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OfflineCaptainAhab


Registered: 12/25/09
Posts: 1,875
Last seen: 5 years, 11 months
RR's Cubensis-Panaeolus Hybrid: Any News?
    #14375773 - 04/30/11 01:34 AM (12 years, 8 months ago)

In these threads that I found from September of 2008, I saw that RR was working on a method to hybridize different species. He was successful in creating a Panaeolus-Cubensis hybrid that fruited, and then all the news stopped. All the info that I have is posted, below.

Does anyone know if there was ever any news about the paper he planned to write, or the methods he was trying?

Did RR ever pass out prints of the species hybrid he allegedly fruited?

Are there any pictures of the alleged fruit? And if so, did anyone eat any? lol

--> I bolded/colored the section of interest, and pasted the rest. It's a pretty cool technique.

I've started another thread on attempting a strain-hybridization (not species hybridization), which you can see here:
http://www.shroomery.org/forums/showflat.php/Number/14372553

Thanks!


Species Hybridization
http://www.shroomery.org/forums/showflat.php/Number/8967425#8967425
"Yes, it's very difficult and out of hundreds of experiments, this is the only one that crossed. A nearly lethal dose of rattlesnake venom was used in the agar. I have pictures, but they're going into a paper I'll be writing for publication in the IJMM when I get time, possibly this winter.

I'm also working on another hybridization technique that is showing early promise.  If it works, I'll post the results.  If not, this is the last you'll hear of it.  I don't post ideas or failures.
RR"

"That's not how you identify hybrids.  They're a distinct sector on a petri dish that is separate from either parent.

For example, in the petri dish below, small samples of two species were placed about 1 inch apart on the same petri dish of venomated agar.  They were Pan cyan on the right, and P cubensis on the left.  As you can see, when the two cultures met, a third sector grew from the meeting point.  This sector was a hybrid of the two species.  Actually, they were of different genera according to mycologists who classify such things.



The fruits grown from this culture displayed properties of both species.
RR"

"It's from a few years ago, immediately after working on the Redboy strain.  I made literally hundreds of cross-species attempts before getting this one.  I'm fairly certain I sent pictures to John Allen for verification on the ID at the time, but he's the only one.  I still have the culture though and will eventually get it back out.  Once I get the paper written up, I'll release it.  Until then, I'd prefer to keep it low key.

You can clearly see the third sector that grew from the 12 o'clock position where the two species met. Those of you who are familiar with the looks of pan cyan and cubensis mycelium will clearly see the two in that picture.

I have another technique that was showing much better results than snake venom in crossing otherwise non-compatible strains, so if it works to create interspecies hybrids, I'll get a paper written on it.


Edited by CaptainAhab (04/30/11 02:07 AM)


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InvisibleLuger0815
noob
Male

Registered: 12/21/10
Posts: 1,677
Loc: @ Home
Re: RR's Cubensis-Panaeolus Hybrid: Any News? [Re: CaptainAhab]
    #14375893 - 04/30/11 02:30 AM (12 years, 8 months ago)

:popcorn:


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OfflineLarrythescaryrex
teardrop on the fire
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Registered: 07/19/00
Posts: 11,004
Loc: further down the spiral
Last seen: 1 year, 1 month
Re: RR's Cubensis-Panaeolus Hybrid: Any News? [Re: Luger0815]
    #14376062 - 04/30/11 04:08 AM (12 years, 8 months ago)

:tinfoil: perhaps we shouldn't ask to many questions...


--------------------
RIP Acidic_Sloth

Sunset_Mission said:
"larry the scary rex
verily scary when thoroughly vexed
invoke the shadows and dust, cast a hex
mercifully massacring memories masterfully
relocate from Ur to 8th density and become a cosmic bully
mulder and scully couldn't decipher his glyphs
invoke the shadows and dust, smoke infernal spliffs"
April 24th 2011


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Offlinebig duder
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Registered: 01/03/10
Posts: 29
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Re: RR's Cubensis-Panaeolus Hybrid: Any News? [Re: Larrythescaryrex]
    #14376587 - 04/30/11 09:09 AM (12 years, 8 months ago)

Frankin shrooms, YUM!!


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Offlinepunkrocker292004
i am a liar
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Registered: 12/17/09
Posts: 2,921
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Re: RR's Cubensis-Panaeolus Hybrid: Any News? [Re: big duder] * 1
    #14376597 - 04/30/11 09:13 AM (12 years, 8 months ago)

:grin:rr should not be holdin back on this we need prints :2cents:


--------------------
EVERYTHING YOU NEED TO KNOW

on a long enough timeline the survival rate for everyone drops to zero-tyler durden

watch me


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Onlinepslyke
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Registered: 06/12/10
Posts: 4,094
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Re: RR's Cubensis-Panaeolus Hybrid: Any News? [Re: punkrocker292004]
    #14376757 - 04/30/11 10:01 AM (12 years, 8 months ago)

I am very interested in the outcome of these experiments as well. I am not sure that I understand the need to use venomated agar. Is this a common approach in mycology? There are several agents that I am familiar with that promote cell fusion (at least in eukaryotic mammalian cells). For instance, polyethylene glycol is a mainstay in producing fused cells. Has anyone ever tried this approach for mycology?

http://www.springerlink.com/content/l16u317084334h30/#section=81823&page=1&locus=0

Another approach utilizes Sendai virus. Finally, cationic lipids might be something else to consider for those attempting to create hybrids. In the case of mycology it may be necessary to first partially break down the outer cell wall with a gentle enzymatic digestion. If anyone is interested in such approaches pm me.


--------------------
"What appears impenetrable to us does exist, manifesting itself in the deepest wisdom and the most radiant beauty" Einstein

"The conservatives of 70 years ago would be outraged at what has come to pass. It embodies everything they took up arms for to defeat"Asante


:kratom:


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OfflineRogerRabbitM
Bans for Pleasure
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Registered: 03/26/03
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Re: RR's Cubensis-Panaeolus Hybrid: Any News? [Re: CaptainAhab]
    #14377032 - 04/30/11 10:01 AM (12 years, 8 months ago)

This thread has been closed.

Reason:
Quote:

RogerRabbit said:
Until then, I'd prefer to keep it low key.
RR





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