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Offlinejremines
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shook brf jars
    #14390767 - 05/02/11 07:41 PM (12 years, 8 months ago)

i was doin some more reading on the subject of shaking..I shook my BRF jars right after inoculation..bad idea? or doesnt matter? I read posts with both opinions..any reasons for not shaking the jars as opposed to shaking?


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Re: shook brf jars [Re: jremines]
    #14390787 - 05/02/11 07:45 PM (12 years, 8 months ago)

I would say bad idea considering the top layer of verm is your filter, so while shaking it you loose your filter leading to possible conatam. But I still am in the learning process so don't take my word for it.


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Re: shook brf jars [Re: muffinTop]
    #14390851 - 05/02/11 07:53 PM (12 years, 8 months ago)

i guess it depends on if you used a filter or if you're depending on the verm to stop contams from getting in. if you used a filter i'd say you're fine, but if not i'd go with muffinTop on this one


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Re: shook brf jars [Re: jremines]
    #14390885 - 05/02/11 07:58 PM (12 years, 8 months ago)

Quote:

jremines said:
..I shook my BRF jars right after inoculation..bad idea?




haha..

but no seriously..i'm guessing your jars were "sterile" when you nocd them up, including the verm layer (to a certain extent anyway). I wouldn't THINK shaking them up that early would be "bad". I'm no expert, but it just seems like if you shook the jars up after sitting and collecting dust like 2 weeks AFTER you noccd them up.. well i guess the verm would be dirty or at least have some contamination, unlike when you first inoculated.


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Re: shook brf jars [Re: LayinUp]
    #14391235 - 05/02/11 08:58 PM (12 years, 8 months ago)

Possibly the worst thing you could do with BRF jars. Shaking is for grain jars, my friend, not BRF.

When you originally added substrate to the jars, you should have loaded it really lightly, and VERY GENTLY tamped it level around the edges before adding the dry verm. You do it that way to avoid compacting the substrate, which can lead to incomplete colonization. By shaking it, not only have you risked contamination by particles that fell in to the dry verm layer, but I imagine you compacted the ever-loving crap out of the BRF mixture in the jar.

I can't imagine anyone saying this would ever be a good idea. Sorry for your fail, better luck next time. :biggrin:


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Offlinejremines
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Re: shook brf jars [Re: LayinUp]
    #14391270 - 05/02/11 09:03 PM (12 years, 8 months ago)

there filtered for sure, and were sterile before i knocked them up..i was just wondering if it was bad for the spores..i got my GE on the right track finally, now im going down the line of posibilities..its just takin a little longer than i expected to see growth so im goin over everything i can think of..


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Re: shook brf jars [Re: jremines]
    #14391327 - 05/02/11 09:11 PM (12 years, 8 months ago)

how do you even shake a brf jar


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Re: shook brf jars [Re: ic3.qu33n]
    #14391366 - 05/02/11 09:15 PM (12 years, 8 months ago)

It is bad for the spores.  Only because each spore needs to find a compatible "mate".  When you shake it makes the spores harder to find each other......Never shake BRF cakes though that is very bad....Especially if the holes were open on the top or any space for air to creep in...


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Re: shook brf jars [Re: i GrOw StUFF]
    #14391461 - 05/02/11 09:32 PM (12 years, 8 months ago)

DON'T SHAKE BRF  :thumbdown:


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Offlinejremines
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Re: shook brf jars [Re: mabachko]
    #14393407 - 05/03/11 08:40 AM (12 years, 8 months ago)

i just checked the jars this morning...the layer of dry verm on the top is still 100% intact, the holes at the top are covered with micro pore..and the sub in my jars is still loose with tons of crevaces and holes so its still lightly packed..just worried cause its taking so long to show signs of colonozation..no funny smells or growths tho..its been about 2 weeks now with no signs..


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Re: shook brf jars [Re: ic3.qu33n]
    #14393415 - 05/03/11 08:42 AM (12 years, 8 months ago)

Quote:

ic3.qu33n said:
how do you even shake a brf jar




That was my question. Exactly. :shrug:


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Re: shook brf jars [Re: Bstsneverr]
    #14393444 - 05/03/11 08:51 AM (12 years, 8 months ago)

with your hands...like you would shake anything else..


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Re: shook brf jars [Re: jremines]
    #14393455 - 05/03/11 08:54 AM (12 years, 8 months ago)

Lmao... The point is don't ever ever shake BRF jars... A little research would have yielded the same information.


