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Invisiblejohn6886
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Mycellium Water Question *DELETED*
    #14387211 - 05/02/11 07:34 AM (12 years, 8 months ago)

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Re: Mycellium Water Question [Re: john6886]
    #14387220 - 05/02/11 07:38 AM (12 years, 8 months ago)

Several months to maybe a year, possibly more.
IF it's perfectly clean and you've stored it correctly.


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Offlinegym
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Re: Mycellium Water Question [Re: john6886]
    #14387226 - 05/02/11 07:40 AM (12 years, 8 months ago)

A liquid culture will last a long time, like several years maybe.....assuming that's what you mean by "Mycellium Water".


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Re: Mycellium Water Question [Re: gym]
    #14387234 - 05/02/11 07:44 AM (12 years, 8 months ago)

Myceliated water is commonly called "Grain LC".
It's a liquid inoculant but not a sugar LC.


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Invisiblejohn6886
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Re: Mycellium Water Question *DELETED* [Re: Doc_T]
    #14387267 - 05/02/11 07:55 AM (12 years, 8 months ago)

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Re: Mycellium Water Question [Re: john6886]
    #14387273 - 05/02/11 07:56 AM (12 years, 8 months ago)

Not ideal, but plausible.
I'd put some spores away as Plan B if I could.


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Re: Mycellium Water Question *DELETED* [Re: Doc_T]
    #14387290 - 05/02/11 08:02 AM (12 years, 8 months ago)

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Re: Mycellium Water Question [Re: john6886]
    #14387298 - 05/02/11 08:03 AM (12 years, 8 months ago)

Quote:

john6886 said: I'm sure there's probably a tek on this but I just kinda made this up just now.




:nonono:


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Re: Mycellium Water Question *DELETED* [Re: Doc_T]
    #14387315 - 05/02/11 08:08 AM (12 years, 8 months ago)

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Re: Mycellium Water Question [Re: john6886]
    #14387340 - 05/02/11 08:14 AM (12 years, 8 months ago)

My point being there are standard methods of preserving cultures.
No need to invent anything, just do it per the tek.

Let me say this: I usually rant about how tap water is perfect for everything.
But for this application, long-term storage of myc fragments, I'd recommend pure distilled water.
And several small syringes as opposed to one big one.
And I'd use syringe caps, not leave the needles on.

And a good stable fridge, if possible.


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Invisiblejohn6886
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Re: Mycellium Water Question *DELETED* [Re: Doc_T]
    #14387351 - 05/02/11 08:18 AM (12 years, 8 months ago)

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Re: Mycellium Water Question [Re: john6886]
    #14387390 - 05/02/11 08:27 AM (12 years, 8 months ago)

Don't wipe anything with alcohol to sterilize it, ever.
The needles and are sterile out of the pack, and can be re-sterilized by pressure cooking.
OF COURSE you will be wearing gloves (at least!) while preparing your master cultures.

I'd put each fresh syringe into a separate new baggie, then squeeze out most but not all the air and put them in another bag.
That gives you a contam barrier and some thermal buffering as well to smooth out the temp compared to the refrigerator cycles.

Edit to re-answer original question:
If you do this perfectly, with a nice healthy sample of myc, clean water, and a good refrigerator, it could very well last a long, long time. Many years.
The biggest risk would be you opening things up to get out a sample.


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Edited by Doc_T (05/02/11 08:32 AM)


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Invisiblejohn6886
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Re: Mycellium Water Question *DELETED* [Re: Doc_T]
    #14387405 - 05/02/11 08:31 AM (12 years, 8 months ago)

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Re: Mycellium Water Question [Re: john6886]
    #14387426 - 05/02/11 08:35 AM (12 years, 8 months ago)

It's an unnecessary risk.
I am going to suggest you handle this in such a way you are not tempted to wipe the caps or the needles.

If you PC the needles, they are sterile right out of the foil, no need for flaming.


