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Luger0815
noob


Registered: 12/21/10
Posts: 1,677
Loc: @ Home
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#14394204 - 05/03/11 12:20 PM (12 years, 8 months ago) |
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Edited by Luger0815 (07/03/11 05:44 PM)
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rustycobwebs
Green mold cultivator

Registered: 12/17/05
Posts: 799
Last seen: 8 years, 9 months
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Re: Boris said.....dont shake a jar after inoc. [Re: Luger0815]
#14394214 - 05/03/11 12:22 PM (12 years, 8 months ago) |
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Good question. In an LC they should be able to find each other anyway as they're floating in liquid.
You'll have to stir it sometime....
Give it a couple days to be safe. I usually just drop a grain in there or a pin, but when I used spores once I waited a week then did daily stirrings and everything turned out fine.
-------------------- and let it be so Praise Psilocybe
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Luger0815
noob


Registered: 12/21/10
Posts: 1,677
Loc: @ Home
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Re: Boris said.....dont shake a jar after innoc. [Re: rustycobwebs]
#14394237 - 05/03/11 12:29 PM (12 years, 8 months ago) |
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Edited by Luger0815 (07/03/11 05:44 PM)
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egodeathflux
Guttersnipe



Registered: 02/02/10
Posts: 3,854
Loc: The Stygian Pits
Last seen: 1 year, 2 months
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Re: Boris said.....dont shake a jar after inoc. [Re: Luger0815]
#14394251 - 05/03/11 12:31 PM (12 years, 8 months ago) |
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Quote:
Does the advice "not to shake a jar after inoculation" also maters for LC's?
It sounds like you're asking if a jar noc'd with LC needs or is harmed by shaking, not sure the previous post addressed that. I don't shake mine until they are showing healthy growth if noc'd with LC.
I did some LC jars 2 days ago, if you want I'll shake 2 out of 5 now and let you know the growth/results of the 2 side by side. I have visible myc in all 5 jars already and I'd be interested to know myself. I stick with the 30%-%40 shaking rule, but only cos I don't know any better.
-------------------- "Atrophic interludes weave through my life far too often, for me to fight the biggest enemies" "Standing on the corner of 5th and Vermouth"
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Boris said.....dont shake a jar after inoc. [Re: egodeathflux]
#14394256 - 05/03/11 12:33 PM (12 years, 8 months ago) |
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Shake after shooting LC, it's live dikaryotic mycelium. Shake well at inoculation and you may not need to shake again until spawning.
-------------------- You make it all possible. Doesn't it feel good?
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Dave Bowman
Albert Hoffmans Apprentice




Registered: 08/30/07
Posts: 2,104
Loc: Your Imagination
Last seen: 3 months, 24 days
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Re: Boris said.....dont shake a jar after innoc. [Re: Luger0815]
#14394260 - 05/03/11 12:33 PM (12 years, 8 months ago) |
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Quote:
Luger0815 said: No, you get me wrong. I meant after innoc. a jar of rye with an LC. Is it important, not to shake the rye jar after innoc. until it gets 30% colonized, to get more Rizomorphic growth? Or does it only maters if i innoc. with spores?!
When I use LC on grain jars... I wait to shake until around 40% or so... then once again later on if there is uneven growth.
I never shake DIRECTLY after inoculation.
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Sillicybin
Registered: 02/14/05
Posts: 2,134
Loc:
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Re: Boris said.....dont shake a jar after inoc. [Re: Luger0815]
#14394263 - 05/03/11 12:34 PM (12 years, 8 months ago) |
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Quote:
Luger0815 said: Does the advice "not to shake a jar after inoculation" also maters for LC's?
NO!!!
I break up the grain with a couple hits and then roll the jars so that the LC coats everywhere I can get it. LC is already dikaryotic, meaning the hyphae have already found a mate. They will reproduce just fine without finding other hyphae.
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Luger0815
noob


