get contams, more because it is always sugar based and multi spore/strain.
Time to move to agar.




You can of course make an LC from an agar wedge.. the two are not mutually exclusive. LCs are a lot less hassle than spore syringes etc. I have a 100% clean, no contam record with LCs. :shrug:


--------------------

"Atrophic interludes weave through my life far too often, for me to fight the biggest enemies"




"Standing on the corner of 5th and Vermouth"


Extras: Filter Print Post Top
InvisibleLuger0815
noob
Male

Registered: 12/21/10
Posts: 1,677
Loc: @ Home
Re: Boris said.....dont shake a jar after innoc. [Re: Sillicybin]
    #14394364 - 05/03/11 12:59 PM (12 years, 9 months ago)

.

Edited by Luger0815 (07/03/11 05:44 PM)

Extras: Filter Print Post Top
InvisibleLuger0815
noob
Male

Registered: 12/21/10
Posts: 1,677
Loc: @ Home
Re: Boris said.....dont shake a jar after innoc. [Re: egodeathflux]
    #14394375 - 05/03/11 01:02 PM (12 years, 9 months ago)

Quote:

egodeathflux said:
Quote:

Luger0815 said:
As Doc always state: LC's suck!
Not because of the chance to get contams, more because it is always sugar based and multi spore/strain.
Time to move to agar.




You can of course make an LC from an agar wedge.. the two are not mutually exclusive. LCs are a lot less hassle than spore syringes etc. I have a 100% clean, no contam record with LCs. :shrug:




I also never had a contaminated LC.
But i think that isolating or cloning on agar will get some better results.:shrug:

Extras: Filter Print Post Top
Offlineegodeathflux
Guttersnipe
Male User Gallery


Registered: 02/02/10
Posts: 3,854
Loc: The Stygian Pits Flag
Last seen: 1 year, 3 months
Re: Boris said.....dont shake a jar after innoc. [Re: Luger0815]
    #14394406 - 05/03/11 01:12 PM (12 years, 9 months ago)

Agar will undoubtedly produce better results, so why not stick it in an LC and have more of it? :mushroom2:


--------------------

"Atrophic interludes weave through my life far too often, for me to fight the biggest enemies"




"Standing on the corner of 5th and Vermouth"


Extras: Filter Print Post Top
InvisibleLuger0815
noob
Male

Registered: 12/21/10
Posts: 1,677
Loc: @ Home
Re: Boris said.....dont shake a jar after innoc. [Re: egodeathflux]
    #14394424 - 05/03/11 01:16 PM (12 years, 9 months ago)

.

Edited by Luger0815 (07/03/11 05:45 PM)

Extras: Filter Print Post Top
Invisibleslapphappypill
Enthusiast!
 User Gallery


Folding@home Statistics
Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
Re: Boris said.....dont shake a jar after innoc. [Re: egodeathflux]
    #14394429 - 05/03/11 01:19 PM (12 years, 9 months ago)

Quote:

egodeathflux said:
Agar will undoubtedly produce better results, so why not stick it in an LC and have more of it? :mushroom2:




Thats how I make my LCs as well! I have even been successful with open air agar to LC. I wouldn't recomend it, but it still works!


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~

Extras: Filter Print Post Top
InvisibleLuger0815
noob
Male

Registered: 12/21/10
Posts: 1,677
Loc: @ Home
Re: Boris said.....dont shake a jar after innoc. [Re: slapphappypill]
    #14394454 - 05/03/11 01:25 PM (12 years, 9 months ago)

.

Edited by Luger0815 (07/03/11 05:45 PM)

Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: Boris said.....dont shake a jar after innoc. [Re: egodeathflux]
    #14398000 - 05/04/11 01:39 AM (12 years, 9 months ago)

Quote:

egodeathflux said:
Agar will undoubtedly produce better results, so why not stick it in an LC and have more of it? :mushroom2:




Better: put agar in grain jar, make grain LC.


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Original Sensible Seeds High THC Strains   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale


Similar ThreadsPosterViewsRepliesLast post
* can over shaking jars (whole grain) be a problem? trippy 5,009 18 10/03/02 08:33 PM
by trippy
* Re: shake jars after inoculation?? n8 2,715 8 02/16/00 01:09 PM
by Anonymous
* Shake jars imediatly after steralization or later? d fuNk22 2,633 4 06/12/02 03:42 PM
by luvdemshrooms
* Shaking jars of rye Auditory Misperception 1,589 7 03/03/13 04:35 PM
by Jabbawaya
* Shaking Jars? ble0t 1,678 2 09/19/01 06:00 PM
by oscill8
* Shaking jars may slow growth Swami 3,411 5 03/11/02 08:23 AM
by Swami
* Shake Jar after innoculation? WhosYourPal 1,243 1 10/23/01 09:10 AM
by bluhoney
* Re: quart jars?? Humidity 1,177 4 04/17/00 10:42 PM
by Humidity

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,546 topic views. 17 members, 117 guests and 31 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.005 seconds on 14 queries.