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zzantium2999
Shroom Groomer



Registered: 03/22/11
Posts: 177
Loc: Toronto Canada
Last seen: 12 years, 2 months
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live tisse in agar
#14377211 - 04/30/11 11:50 AM (12 years, 8 months ago) |
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i want to make sure this is right before ripping apart a nice mushy. I can keep a sample that will ccolonise a ful petri dish by putting a small peice of live tissue in potato agar....right?
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A good friend would bail you out of jail, but your best friend would be the one sitting next to you saying, "damn that was awesome".
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MOPE
Walking Chemical Reaction


Registered: 10/07/08
Posts: 831
Last seen: 10 years, 4 months
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yup, the myc will spread from the live tissue. to make sure you get a clean sample, I would get an 18 gauge needle, rub down a small section with iso alcohol, and do a little biopsy. Then just pop that sucka into your petri or w/e
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Got used to the feeling of falling But you'll never see me following Bouncing back and forth between the healing and the hollering Riding the outer ring of my own private saturn Thoughts scattered all across the grey matter
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: live tisse in agar [Re: MOPE]
#14377346 - 04/30/11 12:16 PM (12 years, 8 months ago) |
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Needle biopsy is over complicated. Split the stem, pick out a bit of fiber, drop in a dish.
-------------------- You make it all possible. Doesn't it feel good?
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MOPE
Walking Chemical Reaction


Registered: 10/07/08
Posts: 831
Last seen: 10 years, 4 months
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Re: live tisse in agar [Re: Doc_T]
#14377353 - 04/30/11 12:18 PM (12 years, 8 months ago) |
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Quote:
Doc_T said: Needle biopsy is over complicated. Split the stem, pick out a bit of fiber, drop in a dish.
only takes an extra 30 seconds if you already have a thick needle, lol
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Got used to the feeling of falling But you'll never see me following Bouncing back and forth between the healing and the hollering Riding the outer ring of my own private saturn Thoughts scattered all across the grey matter
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k00laid
NEMO


Registered: 05/03/10
Posts: 19,636
Last seen: 6 months, 5 days
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Re: live tisse in agar [Re: MOPE]
#14377480 - 04/30/11 12:39 PM (12 years, 8 months ago) |
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but what is the benefit?
-------------------- AMU - AMU Q & A - MyVideo Teks!
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MOPE
Walking Chemical Reaction


Registered: 10/07/08
Posts: 831
Last seen: 10 years, 4 months
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Re: live tisse in agar [Re: k00laid]
#14377531 - 04/30/11 12:50 PM (12 years, 8 months ago) |
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Quote:
k00laid said: but what is the benefit?
like i said earlier, a cleaner tissue sample... I know you can remove the clean chunks from a contaminated petri, but wouldnt you rather just have a clean agar culture?
Plus its super simple, and almost easier than just breaking open the stipe of a fresh shroom to pull fibers out of...
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Got used to the feeling of falling But you'll never see me following Bouncing back and forth between the healing and the hollering Riding the outer ring of my own private saturn Thoughts scattered all across the grey matter
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cc2
Mush

Registered: 05/15/10
Posts: 2,611
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Re: live tisse in agar [Re: MOPE]
#14377542 - 04/30/11 12:53 PM (12 years, 8 months ago) |
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k00laid
NEMO


Registered: 05/03/10
Posts: 19,636
Last seen: 6 months, 5 days
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Re: live tisse in agar [Re: MOPE]
#14377543 - 04/30/11 12:53 PM (12 years, 8 months ago) |
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Quote:
MOPE said:
Quote:
k00laid said: but what is the benefit?
like i said earlier, a cleaner tissue sample... I know you can remove the clean chunks from a contaminated petri, but wouldnt you rather just have a clean agar culture?
Plus its super simple, and almost easier than just breaking open the stipe of a fresh shroom to pull fibers out of...
you arent quite getting it.
you pick a nice large mushroom out of your fruiting chamber right? that mushroom has been in there a few days, being constantly exposed to fresh air. and as we all know fresh air has the possibility of mold spores, bacteria and the like.
so what happens when you do your needle biopsy straight ontop of a harmless trichoderma spore that has no chance of germinating ON your fruit. well you put that contam'd sample into your agar or straight into your LC where it is doomed to fail.
when splitting the stem lengthwise in a still air box or in front of a flowhood, you are selecting a sample of tissue that has NEVER been exposed to any air. except that of your still air box or floowhood.
tldr; needle biopsies are NOt going to provide a "cleaner" sample than a mid-stem tissue sample would.
-------------------- AMU - AMU Q & A - MyVideo Teks!
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MOPE
Walking Chemical Reaction


Registered: 10/07/08
Posts: 831
Last seen: 10 years, 4 months
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Re: live tisse in agar [Re: k00laid]
#14377581 - 04/30/11 01:02 PM (12 years, 8 months ago) |
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Quote:
k00laid said:
Quote:
MOPE said:
Quote:
k00laid said: but what is the benefit?
like i said earlier, a cleaner tissue sample... I know you can remove the clean chunks from a contaminated petri, but wouldnt you rather just have a clean agar culture?
Plus its super simple, and almost easier than just breaking open the stipe of a fresh shroom to pull fibers out of...
you arent quite getting it.
you pick a nice large mushroom out of your fruiting chamber right? that mushroom has been in there a few days, being constantly exposed to fresh air. and as we all know fresh air has the possibility of mold spores, bacteria and the like.
so what happens when you do your needle biopsy straight ontop of a harmless trichoderma spore that has no chance of germinating ON your fruit. well you put that contam'd sample into your agar or straight into your LC where it is doomed to fail.
when splitting the stem lengthwise in a still air box or in front of a flowhood, you are selecting a sample of tissue that has NEVER been exposed to any air. except that of your still air box or floowhood.
tldr; needle biopsies are NOt going to provide a "cleaner" sample than a mid-stem tissue sample would.
believe me, i do get it, thats why I mentioned that I wipe down the outside of the biopsy area with iso before hand, to wipe away mold spores/kill bacteria before i take the biopsy.
Maybe we have different methods, but I have an extremely low contam rate, and this way works well for me.
Edit: Forgot to add, when OP asked his question, he just said he was going to tear a piece of the fruit off and put it in a petri, and the method I described was cleaner than what he was going to do (didn't think about pullin a piece from the inside of the stipe since he didnt mention it)
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Got used to the feeling of falling But you'll never see me following Bouncing back and forth between the healing and the hollering Riding the outer ring of my own private saturn Thoughts scattered all across the grey matter
Edited by MOPE (04/30/11 01:06 PM)
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: live tisse in agar [Re: k00laid]
#14377647 - 04/30/11 01:16 PM (12 years, 8 months ago) |
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Quote:
k00laid said: tldr; needle biopsies are NOt going to provide a "cleaner" sample than a mid-stem tissue sample would.
This. It's more work for no benefit. Plus the risk of jabbing your thumb with a needle, for reals.
-------------------- You make it all possible. Doesn't it feel good?
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MOPE
Walking Chemical Reaction


Registered: 10/07/08
Posts: 831
Last seen: 10 years, 4 months
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Re: live tisse in agar [Re: Doc_T]
#14377703 - 04/30/11 01:26 PM (12 years, 8 months ago) |
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Quote:
Doc_T said: It's more the same amount of work for no benefit the same results. Plus the risk of jabbing your thumb with a needle, for reals.
fixed
and lolz
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Got used to the feeling of falling But you'll never see me following Bouncing back and forth between the healing and the hollering Riding the outer ring of my own private saturn Thoughts scattered all across the grey matter
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