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Invisiblejohn6886
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Re: Mycellium Water Question *DELETED* [Re: Doc_T]
    #14387430 - 05/02/11 08:36 AM (12 years, 8 months ago)

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Re: Mycellium Water Question [Re: john6886]
    #14387435 - 05/02/11 08:39 AM (12 years, 8 months ago)

Make prints if you can, like you said.
And if possible, store one syringe somewhere else, a friend's house or whatever.
Then you're protected against power outages, drunk roommates, and acts of dog.


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Invisiblejohn6886
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Re: Mycellium Water Question *DELETED* [Re: Doc_T]
    #14387439 - 05/02/11 08:40 AM (12 years, 8 months ago)

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Re: Mycellium Water Question [Re: john6886]
    #14387570 - 05/02/11 09:23 AM (12 years, 8 months ago)

if you have black soot i assume your using a lighter?
get an alcohol lamp or a gas torch. the flame is cleaner,
no black soot.


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Re: Mycellium Water Question [Re: john6886]
    #14387623 - 05/02/11 09:47 AM (12 years, 8 months ago)

Quote:

john6886 said:
Here's why I was talking about wiping down the cap


http://www.shroomery.org/forums/showflat.php/Number/12098357#12098357




People take unnecessary steps which do nothing but add risk.
Wiping anything with alcohol is not going to sterilize it. Better to just keep it clean in the first place.
Again, you'll have gloves and maybe a mask or glovebox. Why would you need to wipe anything?


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Re: Mycellium Water Question [Re: Doc_T]
    #14387684 - 05/02/11 10:08 AM (12 years, 8 months ago)

Quote:

Doc_T said:
Quote:

john6886 said:
Here's why I was talking about wiping down the cap


http://www.shroomery.org/forums/showflat.php/Number/12098357#12098357




People take unnecessary steps which do nothing but add risk.
Wiping anything with alcohol is not going to sterilize it. Better to just keep it clean in the first place.
Again, you'll have gloves and maybe a mask or glovebox. Why would you need to wipe anything?





I hate to bust in here, but i personally think your theory of wiping down with a sealed alchol wipe a possible contamination to be a bit absurd. The wipes i use are of medical grade, if they are okay be used to wipe wounds, i am pretty sure they are fine to wipe off the black soot from a needle, if it is done within the still air space. I always wipe down my scalpel with one after flaming it. 0% contams.


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Re: Mycellium Water Question [Re: semaphore]
    #14387838 - 05/02/11 10:35 AM (12 years, 8 months ago)

what works works but your introducing an additional
and needless vector for contams. use a clean flame.


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Re: Mycellium Water Question [Re: Fungal growth]
    #14387844 - 05/02/11 10:37 AM (12 years, 8 months ago)

Quote:

Fungal growth said:
what works works but your introducing an additional
and needless vector for contams. use a clean flame.




Okay so by your logic you are implying a alcohol swab packaged in an airtime package, lased with iso is harboring contaminations?


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Re: Mycellium Water Question [Re: semaphore]
    #14387859 - 05/02/11 10:39 AM (12 years, 8 months ago)

Quote:

semaphore said:
Quote:

Fungal growth said:
what works works but your introducing an additional
and needless vector for contams. use a clean flame.




Okay so by your logic you are implying a alcohol swab packaged in an airtime package, lased with iso is harboring contaminations?




almost.

once you open up that "airtime" package, youve now exposed a product advertised as "sterile" to open air. it is no longer sterile. regardless of the alcohol.

the rapid drying of 70% iso alcohol kills most all bacterias.

but has absolutely no effect on most mold spores. i.e. trichoderma.


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Re: Mycellium Water Question [Re: k00laid]
    #14387868 - 05/02/11 10:41 AM (12 years, 8 months ago)

Quote:

k00laid said:
Quote:

semaphore said:
Quote:

Fungal growth said:
what works works but your introducing an additional
and needless vector for contams. use a clean flame.