Registered: 12/21/10
Posts: 1,677
Loc: @ Home
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Re: Boris said.....dont shake a jar after innoc. [Re: Doc_T]
#14394276 - 05/03/11 12:37 PM (12 years, 8 months ago) |
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Edited by Luger0815 (07/03/11 05:44 PM)
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egodeathflux
Guttersnipe



Registered: 02/02/10
Posts: 3,854
Loc: The Stygian Pits
Last seen: 1 year, 2 months
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Re: Boris said.....dont shake a jar after inoc. [Re: Sillicybin]
#14394286 - 05/03/11 12:41 PM (12 years, 8 months ago) |
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I may go and shake all my jars... Never really given it much thought, LCs colonise so fast anyway.
My LCs usually come out the fridge, so I always allowed them a couple of days to re-animate/come out of stasis after noc'ing. I'm pretty generous with my LC amounts too, they are usually 70-80% done in 7 days (1000ml jars, rye).
-------------------- "Atrophic interludes weave through my life far too often, for me to fight the biggest enemies" "Standing on the corner of 5th and Vermouth"
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Luger0815
noob


Registered: 12/21/10
Posts: 1,677
Loc: @ Home
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Re: Boris said.....dont shake a jar after innoc. [Re: egodeathflux]
#14394319 - 05/03/11 12:47 PM (12 years, 8 months ago) |
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I also use LC's a lot but this time it just dont perform very well. It shows almost only cottony myc. instead of rhizo's, and seem to stall. It is 100% covering the berrys but it seems that the growth just stops somehow. Maybe i did something wrong this time, never mind. As Doc always state: LC's suck! Not because of the chance to get contams, more because it is always sugar based and multi spore/strain. Time to move to agar.
Edited by Luger0815 (05/03/11 01:50 PM)
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Sillicybin
Registered: 02/14/05
Posts: 2,134
Loc:
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Re: Boris said.....dont shake a jar after innoc. [Re: Luger0815]
#14394333 - 05/03/11 12:52 PM (12 years, 8 months ago) |
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Quote:
Luger0815 said: I also use LC's a lot but this time it just dont perform very well.
How old is your LC? Have you been storing it in the refrigerator?
If you're not getting vigorous growth, you may have to go back to multispore and make another LC or GLC.
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Re: Boris said.....dont shake a jar after innoc. [Re: Luger0815]
#14394353 - 05/03/11 12:56 PM (12 years, 8 months ago) |
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Quote:
Luger0815 said:
Quote:
Doc_T said: Shake after shooting LC, it's live dikaryotic mycelium. Shake well at inoculation and you may not need to shake again until spawning.
This was exactly, what i wanted to know. Thank you Doc.
And thanks to all the others that replied that fast.
I'm at work and can't read everything, but I ALWAYS shake my jars RIGHT after I knock them up with a good LC. I shake for at least 2 mins per jar, up to 4 or 5 mins. After I shake mine, I never have to shake again, and if you do it well enough, your LC can colonize a full qt in a week, and ready for spawn in 1.5 weeks. Works GREAT!
Now for me personally, I have issues with my jars if I shake at 30%-40%, they stall or something. If your using spores, let them germinate, then shake at 20-30%. make sure to shake VERY well!
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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egodeathflux
Guttersnipe



Registered: 02/02/10
Posts: 3,854
Loc: The Stygian Pits
Last seen: 1 year, 2 months
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Re: Boris said.....dont shake a jar after innoc. [Re: Luger0815]
#14394361 - 05/03/11 12:59 PM (12 years, 8 months ago) |
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Quote:
Luger0815 said: As Doc always state: LC's suck! Not because of the chance to get contams, more because it is always sugar based and multi spore/strain. Time to move to agar.
You can of course make an LC from an agar wedge.. the two are not mutually exclusive. LCs are a lot less hassle than spore syringes etc. I have a 100% clean, no contam record with LCs.
-------------------- "Atrophic interludes weave through my life far too often, for me to fight the biggest enemies" "Standing on the corner of 5th and Vermouth"
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Luger0815
noob