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*** Psalm 9:9-10 ***

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Re: shook brf jars [Re: Bstsneverr]
    #14393470 - 05/03/11 08:59 AM (12 years, 8 months ago)

May i add that there, well, shouldn't be room in a BRF jar to begin with to shake.


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*** Psalm 9:9-10 ***

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Offlinejremines
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Re: shook brf jars [Re: Bstsneverr]
    #14393532 - 05/03/11 09:18 AM (12 years, 8 months ago)

first timer here..and i did a "little" research thats why i had to ask this question..because most of the posts are people arguing about it..no straight forward answer..i didnt personally make the jars..i got them from a guy..they're one pint, with the layer of dry verm at the top..which is still there, and i have the holes covered..im not really worried about contams at this point..more worried about whether the jars were junk to begin with..or if i mest it up by shaking..


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Re: shook brf jars [Re: Bstsneverr]
    #14393549 - 05/03/11 09:22 AM (12 years, 8 months ago)

Quote:

Bstsneverr said:
Quote:

ic3.qu33n said:
how do you even shake a brf jar




That was my question. Exactly. :shrug:




Smack it against your other palm.
If you've used grain-style lids instead of a dry verm layer, it makes sense.


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Re: shook brf jars [Re: jremines]
    #14393562 - 05/03/11 09:24 AM (12 years, 8 months ago)

unless you shook the dog-shit out of them, the pf sub didn't move, so it didn't distribute anything. Contrary to what peaple have been saying, a little side to side jiggle, right after inoculation, and before you open the g/b is a good thing...to fill the holes in your verm. layer left by the syringe. Leave it on a flat surface and just move it back and forth quickly with wrist motion. About 2 shakes will do it


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Offlinejremines
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Re: shook brf jars [Re: SomeGuy]
    #14393585 - 05/03/11 09:30 AM (12 years, 8 months ago)

finally some help...thanks fellas..i see you two on here the most probably so ill take your word for it..and ideas on why they're takin so long to show signs?


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Re: shook brf jars [Re: jremines]
    #14393587 - 05/03/11 09:31 AM (12 years, 8 months ago)

How long?


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Re: shook brf jars [Re: Doc_T]
    #14393612 - 05/03/11 09:38 AM (12 years, 8 months ago)

its hitting on 2 weeks now, no signs of anything good or bad


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Re: shook brf jars [Re: jremines]
    #14393623 - 05/03/11 09:42 AM (12 years, 8 months ago)

Good job waiting two weeks. :thumbup:

At this point you should probably have seen something.
Give it another week before you toss them, but start on Plan B now is my advice.
Agar is cheaper and easier than it seems... get a little pressure cooker. :wink:


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Re: shook brf jars [Re: Doc_T]
    #14393634 - 05/03/11 09:46 AM (12 years, 8 months ago)

do i have to toss it completely, or can i just re-cook everything and shoot em up again? ive been doing alot of reading about agar on here..it seems like it has a wider range of uses as well, and alot more easier to work with when it concerns contams..im really unsure about it tho


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Re: shook brf jars [Re: jremines]
    #14393641 - 05/03/11 09:49 AM (12 years, 8 months ago)

Why would you recook them? Why would you risk you next grow on jars that aren't fresh?


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Re: shook brf jars [Re: Doc_T]
    #14393648 - 05/03/11 09:53 AM (12 years, 8 months ago)

these are the things i wonder..the jars are ok to keep tho? just toss the contents?..what about the lids? they had a bit of rust from the PC time im guessings? i bought from what i was told was a reputable dealer..


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Re: shook brf jars [Re: jremines]
    #14393683 - 05/03/11 10:05 AM (12 years, 8 months ago)

Oh the jars and lids are fine. Wash with warm soapy water, rinse, air dry.


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Re: shook brf jars [Re: Doc_T]
    #14393701 - 05/03/11 10:10 AM (12 years, 8 months ago)

nice, thanks..one more question if its not to much trouble for ya..i have 3 ccs left over in the fridge, no prints obviously..LC thde last 3 ccs? or do a couple jars and transfer myc?


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Re: shook brf jars [Re: jremines]
    #14393709 - 05/03/11 10:14 AM (12 years, 8 months ago)

Save the last few drops to use for LC. And a few drops ahead of that for a few agar plates.
Stretch the rest in regular jars, using just a tiny bit in each hole, enough to trickle down the glass.
Is my advice.


