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OfflineJninja
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Registered: 04/07/11
Posts: 4
Last seen: 12 years, 7 months
LC help, i cant seem to find this situation anywhere...
    #14389123 - 05/02/11 03:07 PM (12 years, 8 months ago)

Hello citizens of shroomery and thanks in advance for your help. I'm a long time reader of the message boards and learned pretty much everything i know here, so thank you all for teaching me. i've had a few successful grows in the past but i recently bought a pressure cooker hoping to make my batches cleaner and have less risk of contams. So in my latest batch i boiled some light malt and dextrose (4%) in a pot and put it into three separate olive jars and pc'd them for about 90 minutes. the jar set up is as follows

-approx 12 oz clear glass jars with a metal twist cap
-self healing injection port in lid
- no vent hole because i saw many posts claiming that peoplefind gas exchange is not necessary for LCs
- cooked with lids loose, tightened while still warm/hot
- 1 knocked up with spores from a print, other 2 from syringes, all after the jars had cooled overnight

the problem is that there is no growth of any kind, bacteria or myc from what i can tell. all 3 jars are almost 2 weeks old, shaken multiple times a day and still crystal clear light brownish malt color, with a bit of the sediment in the bottom from cooking.

does anyone know whats going on? it seems like i have no contams or myc and the jars are just kinda hanging out

i apologize for making the post so long, if pics would help, i can post them, thanks in advance to any insight.


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InvisibleDoc_T
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Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: LC help, i cant seem to find this situation anywhere... [Re: Jninja]
    #14389257 - 05/02/11 03:27 PM (12 years, 8 months ago)

LC. :thumbdown:

Go grab a pack of agar for $1.69, and you'll know what's what in a couple days.


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You make it all possible. Doesn't it feel good?


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OfflineJninja
Stranger

Registered: 04/07/11
Posts: 4
Last seen: 12 years, 7 months
Re: LC help, i cant seem to find this situation anywhere... [Re: Jninja]
    #14389465 - 05/02/11 03:57 PM (12 years, 8 months ago)

Thanks i'll check that out, anyone know where i can get agar locally in orlando?

I guess I was just taught to use a LC from a friend and we've always had success (when it wasnt contaminated) so i never really looked back, you're saying i'll have better luck with agar? i'll have to do some more reading i suppose.


Edited by Jninja (05/02/11 04:00 PM)


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InvisibleCarl Sagan
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Registered: 04/19/11
Posts: 922
Loc: Myco-tek.org
Re: LC help, i cant seem to find this situation anywhere... [Re: Jninja]
    #14389484 - 05/02/11 03:59 PM (12 years, 8 months ago)

Quote:

Jninja said:
Thanks i'll check that out, anyone know where i can get agar locally in orlando?



http://www.mycosupply.com/cgi-bin/shopper.cgi?preadd=action&key=MEAR01


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“Sacred cows make the best hamburger”

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Independant Research Foundation


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InvisibleWise Toad
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Registered: 06/08/10
Posts: 2,690
Re: LC help, i cant seem to find this situation anywhere... [Re: Carl Sagan]
    #14389515 - 05/02/11 04:05 PM (12 years, 8 months ago)

I read a while back that a filter is needed on LCs so that the vacuum doesn't suck contaminated air in, not sure if its true tho.

I like GLCs to have lots of innoculant around, I plan to do some with my upcoming clone/isolates


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OfflineJninja
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Registered: 04/07/11
Posts: 4
Last seen: 12 years, 7 months
Re: LC help, i cant seem to find this situation anywhere... [Re: Wise Toad]
    #14389561 - 05/02/11 04:14 PM (12 years, 8 months ago)

I'm starting to agree, Toad. I'm thinking i need to try again and add a tyvek hole to the top as well for gas exchange. i'm thinking that even though gas exchange may not be super important, my jars might be holding their vacuum and be in a kind of "canned" state. Obviously myc will not grow with no oxygen at all, i just hate to trash it and start over.


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InvisibleWise Toad
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Re: LC help, i cant seem to find this situation anywhere... [Re: Jninja]
    #14389623 - 05/02/11 04:22 PM (12 years, 8 months ago)

Honey LC is it? It wouldn't be a vacuum of air unless you tried to withdraw LC before anything grew, even then Im not sure if its devoid of air, just pressure mixmatch. Have you tried GLC yet, they are so :awebig:


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OfflineJninja
Stranger

Registered: 04/07/11
Posts: 4
Last seen: 12 years, 7 months
Re: LC help, i cant seem to find this situation anywhere... [Re: Wise Toad]
    #14389905 - 05/02/11 05:09 PM (12 years, 8 months ago)

I love the face haha, I'm not familiar with GLC though? do you know of a good thread off hand that I can check out to learn more?

edit: why did I put a question mark after "though" in the first sentence? /grammar fail.


Edited by Jninja (05/02/11 05:51 PM)


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