Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinezzantium2999
Shroom Groomer
Male User Gallery


Registered: 03/22/11
Posts: 177
Loc: Toronto Canada Flag
Last seen: 12 years, 2 months
live tisse in agar
    #14377211 - 04/30/11 11:50 AM (12 years, 8 months ago)

i want to make sure this is right before ripping apart a nice  mushy. I can keep a sample that will  ccolonise a ful petri dish by putting a small peice of live tissue in potato agar....right?


--------------------
A good friend would bail you out of jail, but your best friend would be the one sitting next to you saying, "damn that was awesome".


Extras: Filter Print Post Top
OfflineMOPE
Walking Chemical Reaction


Registered: 10/07/08
Posts: 831
Last seen: 10 years, 4 months
Re: live tisse in agar [Re: zzantium2999]
    #14377273 - 04/30/11 12:04 PM (12 years, 8 months ago)

yup, the myc will spread from the live tissue.  to make sure you get a clean sample, I would get an 18 gauge needle, rub down a small section with iso alcohol, and do a little biopsy.  Then just pop that sucka into your petri or w/e


--------------------
Got used to the feeling of falling
But you'll never see me following
Bouncing back and forth between the healing and the hollering
Riding the outer ring of my own private saturn
Thoughts scattered all across the grey matter


Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: live tisse in agar [Re: MOPE]
    #14377346 - 04/30/11 12:16 PM (12 years, 8 months ago)

Needle biopsy is over complicated.
Split the stem, pick out a bit of fiber, drop in a dish.


--------------------
You make it all possible. Doesn't it feel good?


Extras: Filter Print Post Top
OfflineMOPE
Walking Chemical Reaction


Registered: 10/07/08
Posts: 831
Last seen: 10 years, 4 months
Re: live tisse in agar [Re: Doc_T]
    #14377353 - 04/30/11 12:18 PM (12 years, 8 months ago)

Quote:

Doc_T said:
Needle biopsy is over complicated.
Split the stem, pick out a bit of fiber, drop in a dish.




only takes an extra 30 seconds if you already have a thick needle, lol


--------------------
Got used to the feeling of falling
But you'll never see me following
Bouncing back and forth between the healing and the hollering
Riding the outer ring of my own private saturn
Thoughts scattered all across the grey matter


Extras: Filter Print Post Top
Offlinek00laid
NEMO
 User Gallery

Registered: 05/03/10
Posts: 19,636
Last seen: 6 months, 5 days
Re: live tisse in agar [Re: MOPE]
    #14377480 - 04/30/11 12:39 PM (12 years, 8 months ago)

but what is the benefit?


--------------------
AMU - AMU Q & A - MyVideo Teks!


Extras: Filter Print Post Top
OfflineMOPE
Walking Chemical Reaction


Registered: 10/07/08
Posts: 831
Last seen: 10 years, 4 months
Re: live tisse in agar [Re: k00laid]
    #14377531 - 04/30/11 12:50 PM (12 years, 8 months ago)

Quote:

k00laid said:
but what is the benefit?




like i said earlier, a cleaner tissue sample... I know you can remove the clean chunks from a contaminated petri, but wouldnt you rather just have a clean agar culture? 

Plus its super simple, and almost easier than just breaking open the stipe of a fresh shroom to pull fibers out of...


--------------------
Got used to the feeling of falling
But you'll never see me following
Bouncing back and forth between the healing and the hollering
Riding the outer ring of my own private saturn
Thoughts scattered all across the grey matter


Extras: Filter Print Post Top
Invisiblecc2
Mush

Registered: 05/15/10
Posts: 2,611
Re: live tisse in agar [Re: MOPE]
    #14377542 - 04/30/11 12:53 PM (12 years, 8 months ago)

cloneufc's easy cloning tek is even simpler and less contam prone http://www.shroomery.org/forums/showflat.php/Number/12032286#12032286 :headbang:


Extras: Filter Print Post Top
Offlinek00laid
NEMO
 User Gallery

Registered: 05/03/10
Posts: 19,636
Last seen: 6 months, 5 days
Re: live tisse in agar [Re: MOPE]
    #14377543 - 04/30/11 12:53 PM (12 years, 8 months ago)

Quote:

MOPE said:
Quote:

k00laid said:
but what is the benefit?




like i said earlier, a cleaner tissue sample... I know you can remove the clean chunks from a contaminated petri, but wouldnt you rather just have a clean agar culture? 