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Re: shook brf jars [Re: Doc_T]
    #14393727 - 05/03/11 10:21 AM (12 years, 8 months ago)

ok, im gonna wait out another week before i do anything..ill check the jars once daily for any signs of life..if nothing by this time next week ill hock the project and start over..now i just need to resupply!!


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Re: shook brf jars [Re: SomeGuy]
    #14393758 - 05/03/11 10:37 AM (12 years, 8 months ago)

Quote:

paducahovoids said:
just move it back and forth quickly with wrist motion. About 2 shakes will do it



i did this with 2 of my jars and i had 6-8 spots of germination rather than the 4 big spots that start to form. kept it flat and gave 2-3 quick wrist spins.


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Re: shook brf jars [Re: lostinbq]
    #14393812 - 05/03/11 10:53 AM (12 years, 8 months ago)

that's not the reason why. You just had several spores germinate. If the pf sub moves, big mistake. You can wait another week, but you are nearing the end of hope that they are gonna take off. If they didn't take off on the pf cake, they most likely aren't going to do any better on agar or in an lc. Of course, unless the jars you got were just old and dried out


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Re: shook brf jars [Re: SomeGuy]
    #14393916 - 05/03/11 11:17 AM (12 years, 8 months ago)

yea i kno i had several spores germinate. but like i said they were all across the jar, not just at the inoculation points. some were at the inoc points some were scattered around the jar. i only did the spin cous of like you said, to redistribute the contam barrier. but also gave me surprising results.


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Re: shook brf jars [Re: lostinbq]
    #14394373 - 05/03/11 01:02 PM (12 years, 8 months ago)

the jars i got were purchased from a store, ive noticed an amount of condinsation inside the jars..when i got my spore syringes they were clear..i did the shake like hell stuff, but didnt really even show and dispersment..it just looked like water in a syringe..ill wait another week, and see..is it possible that its colonizing the inside of the cake first or is that just a stupid noob theory?


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Re: shook brf jars [Re: jremines]
    #14395035 - 05/03/11 03:47 PM (12 years, 8 months ago)

should have seen something. i made 10 jars with a single syringe and all showed something by day 4, well a inoculation spot did not take and by the time the myc grew to it i had removed the jar and isolated cos that spot started to grow idk maby a week after inoculation, then i liked at it a couple days later and realized it was from an inoculation spot and it was fine.might have been high at the first time i saw it. i was talking to a guy recently and after he waited 2 weeks we discovered he had a bunk syringe. clear you say it was, bunk your syringe i believe. you should have seen some lil  black in the syringe.


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Re: shook brf jars [Re: jremines]
    #14395143 - 05/03/11 04:12 PM (12 years, 8 months ago)

Quote:

jremines said:
the jars i got were purchased from a store, ive noticed an amount of condinsation inside the jars..when i got my spore syringes they were clear..i did the shake like hell stuff, but didnt really even show and dispersment..it just looked like water in a syringe..ill wait another week, and see..is it possible that its colonizing the inside of the cake first or is that just a stupid noob theory?


That's possible, if you didn't make any effort to touch the syringe to the glass, when you inoculated, like you are supposed to do:shrug:


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Re: shook brf jars [Re: SomeGuy]
    #14395728 - 05/03/11 06:09 PM (12 years, 8 months ago)

idk but i would imagine that after 2 weeks something should have grown out and be visible. like he said tho the syringe was clear. my lc syringe was clear with white specks in it when i shook it up.


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Re: shook brf jars [Re: lostinbq]
    #14396470 - 05/03/11 07:59 PM (12 years, 8 months ago)

i just looked at my last 3 ccs in the syringe, there were a couple black specs, but not very many..i didnt really attempt to shake for very long this time, my syringe was on the glass everytime, and i watched it run down the sides and all that..i think the moisture inside the brf jars is whats causing the problem..any opinions on that?


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Re: shook brf jars [Re: jremines]
    #14396669 - 05/03/11 08:36 PM (12 years, 8 months ago)

:shrug:


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Re: shook brf jars [Re: lostinbq]
    #14400814 - 05/04/11 04:24 PM (12 years, 8 months ago)

2 cups verm added to 1 cup water, Mix well, then add BRF. Should be fine. Have GE hole(s)?
If your good with a syringe make another batch & see what happens.


Edited by mabachko (05/04/11 04:32 PM)


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