Okay so by your logic you are implying a alcohol swab packaged in an airtime package, lased with iso is harboring contaminations?




almost.

once you open up that "airtime" package, youve now exposed a product advertised as "sterile" to open air. it is no longer sterile. regardless of the alcohol.

the rapid drying of 70% iso alcohol kills most all bacterias.

but has absolutely no effect on most mold spores. i.e. trichoderma.





Go read my previous post again, i said if you opened it up within your still air environment. Yes obviously if you open up the package outside your glove box or away from your flow hood, then move it to your work environment you will catch bacteria along the way.


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Re: Mycellium Water Question [Re: semaphore]
    #14387882 - 05/02/11 10:43 AM (12 years, 8 months ago)

not probable maybe but entirely possible.
you may NEVER see a contam doing what your doing.
point is, it's possible you might and since it's
simply unneccesary why bother? do you purchase those
swabs? why not just spend the money on a decent flame,
and avoid the risk altogether.


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Offlinek00laid
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Re: Mycellium Water Question [Re: semaphore]
    #14387883 - 05/02/11 10:43 AM (12 years, 8 months ago)

so then is the point of the alcohol swab purely to get the black soot off your needle?

it doesn't bother me much. id rather save money on alcohol pads


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Re: Mycellium Water Question [Re: Fungal growth]
    #14387895 - 05/02/11 10:46 AM (12 years, 8 months ago)

Quote:

Fungal growth said:
not probable maybe but entirely possible.
you may NEVER see a contam doing what your doing.
point is, it's possible you might and since it's
simply unneccesary why bother? do you purchase those
swabs? why not just spend the money on a decent flame,
and avoid the risk altogether.




I used a lighter before getting a torch, but i normally use the swabs to wipe down the utensils.

And at like 2$ for 500 i cant exactly complain that they're expensive.


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Re: Mycellium Water Question *DELETED* [Re: semaphore]
    #14388055 - 05/02/11 11:25 AM (12 years, 8 months ago)

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Re: Mycellium Water Question [Re: john6886]
    #14388205 - 05/02/11 12:03 PM (12 years, 8 months ago)

You guys think too much.
PC your tools, then there's no need for flame.
No fire hazard, no soot, no cost, no danger.


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Re: Mycellium Water Question *DELETED* [Re: Doc_T]
    #14388378 - 05/02/11 12:37 PM (12 years, 8 months ago)

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Re: Mycellium Water Question [Re: john6886]
    #14388385 - 05/02/11 12:38 PM (12 years, 8 months ago)

About every couple of months, somebody sets a glovebox on fire or has an explosion inside one.


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Re: Mycellium Water Question *DELETED* [Re: Doc_T]
    #14388395 - 05/02/11 12:40 PM (12 years, 8 months ago)

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Re: Mycellium Water Question [Re: john6886]
    #14388441 - 05/02/11 12:50 PM (12 years, 8 months ago)

Poor systems engineering, not negligence.
Why introduce an opportunity for human error that you don't need?
Flaming brings no benefit over other sterilization methods, other than being immediate.
Think and plan, you can avoid the need for fire and avoid the need for vigilance around fire.


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Re: Mycellium Water Question [Re: Doc_T]
    #14388725 - 05/02/11 01:46 PM (12 years, 8 months ago)

Quote:

Doc_T said:
About every couple of months, somebody sets a glovebox on fire or has an explosion inside one.





*Raises hand* i had one two nights ago lol i got no harm hairs muwahah


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Re: Mycellium Water Question [Re: semaphore]
    #14388760 - 05/02/11 01:54 PM (12 years, 8 months ago)

i melted a hole in the top of mine.


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Re: Mycellium Water Question [Re: Fungal growth]
    #14396837 - 05/03/11 09:06 PM (12 years, 8 months ago)

lmao i think its safe to say you should follow doc_t's advice. its not how i've been doing it, but since i read this i realized something about sterile technique. now i just gotta find something else to use my blowtorch on...


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