Registered: 12/21/10
Posts: 1,677
Loc: @ Home
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Re: Boris said.....dont shake a jar after innoc. [Re: Sillicybin]
#14394364 - 05/03/11 12:59 PM (12 years, 8 months ago) |
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Edited by Luger0815 (07/03/11 05:44 PM)
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Luger0815
noob


Registered: 12/21/10
Posts: 1,677
Loc: @ Home
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Re: Boris said.....dont shake a jar after innoc. [Re: egodeathflux]
#14394375 - 05/03/11 01:02 PM (12 years, 8 months ago) |
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Quote:
egodeathflux said:
Quote:
Luger0815 said: As Doc always state: LC's suck! Not because of the chance to get contams, more because it is always sugar based and multi spore/strain. Time to move to agar.
You can of course make an LC from an agar wedge.. the two are not mutually exclusive. LCs are a lot less hassle than spore syringes etc. I have a 100% clean, no contam record with LCs. 
I also never had a contaminated LC. But i think that isolating or cloning on agar will get some better results.
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egodeathflux
Guttersnipe



Registered: 02/02/10
Posts: 3,854
Loc: The Stygian Pits
Last seen: 1 year, 2 months
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Re: Boris said.....dont shake a jar after innoc. [Re: Luger0815]
#14394406 - 05/03/11 01:12 PM (12 years, 8 months ago) |
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Agar will undoubtedly produce better results, so why not stick it in an LC and have more of it?
-------------------- "Atrophic interludes weave through my life far too often, for me to fight the biggest enemies" "Standing on the corner of 5th and Vermouth"
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Luger0815
noob


Registered: 12/21/10
Posts: 1,677
Loc: @ Home
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Re: Boris said.....dont shake a jar after innoc. [Re: egodeathflux]
#14394424 - 05/03/11 01:16 PM (12 years, 8 months ago) |
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Edited by Luger0815 (07/03/11 05:45 PM)
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Re: Boris said.....dont shake a jar after innoc. [Re: egodeathflux]
#14394429 - 05/03/11 01:19 PM (12 years, 8 months ago) |
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Quote:
egodeathflux said: Agar will undoubtedly produce better results, so why not stick it in an LC and have more of it? 
Thats how I make my LCs as well! I have even been successful with open air agar to LC. I wouldn't recomend it, but it still works!
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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Luger0815
noob


Registered: 12/21/10
Posts: 1,677
Loc: @ Home
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Re: Boris said.....dont shake a jar after innoc. [Re: slapphappypill]
#14394454 - 05/03/11 01:25 PM (12 years, 8 months ago) |
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Edited by Luger0815 (07/03/11 05:45 PM)
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Boris said.....dont shake a jar after innoc. [Re: egodeathflux]
#14398000 - 05/04/11 01:39 AM (12 years, 8 months ago) |
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Quote:
egodeathflux said: Agar will undoubtedly produce better results, so why not stick it in an LC and have more of it? 
Better: put agar in grain jar, make grain LC.
-------------------- You make it all possible. Doesn't it feel good?
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13shrooms
Lightning Shaman



Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: Boris said.....dont shake a jar after innoc. [Re: Doc_T]
#14398026 - 05/04/11 01:51 AM (12 years, 8 months ago) |
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Quote:
Doc_T said:
Quote:
egodeathflux said: Agar will undoubtedly produce better results, so why not stick it in an LC and have more of it? 
Better: put agar in grain jar, make grain LC.
thats my preferred method now but once I get my flowhood built > isolate on agar > to a grain jar > G2G to jars/bags > spawn to bulk 
And yes you shake after inoculating w/LCs to distribute the live myc all over your grains, spores are the only thing you dont wana shake-up/seperate after inoculation. 
--------------------
A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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Luger0815
noob


Registered: 12/21/10
Posts: 1,677
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Re: Boris said.....dont shake a jar after innoc. [Re: 13shrooms]
#14398125 - 05/04/11 02:26 AM (12 years, 8 months ago) |
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Thanks Doc and 13. +5 for that. That sounds like the best method's for me.
Edited by Luger0815 (05/04/11 02:33 AM)
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