Plus its super simple, and almost easier than just breaking open the stipe of a fresh shroom to pull fibers out of...




you arent quite getting it.

you pick a nice large mushroom out of your fruiting chamber right?
that mushroom has been in there a few days, being constantly exposed to fresh air.  and as we all know fresh air has the possibility of mold spores, bacteria and the like.


so what happens when you do your needle biopsy straight ontop of a harmless trichoderma spore that has no chance of germinating ON your fruit.  well you put that contam'd sample into your agar or straight into your LC where it is doomed to fail.



when splitting the stem lengthwise in a still air box or in front of a flowhood, you are selecting a sample of tissue that has NEVER been exposed to any air. except that of your still air box or floowhood.



tldr; needle biopsies are NOt going to provide a "cleaner" sample than a mid-stem tissue sample would.


--------------------
AMU - AMU Q & A - MyVideo Teks!


Extras: Filter Print Post Top
OfflineMOPE
Walking Chemical Reaction


Registered: 10/07/08
Posts: 831
Last seen: 10 years, 4 months
Re: live tisse in agar [Re: k00laid]
    #14377581 - 04/30/11 01:02 PM (12 years, 8 months ago)

Quote:

k00laid said:
Quote:

MOPE said:
Quote:

k00laid said:
but what is the benefit?




like i said earlier, a cleaner tissue sample... I know you can remove the clean chunks from a contaminated petri, but wouldnt you rather just have a clean agar culture? 

Plus its super simple, and almost easier than just breaking open the stipe of a fresh shroom to pull fibers out of...




you arent quite getting it.

you pick a nice large mushroom out of your fruiting chamber right?
that mushroom has been in there a few days, being constantly exposed to fresh air.  and as we all know fresh air has the possibility of mold spores, bacteria and the like.


so what happens when you do your needle biopsy straight ontop of a harmless trichoderma spore that has no chance of germinating ON your fruit.  well you put that contam'd sample into your agar or straight into your LC where it is doomed to fail.



when splitting the stem lengthwise in a still air box or in front of a flowhood, you are selecting a sample of tissue that has NEVER been exposed to any air. except that of your still air box or floowhood.



tldr; needle biopsies are NOt going to provide a "cleaner" sample than a mid-stem tissue sample would.





believe me, i do get it, thats why I mentioned that I wipe down the outside of the biopsy area with iso before hand, to wipe away mold spores/kill bacteria before i take the biopsy. 

Maybe we have different methods, but I have an extremely low contam rate, and this way works well for me.

Edit:  Forgot to add, when OP asked his question, he just said he was going to tear a piece of the fruit off and put it in a petri, and the method I described was cleaner than what he was going to do (didn't think about pullin a piece from the inside of the stipe since he didnt mention it)


--------------------
Got used to the feeling of falling
But you'll never see me following
Bouncing back and forth between the healing and the hollering
Riding the outer ring of my own private saturn
Thoughts scattered all across the grey matter


Edited by MOPE (04/30/11 01:06 PM)


Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: live tisse in agar [Re: k00laid]
    #14377647 - 04/30/11 01:16 PM (12 years, 8 months ago)

Quote:

k00laid said:
tldr; needle biopsies are NOt going to provide a "cleaner" sample than a mid-stem tissue sample would.




This. It's more work for no benefit.
Plus the risk of jabbing your thumb with a needle, for reals.


--------------------
You make it all possible. Doesn't it feel good?


Extras: Filter Print Post Top
OfflineMOPE
Walking Chemical Reaction


Registered: 10/07/08
Posts: 831
Last seen: 10 years, 4 months
Re: live tisse in agar [Re: Doc_T]
    #14377703 - 04/30/11 01:26 PM (12 years, 8 months ago)

Quote:

Doc_T said:
It's more the same amount of work for no benefit the same results.
Plus the risk of jabbing your thumb with a needle, for reals.




fixed

and lolz


--------------------
Got used to the feeling of falling
But you'll never see me following
Bouncing back and forth between the healing and the hollering
Riding the outer ring of my own private saturn
Thoughts scattered all across the grey matter


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* buying agar - help sinisha 3,417 6 03/31/06 05:49 AM
by FractalXplora
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 7,382 8 02/14/05 07:57 AM
by Roadkill
* Potato Dextrose Agar X Malt Extract Agar Alegalize 6,156 8 02/25/05 11:03 AM
by agar
* test tubes agar fungophiliac 3,135 8 06/19/20 09:41 PM
by gorilliam
* LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! agentstone 4,593 12 03/30/20 10:37 AM
by Surf707
* Agar and the global economy Dem_Bones 1,650 4 03/01/06 09:08 AM
by Dem_Bones
* Agar question WormholeSurfer 1,485 3 10/29/05 07:10 PM
by agar
* Agar's Home Made Compost recipe for bulk substrates
( 1 2 3 4 all )
agar 20,885 75 02/12/21 05:50 AM
by Bart84

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
474 topic views. 29 members, 195 guests and 49 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.01 seconds on 14 queries.